| 质粒名称: | px330-U6-Nme2-C.NR gRNA(Bsa1)-CAG-Nme2-P2A-mCherry |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002334 |
| 产品用途: | A human codon-optimized Nme2-C.NR and chimeric guide RNA expression plasmid. |
| 存储实验室: | Guanghai Xiang lab |
|
载体骨架基本信息 |
|
| 载体名称: | px330 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
|
插入基因信息 |
|
| 插入基因名称: | Nuclease, mCherry |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing.
Xin C., Xiang G., Cao S., Wang Y., Yuan S., Liu X., Huo Y., Sun J., Wan X., Liu D., Hong J., Hu J., Wang H.
Genome Biol. 2026 (2026)
|
px330-U6-Nme2-C.NR gRNA(Bsa1)-CAG-Nme2-P2A-mCherry
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
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px330-U6-Nme2-C.NR gRNA(Bsa1)-CAG-Nme2-P2A-mCherry载体质粒基本信息
px330-U6-Nme2-C.NR gRNA(Bsa1)-CAG-Nme2-P2A-mCherry质粒图谱载体图谱和px330-U6-Nme2-C.NR gRNA(Bsa1)-CAG-Nme2-P2A-mCherry载体序列质粒序列多克隆位点信息
px330-U6-Nme2-C.NR gRNA(Bsa1)-CAG-Nme2-P2A-mCherry质粒载体简介
px330-U6-Nme2-C.NR gRNA(Bsa1)-CAG-Nme2-P2A-mCherry质粒序列载体序列
LOCUS px330-U6-Nme2-C.NR gRNA(Bsa1)-CAG-Nme2-P2A-mCherry 8326 bp DNA circular SYN 20-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8326)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Aug 26, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8326
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 1..589
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 651..899
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
misc_RNA 948..1040
/label=Nm tracrRNA
/note="trans-activating CRISPR RNA for the Neisseria
meningitidis CRISPR/Cas9 system (Hou et al., 2013)"
enhancer 1058..1343
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 1345..1622
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
promoter 1469..1489
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
intron 1623..1850
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1862..1871
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1868..5167
/codon_start=1
/label=Nme2-C.NR
/translation="MAPKKKRKVGIHGVPAATSAAFKPNPINYILGLDIGIASVGWAMV
EIDEEENPIRLIDLGVRVFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLL
KREGVLQAADFDENGLITSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRK
NEGETAAKELGALLKGVANNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFS
RKDLQAELILLFEKQKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTLEPT
EPKAAKNTYTAERFIWLTKLNNLRILEQGSERPLTDTERSTLMDEPYRKSKLTYAQARK
LLGLEDTAFFKGLRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSSELQDE
IGTAFSLFKTDEDITGRLKDRVQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKR
YDEACAEIYGVHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPA
RIHIETAREVGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRL
YEQQHGKCLYSGKEINLVRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQ
TPYEYFNGKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKECNLNDTRYVNRF
LCQFVADHILLTGKGKRRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTV
AMQQKITRFVRYKEMNAFDGKTIDKETGKVLHQKTHFPQPWEFFAQEVMIRVFGKPDGK
PEFEEADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGAHKDTLRSAKRFV
KHNEKISVKRVWLTEIKLADLENMVNYKNGREIELYEALKARLEAYGGNAKQAFDPKDN
PFYKKGGQLVKAVRVEKTQKSGVLLNKKNAYTIADNGDMVRVDVFCKVDKKGKNQYFIV
PIYAWQVAENILPDIDCKGYRIDDSYTFCFSLHKYDLIAFQKDEKSKVEFAYYINCDSS
SGGFYLAWHDKGSREQRFRISTQNLALIQKYQVNELGKEIRPCRLKKRPPVR"
CDS 1874..1894
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5168..5215
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 5225..5281
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 5282..5992
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
misc_feature 5298..5303
/label=SDS
polyA_signal 6023..6230
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 6239..6379
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 6239..6368
/label=AAV2 ITR
rep_origin 6454..6909
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 7191..7295
/gene="bla"
/label=AmpR promoter
CDS 7296..8156
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat
661 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
721 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
781 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
