pZH509-FtsZ-mBiyu

LOCUS       pZH509-FtsZ-mBiyu             5436 bp DNA     circular SYN 13-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 28, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..262
                     /locus_tag="pLtetO-1"
                     /label=pLtetO-1 promoter
     protein_bind    189..207
                     /locus_tag="TetO2"
     -35_signal      208..213
                     /locus_tag="-33"
     protein_bind    214..232
                     /locus_tag="TetO2(1)"
     -10_signal      231..236
                     /locus_tag="-10"
     misc_feature    243
                     /locus_tag="pLtetO-1 +1"
     RBS             272..283
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    292..1440
                     /label=FtsZ
     CDS             1441..1485
                     /codon_start=1
                     /label=linker0
                     /translation="GGGGSPAPAPGGGGS"
     CDS             1486..2229
                     /codon_start=1
                     /label=mBiyu
                     /translation="VSKGEEDNMASYGALLFREKIPYVVEMEGDVEGMKFSVRGKGHGD
                     ANTGKIEASFICTTGELPVPWSSILTTVTYGAQCFAKYPNDIKDYLKSTMPEGYVQERT
                     ITFENDGIYKTRAEVTYEKGSVYNRVTLNGSGFKKGGNILGKKLEFNFNASRTYVLPDV
                     ENNGIKCYINIRHNVIGGGQIRAEHQQLNTPLGGGPVDIPHYHAIQTHTILSKDPKETR
                     DHMNVVEVFRAIDCKTAYAGMDELYK"
     RBS             2239..2244
                     /locus_tag="RBS"
     CDS             2253..2876
                     /locus_tag="TetR tn10"
                     /label=TetR tn10
     terminator      2921..2967
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2924..2967
                     /locus_tag="rrnB_T1_terminator"
                     /label=rrnB_T1_terminator
     primer_bind     complement(2986..3002)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3010..3026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3034..3064)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3079..3100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3373..4216
                     /locus_tag="p15A_origin"
     rep_origin      complement(3640..4185)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     gene            4358..5436
                     /locus_tag="AmpR"
                     /label=AmpR
     CDS             complement(4376..5236)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5237..5341)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      241 acatcagcag gacgcactga ccgaattcat taaagaggag aaaggtaccg catgtttgaa
      301 ccaatggaac ttaccaatga cgcggtgatt aaagtcatcg gcgtcggcgg cggcggcggt
      361 aatgctgttg aacacatggt gcgcgagcgc attgaaggtg ttgaattctt cgcggtaaat
      421 accgatgcac aagcgctgcg taaaacagcg gttggacaga cgattcaaat cggtagcggt
      481 atcaccaaag gactgggcgc tggcgctaat ccagaagttg gccgcaatgc ggctgatgag
      541 gatcgcgatg cattgcgtgc ggcgctggaa ggtgcagaca tggtctttat tgctgcgggt
      601 atgggtggtg gtaccggtac aggtgcagca ccagtcgtcg ctgaagtggc aaaagatttg
      661 ggtatcctga ccgttgctgt cgtcactaag cctttcaact ttgaaggcaa gaagcgtatg
      721 gcattcgcgg agcaggggat cactgaactg tccaagcatg tggactctct gatcactatc
      781 ccgaacgaca aactgctgaa agttctgggc cgcggtatct ccctgctgga tgcgtttggc
      841 gcagcgaacg atgtactgaa aggcgctgtg caaggtatcg ctgaactgat tactcgtccg
      901 ggtttgatga acgtggactt tgcagacgta cgcaccgtaa tgtctgagat gggctacgca
      961 atgatgggtt ctggcgtggc gagcggtgaa gaccgtgcgg aagaagctgc tgaaatggct
     1021 atctcttctc cgctgctgga agatatcgac ctgtctggcg cgcgcggcgt gctggttaac
     1081 atcacggcgg gcttcgatct gcgtctggat gagttcgaaa cggtaggtaa caccatccgt
     1141 gcatttgctt ccgacaacgc gactgtggtt atcggtactt ctcttgaccc ggatatgaat
     1201 gacgagctgc gcgtaaccgt tgttgcgaca ggtatcggca tggacaaacg tcctgaaatc
     1261 actctggtga ccaataagca ggttcagcag ccagtgatgg atcgctacca gcagcatggg
     1321 atggctccgc tgacccagga gcagaagccg gttgctaaag tcgtgaatga caatgcgccg
     1381 caaactgcga aagagccgga ttatctggat atcccagcat tcctgcgtaa gcaagctgat
     1441 ggtggaggag ggagcccggc ccctgctccc ggaggcgggg gcagcgtgag caaaggcgaa
     1501 gaagataaca tggcgagtta cggagcactt ttgttcagag aaaaaatccc ctatgttgtt
     1561 gagatggaag gtgatgttga gggaatgaag ttttcagtaa gaggtaaagg acatggtgat
     1621 gctaacactg gtaaaattga agccagtttc atctgcacaa ctggtgaact accagtacct
     1681 tggtcgagca ttctaacaac tgtcacatac ggtgcacagt gttttgcaaa atatccaaat
     1741 gatatcaaag actacctaaa aagtactatg ccagaaggtt acgttcaaga gcgaacaatc
     1801 acttttgaaa acgacggcat ttataagaca cgcgctgaag tcacgtacga gaaaggatca
     1861 gtttataacc gagttacgtt gaacggttca ggatttaaaa aaggcggtaa catcttgggc
     1921 aaaaaattgg aatttaactt caacgcttcg cgcacttatg ttcttccaga tgtagaaaat
     1981 aatggaatca aatgctacat caacattcgt cataacgtaa ttggaggagg acaaatacga
     2041 gctgagcacc aacaattaaa tactcctctt ggcggtggac cagtcgacat tccacactac
     2101 catgcgatac aaacgcacac aatactttct aaagatccca aagaaacaag agatcatatg
     2161 aacgtagtag aggtgttcag agctatcgat tgcaaaaccg catatgcagg catggatgaa
     2221 ctgtataaat aatctagcag gaggatttca ccatgtctag attagataaa agtaaagtga
     2281 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
     2341 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
     2401 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
     2461 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
     2521 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
     2581 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg
     2641 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
     2701 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
     2761 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
     2821 ttgaattgat catctgcgga ttagaaaaac aacttaaatg tgaaagtggg tcttaataac
     2881 tgcagcccgg gggatcccat ggtacgcgtg ctagaggcat caaataaaac gaaaggctca
     2941 gtcgaaagac tgggcctttc gttttataag cttggcgtaa tcatggtcat agctgtttcc
     3001 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3061 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3121 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3181 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3241 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3301 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3361 ccgtaaaaag gcgcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt
     3421 gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata
     3481 tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     3541 tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga
     3601 tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc
     3661 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
     3721 aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg
     3781 gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca
     3841 gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
     3901 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag
     3961 caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc
     4021 ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct
     4081 cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt
     4141 tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt
     4201 aatcagataa aatattgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4261 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4321 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4381 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4441 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4501 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4561 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4621 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4681 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4741 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4801 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4861 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4921 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4981 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5041 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5101 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5161 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5221 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5281 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5341 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     5401 cctataaaaa taggcgtatc acgaggccct ttcgtc
//