| 质粒名称: | pCDH_cgeMTS_GFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002296 |
| 产品用途: | Expression of MTS-GFP in CHO cells |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCDH |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
|
插入基因信息 |
|
| 插入基因名称: | cgeSOD2-GFP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Boosting energy metabolism and biosynthesis in diverse organisms by a common bacterial salvage lipoylation protein
Runqing Yang, Yingying Wang, Minghua Kong, Zhijuan Hu,Zhe Zhang, Kun Shen, Jiali Meng & An-Ping Zeng
Nature Communications,16,20250803
|
pCDH_cgeMTS_GFP
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCDH_cgeMTS_GFP载体质粒基本信息
pCDH_cgeMTS_GFP质粒图谱载体图谱和pCDH_cgeMTS_GFP载体序列质粒序列多克隆位点信息
pCDH_cgeMTS_GFP质粒载体简介
pCDH_cgeMTS_GFP质粒序列载体序列
LOCUS pCDH_cgeMTS_GFP 7248 bp DNA circular SYN 03-JUL-2026
DEFINITION contig1 circular.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7248)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7248
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 179..412
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 597..641
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 908..1024
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 1047..1350
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1351..1554
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 1605..1676
/label=cgeSOD2
CDS 1677..2390
/codon_start=1
/product="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
misc_feature 2430..3017
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2901..2912)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3090..3323
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 3395..3516
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 3556..3691
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(3724..3740)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3748..3764
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3772..3802)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3817..3838
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4126..4714)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4885..5745)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5746..5850)
/gene="bla"
/label=AmpR promoter
primer_bind 6324..6340
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 6355..6581
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 6582..6762
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 6809..6934
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
ORIGIN
1 aagtaagacc accgcacagc aagcggccac tgatcttcag acctggagga ggagatatga
61 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
121 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
181 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
241 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
301 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
361 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
421 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
481 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
541 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
601 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
661 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
721 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
781 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
841 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
901 ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
961 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa
1021 ttttatcgat actagttgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
1081 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
1141 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
1201 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
1261 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
1321 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
1381 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
1441 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
1501 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
1561 gtgaaccgtc agattctaga ccgctagcgc taccggtcgc caccatgcta tgtcgtgctg
1621 cctgctccgc tggccgccga ctcgctcccg ctgccactgc cgctggatcc cggcatgtga
1681 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
1741 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
1801 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
1861 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
1921 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
1981 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
2041 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
2101 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
2161 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
2221 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
2281 gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
2341 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
2401 gatctcgagc tcaagcttcg tagggatcca atcaacctct ggattacaaa atttgtgaaa
2461 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
2521 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
2581 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
2641 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
2701 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
2761 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
2821 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
2881 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
2941 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc
3001 tttggccgcc tccccgccgg tacctttaag accaatgact tacaaggcag ctgtagatct
3061 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata
3121 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
3181 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
3241 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
3301 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
3361 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
3421 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
3481 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
3541 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
3601 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
3661 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat
3721 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
3781 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
3841 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
3901 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
3961 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
4021 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
4081 ccagcaaaag gccaggaacc ttaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
4141 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
4201 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
4261 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
4321 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
4381 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
4441 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
4501 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
4561 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
4621 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
4681 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
4741 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
4801 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
4861 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
4921 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
4981 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
5041 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
5101 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
5161 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
5221 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
5281 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
5341 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
5401 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
5461 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
5521 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
5581 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
5641 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
5701 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
5761 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
5821 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
5881 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
5941 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
6001 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
6061 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
6121 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
6181 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
6241 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
6301 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctga cgcgtgtagt
6361 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
6421 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
6481 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
6541 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
6601 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
6661 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
6721 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
6781 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
6841 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
6901 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
6961 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
7021 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
7081 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
7141 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
7201 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaa
//
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