pGEX-2T-RIN

LOCUS       pGEX-2T-RIN                7020 bp DNA     circular SYN 09-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             918..938
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             942..3011
                     /codon_start=1
                     /label=RIN
                     /translation="MVMDATQSQQPSPQSVGREFVRQYYTLLNKAPNHLHRFYNHNSSY
                     IHGESKLVVGQREIHNRIQQLNFNDCHAKISQVDAQATLGNGVVVQVTGELSNDGQPMR
                     RFTQTFVLAAQSPKKYYVHNDIFRYQDLYIEDEQDGESRSENDEEHDVQVVGGTVDQAV
                     QVAGDVVAQQPQQQQAPPQPQAQVVVPQQVAPVAAGGAAPQQIFYPLPAGAAAGRPVAV
                     LPPAPQAAAVPLPAQFTVPPPQPIQNGVVSHEELQQQQVLVPPSVSPLVQQQQPTGTPV
                     LPIVPVPSAGFQQSPLVAPQLIQQQQQQQPAQLPPQQQQQQPIQQQQPLQQQQQPLAEP
                     EEVEVAPRQQKPPTPVEDFKTIHEQQQQEKYEAAKQQQNEPKTYANLFKSTSSSPSGFV
                     NAALQQQQQLQQQQQQQQSISSNTYNSSSATSGGGNGSQVSYSTTASSYNNSNGNSRLD
                     NGGPLPQRNNSIRNNKGDFEQRRSSNTQQFGDNQQLFLGNIPHHASEDDLREIFSRFGN
                     VLELRILSKAGNKVPPGMRSPLNYGFITYDDPEAVQKCLANCPLYFPENSPDGQKLNVE
                     EKKPRVRNDMVPRQQIGGNNLNNNMGRLGGNNGPQSRPMGNNNGSGGGMMRNNAVGNNL
                     RQGGGGGNGAPRLGGGGGFGQRQDNRTGGGNNQSNGPLRGAGNGQSGGGNYGRR"
     promoter        3323..3427
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3428..4288
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4459..5047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        5291..5368
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             5369..6451
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    6464..6485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6500..6530
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6538..6554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6562..6578
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6574..7020
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
     primer_bind     complement(6594..6610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatgaa aacctgtatt ttcagggatc catggtcatg gatgcgaccc
      961 aatcgcagca gccttcgccg caatctgtgg gacgcgagtt tgtccgccag tactacacgc
     1021 tgttgaacaa ggcgcccaac cacttgcatc gcttctataa ccacaactcg tcgtacatcc
     1081 atggagagtc caagctggtg gtcggtcagc gggagatcca caaccgaatc cagcagctga
     1141 acttcaacga ttgccacgcg aagatcagcc aggttgatgc ccaggccact ttgggcaacg
     1201 gtgtggtggt tcaggtcacc ggggagctat ccaatgatgg ccagccgatg cggcgtttta
     1261 cccagacgtt cgttctggcc gctcagtcgc cgaagaagta ctacgtgcac aacgacatct
     1321 tccgctatca ggatctctac atcgaggacg agcaggatgg cgagtcgcga tcggagaacg
     1381 atgaggagca cgatgtgcag gtcgtcggtg gcactgtgga tcaagctgtg caggtggccg
     1441 gcgatgtagt tgcccagcag ccgcagcaac agcaggctcc tccgcagcca caggctcagg
     1501 tcgtggtgcc ccagcaggta gctcctgtag cggccggtgg tgctgcccca cagcagatct
     1561 tctacccatt gccagcgggt gctgccgccg gacgtcctgt tgctgttttg ccaccagctc
     1621 cgcaagccgc tgctgttcca ctgcccgcgc agttcaccgt tccgccgcca cagcccatcc
     1681 agaacggtgt ggtgtcgcac gaggagctgc agcagcagca ggtgctggtc ccacccagcg
     1741 tgtctcccct ggtacagcag cagcagccga ccggcacgcc tgtgttgccc attgttccag
     1801 tgccaagcgc gggcttccaa cagtcgccac tggtggcacc acagctcatc cagcagcagc
     1861 agcagcagca accggcacag ctgcccccgc agcagcagca gcagcagcct atccagcagc
     1921 agcagccttt gcaacagcag caacaaccgc tggctgagcc cgaggaagtg gaggtggcgc
     1981 caaggcagca gaagcctccc acgcccgtcg aggatttcaa gaccattcac gagcagcagc
     2041 agcaggagaa gtacgaggcc gccaagcagc agcagaatga gcccaagacg tacgcgaatc
     2101 tcttcaagtc cacgtcctcc tcgcccagcg gctttgtgaa cgccgcactg cagcagcagc
     2161 aacagctcca gcagcaacag cagcagcagc agtccatcag cagcaacacc tataactctt
     2221 cctcggcaac ctcgggtggc ggtaatggca gtcaggttag ctactccacc accgcttcgt
     2281 cgtacaacaa cagcaacggc aactcgcgac tggacaacgg tggcccgctg ccgcagcgaa
     2341 acaactcgat ccgcaacaac aagggtgact ttgagcagcg tcgctcgagc aacacgcagc
     2401 agttcggtga taatcagcag ctgtttttgg gcaatatacc gcaccacgcc agcgaggatg
     2461 atctgcgcga gatcttctca cgcttcggaa atgtcctgga gctgcgcatt ttgtccaagg
     2521 ctggcaacaa ggtgccacca ggcatgcgca gtccgctgaa ctacggattc attacctacg
     2581 acgatcctga ggcggtgcaa aagtgcctgg cgaactgccc cctatacttc ccggagaact
     2641 cacccgatgg ccagaagttg aatgtggagg agaagaagcc gcgtgtccgc aacgacatgg
     2701 tgccgcgcca gcagatcggt ggcaacaacc tgaacaacaa catgggccgt ttgggcggca
     2761 acaatggtcc gcagtcgcgt ccgatgggca acaacaatgg cagcggcggt ggcatgatgc
     2821 gcaacaatgc cgttggcaac aatctgcgcc agggcggcgg cggaggaaac ggtgctcctc
     2881 gcctgggagg cggcggtgga ttcggacagc gccaggataa ccgcaccggt ggcggcaaca
     2941 accagtctaa cggaccgctg cgcggtgccg gaaatggaca gagtggtggt ggcaactacg
     3001 gacgtcgcta agaattcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga
     3061 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     3121 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     3181 agccatgacc cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct
     3241 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     3301 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3361 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3421 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     3481 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     3541 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     3601 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     3661 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     3721 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     3781 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3841 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3901 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     3961 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4021 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4081 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4141 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4201 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4261 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     4321 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4381 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4441 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4501 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4561 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     4621 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4681 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4741 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4801 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4861 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4921 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4981 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5041 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5101 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     5161 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     5221 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     5281 cataaattcc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc
     5341 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
     5401 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
     5461 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
     5521 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
     5581 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
     5641 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
     5701 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
     5761 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
     5821 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
     5881 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
     5941 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
     6001 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
     6061 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
     6121 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
     6181 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
     6241 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     6301 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     6361 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6421 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6481 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6541 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc
     6601 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6661 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6721 caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
     6781 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca
     6841 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg
     6901 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
     6961 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttggaatt
//