pCAMBIA1301-proMpEF1a-GFP-MpKAN

LOCUS      pCAMBIA1301-proMpEF1a-GFP-MpKAN             17041 bp DNA     circular SYN 03-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..17041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      23..275
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    297..321
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1621..2250
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2679..3752
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3818..4012
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4356..4496
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4682..5270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5357..6151)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6576..6600
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6678..6852
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6892..7917)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(7985..8662)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8853..8874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8889..8919
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8927..8943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8951..8967
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8983..10711
                     /label=proMpEF1alpha
     CDS             10727..11443
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            11450..17020
                     /label=Mp3g04970
ORIGIN
        1 ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
       61 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
      121 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
      181 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
      241 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttaaactatc agtgtttgac
      301 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa cggatattta
      361 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
      421 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
      481 gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
      541 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
      601 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
      661 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
      721 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
      781 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
      841 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
      901 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
      961 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     1021 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     1081 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     1141 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     1201 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     1261 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     1321 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     1381 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca
     1441 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     1501 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     1561 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     1621 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     1681 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     1741 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     1801 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     1861 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     1921 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     1981 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     2041 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     2101 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     2161 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     2221 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     2281 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     2341 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     2401 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     2461 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     2521 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     2581 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc
     2641 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     2701 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     2761 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     2821 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     2881 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     2941 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     3001 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     3061 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     3121 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     3181 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     3241 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     3301 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     3361 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     3421 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     3481 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     3541 