pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CTTNBP2-FLAG

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CTTNBP2-FLAG载体质粒基本信息

质粒名称: pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CTTNBP2-FLAG
出品公司: Wekwikgene
目录编号: 0001855
产品用途: 3' AAVLINK plasmid for CTTNBP2 expression
存储实验室: Zhonghua Lu Lab

载体骨架基本信息
载体名称: pAAV-MCS2
表达系统: 腺病毒
启动子: SCP1
拷贝数: 高拷贝
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: DH5alpha

插入基因信息
插入基因名称:
CTTNBP2
物种来源:
H.sapiens
密码子优化: 未优化

文献引用方法
参考文献部分:
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CTTNBP2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CTTNBP2-FLAG载体序列质粒序列多克隆位点信息

pAAVLINKv2-SCP1-Cre-UDeg3a-3-CTTNBP2-FLAG载体图谱

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CTTNBP2-FLAG质粒载体简介

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CTTNBP2-FLAG质粒序列载体序列

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CTTNBP2-FLAG               6554 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3267
                     /codon_start=1
                     /gene="CTTNBP2"
                     /gene_synonym="C7orf8; CORTBP2; Orf4"
                     /product="cortactin-binding protein 2 isoform 1"
                     /label=CTTNBP2
                     /note="isoform 1 is encoded by transcript variant 
                     1;CORTBP2, C7orf8"
                     /db_xref="GI:16975496"
                     /db_xref="CCDS:CCDS5774.1"
                     /db_xref="GeneID:83992"
                     /db_xref="HGNC:HGNC:15679"
                     /db_xref="MIM:609772"
                     /protein_id="NP_219499.1"
                     /translation="PQEGCLSSVTYASMIPLQMMQNYLRLVEQYHNVIFHGPEGSLQDY
                     IVHQLALCLKHRQMAAGFSCEIVRAEVDAGFSKEQLLDLFISSACLIPVKQSPSKKKII
                     IILENLEKSSLSELLRDFLAPLENRSTESPCTFQKGNGLSECYYFHENCFLMGTIAKAC
                     LQGSDLLVQQHFRWVQLRWDGEPMQGLLQRFLRRKVVNKFKGQAPSPCDPVCKIVDWAL
                     SVWRQLNSCLARLGTPEALLGPKYFLSCPVVPGHAQVTVKWMSKLWNGVIAPRVQEAIL
                     SRASVKRQPGFGQTTAKRHPSQGQQAVVKAALSILLNKAVLHGCPLPRAELDQHTADFK
                     GGSFPLSIVSSYNTCNKKKGESGAWRKVNTSPRRKSGRFSLPTWNKPDLSTEGMKNKTI
                     SQLNCNRNASLSKQKSLENDLSLTLNLDQRLSLGSDDEADLVKELQSMCSSKSESDISK
                     IADSRDDLRMFDSSGNNPVLSATINNLRMPVSQKEVSPLSSHQTTECSNSKSKTELGVS
                     RVKSFLPVPRSKVTQCSQNTKRSSSSSNTRQIEINNNSKEVNWNLHKNEHLEKPNK"
     misc_feature    2568..2573
                     /label=SDS
     misc_feature    3023..3028
                     /label=SDS
     CDS             3274..3339
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3343..3774
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3487..3492
                     /label=SDS
     repeat_region   3817..3957
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3817..3946
                     /label=AAV2 ITR
     repeat_region   3818..3958
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4032..4487
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4769..4873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4874..5734
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5905..6493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtcc tcaagaaggc
     1561 tgtctcagta gtgtgactta tgcctccatg atccctctcc agatgatgca gaactacctc
     1621 aggctggttg agcaatatca taatgtcatt ttccacggcc cagaaggaag cttgcaagac
     1681 tacatagtac atcagcttgc actctgcctg aagcacagac aaatggctgc aggattctcc
     1741 tgtgaaatag tgagagctga agtagatgct ggtttttcca aggaacagct actagacctg
     1801 ttcattagta gcgcttgtct gatcccagtg aaacaatctc ccagtaagaa gaaaatcatc
     1861 atcattttag aaaatttaga aaaatcttca ctgtcggagt tattgaggga ctttttggca
     1921 cctcttgaaa atcgcagcac tgaaagcccc tgcactttcc aaaaaggaaa tggactgtcc
     1981 gaatgttatt actttcatga gaactgcttt ctgatgggaa ccatcgccaa ggcctgtctc
     2041 cagggctccg acttgctggt gcagcagcat ttccgctggg tgcagctgcg gtgggatggc
     2101 gagcccatgc aaggactgct gcagaggttc ttacgaagga aagttgtgaa taagttcaaa
     2161 ggtcaggcgc cctccccctg cgatcctgtg tgcaagattg tcgactgggc tctgtccgtc
     2221 tggcgtcagc ttaactcctg cctggcccgc ttgggcacac ctgaagcact tcttggacca
     2281 aaatatttcc tgtcttgtcc tgtagttcct gggcatgccc aagtgacagt gaagtggatg
     2341 tctaagctgt ggaatggcgt catcgcaccc agagttcaag aagcaatatt gtcaagagcc
     2401 tctgtgaaaa gacaacctgg ctttgggcag acaactgcta aaagacaccc tagccaagga
     2461 cagcaggctg tggtcaaagc tgctctcagc atcttgctaa ataaagctgt actgcatggc
     2521 tgtcccctcc ccagagcaga gctagaccag catacagctg atttcaaagg aggaagtttc
     2581 cctttatcca tagtttccag ttataacact tgtaacaaga agaaaggaga gagtggtgcc
     2641 tggagaaagg tgaacaccag tcctcgcagg aagtctggcc gcttctcttt acccacctgg
     2701 aataagccag acctaagcac tgaaggtatg aaaaataaga ctatatcaca gctgaattgt
     2761 aacaggaatg cttctctgtc aaaacaaaag tctttagaga atgatctatc actgacgttg
     2821 aatttggatc agagactctc tctgggttca gatgacgaag cagatcttgt caaggaactt
     2881 cagagcatgt gctccagcaa gtctgagtct gatatcagca agattgctga ttccagggat
     2941 gatttaagga tgtttgatag ttctggaaac aaccctgtac tttcagcaac tattaataat
     3001 ctgagaatgc cagtgtcaca aaaggaggtc agtcctctca gcagccatca aactactgaa
     3061 tgcagcaaca gtaaatcaaa gactgagttg ggtgtttcaa gagttaaatc ttttcttcct
     3121 gttcctagaa gtaaagtcac ccagtgttcc cagaacacca aaagaagcag cagcagcagt
     3181 aatacaaggc aaatagaaat caacaacaac tcaaaagaag tgaattggaa cttacacaaa
     3241 aatgaacacc tagaaaaacc taacaaatgt acagactaca aagaccatga cggtgattat
     3301 aaagatcatg acatcgacta caaggatgac gatgacaagt aactcgagat aatcaacctc
     3361 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3421 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3481 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
     3541 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta
     3601 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt
     3661 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     3721 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct
     3781 ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     3841 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3901 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     3961 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     4021 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4081 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
     4141 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4201 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     4261 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4321 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
     4381 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
     4441 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     4501 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4561 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4621 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4681 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4741 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4801 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4861 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4921 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4981 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5041 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5101 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5161 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5221 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5281 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5341 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5401 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5461 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5521 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5581 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5641 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5701 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5761 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5821 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5881 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5941 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6001 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6061 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6121 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6181 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6241 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6301 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6361 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6421 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6481 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6541 ttttgctcac atgt
//

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