841 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
901 gagaccacgg caggtctcag ttgtagctcc ctttctcatt tcggaaacga aatgagaacc
961 gttgctacaa taaggccgtc tgaaaagatg tgccgcaacg ctctgcccct taaagcttct
1021 gctttaaggg gcatcgttta ttttttctag aggtacccgt tacataactt acggtaaatg
1081 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag
1141 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
1201 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
1261 cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
1321 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
1381 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
1441 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
1501 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
1561 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
1621 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
1681 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
1741 ctccgggctg taattagctg agcaagaggt aagggtttaa gggatggttg gttggtgggg
1801 tattaatgtt taattacctg gagcacctgc ctgaaatcac tttttttcag gttggaccgg
1861 tgccaccatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccac
1921 tagtgccgcc ttcaagccca accccatcaa ctacatcctg ggcctggaca tcggcatcgc
1981 cagcgtggga tgggctatgg tggaaatcga cgaggaggag aaccccatca gactgatcga
2041 cctgggcgtg agagtgttcg agagagccga ggtgcctaag accggagaca gcctggctat
2101 ggccagaaga ctggccagaa gcgtgagaag actgaccaga agaagagccc acagactgct
2161 gagagccaga agactgctga agagagaggg cgtgctgcag gccgccgatt ttgatgaaaa
2221 tggcctgatc acaagcctgc ccaacacccc ctggcaactg agagccgctg ctcttgatag
2281 aaaactgacc cctctggagt ggagcgccgt gctgctgcat ctgattaaac acagaggcta
2341 cctgagccag agaaagaacg agggcgaaac cgccgctaaa gagctgggag ctctgcttaa
2401 aggcgtggcc aataacgccc acgctctgca aacaggcgac tttagaaccc ctgctgagct
2461 ggccttaaac aagttcgaga aggagagcgg ccacatcaga aaccagagag gcgactacag
2521 ccacaccttc agcagaaagg acctgcaggc cgagctgatc ctgctgtttg agaagcagaa
2581 ggagttcggc aacccccacg tgagcggagg actgaaagaa ggaattgaaa ccctgctgat
2641 gacccagagg cccgccctga gcggagatgc tgttcaaaag atgctgggcc attgcaccct
2701 ggaacccaca gagcctaaag ccgctaaaaa cacctatacc gccgaaagat tcatctggct
2761 gaccaagctg aacaacctga gaatcctgga gcagggcagc gaaagacccc tgacagatac
2821 agaaagaagc accctgatgg acgagcccta cagaaagagc aagctgacct acgcccaggc
2881 cagaaagctg ctgggactgg aagataccgc cttcttcaag ggcctgagat acggcaagga
2941 caacgccgag gctagcaccc tgatggagat gaaggcctac cacgccatca gcagagccct
3001 ggaaaaagag ggcctgaagg acaagaaaag ccccctgaac ctgagcagcg agctgcagga
3061 tgaaattggc acagctttta gcctgttcaa gaccgacgag gacatcaccg gcagactgaa
3121 ggacagagtg cagcccgaaa tcctggaggc cctgttaaag catattagct tcgacaagtt
3181 cgtgcagatc agcctgaagg ccctgagaag aatcgtgccc ctgatggagc agggcaagag
3241 atacgacgag gcctgcgctg aaatttacgg cgtgcattat ggcaaaaaga acaccgagga
3301 gaagatctac ctgcccccca tccccgctga tgaaattaga aaccctgtgg tgctgagagc
3361 cctgagccag gctagaaaag tgatcaacgg cgtggtgaga agatacggca gccccgctag
3421 aatccacatc gaaaccgcta gagaggtggg caagagcttc aaggacagaa aggagatcga
3481 gaagagacag gaggagaaca gaaaggacag agagaaggcc gccgccaagt tcagagagta
3541 cttccccaat ttcgtgggcg agcccaaaag caaggacatc ctgaagctga gactgtacga
3601 gcagcagcac ggcaagtgcc tgtatagcgg aaaagagatc aacctggtga gactgaacga
3661 gaagggctac gtggagatcg accacgccct gccttttagc agaacatggg atgatagctt
3721 caacaacaag gtgctggtgc tgggcagcga gaaccagaat aagggcaacc agacccccta
3781 cgagtacttc aacggcaagg acaacagcag agagtggcag gagttcaagg ccagagtgga
3841 gacaagcaga ttccccagaa gcaagaagca gagaatcctg ctgcagaagt tcgacgagga
3901 cggcttcaag gagtgcaacc tgaacgacac cagatacgtg aacagattcc tgtgccagtt
3961 cgtggccgac cacatcctgt taacaggcaa gggaaaaaga agagtgttcg ccagcaacgg
4021 ccagatcacc aacctgctga gaggcttctg gggcctgaga aaggtgagag ccgaaaacga
4081 cagacaccac gccctggatg ccgtggtggt ggcttgttct acagtggcca tgcagcagaa
4141 gattaccaga ttcgtgagat acaaggagat gaacgccttc gacggcaaga ccatcgacaa
4201 ggagacaggc aaagtgctgc accagaaaac ccacttcccc cagccctggg aattcttcgc
4261 ccaagaggtg atgatcagag tgttcggcaa gcccgacggc aagcccgaat ttgaagaagc
4321 tgatacacct gagaaactga gaaccctgct ggccgaaaaa ctgagcagca gacctgaggc
4381 cgtgcatgag tatgtgaccc ccctttttgt gagcagagcc cccaatagaa agatgagcgg
