| 质粒名称: | pAAVLINK-3'-DOCK8-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001706 |
| 产品用途: | 3' AAVLINK plasmid for DOCK8 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DOCK8 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-DOCK8-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-DOCK8-FLAG载体质粒基本信息
pAAVLINK-3'-DOCK8-FLAG质粒图谱载体图谱和pAAVLINK-3'-DOCK8-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-DOCK8-FLAG质粒载体简介
pAAVLINK-3'-DOCK8-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-DOCK8-FLAG 6708 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6708)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6708
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3447
/codon_start=1
/label=DOCK8
/translation="ENEQAEKMNISLAFFLYDLLSLMDRGFVFNLIRHYCSQLSAKLSN
LPTLISMRLEFLRILCSHEHYLNLNLFFMNADTAPTSPCPSISSQNSSSCSSFQDQKIA
SMFDLTSEYRQQHFLTGLLFTELAAALDAEGEGISKVQRKAVSAIHSLLSSHDLDPRCV
KPEVKVKIAALYLPLVGIILDALPQLCDFTVADTRRYRTSGSDEEQEGAGAINQNVALA
IAGNNFNLKTSGIVLSSLPYKQYNMLNADTTRNLMICFLWIMKNADQSLIRKWIADLPS
TQLNRILDLLFICVLCFEYKGKQSSDKVSTQVLQKSRDVKARLEEALLRGEGARGEMMR
RRAPGNDRFPGLNENLRWKKEQTHWRQANEKLDKTKAELDQEALISGNLATEAHLIILD
MQENIIQASSALDCKDSLLGGVLRVLVNSLNCDQSTTYLTHCFATLRALIAKFGDLLFE
EEVEQCFDLCHQVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMSLAS
LVGRAPDFNEEHLRRSLRTILAYSEEDTAMQMTPFPTQVEELLCNLNSILYDTVKMREF
QEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCYTEAAMCLVHAAALVAEY
LSMLEDHSYLPVGSVSFQNISSNVLEESVVSEDTLSPDEDGVCAGQYFTESGLVGLLEQ
AAELFSTGGLYETVNEVYKLVIPILEAHREFRKLTLTHSKLQRAFDSIVNKDHKRMFGT
YFRVGFFGSKFGDLDEQEFVYKEPAITKLPEISHRLEAFYGQCFGAEFVEVIKDSTPVD
KTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHE
QYRRNTVLTTMHAFPYIKTRISVIQKEEFVLTPIEVAIEDMKKKTLQLAVAINQEPPDA
KMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVE
KNKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKPIFRVESQKRDSFHRSSF
RKCETQLSQGS"
misc_feature 1646..1651
/label=SDS
misc_feature 1743..1748
/label=SDS
misc_feature 2340..2345
/label=SDS
misc_feature 2960..2965
/label=SDS
CDS 3454..3519
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3523..3954
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3667..3672
/label=SDS
repeat_region 3971..4111
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3971..4100
/label=AAV2 ITR
rep_origin 4186..4641
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4923..5027
/gene="bla"
/label=AmpR promoter
CDS 5028..5888
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6059..6647
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gaaaatgaac aggcggaaaa gatgaacatc
301 agcctggctt tcttcttgta tgaccttctc tccctcatgg atcggggctt tgtgtttaac
361 ctcatcagac attattgcag ccagctgtca gccaagctca gtaaccttcc aacgctcatt
421 tccatgaggc tagagttcct gagaatcctc tgtagccatg agcattacct caatctgaac
481 ctttttttta tgaatgctga tactgctcca acatctcctt gtccttccat atcttcccag
541 aactcaagct cctgctccag cttccaggac cagaagatcg ccagcatgtt cgatctgact
601 tccgagtacc gccagcagca cttcctcacc gggctcctct tcacagaact ggctgctgcc
661 ctggatgccg aaggggaagg aatcagcaaa gtacaaagga aagctgtcag tgcaattcac
721 agcctgctaa gttctcacga cctggaccca cgctgtgtca aaccagaggt gaaggtcaaa
781 atcgccgccc tttacctacc tttagttggc atcattttgg atgctttgcc acagctctgt
841 gactttacag ttgcagatac tcgcagatac cgcaccagtg gctcggatga agaacaagaa
901 ggagccggtg ccattaacca gaatgtggct ctggccatag cagggaataa tttcaatttg
961 aaaacaagtg gaatagtgct gtcttccttg ccctataagc agtacaacat gctgaacgcg
1021 gacactactc gcaacctcat gatctgcttc ctctggatca tgaaaaatgc tgatcagagc
1081 ctcattagga agtggattgc tgacctgcca tcaacgcagc tcaacaggat tttagatcta
1141 cttttcatct gtgtgttatg ttttgagtat aagggaaaac agagttctga caaagtcagt
1201 acccaagtcc tgcagaagtc aagggatgtc aaggcccggc tggaagaggc tttgctgcgt
1261 ggggaagggg ccagagggga gatgatgcgc cgccgggctc cagggaacga ccgatttcca
1321 ggcctaaatg aaaatttgag atggaagaaa gagcagacac attggcggca agctaatgag
1381 aagctagata aaacaaaggc cgagttagat caagaagcct tgatcagtgg caatctggct
1441 acagaagcac atttaatcat cctggatatg caggaaaaca ttatccaggc gagctcggct
1501 ctggactgta aagacagcct gctgggaggt gttctgaggg tgctggtgaa ttctctgaac
1561 tgtgatcaga gtaccaccta cctgactcac tgctttgcaa cactccgtgc tctcatcgcc
1621 aagtttggag acttactctt cgaagaggag gtggaacagt gtttcgacct atgtcaccaa
1681 gtcctgcacc actgcagcag cagcatggat gtcacccgga gccaagcctg tgccaccctt
1741 tacctcctca tgaggttcag ttttggagcc accagtaatt ttgcaagagt aaagatgcaa
1801 gtaaccatgt ccctggcatc tttggtggga agagcaccag actttaatga agagcacctg
1861 agaagatcct tgaggacaat tttggcctat tcagaagagg acacagccat gcagatgact
1921 ccttttccca cccaggtgga ggaacttctc tgtaatctga atagcatctt atatgacaca
1981 gtgaaaatga gggaatttca ggaagatcct gagatgctta tggatctcat gtacagaatt
2041 gccaagagtt accaggcatc tcctgatctg cggctgacct ggctccagaa catggcagag
2101 aaacacacca agaagaagtg ctacacggag gctgccatgt gcctggtgca cgccgctgcg
2161 ttagtggctg agtatctgag catgctggag gaccacagct acctgcccgt gggcagtgtc