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     3601 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     3661 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     3721 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     3781 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     3841 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     3901 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     3961 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     4021 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     4081 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     4141 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     4201 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     4261 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     4321 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     4381 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     4441 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     4501 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4561 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4621 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4681 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4741 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4801 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4861 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4921 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4981 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5041 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5101 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     5161 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5221 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5281 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5341 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
     5401 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
     5461 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
     5521 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
     5581 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
     5641 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
     5701 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
     5761 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
     5821 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
     5881 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
     5941 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
     6001 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
     6061 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
     6121 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
     6181 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
     6241 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
     6301 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
     6361 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
     6421 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
     6481 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
     6541 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     6601 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
     6661 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
     6721 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
     6781 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
     6841 attatggaga aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt
     6901 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     6961 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     7021 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     7081 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg
     7141 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     7201 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     7261 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     7321 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     7381 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     7441 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     7501 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     7561 catagcctcc gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     7621 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat
     7681 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     7741 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     7801 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     7861 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
     7921 tcattgcccc ccgggatctg cgaaagctcg agagagatag atttgtagag agagactggt
     7981 gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga
     8041 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
     8101 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     8161 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     8221 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     8281 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     8341 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     8401 caccatgtta tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
     8461 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
     8521 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
     8581 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
     8641 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
     8701 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
     8761 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8821 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8881 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     8941 caatttcaca caggaaacag ctatgaccat gattacgaat tccaaatgag tcacacacat
     9001 tgttgagaga catatcaaaa taactcaatt gtaaggtaag ttgatagatt tcccagttaa
     9061 atgcattatt ttaacaatat ttgtatctca aattttcaaa atcatatcaa atttgcagat
     9121 cttgatgatg attcacaact ctgtcttcga tttttgattg ttaacgggta tcgatatttg
     9181 ggtaacttaa aaagaatttg ctaaagatct taaaaaacag attaaaaaaa cctttcaaaa
     9241 tagctaatcc tttaaatccg tttcacaata taatgctcat tgatgataat cgaaaagatt
     9301 ccattttaaa gtgcatataa attgcttaat tatctctttc tgtctcgtaa tattttgttc
     9361 tctcctattg gatcggaccc gcatcttctc gatcagttca ctatactaat atttggtcaa
     9421 cttcaagaga gattagaact tcgttctcca aagtgtaaag gccgacaagc agaaaaggaa
     9481 acttcaatca gaagccttct aggttttccc gcgtcccaaa aagcgaaggg aggacgggga
     9541 ggacggtgag gccagaagaa gaaagcaaca gtaaaatctc atgtgattgc gtgcatgtca
     9601 tggatctatg ggatgctagt ccaccgtttg gaagtatgtg cagaaggaga aaggcgcggg
     9661 aggccaaaag cgacttcggc cacctctcta ctttctagaa agtttgctcg cagcttgtaa
     9721 taagcgcgga aaaggtactc gaaaaaccgg agcaatcggt gtggtatcag cagtcgggtt
     9781 cgctaaacct acgtcttctt attttggaat gttaaattga aagggcgtgc cggccctaag
     9841 cacggacgat gtgacagacg gaggagtgtg agcaattcgg atgacgatgc tggccggcag
     9901 tcacgacaaa gagtccgaag gggcatggac agggcaatat ataaatggaa aagaggcttt
     9961 gaatattcct aacccttcgt cgtcggcctc ccaacttgcc ctcgcatgct gaattggccg
    10021 cctatctgcg aatggccggc cgccgaccct aagtcgcaat aacttgaacg gagccgatgc
    10081 aaagtcctta tcgaacgctc gttattataa acggggcaga cctaggtatt taattatatc
    10141 aaaaccctcc gcaatttcgc taatcattcc ctaatttctt ctactttggc gtctagtgct
    10201 gtggtcgcgc ttgctctcaa agctcgctgg gaggcagtgg agcgtctggc ttaaaatcga
    10261 tcgccgcaac cctagagttc ctttcacttc tttctctctt gcgtggagag catcgacatc
    10321 gaagagctgc atcttgtctt ctggtatgcg ccttgtgccc tcgtctctgt acgtcgttac
    10381 gattgctcga tattgtgatt gtctagacct gaaacccgac tggagctttt acgtggtgtc
    10441 gattggggcc tcgagcgagt gattttttag gttcgtttta gccaagtaca tcccactttg
    10501 gtcagtcctg ttatctcggt aaggtgttca gttcggtctc tggtgcggag gtgcctcgta
    10561 agtttcctgc atgatagatt tgtctatcct tgacttgaag aggagtcgaa ggtcagcgag
    10621 gttggctgtg tgctatggac gtcggtgctc cttggttccg tgcttctatg ggtgttgcct
    10681 caacagtatt gatgtgcctg cagaaaggtt gggtaccgaa aagcttatgg tgagcaaggg
    10741 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
    10801 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
    10861 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
    10921 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
    10981 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
    11041 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
    11101 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
    11161 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
    11221 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
    11281 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
    11341 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
    11401 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggatcca tgaaaatgca
    11461 cgaaaactcc tacttgagca tgcccaatat tgtttcccaa tattgcccgg atttgtcgtt
    11521 gcagataagc cccccgtgca gcagtgctcc cttcctcgtc aagcacaaaa aaatccccga
    11581 gaagattgac gacgaccgag ggttcgacct atggaagcgc ccccaaacat cgaacccgcg
    11641 ccctgcgacc agcgtgctca gtgagagctc cagcaattgt agcagcgcca gcgacaacct
    11701 gacgagcccc gatgcagcgt ccacgcaatt gtgcctcgcc aacccttcgc atcgccttcc
    11761 ggaagtgacc acgtccaaag cggccagctt caccaacagc acgccctcgt gcttcagcaa
    11821 ggaaagcgcc accaagcaac cgtccctcac caccaccgcc accgccatct cggcccccgg
    11881 atcaacagca ggtctcgccc cacaccatca gcagcagcag cagcatcacc accaccacca
    11941 ccaccaccat caacaccaac aacaccaaca ccaacagcag cagcagcagc agcgccacca
    12001 caacgcagcg cccagcacgc accatcaggc cggccaagcc cctccttcct tccttcaaga
    12061 ttcgcacgcc aagcctgcgg actcgaccaa gctcttcggc agccatgatt cctccaaatc
    12121 cgtctacctc acgagcttcc gcggagacct caacaccaag ttcgttctgc ccgagccgtc
    12181 gaccgggtac agcaacagcg gcaaggcgcc cgcggcagtg tgctaccctt gcttccccga
    12241 tgttccggca gcagacccca aggccggaat tggtcgaggt gcaagaatgg aggtcagtag
    12301 tggaggggat aacctaggcc tcggcggcat tgggttacgg gtgtccccgc cctacaacca
    12361 ccatctctcc tacaactcga ctggcaccac agttcgggac tcgccgcatc tcagcggcac
    12421 cacgaccacc aacaacaatt ccgactctcg tgagcagcat cagcagcagc agcagcaaca
    12481 gcagctcggg ctgtgctcgc aaattctgaa cctggcaggg agtggagttc cgttctccag
    12541 caacggcggc gagcccacgg ctctgagcca cgccgggtcc aacttgctgc acaaggtcgt
    12601 cgatggattc tcctcaatga gcccccaggc gggcaatgtt ggctcgtcgt ccatcaaccc
    12661 gcaattaagc gccgggcgct ccccagtcaa tcctcaatcc agcgtcggct tgctgggcga
    12721 ctcgcagagt agcattgtcc gctcgagatt catgtccaaa ctccccagca agcggagcat
    12781 gcgcgcgcct cggatgcggt ggaccagcac gcttcacgcc cacttcgtcc acgccgtcga
    12841 gcttttggga ggccatgaaa gtaagccagc cctcattcat tgccctcgga tcttgttcga