4441 cgcccacaaa gacaccctga gaagcgctaa aagattcgtg aagcacaacg agaagatcag
4501 cgtgaagaga gtgtggctga ccgagatcaa gctggccgac ctggaaaata tggtgaacta
4561 caagaacggc agagagatcg agctgtacga ggccctgaaa gccagactgg aagcctatgg
4621 aggaaacgcc aagcaggcct tcgaccccaa agataatcct ttctacaaga agggcggcca
4681 gctggtgaag gccgtgagag ttgagaaaac ccagaagagc ggcgtgctgc tgaataagaa
4741 gaacgcctac accatcgccg acaacggcga catggtgaga gtggacgtgt tctgcaaggt
4801 ggacaagaag ggcaagaacc agtacttcat cgtgcccatc tacgcctggc aggtggccga
4861 aaatattctg cccgatatcg actgcaaggg ctacagaatc gacgacagct acaccttctg
4921 cttcagcctg cacaagtacg acctgatcgc cttccagaag gacgagaaga gcaaggtgga
4981 gttcgcctac tacatcaact gcgacagcag cagcggcggc ttttacctgg cttggcatga
5041 caagggaagc agagagcaga gattcagaat cagcacccag aacctggccc tgatccagaa
5101 gtaccaggtg aacgagctgg gcaaggagat cagaccctgc agactgaaga aaagaccccc
5161 cgtgagaaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagggaag
5221 cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc
5281 tatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
5341 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
5401 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
5461 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
5521 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
5581 cgtgatgaac ttcgatgacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
5641 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
5701 aatgcagaag aaaaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
5761 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
5821 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
5881 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
5941 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aagaattcct
6001 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
6061 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
6121 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
6181 caggacagca agggggagga ttgggaagag aatagcaggc atgctgggga gcggccgcag
6241 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
6301 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
6361 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
6421 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
6481 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
6541 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
6601 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
6661 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
6721 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
6781 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
6841 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
6901 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
6961 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
7021 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
7081 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
7141 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
7201 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
7261 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
7321 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
7381 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
7441 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
7501 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
7561 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
7621 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
7681 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
7741 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
7801 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
7861 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
7921 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
7981 ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg tatcattgca gcactggggc
8041 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
8101 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
8161 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
8221 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
8281 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