2221 agcttccaga atatttcttc caatgtgctg gaggagtctg tggtctctga ggacaccctg
2281 tcacctgacg aggatggggt gtgcgcaggc cagtacttca ccgagagtgg cctggtaggc
2341 ctcctggagc aggccgcgga gctcttcagc acgggaggct tatatgagac agttaatgag
2401 gtctacaagc tggtcatccc catcctagaa gcgcatcgag aattccggaa gctgacactc
2461 actcacagca agctgcagag agccttcgac agcatcgtta acaaggatca taagagaatg
2521 tttggaacct acttccgagt tggtttcttt ggatccaaat ttggggattt ggatgaacag
2581 gaatttgtct acaaagagcc tgcaattacc aagcttcctg agatctcaca tagactagag
2641 gcattttatg gtcaatgttt tggtgcagaa tttgtggaag tgattaaaga ctccactcct
2701 gtggacaaaa ccaagttgga tcctaacaag gcctacatac agatcacttt tgtggagccc
2761 tactttgatg agtatgagat gaaagacagg gtcacatact ttgagaagaa tttcaacctc
2821 cggaggttca tgtacaccac cccgttcacc ctggaggggc ggcctcgggg agagctgcat
2881 gagcagtaca gaaggaacac agtcctgacc actatgcacg ccttccccta catcaagacc
2941 aggatcagcg tcatccagaa ggaggagttt gttttgacac cgattgaagt tgccattgaa
3001 gacatgaaga agaagaccct gcagttagca gttgccatta accaggagcc gcctgatgca
3061 aagatgcttc agatggtgct gcaaggctct gtgggagcta ctgtaaatca gggaccactg
3121 gaagtagccc aagtgttttt ggctgaaatt cctgctgatc caaaactcta tcgacatcac
3181 aacaagttga ggttatgctt taaggaattc atcatgagat gtggtgaagc tgtagagaaa
3241 aacaagcgtc tcatcacggc agaccagagg gaatatcagc aggaactcaa aaagaactat
3301 aacaagctaa aagagaacct caggccaatg atcgagcgga aaattccaga actgtacaag
3361 ccaatattca gagttgagag tcaaaagagg gactccttcc acagatctag tttcaggaaa
3421 tgtgaaaccc agttgtcaca gggcagctgt acagactaca aagaccatga cggtgattat
3481 aaagatcatg acatcgacta caaggatgac gatgacaagt aactcgagat aatcaacctc
3541 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
3601 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
3661 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
3721 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta
3781 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt
3841 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
3901 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaacggacc
3961 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
4021 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
4081 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
4141 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
4201 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
4261 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
4321 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
4381 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
4441 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
4501 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
4561 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
4621 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
4681 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
4741 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
4801 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
4861 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
4921 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
4981 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
5041 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
5101 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
5161 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
5221 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
5281 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
5341 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
5401 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
5461 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
5521 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
5581 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
5641 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
5701 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
5761 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
5821 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
5881 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
5941 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
6001 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
6061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
6121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
6181 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
6241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
6301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
6361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
6421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
6481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
6541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
6601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
6661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
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pAAVLINK-3'-DOCK8-FLAG质粒载体应用举例
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