pB35C-epegRNA-ePPEplus

LOCUS       pB35C-epegRNA-ePPEplus            18379 bp DNA     circular SYN 17-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18379)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..18379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..26
                     /label=RB
                     /note="T-DNA repeat"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     106..125
                     /label=LHC-265-3F
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    274..1760
                     /label=New Scaffold
     promoter        274..708
                     /label=35Sen
     promoter        709..1171
                     /label=CmYLCV
     primer_bind     716..736
                     /label=35C-CmYLCV-1F
     primer_bind     1095..1114
                     /label=CmYLCV-EndF
     primer_bind     complement(1142..1164)
                     /label=LHC-265-2R
     promoter        1180..1267
                     /label=U6 (88-bp)
     primer_bind     1202..1223
                     /label=LHC-260-seqF
     tRNA            1275..1343
                     /label=tOsGly (2-bp deletion, CA)
     misc_feature    1345..1350
                     /label=B
     misc_feature    1371..1376
                     /label=B
     misc_feature    1377..1413
                     /label=tevopreQ1 (1-bp deletion, C)
     misc_feature    1414..1481
                     /label=HDV
     terminator      1490..1760
                     /label=HSP18.2t
     misc_feature    1755..1760
                     /label=Feature 19
     misc_feature    1773..3763
                     /label=Ubi promoter
     primer_bind     complement(1801..1824)
                     /label=LHC-265-1R
     primer_bind     1979..1997
                     /label=pH-ePPEplus-4F
     primer_bind     2725..2741
                     /label=pH-ePPEplus-5F
     primer_bind     3280..3296
                     /label=pH-ePPEplus-6F
     misc_feature    3746..3770
                     /label=Adapter
     CDS             3774..3800
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3807..3860
                     /codon_start=1
                     /label=bpSV40
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             3861..7961
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=nCas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     3881..3898
                     /label=pH-ePPEplus-7F
     primer_bind     4420..4436
                     /label=pH-ePPEplus-8F
     primer_bind     4915..4931
                     /label=pH-ePPEplus-9F
     primer_bind     5430..5447
                     /label=pH-ePPEplus-10F
     primer_bind     5970..5987
                     /label=pH-ePPEplus-11F
     primer_bind     6488..6507
                     /label=pH-eppeplus-12-3F
     primer_bind     7016..7032
                     /label=pH-ePPEplus-13F
     primer_bind     7539..7555
                     /label=pH-ePPEplus-15F
     misc_feature    7968..8015
                     /label=XTEN
     misc_feature    8016..8183
                     /label=NC
     CDS             8184..8204
                     /codon_start=1
                     /label=NLS
                     /translation="PKKKRKV"
     CDS             8211..8306
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             8313..9854
                     /codon_start=1
                     /label=MLV-delRNAseH
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     ADDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPL"
     misc_feature    8313..8381
                     /label=the first 23aa
     misc_feature    8382..8684
                     /label=24-124 aa Finger
     primer_bind     8532..8549
                     /label=pH-ePPEplus-16F
     misc_feature    8685..8777
                     /label=125-155aa PLAM
     misc_feature    8778..8897
                     /label=156-195aa Finger
     misc_feature    8898..9137
                     /label=196-275 aa PLAM
     primer_bind     9129..9148
                     /label=pH-ePPEplus-17F
     misc_feature    9138..9326
                     /label=276-338aa Thumb
     misc_feature    9327..9715
                     /label=339-468aa connection
     primer_bind     9586..9601
                     /label=pH-ePPEplus-18F
     primer_bind     complement(9794..9813)
                     /label=MLV-1500R
     CDS             9867..9920
                     /codon_start=1
                     /label=bpNLS
                     /translation="KRTADSQHSTPPKTKRKV"
     CDS             9930..9950
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     9950..9969
                     /label=pH-eppeplus-19-1F
