pYK65

LOCUS       pYK65               7415 bp DNA     circular SYN 13-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    70..147
                     /label=SV40 ORI
     CDS             289..1080
                     /label=Kan/neoR
     misc_feature    1254..1384
                     /label=SV40 early polyadenylation signal
     primer_bind     1358..1377
                     /label=olyl382
     primer_bind     complement(1420..1440)
                     /label=M13-rev
     misc_binding    complement(1446..1468)
                     /label=LacO
     promoter        complement(1473..1502)
                     /label=lac
     misc_feature    complement(1767..2437)
                     /label=pUC origin
     CDS             complement(2585..3244)
                     /label=AmpR
     primer_bind     2901..2912
                     /label=oyk03-03-F
     primer_bind     2901..2912
                     /label=oYK04-ns3a-F
     primer_bind     2901..2912
                     /label=oYK153_NS3a_F
     promoter        complement(3484..3512)
                     /label=Amp prom
     misc_feature    3810..4397
                     /label=CMV
     primer_bind     4441..4460
                     /label=T7
     primer_bind     4459..4505
                     /label=oYK319_NLSPB1_F
     primer_bind     4459..4500
                     /label=oYK52-MTS-PB1-AG-F
     primer_bind     4459..4494
                     /label=oYK33-35 PB1-AG-F
     primer_bind     4459..4487
                     /label=oYK30-NS3a_Head-F
     primer_bind     4459..4478
                     /label=oYK06-ANR-mEGFP-F
     regulatory      4479..4488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4485..4790
                     /label=PB1
     primer_bind     4488..4505
                     /label=oYK164_PB_F
     misc_feature    4791..5171
                     /label=Long_Linker
     primer_bind     complement(4888..4928)
                     /label=EGFP-TM-2-reverse
     primer_bind     complement(5038..5078)
                     /label=EGFP-TM-2-reverse
     misc_feature    5172..5846
                     /label=AG
     primer_bind     5620..5630
                     /label=oSCL1104
     primer_bind     complement(5834..5869)
                     /label=oYK65-AG-R
     misc_feature    5847..5854
                     /label=Short_Linker
     primer_bind     5850..5864
                     /label=oYK65-NS3a-F
     primer_bind     5850..5861
                     /label=oYK29-NS3a-F
     primer_bind     complement(5861..5886)
                     /label=oYK03-02-R
     primer_bind     complement(5861..5878)
                     /label=oYK283_PB-R
                     /note="TTCAAGCTTTGTTCTCGAGTTTCCC"
     primer_bind     complement(5863..5877)
                     /label=EGFP-TM-2-reverse
     primer_bind     5867..5897
                     /label=oyk03-03-F
     misc_feature    5871..5876
                     /label=linker
     primer_bind     5874..5912
                     /label=oSCL1082
     misc_feature    5875
                     /label=kozak
     primer_bind     5876..5897
                     /label=oYK04-ns3a-F
     misc_feature    5876
                     /label=linker
     CDS             5877..6464
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     5877..5897
                     /label=oYK153_NS3a_F
     primer_bind     5877..5895
                     /label=oYK25-NS3a-F
     primer_bind     5877..5894
                     /label=oYK30-NS3a_Head-F
     primer_bind     5894..5952
                     /label=oSCL1083
     primer_bind     5933..5991
                     /label=oSCL1084
     primer_bind     5970..6028
                     /label=oSCL1085
     primer_bind     6007..6065
                     /label=oSCL1086
     primer_bind     complement(6035..6054)
                     /label=oSCL1106
     primer_bind     6036..6074
                     /label=oSCL1104
     primer_bind     complement(6271..6329)
                     /label=oSCL1105
     primer_bind     complement(6311..6382)
                     /label=oSCL1087
     primer_bind     6313..6334
                     /label=oSCL1107
     primer_bind     6364..6382
                     /label=oSCL1088
     primer_bind     complement(6446..6493)
                     /label=oSCL1089
     primer_bind     complement(6449..6496)
                     /label=oYK30-NS3a_head-R
     primer_bind     complement(6452..6470)
                     /label=oYK342_NS3a_R
     primer_bind     complement(6465..6513)
                     /label=oYK03-03-R
     primer_bind     complement(6465..6484)
                     /label=oSCL1130
     primer_bind     complement(6473..6493)
                     /label=olyl382
     primer_bind     complement(6494..6516)
                     /label=oYK57-MCP-mCherry-R
     primer_bind     complement(6494..6513)
                     /label=oYK25-NS3a-R
     primer_bind     complement(6518..6537)
                     /label=oSCL1089
     terminator      6590..6817
                     /label=bGH PA
     misc_feature    6863..7291
                     /label=f1 origin
     misc_feature    join(7296..7415,1..224)
                     /label=SV40 early promoter and origin
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
      241 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
      301 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
      361 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
      421 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
      481 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
      541 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
      601 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
      661 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
      721 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
      781 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
      841 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
      901 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
