pSPYCE35S-bk1-YFP-C-bk2

LOCUS       pSPYCE35S-bk1-YFP-C-bk2             4158 bp DNA     circular SYN 19-MAR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Youbio
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..42
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        566..911
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     852..871
                     /label=YNEF
     promoter        920..922
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             998..1249
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=YFP-C
                     /note="mammalian codon-optimized"
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1256..1276
                     /label=同源臂
     misc_feature    1289..1309
                     /label=同源臂
     terminator      1323..1575
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1584..1600)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2074..2178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2179..3039
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3210..3798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
       61 gcatgcctgc aggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
      121 tggttagaga ggcttacgca gcaggtctca tcaagacgat ctacccgagc aataatctcc
      181 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      241 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      301 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
      361 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
      421 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
      481 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      541 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      601 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      661 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      721 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      781 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      841 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      901 tggagagaac acgggggacc aggtcgactc tcgaggatcc ggtacccggt atcgatttaa
      961 aaggcctatg tacccatacg atgttccaga ttacgctgac aagcagaaga acggcatcaa
     1021 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1081 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1141 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1201 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga ggcctcaggt
     1261 cgactctcga ggatcaacta gtgagctcag tacccggtat cgatttaaat aagaatttcc
     1321 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     1381 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     1441 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     1501 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     1561 ctatgttact agatcgggaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     1621 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     1681 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     1741 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     1801 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     1861 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     1921 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     1981 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     2041 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2101 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2161 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2221 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2281 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     2341 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     2401 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     2461 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     2521 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     2581 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2641 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2701 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     2761 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     2821 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     2881 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     2941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3001 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3061 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3121 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3841 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3901 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3961 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4021 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4081 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     4141 gctcgtatgt tgtgtgga
//