     misc_feature    complement(9954..9978)
                     /label=AdapterR
     misc_feature    9960..10594
                     /label=E9ter
     primer_bind     10518..10539
                     /label=pH-ePPEplus-20F
     primer_bind     complement(10609..10625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10633..10649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10657..10687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10702..10723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    10854..11297
                     /label=pd35S(1X)
                     /label=pd35S
     promoter        10914..11265
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     11173..11192
                     /label=LHC-263-3F
     CDS             11310..11861
                     /codon_start=1
                     /label=bar
                     /note="phosphinothricin acetyltransferase from Streptomyces
                     hygroscopicus ATCC21705"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     complement(11555..11573)
                     /label=Bar-new1R
     polyA_signal    11868..12042
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(11925..11945)
                     /label=CaMVT-60R
     misc_feature    12119..12144
                     /label=LB
                     /note="LB"
     misc_feature    12120..12144
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12569..13360
                     /codon_start=1
                     /label=kan
                     /label=kan(R)
                     /note="Kan(R)"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13450..14038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      13654..13934
                     /direction=RIGHT
                     /label=pBR322 origin
     misc_feature    14224..14364
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    complement(14708..14902)
                     /label=pVS1 ori
     CDS             complement(14968..16041)
                     /codon_start=1
                     /label=RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16470..17099)
                     /codon_start=1
                     /label=staA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttatggagt caaagattca aatagaggac
      301 ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat
      361 gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
      421 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      481 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      541 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      601 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      661 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgattg gcagacatac
      721 tgtcccacaa atgaagatgg aatctgtaaa agaaaacgcg tgaaataatg cgtctgacaa
      781 aggttaggtc ggctgccttt aatcaatacc aaagtggtcc ctaccacgat ggaaaaactg
      841 tgcagtcggt ttggcttttt ctgacgaaca aataagattc gtggccgaca ggtgggggtc
      901 caccatgtga aggcatcttc agactccaat aatggagcaa tgacgtaagg gcttacgaaa
      961 taagtaaggg tagtttggga aatgtccact cacccgtcag tctataaata cttagcccct
     1021 ccctcattgt taagggagca aaatctcaga gagatagtcc tagagagaga aagagagcaa
     1081 gtagcctaga agtagtcaag gcggcgaagt attcaggcac gtggccagga agaagaaaag
     1141 ccaagacgac gaaaacaggt aagagctaag catctagata agttgaaaac aatcttcaaa
     1201 agtcccacat cgcttagata agaaaacgaa gctgagttta tatacagcta gagtcgaagt
     1261 agtgattgaa caaagcacca gtggtctagt ggtagaatag taccctgcca cggtacagac
     1321 ccgggttcga ttcccggctg gtgtgagacc ttcggggaat atgagcgtca ggtctccgcg
     1381 gttctatcta gttacgcgtt aaaccaacta gaaggccggc atggtcccag cctcctcgct
     1441 ggcgccggct gggcaacatg cttcggcatg gcgaatggga cttttttttg atatctccgg
     1501 ggctaattga atatgaagat gaagatgaaa tatttggtgt gtcaaataaa aagctggtgt
     1561 gcttaagttt gtgttttttt cttggcttgt tgtgttatga atttgtggct ttttctaata
     1621 ttaaatgaat gtaagatctc attataatga ataaacaaat gtttctataa tccattgtga
     1681 atgttttgtt ggatctcttc tgcagcatat aactactgta tgtgctatgg tatggactat
     1741 ggaatatgat taaagataag aagcttgcat gctgcagtgc agcgtgaccc ggtcgtgccc
     1801 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     1861 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     1921 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     1981 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     2041 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     2101 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     2161 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     2221 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     2281 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     2341 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     2401 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     2461 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     2521 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     2581 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc
