| 质粒名称: | pDD56 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 119883 |
| 存储实验室: | Tae Seok Moon |
|
载体骨架基本信息 |
|
| 载体名称: | pAL5000/pMB1 shuttle vector |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 6003bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | pBAD_EYFP |
|
文献引用方法 |
|
| 材料和方法部分: | pDD56 was a gift from Tae Seok Moon (Addgene plasmid # 119883 ; http://n2t.net/addgene:119883 ; RRID:Addgene_119883) |
| 参考文献部分: | Molecular Toolkit for Gene Expression Control and Genome Modification in Rhodococcus opacus PD630. DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS. ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 10.1021/acssynbio.7b00416. Epub 2018 Feb 5. 10.1021/acssynbio.7b00416 PubMed 29366319 |
买家导航
pDD56载体质粒基本信息
pDD56质粒图谱载体图谱和pDD56载体序列质粒序列多克隆位点信息
pDD56质粒载体简介
pDD56质粒序列载体序列
LOCUS pDD56 6003 bp DNA circular SYN 14-APR-2025
DEFINITION Arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin
resistance..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6003)
AUTHORS DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
TITLE Molecular Toolkit for Gene Expression Control and Genome
Modification in Rhodococcus opacus PD630.
JOURNAL ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi:
10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
PUBMED 29366319
REFERENCE 2 (bases 1 to 6003)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1021/acssynbio.7b00416"; journalName: "ACS Synth Biol"; date:
"2018-02-16- 16"; volume: "7"; issue: "2"; pages: "727-738"
COMMENT Sequence Label: pDD56
FEATURES Location/Qualifiers
source 1..6003
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(106..694)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(186..205)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(978..1772)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(1089..1108)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 1699..1718
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
CDS complement(3672..4550)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 4577..4861
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 4784..4803
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
CDS join(4898..4900,4901..4903,4904..5614)
/codon_start=1
/product="enhanced YFP"
/label=EYFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4943..4964)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5204..5223)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5551..5572
/label=EGFP-C
/note="EGFP, forward primer"
terminator 5649..5720
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 5737..5808
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 5809..5829
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 5830..5887
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
primer_bind complement(5911..5928)
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
61 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
301 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
361 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
421 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
481 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
541 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
601 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
661 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
721 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
781 aaggatcttc acctagatcc ttttggggtg ggcgaagaac tccagcatga gatccccgcg
841 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
901 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
961 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
1021 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
1081 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
1141 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
1201 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt gagcctggcg
1261 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
1321 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
1381 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
1441 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
1501 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
1561 gccagccacg atagccgcgc tgcctcgtct tggagttcat tcagggcacc ggacaggtcg
1621 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
1681 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
1741 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
1801 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
1861 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
1921 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
1981 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt
2041 cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc
2101 cctgagtgct tgcggcagcg tgaagctagc gtctaccagg acttttacct gtccgacccg
2161 ttgcaacgga accccccacg gaacccccgc gacacccgct ccccaattgc gttagaacag
2221 cggtggattg tcggcttcgt tgtgggcctt ttgagccgct tcctgttctg ccgcacgctc
2281 tttcctcgcc cgatagccga gtcgcttaac ggtgtccaga tgcagcccga aatgtttggc
2341 cgtttgcggc caagagtggc cctcgtcgtc gtgataggcg cggatgcgtt cgcggcgtgc
2401 agcctgctcg gcgagccact cgctgcgttc ctgcgccacg agccggacga cgtggcgttc
2461 ggatagtccg gtgattcgag cgccttcggc ggcggtcacg cgccgctttt tgcggacagt
2521 cggctgccgg ttgtagccgt cgctgtagcc gtcgctcata gcaatgcctc catggctgac
2581 gcggactttg cgcgccgcgc aactgtgctc gccgccgtgc gcgctgctgc gcccttccgc
2641 gagatggccg actggcgcgc actgagtgtg gcctcgtaga ccacgatccc gtccgcccaa
2701 atgcgcgact tggttgtgat ccaacgccaa atgctgttgg cgatggcgcg gacctcgctg
2761 tccggtagcg gtccgggaca cacgtcgttg cacgggaact cggcgtttcg cgcgtggcac
2821 tcggcataga tcgcgcggcc gagtccgtcc acgttccggg tcggcaggta gatccgcatg
2881 agggcgggac gataggccca caacctgacg gaatcgaaca gtgcgcaatt ccgccctagc
2941 ggcgtcggag ccgctttgta cgtggtctgc tgacgccagc gcggcggtgg catgttcgcg
3001 ccgagctcgg cctcgatgtg gctgagtgtg tagagatctg agtggagcca ttccgtttcc
3061 caggcgatgt ggccggggtt tttggtcatg aggcctgagt aactgcggtc gccgtccacg
3121 gcgcgccgaa ggccttcggc gcacgccgcc atgtatgcga gcggcttacg ccgcgcgtat
3181 tcggtgcgtg gaacaggggc gttgagtgcc cacactgcgt gtgcgtggcc gttggcgcga
3241 ttgcccacga tcgcgttggg cagcggatgg gacccccggg cgctgagcgc tcggagcgct
3301 gcgtctggat ggtctacgtc cacgaccagc aggtttgcca gcgctgttgg gttcgcctcg
3361 atgtaccggc ggcctagggc cgacgcgcgg ctttggcggt agatcccctc gagcagatcg
3421 tcgcttgcca gcggccagta cggcagccag agctgctcaa attcgtcggc gacgtggctc
3481 acgcttggta gtagaccacg attaatcacc ggtgtatggt ccgacacgag ctccaagtca
3541 gatatttcgc tgaggggcca ccccacaact gcacactccc ccgctctccc gtcgagccct
3601 ggtggtggaa caccagcgac agccgagcac ccccaaccac ctgtaccaac cactagttga
3661 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
3721 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
3781 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
3841 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
3901 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
3961 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga
4021 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
4081 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
4141 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc
4201 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
4261 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct
4321 cctggcggga acagtaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc
4381 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
4441 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
4501 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
4561 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
4621 gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg
4681 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
4741 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
4801 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
4861 tacagcggat aaagtagcaa agagaaggag gttaggaatg gtgagcaagg gcgaggagct
4921 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
4981 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
5041 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
5101 cctgcaatgc ttcgcccgct accccgacca catgaagctg cacgacttct tcaagtccgc
5161 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
5221 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
5281 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
5341 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
5401 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
5461 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
5521 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
5581 cgggatcact ctcggcatgg acgagctgta caaggctgcg aatcaaaaat aatactagag
5641 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
5701 gtttgtcggt gaacgctctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
5761 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagtagcgg
5821 ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
5881 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
5941 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
6001 agg
//
