| 质粒名称: | pAAV-CAG-DIO-mBaoJin-LaminA-WPRE-polyA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000669 |
| 产品用途: | AAV, expresses mBaoJin-LaminA in cells |
| 存储实验室: | Hu Zhao Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl |
|
插入基因信息 |
|
| 插入基因名称: | mBaoJin-LaminA |
| 物种来源: | Obelia medusa, H.sapiens |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Leslov G.D., Subach O.M., Zhang W., Kuzmicheva T.P., Vlaskina A.V., Samygina V.R., Chen L., Ye X., Nikolaeva A.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S., Drobizhev M., Mishin A.S., Piatkevich K.D.
Nat. Methods 2024 214 21, 657–665 (2024)
|
买家导航
pAAV-CAG-DIO-mBaoJin-LaminA-WPRE-polyA载体质粒基本信息
pAAV-CAG-DIO-mBaoJin-LaminA-WPRE-polyA质粒图谱载体图谱和pAAV-CAG-DIO-mBaoJin-LaminA-WPRE-polyA载体序列质粒序列多克隆位点信息
pAAV-CAG-DIO-mBaoJin-LaminA-WPRE-polyA质粒载体简介
pAAV-CAG-DIO-mBaoJin-LaminA-WPRE-polyA质粒序列载体序列
LOCUS pAAV-CAG-DIO-mBaoJin-LaminA-WPRE-polyA 7745 bp DNA circular SYN 16-JAN-2025
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7745)
AUTHORS Rui Lin
TITLE Direct Submission
JOURNAL Exported Apr 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7745
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature complement(3..11)
/label=glycine-serine-glycine linker
rep_origin complement(126..793)
/direction=LEFT
/label=pUC ori
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication;
arrowindicates direction of (+) strand synthesis"
misc_feature 2458..2647
/label=ITR Addgene
misc_feature 2648..2653
/label=5' splice site
misc_feature 2651..3587
/label=CAG
enhancer 2774..2899
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2901..3178
/label=chicken beta-actin promoter
primer_bind 3579..3600
/label=0711-PM960-F
misc_feature 3624..3657
/label=lox2722
misc_feature 3708..3741
/label=loxP
misc_feature complement(3750..5741)
/label=LaminA
primer_bind 4614..4625
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 5756..5783
/label=LaminA-mScarlet3H-R
CDS complement(5784..6485)
/codon_start=1
/label=mBaoJin
/translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
EAHLKGMDELYK"
primer_bind 5784..5805
/label=0711-PM960-R
primer_bind 5811..5835
/label=0714-StayBlue-R
primer_bind complement(6425..6459)
/label=0714-StayBlue-F
primer_bind complement(6464..6492)
/label=0711-PM960-F
regulatory 6482..6491
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 6505..6538
/label=lox2722
misc_feature 6589..6622
/label=loxP
misc_feature 6647..7235
/label=WPRE
/note="woodchuck hepatitis virus
posttranscriptionalregulatory element"
CDS complement(7118..7129)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 7278..7399
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 7502..7691
/label=ITR Addgene
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
2701 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
2761 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
2821 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
2881 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
2941 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
3001 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
3061 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
3121 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
3181 agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
3241 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
3301 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
3361 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg
3421 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
3481 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
3541 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgga tccggtaccg
3601 gatcctctag agtcgactcc ggaataactt cgtataggat actttatacg aagttatgca
3661 gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata acttcgtata
3721 gcatacatta tacgaagtta tggcgcgcct tacatgatgc tgcagttctg ggggctctgg
3781 gttcgggggc tggagttgcc caggaggtag gagcgggtga ccagattgtc cccgaagctg
3841 ccacccccac tgccccccac actgcggtag ctgcgagtga ccgtgacact ggaggcagaa
3901 gagccagagg agatgggtcc gcccacctgg gctcctgagc cgctggcaga tgccttgtcg
3961 gcaggctgcc cgcaggtccc gcacagcacg gtgcgcgagc gcaggttgta ctcagcgggg
4021 tcccccgagc tgctgcagtg ggagccgtgg tggtgatgga gcaggtcatc tccatcctca