     2641 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca
     2701 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc
     2761 cgctcgtcct cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt
     2821 agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc
     2881 gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac
     2941 ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc
     3001 gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc
     3061 aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg
     3121 tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattctg tttcaaacta
     3181 cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga
     3241 attgaagatg atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt
     3301 actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg
     3361 gcggtcgttc attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt
     3421 attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga
     3481 tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca
     3541 tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata
     3601 aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca
     3661 gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt
     3721 cttttgtcga tgctcaccct gttgtttggt gttacttctg cagccctagg atgcctgccg
     3781 ccaagagagt caagctcgat ggcggcaaga ggaccgccga tggcagcgag ttcgagtctc
     3841 ctaagaagaa gaggaaggtc gacaagaagt actcgatcgg cctcgatatt gggactaact
     3901 ctgttggctg ggccgtgatc accgacgagt acaaggtgcc ctcaaagaag ttcaaggtcc
     3961 tgggcaacac cgatcggcat tccatcaaga agaatctcat tggcgctctc ctgttcgaca
     4021 gcggcgagac ggctgaggct acgcggctca agcgcaccgc ccgcaggcgg tacacgcgca
     4081 ggaagaatcg catctgctac ctgcaggaga ttttctccaa cgagatggcg aaggttgacg
     4141 attctttctt ccacaggctg gaggagtcat tcctcgtgga ggaggataag aagcacgagc
     4201 ggcatccaat cttcggcaac attgtcgacg aggttgccta ccacgagaag taccctacga
     4261 tctaccatct gcggaagaag ctcgtggact ccacagataa ggcggacctc cgcctgatct
     4321 acctcgctct ggcccacatg attaagttca ggggccattt cctgatcgag ggggatctca
     4381 acccggacaa tagcgatgtt gacaagctgt tcatccagct cgtgcagacg tacaaccagc
     4441 tcttcgagga gaaccccatt aatgcgtcag gcgtcgacgc gaaggctatc ctgtccgcta
     4501 ggctctcgaa gtctcggaag ctcgagaacc tgatcgccca gctgccgggc gagaagaaga
     4561 acggcctgtt cgggaatctc attgcgctca gcctggggct cacgcccaac ttcaagtcga
     4621 atttcgatct cgctgaggac gccaagctgc agctctccaa ggacacatac gacgatgacc
     4681 tggataacct cctggcccag atcggcgatc agtacgcgga cctgttcctc gctgccaaga
     4741 atctgtcgga cgccatcctc ctgtctgata ttctcagggt gaacaccgag attacgaagg
     4801 ctccgctctc agcctccatg atcaagcgct acgacgagca ccatcaggat ctgaccctcc
     4861 tgaaggcgct ggtcaggcag cagctccccg agaagtacaa ggagatcttc ttcgatcagt
     4921 cgaagaacgg ctacgctggg tacattgacg gcggggcctc tcaggaggag ttctacaagt
     4981 tcatcaagcc gattctggag aagatggacg gcacggagga gctgctggtg aagctcaagc
     5041 gcgaggacct cctgaggaag cagcggacat tcgataacgg cagcatccca caccagattc
     5101 atctcgggga gctgcacgct atcctgagga ggcaggagga cttctaccct ttcctcaagg
     5161 ataaccgcga gaagatcgag aagattctga ctttcaggat cccgtactac gtcggcccac
     5221 tcgctagggg caactcccgc ttcgcttgga tgacccgcaa gtcagaggag acgatcacgc
     5281 cgtggaactt cgaggaggtg gtcgacaagg gcgctagcgc tcagtcgttc atcgagagga
     5341 tgacgaattt cgacaagaac ctgccaaatg agaaggtgct ccctaagcac tcgctcctgt
     5401 acgagtactt cacagtctac aacgagctga ctaaggtgaa gtatgtgacc gagggcatga
     5461 ggaagccggc tttcctgtct ggggagcaga agaaggccat cgtggacctc ctgttcaaga
     5521 ccaaccggaa ggtcacggtt aagcagctca aggaggacta cttcaagaag attgagtgct
     5581 tcgattcggt cgagatctct ggcgttgagg accgcttcaa cgcctccctg gggacctacc
     5641 acgatctcct gaagatcatt aaggataagg acttcctgga caacgaggag aatgaggata
     5701 tcctcgagga cattgtgctg acactcactc tgttcgagga ccgggagatg atcgaggagc
     5761 gcctgaagac ttacgcccat ctcttcgatg acaaggtcat gaagcagctc aagaggagga
     5821 ggtacaccgg ctgggggagg ctgagcagga agctcatcaa cggcattcgg gacaagcagt
     5881 ccgggaagac gatcctcgac ttcctgaaga gcgatggctt cgcgaaccgc aatttcatgc
     5941 agctgattca cgatgacagc ctcacattca aggaggatat ccagaaggct caggtgagcg
     6001 gccaggggga ctcgctgcac gagcatatcg cgaacctcgc tggctcgcca gctatcaaga
     6061 aggggattct gcagaccgtg aaggttgtgg acgagctggt gaaggtcatg ggcaggcaca
     6121 agcctgagaa catcgtcatt gagatggccc gggagaatca gaccacgcag aagggccaga
