pCambia1301-GUS-bk1

LOCUS      pCambia1301-GUS-bk1         11086 bp DNA     circular SYN 09-APR-2026
DEFINITION  1301.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             417..434
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      469..721
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    743..767
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2068..2697
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3132..4199
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4265..4459
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4803..4943
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5129..5717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5804..6598)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7023..7047
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7125..7299
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7340..8365)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8432..9108)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    9299..9320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9335..9365
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9373..9389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9397..9413
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9409..9421
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MT*L"
     misc_feature    9422..9473
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             9453..9473
                     /codon_start=1
                     /label=Feature 24
                     /translation="GTRYRFK"
     CDS             join(9474..9488,9679..11086,1..410)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          9489..9678
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     misc_feature    complement(9589..9612)
                     /label=p-1305.1-R
ORIGIN
        1 gttattacgg atggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
       61 aagaacttct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
      121 tggatacgtt agccgggctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
      181 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
      241 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
      301 agaaagggat cttcactcgc gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac
      361 gctggactgg catgaacttc ggtgaaaaac cgcagcaggg aggcaaacaa gctagccacc
      421 accaccacca ccacgtgtga attacaggtg accagctcga atttccccga tcgttcaaac
      481 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
      541 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
      601 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
      661 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
      721 cgggaattaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
      781 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
      841 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
      901 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
      961 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     1021 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     1081 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     1141 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     1201 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     1261 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     1321 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     1381 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     1441 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     1501 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     1561 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     1621 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     1681 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     1741 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     1801 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     1861 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     1921 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     1981 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     2041 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     2101 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     2161 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     2221 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     2281 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     2341 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     2401 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     2461 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     2521 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     2581 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     2641 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     2701 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     2761 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     2821 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     2881 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     2941 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     3001 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     3061 gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     3121 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     3181 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     3241 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     3301 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     3361 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     3421 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     3481 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     3541 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     3601 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     3661 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     3721 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     3781 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     3841 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     3901 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     3961 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     4021 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     4081 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     4141 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     4201 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     4261 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     4321 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     4381 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     4441 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     4501 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     4561 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     4621 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     4681 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     4741 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     4801 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     4861 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     4921 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4981 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5041 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5101 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5161 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5221 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5281 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5341 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5401 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5461 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5521 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     5581 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5641 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5701 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5761 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     5821 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     5881 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     5941 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     6001 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     6061 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     6121 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     6181 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     6241 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     6301 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     6361 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     6421 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     6481 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     6541 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     6601 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     6661 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     6721 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     6781 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
     6841 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
     6901 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
     6961 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     7021 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     7081 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     7141 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     7201 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
     7261 cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgacag
     7321 atcccggtcg gcatctactc tatttctttg ccctcggacg agtgctgggg cgtcggtttc
     7381 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     7441 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     7501 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     7561 tgcggagcat atacgcccgg agtcgtggcg atcctgcaag ctccggatgc ctccgctcga
     7621 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     7681 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     7741 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
     7801 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
     7861 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
     7921 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
     7981 ggctaagatc ggccgcagcg atcgcatcca tagcctccgc gaccggttgt agaacagcgg
     8041 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgaacggcgg gagatgcaat
     8101 aggtcaggct ctcgctaaac tccccaatgt caagcacttc cggaatcggg agcgcggccg
     8161 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
     8221 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
     8281 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
     8341 tcgcggtgag ttcaggcttt ttcatatctc attgcccccc ggatctgcga aagctcgaga
     8401 gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac
     8461 ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
     8521 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     8581 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     8641 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     8701 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     8761 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     8821 tcttggagta gacgagagtg tcgtgctcca ccatgttcac atcaatccac ttgctttgaa
     8881 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
     8941 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
     9001 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
     9061 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
     9121 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
     9181 ttggcaagct gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9241 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9301 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     9361 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     9421 cgaattcgag ctcggtcgac tctcgaggat ccggtacccg gtatcgattt aaaatggtag
     9481 atctgagggt aaatttctag tttttctcct tcattttctt ggttaggacc cttttctctt
     9541 tttatttttt tgagctttga tctttcttta aactgatcta ttttttaatt gattggttat
     9601 ggtgtaaata ttacatagct ttaactgata atctgattac tttatttcgt gtgtctatga
     9661 tgatgatgat agttacagaa ccgacgactc gtccgtcctg tagaaacccc aacccgtgaa
     9721 atcaaaaaac tcgacggcct gtgggcattc agtctggatc gcgaaaactg tggaattgat
     9781 cagcgttggt gggaaagcgc gttacaagaa agccgggcaa ttgctgtgcc aggcagtttt
     9841 aacgatcagt tcgccgatgc agatattcgt aattatgcgg gcaacgtctg gtatcagcgc
     9901 gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg tgctgcgttt cgatgcggtc
     9961 actcattacg gcaaagtgtg ggtcaataat caggaagtga tggagcatca gggcggctat
    10021 acgccatttg aagccgatgt cacgccgtat gttattgccg ggaaaagtgt acgtatcacc
    10081 gtttgtgtga acaacgaact gaactggcag actatcccgc cgggaatggt gattaccgac
    10141 gaaaacggca agaaaaagca gtcttacttc catgatttct ttaactatgc cggaatccat
    10201 cgcagcgtaa tgctctacac cacgccgaac acctgggtgg acgatatcac cgtggtgacg
    10261 catgtcgcgc aagactgtaa ccacgcgtct gttgactggc aggtggtggc caatggtgat
    10321 gtcagcgttg aactgcgtga tgcggatcaa caggtggttg caactggaca aggcactagc
    10381 gggactttgc aagtggtgaa tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa
    10441 ctgtgcgtca cagccaaaag ccagacagag tgtgatatct acccgcttcg cgtcggcatc
    10501 cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc acaaaccgtt ctactttact
    10561 ggctttggtc gtcatgaaga tgcggacttg cgtggcaaag gattcgataa cgtgctgatg
    10621 gtgcacgacc acgcattaat ggactggatt ggggccaact cctaccgtac ctcgcattac
    10681 ccttacgctg aagagatgct cgactgggca gatgaacatg gcatcgtggt gattgatgaa
    10741 actgctgctg tcggctttaa cctctcttta ggcattggtt tcgaagcggg caacaagccg
    10801 aaagaactgt acagcgaaga ggcagtcaac ggggaaactc agcaagcgca cttacaggcg
    10861 attaaagagc tgatagcgcg tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc
    10921 aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt tcgcgccact ggcggaagca
    10981 acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt ctgcgacgct
    11041 cacaccgata ccatcagcga tctctttgat gtgctgtgcc tgaacc
//