    12901 accccacagt ttccaattcg gtctcgatcg ttcacttgct gtcgttcgca ctcggtcgtc
    12961 aaactcgctc ggtcaaaggc tgcgatgtgc ttcgaatagc agccgtgaga ccggccgagc
    13021 ttcccttgac cgccatgcca gcccttcgca gtaatgcagc tctttaattc ggtccaattt
    13081 tgtgtcataa tgcacgcagt cagctaggat taggctaggg gtttgcccgt gctttgcctg
    13141 gctcgattta gatgggcgtc tcgaaactgt cagtccgggc cgagattatt gcgcccaatt
    13201 ttccgggcgc atgcgctctt cgtctcagag tcggaaaggt gcttacgagt ggcgcgtgat
    13261 tctcgtcatg gcgtttgcag gagccactcc gaaatccgtc ctggagctga tgaacgtcaa
    13321 ggacctcact cttgcccacg tgaagagcca tttacaggtg agccataact ctccgcccat
    13381 cgcttctaca ttattacatg caatcagact tagacgatga cgactgtgca ctcatgaact
    13441 tagcagctcg gtcgagcagt caatccctct ctcgcttcgt cgcggattag ggcgcgggaa
    13501 atcgtgaaga aatatatacg agcaggacta ggacttcggg gttcgggcta gaggaagcgc
    13561 ccgcagaaat cgtggccgag tcattcatta gagggtttgc ggtaatcacg acgtgacatt
    13621 agaccgatca gctgctgctg ctgtcgctct gttgcgctct ctttcgagcc ggcaggcgca
    13681 gagcggaaag aaatcctagg tagcagcagc agcggggtgt gggaggaagg cccgaagaga
    13741 gctcgtgggg gttgcgttga ggatgaagaa gggttggagt gtagtcgacg agggctcggg
    13801 gtggcctgtg tgggggcgaa tgtgtcgatt taatccgtca atttcttggg gtcagctgtg
    13861 atttggccat ttgggtatac cgtgcaccat cgccgctata gcgccatcgg gcaatgaatg
    13921 actgcgggct gtgatgatca tgcagtcgag ggtcgctaga gtctggttag cttggatgta
    13981 cacgcacacg cgtgcttgca ttatgacgga gactgctgga ggctgacaca gcggccgatt
    14041 ccctcttgca gatgtaccgc acagtcaaaa cgacagacaa gccgagtaat ccctcaggta
    14101 ctgtccctct ccgatcctct ctccactctc ttctttcatc tgctctgccc agccctgcca
    14161 tgctcccttt gcttgaagag cttctgtgtg tatgtgtgtg tacttgagaa cgtggagaga
    14221 tggtgaagag atttgcccgt ccaaagttgc atgctgtgcc gtggggtctc gtcgagggtt
    14281 tgggggtact gctggggtac gacgttgcat atctatccat tctatctctc tctgggggtt
    14341 tctggggacc gactagggca gagactgtgt ctcgtcgtcg tctcgtattc aggacagaat
    14401 tgcggtggca gaatttcgga gctcggatgt cgtgcgtatt cgacatccga atgtccgagt
    14461 gtcggttgcc cagagtgggg accggacggg atgatacgtt cgagttgtgg agaatatgct
    14521 gtacagccct agaggctgga gcagcagtag ggaggggctg gggggagcat gtgagaattc
    14581 tctcgcgtga gatcacagct gccgtagtca aatgcgagcg agcgagccag tacgctgcgt
    14641 cgtacgatga atggctttgc agcatctcgg tgccgaggac acgtctcgcc acagcagcat
    14701 ttagttgcgc tcgcggaggg agtccggaca gtccagacat ggtcaagctc tgaaatgcag
    14761 tgcattacgg ccacttgttg tggtgtaacc tttattacat ctcggacaaa cgacactgaa
    14821 catctatcat tgattgatgc gcatgcgaag aggaccgagg acttttgtcc tctgcctgcc
    14881 cataatttca actgtaagtt agggatgtgt ggtggttgaa tattctccga ttccctgcca
    14941 ttgcatcaca gtcaacggtg tcgaccgcga cgagccgacg atggaagccg aggcatcgtg
    15001 tgctgtcttc gtagaggaaa cgtatgaggt ccatgtaccg tgggatggga ttgggattgt
    15061 cggctttgcc agggcgaggt tgggtgagag ggacgaggcg atgatgggag aaatcgctga
    15121 gagctgagag ctgaaagctg agaggacgat gggggtcagc tttttttttt gggtgctgat
    15181 ggacaaatgt tgccgtgggt gcaggtcaag cagactcgtc gagcgagatc gttacaggag
    15241 ggcggaaagc ggatgacagt gcaacgccga tcgtcgtgga ctcggactcg gggcaggaag
    15301 ggaacacact ctccgagtcg ggtacagacg atgtgcatca ggggggtcgt ttttccctgc
    15361 acatgaaaca atctctgcag gagtacccat caaatttgtg ggccaaccct gacaggtact
    15421 tttcgccgag cttctgacct cccccatctg ctcgcctgtc gaaaggagtg tcgtgtctgt
    15481 cgtcactcct ccatcacaag attccactca cccgtcgcct tgcgccatta aaaattgccc
    15541 cgcccgcgtt ctttgcaagc cagtatctaa ttgtaaccga ccgcttgtgc tctttcccat
    15601 gccatacgaa acgagaattg ctgctccgta aaagtccaag attgtacctc ggccaacagt
    15661 cccagctagc gctccctcat tcacatccct cttcctcctc ctcctcctca atccctccct
    15721 ccctctcccc cctccgtcct cgttgctttc ttcctttcag gccaccagtc gtcgtcgtat
    15781 ccagtcgaag gaccacccga aaggaatcaa atcctcattg aatccccgct cgtagatagc
    15841 aatcaccact cgtaccacta ctagcactac tactattact tctaccactg ttgtcgcctg
    15901 cggatgtttg ctgtagccct agtttgcgtg ggatcgatct catctcagcg cactgaccga
    15961 accgaattga cgtatgcgct gcgtgcagca tgaatcgagg tgcatggtca gcccgagagg
    16021 tggccaaagg cagtcaagga aggcaaggca gcagcggcgc tagcgacgcc gtctcttggc
    16081 agcccaacac tgttccagtg cccaatttca aggtcagtgc tctacttcta ctgctactgc
    16141 tgctactgct gccctccgtc tcgtctcgtt gtccggcgtc ggtccaggtc ccccggtagg
    16201 ggccggatca tgctgtcgta aggggggttc gcttcgctta ggcatgcccg tgtaattgtg
    16261 ctcgtctttc cgatgctcga tttgccccaa gccctgcccc tcccccaccc ccacccccac
    16321 ccccctagct agccccttgc gctgcgtccc gttccgttcc gatccgatcc ggaagccccg
    16381 gcctacccta agctaagcta agctaagccc tctacggggt tgctaagcgg gggctagcgt
    16441 cgtcctcgat ttgtcgcatc cggtggggcc gcgacgagag cttgaaggca gcagcggcga
    16501 acagccccca tcacctcagc atcagcatcg gcatcagcgg actgactgta gactggggga
    16561 gtgcatgtca acgtaatttt cgagggattg aatgtgtggg agctcggtgc cgtactcgag
    16621 aaaagggcga gagggcggag ggagggcggc acttttgtat gattttccag atgcttggga
    16681 gtctcgaggc ctagaatgtg tacagcggac gcttctgggg cgtgtgttga cagtgtgtgt
    16741 gggtttgtgt ggctggcagg caaaagatgg agcgatgggc atgatggagc aagtggcaca
    16801 ttttgagcca agcatggtgg aagatgcggg ttgtaattca atgcaatccc ggaaccagca
    16861 tctacatatg gtcagtgcat cggccctctc gcaactcgtt tcatcgcaga tcacacagcg
    16921 gatcacgaaa atgcccaacc tcgagttcac tctgggcaga cctggctggc acgatcatgc
    16981 ggactcgccc ccgaccgagc tgctcctgct caaatgctga gagctcgaat ttccccgatc
    17041 g
//