     6181 agaactcacg cgagaggatg aagaggatcg aggagggcat taaggagctg gggtcccaga
     6241 tcctcaagga gcacccggtg gagaacacgc agctgcagaa tgagaagctc tacctgtact
     6301 acctccagaa tggccgcgat atgtatgtgg accaggagct ggatattaac aggctcagcg
     6361 attacgacgt cgatgccatc gttccacagt cattcctgaa ggatgactcc attgacaaca
     6421 aggtcctcac caggtcggac aagaaccggg gcaagtctga taatgttcct tcagaggagg
     6481 tcgttaagaa gatgaagaac tactggcgcc agctcctgaa tgccaagctg atcacgcagc
     6541 ggaagttcga taacctcaca aaggctgaga ggggcgggct ctctgagctg gacaaggcgg
     6601 gcttcatcaa gaggcagctg gtcgagacac ggcagatcac taagcacgtt gcgcagattc
     6661 tcgactcacg gatgaacact aagtacgatg agaatgacaa gctgatccgc gaggtgaagg
     6721 tcatcaccct gaagtcaaag ctcgtctccg acttcaggaa ggatttccag ttctacaagg
     6781 ttcgggagat caacaattac caccatgccc atgacgcgta cctgaacgcg gtggtcggca
     6841 cagctctgat caagaagtac ccaaagctcg agagcgagtt cgtgtacggg gactacaagg
     6901 tttacgatgt gaggaagatg atcgccaagt cggagcagga gattggcaag gctaccgcca
     6961 agtacttctt ctactctaac attatgaatt tcttcaagac agagatcact ctggccaatg
     7021 gcgagatccg gaagcgcccc ctcatcgaga cgaacggcga gacgggggag atcgtgtggg
     7081 acaagggcag ggatttcgcg accgtcagga aggttctctc catgccacaa gtgaatatcg
     7141 tcaagaagac agaggtccag actggcgggt tctctaagga gtcaattctg cctaagcgga
     7201 acagcgacaa gctcatcgcc cgcaagaagg actgggatcc gaagaagtac ggcgggttcg
     7261 acagccccac tgtggcctac tcggtcctgg ttgtggcgaa ggttgagaag ggcaagtcca
     7321 agaagctcaa gagcgtgaag gagctgctgg ggatcacgat tatggagcgc tccagcttcg
     7381 agaagaaccc gatcgatttc ctggaggcga agggctacaa ggaggtgaag aaggacctga
     7441 tcattaagct ccccaagtac tcactcttcg agctggagaa cggcaggaag cggatgctgg
     7501 cttccgctgg cgagctgcag aaggggaacg agctggctct gccgtccaag tatgtgaact
     7561 tcctctacct ggcctcccac tacgagaagc tcaagggcag ccccgaggac aacgagcaga
     7621 agcagctgtt cgtcgagcag cacaagcatt acctcgacga gatcattgag cagatttccg
     7681 agttctccaa gcgcgtgatc ctggccgacg cgaatctgga taaggtcctc tccgcgtaca
     7741 acaagcaccg cgacaagcca atcagggagc aggctgagaa tatcattcat ctcttcaccc
     7801 tgacgaacct cggcgcccct gctgctttca agtacttcga cacaactatc gatcgcaaga
     7861 ggtacacaag cactaaggag gtcctggacg cgaccctcat ccaccagtcg attaccggcc
     7921 tctacgagac gcgcatcgac ctgtctcagc tcgggggcga cgaattctcc gggagcgaga
     7981 cgccaggcac ctccgagtcg gccaccccag aatctgccac agtggtgtcc ggccaaaagc
     8041 aggaccgcca gggcggagaa cgcagaaggt cccagctcga tagggatcag tgtgcctact
     8101 gcaaggagaa gggccactgg gccaaagact gcccgaaaaa gccgcgcggc ccacgcggcc
     8161 caaggccaca aacatccctc cttccaaaga agaagcggaa ggtggagctc agcggaggat
     8221 cttccggagg atctagcggc tccgagacac caggaacatc cgaaagcgct acaccagaat
     8281 ctagcggagg ctcttccgga ggatctaggc ctaccctcaa catcgaggat gagtatcgcc
     8341 tccacgaaac ctccaaagaa ccggacgtgt ccctcggcag cacatggctc agcgacttcc
     8401 cacaagcgtg ggccgaaacc ggcggcatgg gcctcgccgt ccgccaagcc ccactcatta
     8461 tcccgctgaa ggcgacctcc acaccggtgt ccatcaagca gtacccgatg agccaagagg
     8521 cgaggctcgg gattaagccg cacattcagc gcctcctcga tcaaggcatt ctcgtgccgt
     8581 gccaatcccc gtggaataca ccactcctcc cggtcaaaaa gccgggcacc aacgactatc
     8641 gcccggtcca agatctccgc gaggtcaaca agcgcgtgga agacatccac ccgaccgtcc
     8701 cgaacccgta taatctgctc tccgggctcc caccatccca ccagtggtat acagtgctgg
     8761 acctcaaaga cgccttcttc tgtctccgcc tccacccaac aagccagccg ctcttcgcct
     8821 tcgagtggcg cgacccggag atgggcatct ccggccaact gacatggaca cgcctcccgc
     8881 aaggcttcaa gaacagcccg acactcttca acgaggcgct ccatagggac ctcgcggatt
     8941 ttcgcatcca gcatccggac ctcatcctcc tccagtatgc ggatgatctc ctcctcgccg
     9001 cgacctccga gctggattgt caacaaggca cacgcgcgct cctccaaaca ctcgggaacc
     9061 tcggctatcg cgcgtccgcg aaaaaggccc aaatctgcca gaagcaagtg aagtacctcg
     9121 ggtatctgct caaggaaggc caacgctggc tcaccgaagc gcgcaaagaa acagtgatgg
     9181 ggcaaccgac accgaaaaca ccacgccagc tgcgcgagtt tctcggcaaa gccggcttct
     9241 gtcgcctctt catcccgggc tttgccgaga tggccgcgcc actctaccca ctcaccaagc
     9301 cgggcacact gtttaactgg gggccggatc agcagaaagc ctaccaagag atcaaacaag
     9361 cgctcctcac cgccccagcg ctcgggctcc cagatctcac aaagccgttc gagctgttcg
     9421 tcgatgagaa gcaaggctac gcgaagggcg tgctcacaca gaagctcggc ccgtggagga
     9481 ggccagtggc ctatctctcc aaaaaactcg atccagtggc cgccggctgg ccaccgtgtc
     9541 tgcgcatggt cgccgcgatt gccgtgctca caaaggatgc cggcaaactc acaatgggcc
     9601 agccgctggt gatcctcgcg ccacatgccg tggaagccct cgtcaaacag ccgccggata
     9661 ggtggctctc caatgcgcgc atgacccatt accaagcgct cctcctcgac accgatcgcg
     9721 tccagttcgg cccagtggtc gccctcaatc cggcgacact gctgccactc ccagaggagg
     9781 gcctccaaca caactgtctg gatattctcg cggaagcgca tggcacaagg ccagacctca
     9841 cagatcaacc gctcagcggc ggcagcaaga ggaccgccga ctcccagcac tcgaccccgc
