pCAMBIA1301-MpPIF-5xMyc

LOCUS       pCAMBIA1301-MpPIF-5xMyc            14968 bp DNA     circular SYN 03-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..14968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1070..1699
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2128..3201
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3267..3461
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3805..3945
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4131..4719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4806..5600)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6025..6049
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6127..6301
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6341..7366)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(7434..8111)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8302..8323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8338..8368
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8376..8392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8400..8416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            8432..14158
                     /label=Mp3g17350 promoter+gene
     CDS             14174..14203
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             14213..14242
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             14252..14281
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             14291..14320
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             14354..14383
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      14439..14691
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    14713..14737
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc
       61 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat
      121 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc
      181 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg
      241 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc
      301 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg
      361 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg
      421 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg
      481 agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc
      541 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag
      601 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag
      661 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag
      721 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc
      781 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg
      841 ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg
      901 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg
      961 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
     1021 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
     1081 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
     1141 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
     1201 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
     1261 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
     1321 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
     1381 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
     1441 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
     1501 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
     1561 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
     1621 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
     1681 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
     1741 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
     1801 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
     1861 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
     1921 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
     1981 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
     2041 aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac
     2101 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
     2161 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
     2221 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     2281 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     2341 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     2401 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     2461 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     2521 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     2581 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     2641 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     2701 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     2761 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     2821 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     2881 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     2941 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     3001 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     3061 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     3121 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     3181 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     3241 gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
     3301 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
     3361 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
     3421 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     3481 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     3541 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     3601 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     3661 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     3721 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     3781 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     3841 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     3901 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     3961 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4021 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4141 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4201 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4261 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4321 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4381 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4441 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4501 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4561 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4621 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4681 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4741 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
