pHGZ-HKZ

LOCUS       pHGZ-HKZ             7383 bp DNA     circular SYN 22-JUL-2026
DEFINITION  2761_TSM20260706-0571-01212_20260707-0571-BAN14-4_H06.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        21..96
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      100..119
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     CDS             505..1365
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1536..2124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2462..2481
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     misc_RNA        complement(2485..2560)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2906..3211
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPG*RMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(3569..3837)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     gene            3844..5419
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        3844..4187
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4188..5216
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      5222..5419
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      5922..6799
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        7115..7383
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
ORIGIN
        1 caagttgacc agtgccgttc gttttagagc tagaaatagc aagttaaaat aaggctagtc
       61 cgttatcaac ttgaaaaagt ggcaccgagt cggtggtgct ttttttgttt tttatgtctc
      121 gtcacatcgg ataataatga tggcagccat tgtagaagtg ccttttgcat ttctagtctc
      181 tttctcggtc tagctagttt tactacatcg cgaagataga atcttagatc acactgcctt
      241 tgctgagctg gatcaataga gtaacaaaag agtggtaagg cctcgttaaa ggacaaggac
      301 ctgagcggaa gtgtatcgta cagtagacgg agtatactag gtatagtcta tagtccgtgg
      361 aattaattct catgtttgac agcttatcat cgataagcta gcttttcaaa aaactgtatt
      421 ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt
      481 attacccatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      721 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      781 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      841 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      901 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      961 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1021 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1081 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1201 tctggagccg gtgagcgtgg gtcacgcggt atcattgcag cactggggcc agatggtaag
     1261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1681 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2221 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2281 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2341 tggccgattc attaatgcag ggcctgcggc cctctagatg catgctcgag cggccgccag
     2401 tgtgatggat atctgcagaa ttcgtcgacg agctcggtac caagcttgga tcctaggatc
     2461 tagacataaa aaacaaaaaa agcaccaccg actcggtgcc actttttcaa gttgataacg
     2521 gactagcctt attttaactt gctatttcta gctctaaaac cgagacccag ggatccggct
     2581 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     2641 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac
     2701 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     2761 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     2821 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     2881 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     2941 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggtgacggat
     3001 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     3061 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     3121 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     3181 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     3241 tgcaggtcga tacagtagaa attacagaaa ctttatcacg tttagtaagt atagaggctg
     3301 aaaatccaga tgaagccgaa cgacttgtaa gagaaaagta taagagttgt gaaattgttc
     3361 ttgatgcaga tgattttcag gactatgaca ctagcgtata tgaataggta gatgttttta
     3421 ttttgtcaca caaaaaagag gctcgcacct ctttttctta tttcttttta tgatttaata
     3481 cggcattgag gacaatagcg agtaggctgg atacgacgat tccgtttgag aagaacattt
     3541 ggaaggctgt cggtcgacct gggtctcaga tcatttatct ttcactgcgg agaagtttcg
     3601 aacgccgaaa catgcgcacc aactttcact tctacagcgt ttgaccaaaa tcttttgaac
     3661 agaacattgt agggtgtgaa aaaatgcgca cctttaccgc tagcccaaga gggcactaca
     3721 aaatctagag ttgtacttca aacgtacatg taatcacctt gtatatactc gaaagaaaac
     3781 atcaagtttc tgtataaata tgagtgaaag cataatcata cattatcttt tcaaagaact
     3841 agtgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
     3901 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
     3961 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcggacctgc
     4021 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
     4081 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
     4141 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg ggtaaaaagc
     4201 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     4261 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     4321 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     4381 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     4441 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     4501 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     4561 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     4621 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     4681 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     4741 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     4801 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     4861 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     4921 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     4981 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     5041 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     5101 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     5161 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataatcag
     5221 tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat
     5281 tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat
     5341 catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg
     5401 aatgctggtc gctatactgg gatctctcac ttaatcttct gtactctgaa gaggagtggg
     5461 aaataccaag aaaaacatca aactcgaatg attttcccaa acccctacca caagatattc
     5521 atcagggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5581 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5641 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5701 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5761 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5821 cgaattttaa caaaatatta acgcttacaa tttaaatatt tgcttataca atcttcctgt
     5881 ttttggggct tttctgatta tcaaccgggg tggagcttcc cattgcgaat accgcttcca
     5941 caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc
     6001 tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg
     6061 tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga
     6121 atcgaaaaca atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga
     6181 agaaaccgtt cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca
     6241 caaagtatgc gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa
     6301 aatgcacccg cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt
     6361 atggaagaga aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca
     6421 aaaagttatc aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat
     6481 gctttgttag aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag
     6541 attctttgtt ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc
     6601 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag
     6661 cacagattct tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc
     6721 agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat
     6781 gaagcacaga tgcttcgtta acaaagatat gctattgaag tgcaagatgg aaacgcagaa
     6841 aatgaaccgg ggatgcgacg tgcaagatta cctatgcaat agatgcaata gtttctccag
     6901 gaaccgaaat acatacattg tcttccgtaa agcgctagac tatatattat tatacaggtt
     6961 caaatatact atctgtttca gggaaaactc ccaggttcgg atgttcaaaa ttcaatgatg
     7021 ggtaacaagt acgatcgtaa atctgtaaaa cagtttgtcg gatattaggc tgtatctcct
     7081 caaagcgtat tcgaatatca ttgagaagct gcagtctttg aaaagataat gtatgattat
     7141 gctttcactc atatttatac agaaacttga tgttttcttt cgagtatata caaggtgatt
     7201 acatgtacgt ttgaagtaca actctagatt ttgtagtgcc ctcttgggct agcggtaaag
     7261 gtgcgcattt tttcacaccc tacaatgttc tgttcaaaag attttggtca aacgctgtag
     7321 aagtgaaagt tggtgcgcat gtttcggcgt tcgaaacttc tccgcagtga aagataaatg
     7381 atc
//