     9901 cgaagaccaa gaggaaggtg gagttcgagc cgaagaagaa gaggaaggtc tgagagctca
     9961 gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca
    10021 ttgcgcacac accagaatcc tactgagttt gagtattatg gcattgggaa aactgttttt
    10081 cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa
    10141 ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct
    10201 caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga
    10261 gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa
    10321 gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac taggcaacaa
    10381 atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg
    10441 ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc agattctaat
    10501 cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa aatatttttt
    10561 aatgcatttt atgacttgcc aattgattga caacgaattc gtaatcatgt catagctgtt
    10621 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    10681 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    10741 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10801 ggggagaggc ggtttgcgta ttggctagag cagcttgcca acatggtgga gcacgacact
    10861 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
    10921 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
    10981 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
    11041 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
    11101 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
    11161 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
    11221 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc
    11281 tctacaaatc tatctctctc gagtctacca tgagcccaga acgacgcccg gccgacatcc
    11341 gccgtgccac cgaggcggac atgccggcgg tctgcaccat cgtcaaccac tacatcgaga
    11401 caagcacggt caacttccgt accgagccgc aggaaccgca ggagtggacg gacgacctcg
    11461 tccgtctgcg ggagcgctat ccctggctcg tcgccgaggt ggacggcgag gtcgccggca
    11521 tcgcctacgc gggcccctgg aaggcacgca acgcctacga ctggacggcc gagtcgaccg
    11581 tgtacgtctc cccccgccac cagcggacgg gactgggctc cacgctctac acccacctgc
    11641 tgaagtccct ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg ctgcccaacg
    11701 acccgagcgt gcgcatgcac gaggcgctcg gatatgcccc ccgcggcatg ctgcgggcgg
    11761 ccggcttcaa gcacgggaac tggcatgacg tgggtttctg gcagctggac ttcagcctgc
    11821 cggtaccgcc ccgtccggtc ctgcccgtca ccgagatttg actcgagttt ctccataata
    11881 atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt
    11941 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
    12001 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat taattcggcg
    12061 ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
    12121 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
    12181 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
    12241 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
    12301 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
    12361 agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat actatgttat
    12421 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
    12481 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
    12541 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
    12601 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
    12661 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
    12721 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
    12781 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
    12841 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
    12901 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
    12961 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
    13021 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
    13081 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
    13141 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
    13201 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
    13261 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
    13321 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag aatgcatgac
    13381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    13441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    13501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    13561 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    13621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    13681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    13741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    13801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    13861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    13921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    13981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    14041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    14101 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    14161 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    14221 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