     4801 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     4861 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     4921 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     4981 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     5041 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     5101 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     5161 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     5221 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     5281 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     5341 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     5401 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     5461 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     5521 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     5581 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     5641 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     5701 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     5761 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
     5821 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     5881 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     5941 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     6001 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     6061 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
     6121 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
     6181 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
     6241 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
     6301 actcgagctt gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac
     6361 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     6421 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     6481 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     6541 gatagagttg gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa
     6601 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     6661 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     6721 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     6781 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     6841 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     6901 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     6961 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg
     7021 cgaccggttg tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     7081 gtgcacggcg ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt
     7141 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     7201 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     7261 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     7321 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc
     7381 cgggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
     7441 gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg
     7501 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
     7561 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     7621 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
     7681 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
     7741 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
     7801 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttat
     7861 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     7921 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
     7981 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
     8041 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
     8101 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
     8161 cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct
     8221 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     8281 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     8341 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     8401 aggaaacagc tatgaccatg attacgaatt ctgtaccacg tggagctatc cacatgctgg
     8461 ggcctttctg tgcctgcgct gcgtaccgtt ccctggccag ttcccgggac cctggctttt
     8521 attgctaaac taacctcgat ggatcccgtt gtcggcggga gtggtacgac accctcgccc
     8581 ggtttcggat gatgtttcgt agacgaagag aaatcgcggg ccacggcggc ctgatgttgt
     8641 ggaaccggga cccaggttca tcctggcgat acaaaccgcc acgtggaaga ttctcgtagg
     8701 acgcagccgt cgagttttca ggtgagaaat cggagtgggc aggaggcaaa taccaacttg
     8761 tagctcgtcg aaggccgaaa gcggcccgga taattgcatc gcctctgctg ctctctgtgc
     8821 cgcaccaatg cggcccggat cttaaaatac gaaaaaagaa aaaagaaggc gtcctggggg
     8881 aaggtagtgc caccggtgaa tctcagggat gagctcgaga gctgaattcc cgagcacctg
     8941 gagagagcca tcaatttatt gcgctatggg ggagaaatcc ttgtgccaga gtaggagctc
     9001 gaacgaagtc cgctctcgtt tattaatctc ttacagggtg aggtttaggt gcactgtcct
     9061 gcaggtctgg atggatggta ggatgagagc gaggtgggga ctctggtgat ggagagccct
     9121 ccggagttag gcaaaggcaa gggaaggata ggcaggggag gacgggtcga tgaagcgcgc
     9181 tgccggagta aggtaactct ccgtggaacg ggtgcaggcc aggaccttag gtacaggagt
     9241 gaagagtagc atggctaggc tacgtcgtag gcccgaggag tagagctgag ctaaaagcta
     9301 cactgtacgt acctgtaaag agaagagaag cgagtcgacg gacggaaaga aggaaggaag
     9361 gaaggaaagt ggggcggggc gatgagggag gcgaggagta caagaatgtg gagtagtaag
     9421 gatatcattt tgctggatgt tgctgaagac gatcaagagt cgaattcgag tgcgagtctt
     9481 tggtgctaga gcgggagggt gggcgatccg agtgccagat gtggacgaga gaagcagcgg
     9541 aatcgatggc tgcagattcg gggccagtgg gcagaggcgg ctcgagtctt ccgacgggag
     9601 gattgaggag ggacacgaag agctctgagt gatgtgatgc cattctggta gagaaaaaga
     9661 agggattctg ctcttgggat cgcgcgaggt ccctggggcc agcaagcgcc cagcttgccg
     9721 agcgggctct ctgacgtcga aggtgctgcc gcctctgtgt gtttatccct gtgtctctgt
     9781 gttctaggcg gttgttggcg gagggacgag ggagaggagt ggcgttatgt ctgtctgctt
     9841 gacaagttgg tactccttgt gggtcataag ttgggatttc cttccgcccg tggcactgtt
     9901 tctgtcggcg tgagaagagc aagttttcca gctcaaggtt cagtcctatc gtttttatct
