pQD699

LOCUS       pQD699                7572 bp DNA     circular SYN 16-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     212..232
                     /label=QL426-F
     primer_bind     216..236
                     /label=QL418-F
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=HIV-1 5 LTR
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1222..1227
                     /label=U6 terminator
     misc_feature    1251..1256
                     /label=U6 terminator
     misc_feature    1665..1670
                     /label=U6 terminator
     misc_feature    1684..1917
                     /label=RRE
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2620..2932
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2949..2965
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2994..3008)
                     /label=QL152-R
     protein_bind    3007..3040
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     3041..3088
                     /label=opqd1623
     primer_bind     3048..3068
                     /label=QL426-F
     primer_bind     3061..3089
                     /label=QL416-F
     primer_bind     3066..3089
                     /label=QL418-F
     misc_feature    3069..3349
                     /label=TERT
     primer_bind     3069..3088
                     /label=引物 3
     primer_bind     3069..3083
                     /label=引物 2
     primer_bind     complement(3311..3330)
                     /label=引物 1
     primer_bind     complement(3329..3366)
                     /label=QL419-R
     primer_bind     complement(3329..3350)
                     /label=QL417-R
     primer_bind     complement(3330..3349)
                     /label=引物 4
     primer_bind     complement(3347..3386)
                     /label=opqd1625
     primer_bind     3347..3386
                     /label=opqd1624
     CDS             join(3367..3919,4036..4352)
                     /codon_start=1
                     /product="E1A"
                     /label=E1A
                     /note="other spliced forms exist with larger introns"
                     /protein_id="DAA00639.1"
                     /translation="MRHIICHGGVITEEMAASLLDQLIEEVLADNLPPPSHFEPPTLHE
                     LYDLDVTAPEDPNEEAVSQIFPDSVMLAVQEGIDLLTFPPAPGSPEPPHLSRQPEQPEQ
                     RALGPVSMPNLVPEVIDLTCHEAGFPPSDDEDEEGEEFVLDYVEHPGHGCRSCHYHRRN
                     TGDPDIMCSLCYMRTCGMFVYSPVSEPEPEPEPEPEPARPTRRPKMAPAILRRPTSPVS
                     RECNSSTDSCDSGPSNTPPEIHPVVPLCPIKPVAVRVGGRRQAVECIEDLLNEPGQPLD
                     LSCKRPRP"
     primer_bind     complement(4332..4373)
                     /label=QL152-R
     primer_bind     complement(4332..4372)
                     /label=opqd1626
     protein_bind    4372..4405
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    4461..5049
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4469..4496)
                     /label=WPRE-R
     primer_bind     complement(5052..5068)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             5578..5758
                     /label=HIV-1 5 LTR
     rep_origin      complement(5802..6430)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6579..7439)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7440..7544)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag
     2641 gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc cctgccccgg
     2701 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
     2761 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataagag
     2821 atacaaatac taaattatta ttttaaaaaa cagcacaaaa ggaaactcac cctaactgta
     2881 aagtaattgt gtgttttgag actataaata tcccttggag aaaagccttg ttaacgcgcg
     2941 gtgaccctcg aggtcgacgg tatcgataag ctcgcttcac gagattccag caggtcgagg
     3001 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt ccaagcttaa
     3061 gcggccgcag tggattcgcg ggcacagacg cccaggaccg cgctccccac gtggcggagg
     3121 gactggggac ccgggcaccc gtcctgcccc ttcaccttcc agctccgcct cctccgcgcg
     3181 gaccccgccc cgtcccgacc cctcccgggt ccccggccca gccccctccg ggccctccca
     3241 gcccctcccc ttcctttccg cggccccgcc ctctcctcgc ggcgcgagtt tcaggcagcg
     3301 ctgcgtcctg ctgcgcacgt gggaagccct ggccccggcc acccccgcgg aattcgagct
     3361 cgcccgatga gacatattat ctgccacgga ggtgttatta ccgaagaaat ggccgccagt
     3421 cttttggacc agctgatcga agaggtactg gctgataatc ttccacctcc tagccatttt
     3481 gaaccaccta cccttcacga actgtatgat ttagacgtga cggcccccga agatcccaac
     3541 gaggaggcgg tttcgcagat ttttcccgac tctgtaatgt tggcggtgca ggaagggatt
     3601 gacttactca cttttccgcc ggcgcccggt tctccggagc cgcctcacct ttcccggcag
     3661 cccgagcagc cggagcagag agccttgggt ccggtttcta tgccaaacct tgtaccggag
     3721 gtgatcgatc ttacctgcca cgaggctggc tttccaccca gtgacgacga ggatgaagag
     3781 ggtgaggagt ttgtgttaga ttatgtggag caccccgggc acggttgcag gtcttgtcat
     3841 tatcaccgga ggaatacggg ggacccagat attatgtgtt cgctttgcta tatgaggacc
     3901 tgtggcatgt ttgtctacag taagtgaaaa ttatgggcag tgggtgatag agtggtgggt
     3961 ttggtgtggt aatttttttt ttaattttta cagttttgtg gtttaaagaa ttttgtattg
     4021 tgattttttt aaaaggtcct gtgtctgaac ctgagcctga gcccgagcca gaaccggagc
     4081 ctgcaagacc tacccgccgt cctaaaatgg cgcctgctat cctgagacgc ccgacatcac
     4141 ctgtgtctag agaatgcaat agtagtacgg atagctgtga ctccggtcct tctaacacac
     4201 ctcctgagat acacccggtg gtcccgctgt gccccattaa accagttgcc gtgagagttg
     4261 gtgggcgtcg ccaggctgtg gaatgtatcg aggacttgct taacgagcct gggcaacctt
     4321 tggacttgag ctgtaaacgc cccaggccat aagaattcgt cgagggacct aataacttcg
     4381 tatagcatac attatacgaa gttatacatg tttaagggtt ccggttccac taggtacaat
     4441 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4501 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4561 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4621 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4681 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4741 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4801 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     4861 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4921 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4981 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5041 cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca
     5101 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     5161 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     5221 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     5281 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     5341 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     5401 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     5461 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     5521 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     5581 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5641 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5701 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     5761 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5821 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5881 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5941 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6001 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6061 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6121 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6181 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6241 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6301 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6361 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6421 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6481 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6661 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6721 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6781 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6841 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6901 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6961 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7021 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7081 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7141 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7201 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7261 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7321 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7381 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7441 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7501 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7561 gtgccacctg ac
//