4081 tcctcgtcgt cctcaaccac agtcactgag cgcaccagct tgcgcatggc cacttcttcc
4141 ccagtggagt tgatgagagc cgtacgcagg ctgttcccgc agccccaggt gttctgtgcc
4201 ttccacacca ggtcggtagg ggggctgtgg gtggccccag ctcctgcagc ccagatcgtc
4261 accacctgcc cagccttcag ggtgaacttt ggtgggaacc ggtaagtcag caagggatca
4321 tctccattct ggcgcttgat ctgccaattg cccatggact ggtcctcatt ggacttgttg
4381 cgcagccgga caaacttgcc ctcctcgtcc acctcctcca cggccacgcg cccgctagtg
4441 cgtgcgtgct gtgagaagct gctgcggctc tcagtggact ccagtttgcg ctttttggtg
4501 acgctgcccc caccctgtgt ctgggatgag tgagaggaag cacggccacg gctgcgctgc
4561 gaggtagggc tgggggacag gcgtagcctc tcctcctcgc cctccaagag cttgcggtag
4621 gcgtggatct ccatgtccag ggccagcttg atgtccagaa gctcctggta ctcgtccagc
4681 tgctgctgca tccttgcccg catctcggcc atctcccgct ccttttccgc cagcagccgc
4741 cggctggtgt cccgctcacg ggccagtgag tcctccaggt ctcgaagctt tgcctccttg
4801 gctgccagct gcttctggag ctggctgagc tgggcagaga ggctgtcgat gcggatgcgc
4861 gactgctgca gctcctcgtg ggcagccccc accaggttgc tgttcctctc agcagactgc
4921 ctggcattgt ccagcttggc agaataagtc ttctccagct ccttcttata ctgctccacc
4981 tggtcctcat gctgggcccg cagttcctgc agcgcatccg ccagccggct ctcaaactca
5041 cgctgcttcc cattgtcaat ctccaccagt cgggtctcat gacggcgctt ggtctcacgc
5101 agctcctcac tgtagatgtt cttctggaag tccagttcct ccttcatggt ctgcagcctg
5161 ttctcagcat ccacccgccg cagcatctca tcctgaagtt gcttcttggc ctcacctagg
5221 gctgcctcaa gcttggccac ctggccccgc agatcatgca gctcgccctc cagcgtgcgc
5281 ttctcactga gagcagtgct cagtgcggcc tccttggagt tcagcagagc ctccaggtcc
5341 ttcagccgag cctgagcagc tatcaggtca ccctccttct tggtattgcg cgctttcagc
5401 tccttaaact cctcacgcac tttgctcagc tccagctgca ggcgggcgcg ctccttggct
5461 actgagtcaa gggtcttgcg ggcatccccg agctcggcct cgtaggcggc cttgatgccg
5521 gacacctcgc ggctgaccac ctcttcagac tcggtgatgc gaaggcgcag ccctgcgttc
5581 tccgtttcca gcgagcgcac acggtcgatg tagaccgcca agcgatcatt gagctcctgc
5641 aggtcctcct tctcctgcag ccgggtgatg cgggtgggcg acagcggagt ggagctggcc
5701 tgcgccccgc tgcgggtggc gcgccgctgg gacggggtct ccatgaattc gaagcttgag
5761 ctcgagatct gagtccggcc ggacttatac agctcgtcca tgccctttaa gtgtgcttcg
5821 agagtctctg attgacagat atgatcgcgt tcctcggcat catctttgct ttgtgtgtat
5881 tgtttacgaa tccagtgata tgggacatca ggtgctggtt gattatgaag aggcttgcag
5941 attgtatatt ggtgagcctc gacgtatttt gatttatccg ataataaagg ataaagcaag
6001 gtcactgggc attctactcc atcatctctg ggtatttgtg ggacttcatt cggtaatgag
6061 acattcatat ctccagttaa gactggacta ttttctttaa aatcctgacc ggtaaattct
6121 acaatgttgt gataagtccc atttttcata aagtgttggt gttttgcatg gatactccca
6181 tcgcctttat attgaatatg acgatcgtag gtaaagcctc cgggcattac ttcacgaaac
6241 cagttcttca gtccactagg atatttggta taatatttca ttccataacc aaaggttgtc
6301 ccaagtgctg cccatgacat cggtagtttt ccacttgtac aaacatattt tcctttatga
6361 gaaccttcat gtgagttccc ttcaccttcg ccttcaacgg taaacgattt gccattgatg
6421 gttcctttaa gttgaaattt aaatggtgta ctagccatgt tttcctcctc tccctttgag
6481 accatggtgg cgaccggtgc tagcataact tcgtataaag tatcctatac gaagttattt
6541 gccttaaccc agaaattatc actgttattc tttagaatgg tgcaaagaat aacttcgtat
6601 aatgtatgct atacgaagtt atgaattcga tatcaagctt atcgataatc aacctctgga
6661 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
6721 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
6781 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
6841 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
6901 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
6961 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
7021 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
7081 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
7141 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
7201 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ccgggcggcc
7261 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
7321 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
7381 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
7441 ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
7501 ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca
7561 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
7621 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
7681 gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
7741 gcgct
//
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