pBT3S-bk1HP

LOCUS      pBT3S-bk1HP              7621 bp DNA     circular SYN 18-MAR-2026
DEFINITION  pBT3S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1435..1453
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1474..1764
                     /label=CYC1 promoter
     CDS             1770..1829
                     /codon_start=1
                     /label=SUC2
                     /translation="MMLLQAFLFLLAGFAAKISA"
     primer_bind     complement(1888..1904)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1917..2051
                     /codon_start=1
                     /label=Cub
                     /translation="MSGGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
                     "
     CDS             2076..2681
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             2700..2933
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     terminator      2938..3185
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(3204..3222)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3232..3248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3389..3844
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4145..4552
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4553..5647
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(6366..7181)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagctcgc ttgcctgtaa
      781 cttacacgcg cctcgtatct tttaatgatg gaataatttg ggaatttact ctgtgtttat
      841 ttatttttat gttttgtatt tggattttag aaagtaaata aagaaggtag aagagttacg
      901 gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc gtactttaca
      961 tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt aacgataagt
     1021 aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt
     1081 cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt tggcgatccc
     1141 cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt tcttttttta
     1201 ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt atttttatag
     1261 cacgtgatga aaaggaccca ggtggcactt ttcggggaaa tgtgcgcgga acccctaagc
     1321 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1381 ctggcacgac aggtttcccg actggaaagc cgcggatgat tacgccaagc gcgcaattaa
     1441 ccctcactaa agggaacaaa agctggagct catttggcga gcgttggttg gtggatcaag
     1501 cccacgcgta ggcaatcctc gagcagatcc gccaggcgtg tatatatagc gtggatggcc
     1561 aggcaacttt agtgctgaca catacaggca tatatatatg tgtgcgacga cacatgatca
     1621 tatggcatgc atgtgctctg tatgtatata aaactcttgt tttcttcttt tctctaaata
     1681 ttctttcctt atacattagg acctttgcag cataaattac tatacttcta tagacacgca
     1741 aacacaaata cacacactaa tcaagctata tgatgctttt gcaggcattc cttttccttt
     1801 tggctggttt tgcagccaaa atatctgcaa tgcaggtcga ctctcgagga tccaagcttc
     1861 tgcagggtac ccggtatcga tttaaatcga taccgtcgac ctcgataccg tcgaccatgt
     1921 cgggggggat ccctccagat caacaaagat tgatctttgc cggtaagcag ctagaagacg
     1981 gtagaacgct gtctgattac aacattcaga aggagtccac cttacatctt gtgctaaggc
     2041 taagaggtgg tatgcacaga tcagcttgcg gccgcatgaa agcgttaacg gccaggcaac
     2101 aagaggtgtt tgatctcatc cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg
     2161 cggaaatcgc gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa catctgaagg
     2221 cgctggcacg caaaggcgtt attgaaattg tttccggcgc atcacgcggg atccgtctgt
     2281 tgcaggaaga ggaagaaggg ttgccgctgg taggtcgtgt ggctgccggt gaaccacttc
     2341 tggcgcaaca gcatattgaa ggtcattatc aggtcgaccc ttccttattc aagccgaatg
     2401 ctgatttcct gctgcgcgtc agcgggatgt cgatgaaaga tatcggcatt atggatggtg
     2461 acttgctggc agtgcataaa actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta
     2521 ttgatgacga ggttaccgtt aagcgcctga aaaaacaggg caataaagtc gaactgttgc
     2581 cagaaaatag cgagtttaaa ccaattgtcg tagatcttcg tcagcagagc ttcaccattg
     2641 aagggctggc ggttggggtt attcgcaacg gcgactggct ggaattcccg gggatctggg
     2701 cccccccgac cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtggcga
     2761 tggcgcatgc cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc
     2821 cgggtccggg atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact
     2881 tcgagtttga gcagatgttt accgatgccc ttggaattga cgagtacggt gggtaggtca
     2941 tgtaattagt tatgtcacgc ttacatacac gccctccccc cacatccgct ctaaccgaaa
     3001 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag
     3061 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg
     3121 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa
     3181 tttgcggccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     3241 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3301 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     3361 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     3421 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3481 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3541 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3601 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3661 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3721 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3781 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     3841 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatcga
     3901 ccggtcgagg agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg
     3961 gaatcccaac aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc
     4021 ttgttcatgt gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca
     4081 agtaattggt tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc
     4141 agttaactgt gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc
     4201 attatttttt tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt
     4261 cgatgactgg aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac
     4321 tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata
     4381 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
     4441 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
     4501 cttttacatt tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc
     4561 ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc
     4621 cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa
     4681 tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct
     4741 ggaagcctcc aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg
     4801 taccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta
     4861 cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa
     4921 gccacaattt gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta
     4981 ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac
     5041 cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc
     5101 accattgcct atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag
     5161 aaaaactgtg gaggaaacca tcaagaacga attccctaca ttgaaggttc aacatcaatt
     5221 gattgattct gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat
     5281 caccagcaac atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt
     5341 gggtttgttg ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt
     5401 gtacgaacca tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc
     5461 cactatcttg tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa
     5521 ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg
     5581 tggttccaac agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct
     5641 tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa atgaaattca
     5701 taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat
     5761 acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta
     5821 agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat
     5881 attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat
     5941 gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa
     6001 aacactcaat gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc
     6061 cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag
     6121 tcgatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     6181 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     6241 agacaagctg tgaccgtctc cgggaccaca tgtggatgat ccagttcgat ttattcaaca
     6301 aagccgccgt cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat
     6361 tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     6421 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     6481 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     6541 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     6601 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
     6661 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     6721 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     6781 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     6841 ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat
     6901 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     6961 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     7021 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     7081 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     7141 cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
     7201 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
     7261 catcagagat tttgagacac aacgtggctt actaggatcg atccgacatg tggcggtatc
     7321 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7381 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7441 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7501 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7561 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7621 t
//