| 质粒名称: | pQL83 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002510 |
| 产品用途: | Synthetic ETS*-driven E1A expression vector ((GGAA)21-PhCMVmin-E1A-pA) |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | Synthetic |
| 拷贝数: | 中拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | E1A |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
pQL83
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pQL83载体质粒基本信息
pQL83质粒图谱载体图谱和pQL83载体序列质粒序列多克隆位点信息
pQL83质粒载体简介
pQL83质粒序列载体序列
LOCUS pQL83 5923 bp DNA circular SYN 16-MAY-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5923)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5923
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 218..237
/label=QL143-F
primer_bind 220..241
/label=QL214 83-F
misc_feature 238..265
/label=GGAA
misc_feature 266..293
/label=GGAA
misc_feature 294..321
/label=GGAA
misc_feature 328..475
/label=hcmvmin
misc_feature 397..411
/label=New Feature
primer_bind complement(460..475)
/label=oYW11
primer_bind 467..506
/label=QL111 E1A-F
primer_bind 486..506
/label=E1-F
CDS join(487..1039,1156..1472)
/codon_start=1
/product="E1A"
/label=E1A
/note="other spliced forms exist with larger introns"
/protein_id="DAA00639.1"
/translation="MRHIICHGGVITEEMAASLLDQLIEEVLADNLPPPSHFEPPTLHE
LYDLDVTAPEDPNEEAVSQIFPDSVMLAVQEGIDLLTFPPAPGSPEPPHLSRQPEQPEQ
RALGPVSMPNLVPEVIDLTCHEAGFPPSDDEDEEGEEFVLDYVEHPGHGCRSCHYHRRN
TGDPDIMCSLCYMRTCGMFVYSPVSEPEPEPEPEPEPARPTRRPKMAPAILRRPTSPVS
RECNSSTDSCDSGPSNTPPEIHPVVPLCPIKPVAVRVGGRRQAVECIEDLLNEPGQPLD
LSCKRPRP"
primer_bind 487..506
/label=QL43
primer_bind complement(1452..1473)
/label=QL152-R
primer_bind complement(1453..1493)
/label=QL112 E1A-R
primer_bind complement(1453..1485)
/label=QL44
primer_bind complement(1454..1481)
/label=E1-R
primer_bind 1475..1525
/label=QL217 F
terminator 1520..1747
/label=bGH PA
primer_bind complement(1728..1747)
/label=QL170-R
primer_bind complement(1757..1779)
/label=QL215 83-R
misc_feature 2415..2492
/label=SV40 ORI
CDS 2634..3425
/label=Kan/neoR
primer_bind complement(3765..3785)
/label=M13-rev
misc_binding complement(3791..3813)
/label=LacO
promoter complement(3818..3847)
/label=lac
CDS complement(4930..5589)
/label=AmpR
promoter complement(5829..5857)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgtacgga
241 aggaaggaag gaaggaagga aggaaggaag gaaggaagga aggaaggaag gaaggaagga
301 aggaaggaag gaaggaagga agctagctcg agctcggtac ccgggtcgag taggcgtgta
361 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
421 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc
481 gaattcatga gacatattat ctgccacgga ggtgttatta ccgaagaaat ggccgccagt
541 cttttggacc agctgatcga agaggtactg gctgataatc ttccacctcc tagccatttt
601 gaaccaccta cccttcacga actgtatgat ttagacgtga cggcccccga agatcccaac
661 gaggaggcgg tttcgcagat ttttcccgac tctgtaatgt tggcggtgca ggaagggatt
721 gacttactca cttttccgcc ggcgcccggt tctccggagc cgcctcacct ttcccggcag
781 cccgagcagc cggagcagag agccttgggt ccggtttcta tgccaaacct tgtaccggag
841 gtgatcgatc ttacctgcca cgaggctggc tttccaccca gtgacgacga ggatgaagag
901 ggtgaggagt ttgtgttaga ttatgtggag caccccgggc acggttgcag gtcttgtcat
961 tatcaccgga ggaatacggg ggacccagat attatgtgtt cgctttgcta tatgaggacc
1021 tgtggcatgt ttgtctacag taagtgaaaa ttatgggcag tgggtgatag agtggtgggt
1081 ttggtgtggt aatttttttt ttaattttta cagttttgtg gtttaaagaa ttttgtattg
1141 tgattttttt aaaaggtcct gtgtctgaac ctgagcctga gcccgagcca gaaccggagc
1201 ctgcaagacc tacccgccgt cctaaaatgg cgcctgctat cctgagacgc ccgacatcac
1261 ctgtgtctag agaatgcaat agtagtacgg atagctgtga ctccggtcct tctaacacac
1321 ctcctgagat acacccggtg gtcccgctgt gccccattaa accagttgcc gtgagagttg
1381 gtgggcgtcg ccaggctgtg gaatgtatcg aggacttgct taacgagcct gggcaacctt
1441 tggacttgag ctgtaaacgc cccaggccat aagcggccgc tcgagtctag agggcccgtt
1501 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
1561 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
1621 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
1681 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
1741 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
1801 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
1861 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
1921 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
1981 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
2041 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
2101 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
2161 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
2221 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
2281 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
2341 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
2401 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
2461 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
2521 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
2581 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
2641 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
2701 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
2761 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
2821 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
2881 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
2941 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
3001 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
3061 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
3121 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
3181 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
3241 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
3301 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
3361 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
3421 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
3481 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
3541 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
3601 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
3661 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
3721 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
3781 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
3841 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
3901 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
3961 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
4021 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
4081 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
4141 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
4201 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
4261 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
4321 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
4381 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
4441 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
4501 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
4561 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
4621 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
4681 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
4741 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
4801 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
4861 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
4921 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
4981 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
5041 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
5101 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
5161 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
5221 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
5281 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
5341 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
5401 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
5461 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
5521 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
5581 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
5641 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
5701 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
5761 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
5821 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
5881 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
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