     9961 tcgccttccc tctcccgcgt gtctccatgc atttgatgac cttgtctgtc ggcgacgcta
    10021 gcctcgagca tccatgtcag gcccacgccg ctctagtgtg tatgtttgag tagacagctc
    10081 ttgttctccc acataatgaa atgtccgcga tcactgagcc ttggtaattt tgatctcctt
    10141 tctcgctcac tctgccgcag gagagtgtgc agctacttcg ttaagtgtgc ttgtccgcac
    10201 ggttgacata acctctatga gatctttaga ttctgagcga gatccttctc ttgtgacaga
    10261 gccgtgagaa agagccgggg tggcctgttg tatttcccga ggagatgaat gtacaaactg
    10321 cataaaaact gcatctcctt gatgctccgg aatgatgagg acatgtaagg aagcgttgag
    10381 tacaccgacc cagaagaatc gtaaatgcat ctgcactctg tgttgtagag ttgggcccat
    10441 ttagtcgacg gcgataagta gcagattgac ccgcttagcg gcaagaaccc gttgtgcctc
    10501 tgtttaaact gactgggttc gaattgctcc tacagggact ggatttcgaa tcgggaacgg
    10561 actgaacacc ggaagaatcc tctccggttg tcactacatc cccatcagag gtgagttcag
    10621 tgtgggaagc ctgtcgagag atcggcgagc tggtgattct tacttgcttt ctgtctgtgt
    10681 ttggagcgtc tacctttctc gtaatctacc catacctttc ccgtcactga gaaatgaaga
    10741 ggctttaaaa gtaggtgaac ttcgagactt gggtgccatg agagtagatt agaagtagga
    10801 cgagcaggag acgactgcaa actgtcgaag tcttcacctc tcatcttctc attcccactg
    10861 ccatgcctta ctcacgacca tccactacct ctatgtatgt gggagcgctt ctggcgaagc
    10921 gctgggcatt gacagtgaca ggccgagaag tcgatgccgt agcagtctag gtaggcattt
    10981 gatttacctc ctcctgtttg actcgcagac catgagtcac ctcgttcccg actgggatgg
    11041 taacggtgga gacttagaag ctgttttacc cacaagcgat ttctcactgc cggttaaggg
    11101 aaagtgggac tcattgcccc ataaaaggtc gcacttgtct ggtttggcct tcgtgtaagc
    11161 gcttgctccg gcttttcaat ccccatagtt ttttgtgttc ttgataccat tgtatgtaag
    11221 gtcaatttct tgattgtgga tctttgagaa tgccacttcg tgatgccggc tcgttgacat
    11281 gctgctcttc gtcacatgag cttcgaagag atgttaaagg tcgcgttctt cttcttctac
    11341 aggccaaccc acgatgctgg cgaaactgag tgggcagagg aaagtcatat tgtagctatg
    11401 cagaaccaaa gcgggaaggg caacaaggct atgtggcagc agatgaattt ccagaatggg
    11461 tggtcgagtg tgccagagtc tgaggatgcc gttactcctg tgaagcccat cagctcgtcg
    11521 tctccgggga aggagccggt tcttgtcaat gagcccgcgg tcgacacgca ccactccagt
    11581 gccctcaaca tgcaggaaga cgagatggtg tgctggctgc agtatccgtt ggacgaacac
    11641 ctcgaaagag actactgttc cgagttcttc ggcgagctgc ccaactccaa catatcgaac
    11701 atgcagctga tgaaggattc attcgcaggg caaaatcaag tagcggggcg caacaacagg
    11761 aatccaggtc ccgttaacgt agggagtaac agcagaatgt ctagtgcgga tgcggcgatg
    11821 gccatgggag cggggcgagc tgccggtttg ttgcctcaga caggggtgga ggttttcacc
    11881 aaagttcgga ccactttgca accctacgga ggaaagtggt ccccaaatca gaacaacgcc
    11941 gggaaaacgc tgggagcgat gacctcgacc ctggctccga gcccgtcaag atccccgccg
    12001 atgtctggga acttgtcgac ccccatgctc cccccgaagt ctcagggact cagcttccag
    12061 cctcccagaa cctcaggccc gatgaatttc tcgcatttct ctcgccctgc ggctgtgatg
    12121 aaggccaatc tccagtgcct aggaatgtct cacgtggccc ctccgcacgg gagcgggacg
    12181 aacgtccggc tcaagagcca accctacagc gggaagccac ccgtcgacgc aagcacgtcg
    12241 acggggtcct ccattgcaga gtcaaccacc acgaggcaac gcagcggcgc tttccgcaag
    12301 gataccgaat gccaagtcga aggttccgaa gctcaacaga gtcttggaac cgaatcgagc
    12361 tggccttcga tgcagaattc gctgtcgggc aaggacaccg acgctgtcaa ttcctccgtg
    12421 gatggtgtgg actgcgattc gaagcccggt atagcggatg tggacaattg caggaattct
    12481 agcatcacag gtagtggcgg tttaggaact gaaaagcatc agtctcaagg agcagaagtg
    12541 caggaagctc ccgaacccac cattacctcg tcttctggtg ggtccggtaa tagtgcagat
    12601 agagggaaag aggccgcatc tagtggcaag agaagaacca gggaaggaga ggagtccgag
    12661 tgccagagcg aagtgagtaa cttgttgcaa ccccaaggga gtcaagttgg tgcagtagcc
    12721 cacagggtat gttttatcta gtgatgctaa ctttttcctg aataacatcc cactgactgc
    12781 tctttttatg cgaggatgtg tgagaagtgt acttgcgttt aataaaagcc aatgttgttt
    12841 ctggcagcaa gatggcgagg acgagatggc ccagcaaaag aaacctcaac agagaggctc
    12901 caccactaag aggagcaggg ccgcggaagt ccacaacctt tccgagcgag tgagagtcca
    12961 ccactaactg agaaattgag ctctgcgcca attgtacagg atggtccaag ccgtgagcat
    13021 atgacggcag tggacaatct aggtctcaaa tcttgtttgg gtcgtcaatt ttgtgactga
    13081 tgttagtttt gaagctcttg gcttccgcag ctttactgat agaatttcct gcccatttca
    13141 gaggcgaaga gatcgtatca acgagaagat gaaagctctg caagagctga tccccaattc
    13201 caacaaggtt tgttacagct ttcagccttg ttcttcgtac cttgcctctt cttttggatc
    13261 accgtgcggt aacttgccct tgaaatacag tcaatgatgc agacttttga ttcatattgc
    13321 agacggacaa agcatcgatg ctggatgaag cgatcgagta cttgaagatg ctccagttgc
    13381 aactgcaggt aactatccca gcctttgacc ttcgcagtag ttctctgctc ctccttgcga
    13441 tgcaggtgct gtagagacgc ccgagcacaa atactgaatt caatttgccc tctttagcag
    13501 atgatgtcca tgagaagtgg aatgagcatc ccctctatgg tgatgccggc gggaatgcaa
    13561 caccttcaaa tcccgcaatt gccccaaatg ggcccaatgc ctcccatggg aatgggaatg
    13621 ggcatgggta tgggcatggg cccgatggga atgggaatga gtatgatgga catgggaggc
    13681 ggagctcccg gtcgaccgat gatacccctt ccgtctcaca gtgggccttc tcactctcac
    13741 aacgtatctg caacctcacc agctgtttct atggtagaca ctcatgacca ccgcctgcaa
    13801 aacccgagcg tcatggagtc actgaacgtc ttcatggcgc aacaccggca aatgcaaatg
    13861 gctcctcagg tgatccttct ctttgaatgc cggctggttc tgtccgatct tctgtcagct
    13921 ttgttttttg tgcaaatttc ctaggaggaa ggagcatgtg gttgggagac gatgaagtct
    13981 tgattcttac tcactgtgcc tgttcttgca gccgatgagt atggatgcat tcagtgcatt
    14041 catgattcat cagcaccagc agcaacacca gcagcagcat caacaacatc agctccaaca
    14101 ccaacaccag cattcgctcc atcatcttcc gagcagcatg ggcggtggag ccccccaagg
    14161 atccggtacc atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa
    14221 gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt
    14281 gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg agagcttggg
    14341 cgacctcacc atggagcaaa agctcatttc tgaagaggac ttgaactcgg tataagagct
    14401 cgaatttccc cgatcgggtg accagctcga atttccccga tcgttcaaac atttggcaat
    14461 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
    14521 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
    14581 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
    14641 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattaa
    14701 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
    14761 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
    14821 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
    14881 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
    14941 gtcctaagtt acgcgacagg ctgccgcc
//