      961 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     1021 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     1081 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     1141 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     1201 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     1261 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1321 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1381 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     1441 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1501 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1561 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1621 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1681 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1741 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1801 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1861 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1921 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1981 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2041 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2101 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2161 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2221 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2281 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2341 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2401 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2461 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2521 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2581 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2641 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2701 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2761 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2821 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2881 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2941 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3001 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3061 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3121 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3181 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3241 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3301 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3361 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3421 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3481 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3541 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     3601 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     3661 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     3721 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     3781 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     3841 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     3901 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     3961 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     4021 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     4081 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     4141 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     4201 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     4261 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     4321 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     4381 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     4441 taatacgact cactataggg agacccaagc tggctagcgc caccatggcg tcgctcaccg
     4501 tgaaggccta ccttctgggc aaggaggacg cggcgcgcga gattcgccgc ttcagcttct
     4561 gttgcagccc cgagcctgag gcggaagccg aggctgcggc gggtccggga ccctgcgagc
     4621 ggctgctgag ccgggtggcc gccctgttcc ccgcgctgcg gcctggcggc ttccaggcgc
     4681 actaccgcga tgaggacggg gacttggttg ccttttccag tgacgaggaa ttgacaatgg
     4741 ccatgtccta cgtgaaggat gacatcttcc gaatctacat taaagagaaa accggttctg
     4801 ggagtggcgg tagtggtgct ggaggcagcg caggctctgg agcaggcggc agtgccggaa
     4861 gtggcgctgg agggtctgcc ggatctggag ccggtggttc tgccggctcc ggcgctggtg
     4921 ggagcgcttc cggaagtagt cccggttctg ggagtggcgg tagtggtgct ggaggcagcg
     4981 caggctctgg agcaggcggc agtgccggaa gtggcgctgg agggtctgcc ggatctggag
     5041 ccggtggttc tgccggctcc ggcgctggtg ggagcgcttc cggaagtagt cccggtaatt
     5101 ccgctgacgg cggcggagga tcgggtggta gtggtggttc aggaggagga tcgacccaag
     5161 gaggatccat ggtgagcgtg atcaagcccg agatgaagat caagctgtgc atgaggggca
     5221 ccgtgaacgg ccacaacttc gtgatcgagg gcgagggcaa gggcaacccc tacgagggca
     5281 cccagatcct ggacctgaac gtgaccgagg gcgcccccct gcccttcgcc tacgacatcc
     5341 tgaccaccgt gttccagtac ggcaacaggg ccttcaccaa gtaccccgcc gacatccagg
     5401 actacttcaa gcagaccttc cccgagggct accactggga gaggagcatg acctacgagg
     5461 accagggcat ctgcaccgcc accagcaaca tcagcatgag gggcgactgc ttcttctacg
     5521 acatcaggtt cgacggcgtg aacttccccc ccaacggccc cgtgatgcag aagaagaccc
     5581 tgaagtggga gcccagcacc gagaagatgt acgtgaggga cggcgtgctg aagggcgacg
     5641 tgaacatggc cctgctgctg gagggcggcg gccactacag gtgcgacttc aagaccacct
     5701 acaaggccaa gaaggacgtg aggctgcccg actaccactt cgtggaccac aggatcgaga
     5761 tcctgaagca cgacaaggac tacaacaagg tgaagctgta cgagaacgcc gtggccaggt
     5821 acagcatgct gcccagccag gccaagggtg gcggtgggag cggaggcggt gggtccaaga
     5881 agaagggcag cgtggtgatc gtgggcagaa tcaacctgag cggcgacacc gcctacagcc
     5941 agcagaccag aggcctggag ggctgccagg agaccagcca gaccggcaga gacaagaacc
     6001 aggtggaggg cgaggtgcag gtggtgagca ccgccaccca gagcttcctg gccaccagca
     6061 tcaacggcgt gctgtggacc gtgtaccacg gcgccggcac cagaaccatc gccagcccca
     6121 agggccccgt gacccagatg tacacgaacg tggacaagga cttagtgggc tggcaggccc
     6181 cccaaggcag cagaagcctg accccctgca cctgcggcag cagcgacctg tacctggtga
     6241 ccagacacgc cgacgtgatc cccgtgagag gaagaggcga cagcagaggc agcctgctga
     6301 gccccagacc catcagctat ctgaaaggct caagcggcgg ccccctgttg tgtcccgccg
     6361 gccatgccgt gggcatcttc agagccgccg tgagcaccag aggcgtggcc aaggccgtgg
     6421 acttcatccc cgtggagagc ctggagacca ccatgagaag ccccgctagc tagaaattct
     6481 gcagatatcc agcggatcca ctagtccagt gtggtggaat tctgcagata tccagcacag
     6541 tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     6601 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6661 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6721 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6781 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6841 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6901 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6961 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7021 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7081 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7141 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7201 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7261 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     7321 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7381 cagcaaccag gtgtggaaag tccccaggct cccca
//