RaMorideER

LOCUS       RaMorideER             8855 bp DNA     circular SYN 17-NOV-2024
DEFINITION  3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin.
ACCESSION   .
VERSION     .
KEYWORDS    pLJM1-RaMorideER
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
FEATURES             Location/Qualifiers
     source          1..8855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        290..493
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     444..464
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             582..1298
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSQNVYLMADKQKNGIK
                     SNFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQVKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1329..1346
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1359..2030
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="DNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAKL
                     QVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGVV
                     EVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISHA
                     LKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHSG
                     SQ"
     misc_feature    2097..2214
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2263..2773
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2692..2710
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2795..3394
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2795..2814)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3291..3311
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3522..3755
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3827..3948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3864..3883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3918..3937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3988..4123
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4050..4069
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4073..4084)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4143..4162)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4144..4162)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4172..4189)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4172..4188)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4181..4203)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4330..4785
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4417..4436)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4627..4648
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4811..4915
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4916..5776
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5134..5153)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5947..6535
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6436..6455
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6689..6706
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6823..6844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6859..6889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6897..6913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6902..6924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6921..6937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6921..6937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6956..6976
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6958..6976
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7004..7230
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7231..7411
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7458..7583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8076..8309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8494..8538
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     join(8836..8855,1)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
       61 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      121 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      181 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      241 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      301 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      361 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      421 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      481 tataagcaga gctggtttag tgaaccgtca gatccgctag catgctgcta tccgtgccgt
      541 tgctgctcgg cctcctcggc ctggccgtcg ccgaccggtc gatggtgagc aagggcgagg
      601 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      661 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
      721 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
      781 acggcctgaa gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      841 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      901 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      961 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1021 acagccagaa cgtctatctg atggccgaca agcagaagaa cggcatcaag agcaacttca
     1081 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
     1141 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccaggtga
     1201 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1261 ccgccgggat cactctcggc atggacgagc tgtacaagcg cggatccgcg tctggtggtg
     1321 gtggtggtct agttccacgt ggatctgcct caggagcaga caacaccgag gacgtcatca
     1381 aggagttcat gcagttcaag gtgcgcatgg agggctccgt gaacggccac tacttcgaga
     1441 tcgagggcga gggcgagggc aagccctacg agggcaccca gaccgccaag ctgcaggtga
     1501 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct
     1561 ccaaggccta cgtgaagcac cccgccgaca tccccgacta catgaagctg tccttccccg
     1621 agggcttcac ctgggagcgc tccatgaact tcgaggacgg cggcgtggtg gaggtgcagc
     1681 aggactcctc cctgcaggac ggcaccttca tctacaaggt gaagttcaag ggcgtgaact
     1741 tccccgccga cggccccgta atgcagaaga agactgccgg ctgggagccc tccaccgaga
     1801 agctgtaccc ccaggacggc gtgctgaagg gcgagatctc ccacgccctg aagctgaagg
     1861 acggcggcca ctacacctgc gacttcaaga ccgtgtacaa ggccaagaag cccgtgcagc
     1921 tgcccggcaa ccactacgtg gactccaagc tggacatcac caaccacaac gaggactaca
     1981 ccgtggtgga gcagtacgag cacgccgagg cccgccactc cggctcccag ggactcagat
     2041 cttacaagga cgagctgtaa ctcgacctcg agacaaatgg cagtattcat ccacaatttt
     2101 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2161 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     2221 tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct
     2281 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     2341 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     2401 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     2461 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     2521 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     2581 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     2641 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     2701 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     2761 cctctctccc cagggggatc caccggagct taccatgacc gagtacaagc ccacggtgcg
     2821 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     2881 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     2941 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     3001 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     3061 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     3121 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     3181 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     3241 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     3301 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     3361 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     3421 gaccgaaagg agcgcacgac cccatgcatc ggtaccttta agaccaatga cttacaaggc
     3481 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     3541 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     3601 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     3661 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     3721 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     3781 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     3841 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3901 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     3961 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4021 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4081 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     4141 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     4201 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     4261 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     4321 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4381 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4441 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4501 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4561 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4621 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4681 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4741 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     4801 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4861 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4921 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4981 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5041 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5101 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5161 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5221 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5281 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5341 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5401 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5461 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5521 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5581 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5641 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5701 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5761 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5821 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5881 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5941 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6001 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6061 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6121 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6181 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6241 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6301 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6361 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6421 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6481 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6541 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6601 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6661 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6721 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     6781 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     6841 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     6901 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     6961 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     7021 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     7081 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     7141 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     7201 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     7261 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7321 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7381 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7441 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7501 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     7561 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     7621 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     7681 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     7741 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     7801 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     7861 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     7921 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     7981 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8041 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8101 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     8161 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     8221 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     8281 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     8341 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     8401 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     8461 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     8521 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     8581 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     8641 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     8701 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     8761 agatccattc gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgagcg
     8821 gccgccccct tcaccgaggg cctatttccc atgat
//