| 质粒名称: | pYK671 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000837 |
| 产品用途: | Mammalian PhCMV-driven constitutive MCP-GNCR expression vector (PhCMV-MCP-GNCR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | MCP-GNCR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK671
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK671载体质粒基本信息
pYK671质粒图谱载体图谱和pYK671载体序列质粒序列多克隆位点信息
pYK671质粒载体简介
pYK671质粒序列载体序列
LOCUS pYK671 6552 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6552)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6552
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..951
/label=oYK57-NLS-F
primer_bind 896..942
/label=oYK05-mcp-F
primer_bind 910..933
/label=Oscl32-1
primer_bind 910..933
/label=Oscl42-1
primer_bind 917..933
/label=oSCL1118
regulatory 917..925
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 925..1311
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
primer_bind 925..951
/label=oYK57-mcp-mCherry-F
primer_bind 925..951
/label=oYK228_mcp_F
primer_bind complement(1291..1341)
/label=oSCL093
primer_bind complement(1291..1311)
/label=Oscl32-2
primer_bind 1311..1331
/label=oLZ048 NSP3-F
misc_feature 1312..1346
/label=linker
primer_bind 1312..1341
/label=oSCL388
primer_bind 1312..1331
/label=oSCL1192
primer_bind 1312..1329
/label=oLZ147 NS3a F
primer_bind 1312..1328
/label=oYF0033
primer_bind 1312..1327
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1322..1347)
/label=oSCL1119
primer_bind complement(1322..1341)
/label=oSCL075
primer_bind 1326..1372
/label=oLZ146 GNCR1-N F
primer_bind 1328..1371
/label=oYK671_GNCR_F
primer_bind complement(1329..1347)
/label=oYK460_MCPGS_R
primer_bind complement(1330..1348)
/label=oYK05-mcp-R
primer_bind complement(1330..1348)
/label=oYK82_mcp_R
primer_bind 1347..1381
/label=oYF0011
CDS 1348..2046
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 1348..1381
/label=oYF005
primer_bind 1348..1375
/label=oYF0019
primer_bind complement(2023..2072)
/label=Primer 1
primer_bind complement(2023..2072)
/label=oYK500_GNCR_R
primer_bind complement(2025..2046)
/label=oYF006
primer_bind complement(2025..2046)
/label=oYF0012
primer_bind complement(2025..2046)
/label=oYF0020
primer_bind complement(2050..2072)
/label=oYK57-MCP-mCherry-R
terminator 2146..2373
/label=bGH PA
misc_feature 2419..2847
/label=f1 origin
misc_feature 2852..3195
/label=SV40 early promoter and origin
misc_feature 3041..3118
/label=SV40 ORI
CDS 3260..4051
/label=Kan/neoR
misc_feature 4225..4355
/label=SV40 early polyadenylation signal
primer_bind complement(4391..4411)
/label=M13-rev
misc_binding complement(4417..4439)
/label=LacO
promoter complement(4444..4473)
/label=lac
misc_feature complement(4738..5411)
/label=pUC origin
CDS complement(5559..6218)
/label=AmpR
promoter complement(6458..6486)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcggctagcg
901 tttaaactta agcttgccac catggcttca aactttactc agttcgtgct cgtggacaat
961 ggtgggacag gggatgtgac agtggctcct tctaatttcg ctaatggggt ggcagagtgg
1021 atcagctcca actcacggag ccaggcctac aaggtgacat gcagcgtcag gcagtctagt
1081 gcccagaaga gaaagtatac catcaaggtg gaggtcccca aagtggctac ccagacagtg
1141 ggcggagtcg aactgcctgt cgccgcttgg aggtcctacc tgaacatgga gctcactatc
1201 ccaattttcg ctaccaattc tgactgtgaa ctcatcgtga aggcaatgca ggggctcctc
1261 aaagacggta atcctatccc ttccgccatc gccgctaact caggtatcta cgccagcgga
1321 agtggcggag gaggcgacgt cggatccgac atcgagaagc tgtgcaagaa ggcggaagag
1381 gaggccaagg aggcccagga gaaggccgac gagctgagac aacgacaccc cgattcgcag
1441 gcggcggaag acgcggagga tctcgcaaat ctggctgtcg ccgccgtgct gaccgcttgt
1501 ctgctggccc aggagcatcc caacgccgat atcgctaagc tgtgcatcaa ggccgcgagc
1561 gaagctgcgg aggccgcgtc aaaagccgcc gagctggccc aacggcaccc agacagtcaa
1621 gcagccagag atgctatcaa actcgcctct caagccgccc gcgccgtgat cctggccatc
1681 atgctcgccg cagagaatcc caacgctgat attgcaaagc tatgtattaa ggcggccagc
1741 gaagccgcag aggctgcctc gaaagctgcg gaacttgcac agcggcaccc cgactcccag
1801 gccgcccgag atgcaattaa actggccagt caggccgctg aggcggtgga gagagccatc
1861 tggctggccg ccgagaaccc caatgccgat atcgccaaaa aatgcatcaa agcggcctcc
1921 gaagcagccg aggaggcctc taaagctgcc gaagaggctc aaagacaccc agacagccag
1981 aaggcacgcg acgagatcaa ggaggcctct cagaaggccg aggaggtgaa ggagagatgc
2041 aagagctaag gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc
2101 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
2161 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
2221 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
2281 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
2341 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
2401 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
2461 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
2521 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
2581 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
2641 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
2701 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
2761 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
2821 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
2881 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
2941 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
3001 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
3061 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
3121 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
3181 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
3241 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
3301 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
3361 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
3421 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
3481 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
3541 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
3601 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
3661 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
3721 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
3781 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
3841 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
3901 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
3961 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
4021 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
4081 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
4141 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
4201 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
4261 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
4321 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
4381 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
4441 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
4501 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
4561 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
4621 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
4681 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
4741 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
4801 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
4861 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
4921 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
4981 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
5041 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
5101 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
5161 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
5221 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
5281 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
5341 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
5401 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
5461 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
5521 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
5581 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
5641 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
5701 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
5761 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
5821 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
5881 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
5941 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
6001 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
6061 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
6121 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
6181 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
6241 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
6301 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
6361 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
6421 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
6481 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
6541 ccacctgacg tc
//
pYK671载体图谱质粒图谱pdf版和pYK671载体序列质粒序列等相关资料下载
pYK671质粒载体应用举例
- 上一篇：pYK664
- 下一篇：没有了
