pZH509-ClpP-mBiyu

LOCUS       pZH509-ClpP-mBiyu          4878 bp DNA     circular SYN 13-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 28, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..262
                     /locus_tag="pLtetO-1"
                     /label=pLtetO-1 promoter
     protein_bind    189..207
                     /locus_tag="TetO2"
     -35_signal      208..213
                     /locus_tag="-33"
     protein_bind    214..232
                     /locus_tag="TetO2(1)"
     -10_signal      231..236
                     /locus_tag="-10"
     misc_feature    243
                     /locus_tag="pLtetO-1 +1"
     RBS             272..283
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             292..912
                     /codon_start=1
                     /label=ClpP
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIFL
                     TGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVSTI
                     CMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVKGRM
                     NELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     CDS             928..1671
                     /codon_start=1
                     /label=mBiyu
                     /translation="VSKGEEDNMASYGALLFREKIPYVVEMEGDVEGMKFSVRGKGHGD
                     ANTGKIEASFICTTGELPVPWSSILTTVTYGAQCFAKYPNDIKDYLKSTMPEGYVQERT
                     ITFENDGIYKTRAEVTYEKGSVYNRVTLNGSGFKKGGNILGKKLEFNFNASRTYVLPDV
                     ENNGIKCYINIRHNVIGGGQIRAEHQQLNTPLGGGPVDIPHYHAIQTHTILSKDPKETR
                     DHMNVVEVFRAIDCKTAYAGMDELYK"
     RBS             1681..1686
                     /locus_tag="RBS"
     CDS             1695..2318
                     /locus_tag="TetR tn10"
                     /label=TetR tn10
     terminator      2363..2409
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2366..2409
                     /locus_tag="rrnB_T1_terminator"
                     /label=rrnB_T1_terminator
     primer_bind     complement(2428..2444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2452..2468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2476..2506)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2521..2542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2815..3658
                     /locus_tag="p15A_origin"
     rep_origin      complement(3082..3627)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     gene            3800..4878
                     /locus_tag="AmpR"
                     /label=AmpR
     CDS             complement(3818..4678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4679..4783)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      241 acatcagcag gacgcactga ccgaattcat taaagaggag aaaggtaccg catgtcatac
      301 agcggcgaac gagataactt tgcaccccat atggcgctgg tgccgatggt cattgaacag
      361 acctcacgcg gtgagcgctc ttttgatatc tattctcgtc tacttaagga acgcgtcatt
      421 tttctgactg gccaggttga agaccacatg gctaacctga ttgtggcgca gatgctgttc
      481 ctggaagcgg aaaacccaga aaaagatatc tatctgtaca ttaactcccc aggcggggtg
      541 atcactgccg ggatgtctat ctatgacacc atgcagttta tcaagcctga tgtcagcacc
      601 atctgtatgg gccaggcggc ctcgatgggc gctttcttgc tgaccgcagg ggcaaaaggt
      661 aaacgttttt gcctgccgaa ttcgcgcgtg atgattcacc aaccgttggg cggctaccag
      721 ggccaggcga ccgatatcga aattcatgcc cgtgaaattc tgaaagttaa agggcgcatg
      781 aatgaactta tggcgcttca tacgggtcaa tcattagaac agattgaacg tgataccgag
      841 cgcgatcgct tcctttccgc ccctgaagcg gtggaatacg gtctggtcga ttcgattctg
      901 acccatcgta atagcggtgg aggaggggtg agcaaaggcg aagaagataa catggcgagt
      961 tacggagcac ttttgttcag agaaaaaatc ccctatgttg ttgagatgga aggtgatgtt
     1021 gagggaatga agttttcagt aagaggtaaa ggacatggtg atgctaacac tggtaaaatt
     1081 gaagccagtt tcatctgcac aactggtgaa ctaccagtac cttggtcgag cattctaaca
     1141 actgtcacat acggtgcaca gtgttttgca aaatatccaa atgatatcaa agactaccta
     1201 aaaagtacta tgccagaagg ttacgttcaa gagcgaacaa tcacttttga aaacgacggc
     1261 atttataaga cacgcgctga agtcacgtac gagaaaggat cagtttataa ccgagttacg
     1321 ttgaacggtt caggatttaa aaaaggcggt aacatcttgg gcaaaaaatt ggaatttaac
     1381 ttcaacgctt cgcgcactta tgttcttcca gatgtagaaa ataatggaat caaatgctac
     1441 atcaacattc gtcataacgt aattggagga ggacaaatac gagctgagca ccaacaatta
     1501 aatactcctc ttggcggtgg accagtcgac attccacact accatgcgat acaaacgcac
     1561 acaatacttt ctaaagatcc caaagaaaca agagatcata tgaacgtagt agaggtgttc
     1621 agagctatcg attgcaaaac cgcatatgca ggcatggatg aactgtataa ataatctagc
     1681 aggaggattt caccatgtct agattagata aaagtaaagt gattaacagc gcattagagc
     1741 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
     1801 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
     1861 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
     1921 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
     1981 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
     2041 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
     2101 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
     2161 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
     2221 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatctgcg
     2281 gattagaaaa acaacttaaa tgtgaaagtg ggtcttaata actgcagccc gggggatccc
     2341 atggtacgcg tgctagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     2401 tcgttttata agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     2461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggcgcgcta
     2821 gcggagtgta tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat
     2881 gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt
     2941 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg
     3001 cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga
     3061 gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat
     3121 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3181 ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc
     3241 gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc
     3301 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg
     3361 gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca
     3421 ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa
     3481 aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta
     3541 gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag
     3601 attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatattga
     3661 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3721 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3781 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3841 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3901 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3961 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4021 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4081 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4141 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4201 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4261 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4321 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4381 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4441 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4501 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4561 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4621 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4681 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4741 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4801 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4861 tcacgaggcc ctttcgtc
//