    14281 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
    14341 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
    14401 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
    14461 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
    14521 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
    14581 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
    14641 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
    14701 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
    14761 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
    14821 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
    14881 cccaatgtac gtgctatcca caggaaacag accttttcga cctttttccc ctgctagggc
    14941 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
    15001 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
    15061 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
    15121 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
    15181 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
    15241 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
    15301 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
    15361 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
    15421 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
    15481 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
    15541 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
    15601 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
    15661 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
    15721 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
    15781 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
    15841 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
    15901 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
    15961 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
    16021 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
    16081 ggccggcagg caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
    16141 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
    16201 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
    16261 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
    16321 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
    16381 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
    16441 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
    16501 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
    16561 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
    16621 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
    16681 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
    16741 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
    16801 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
    16861 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
    16921 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
    16981 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
    17041 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
    17101 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
    17161 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
    17221 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
    17281 gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct
    17341 ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca
    17401 tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc
    17461 gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc
    17521 aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt
    17581 gcggccttcc tgttcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg
    17641 gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc
    17701 gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc
    17761 ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc
    17821 ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct
    17881 ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg
    17941 gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag
    18001 ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag
    18061 gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta
    18121 ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg
    18181 tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca
    18241 gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga
    18301 accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt
    18361 aaatatccga ttattctaa
//