| 质粒名称: | pYK481 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000823 |
| 产品用途: | Mammalian PhCMV-driven constitutive TOM201-70-GNCR-mCherry expression vector (PhCMV-TOM201-70-GNCR-mCherry-pA) |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: |
TOM201-70-GNCR-mCherry
|
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK481
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK481载体质粒基本信息
pYK481质粒图谱载体图谱和pYK481载体序列质粒序列多克隆位点信息
pYK481质粒载体简介
pYK481质粒序列载体序列
LOCUS pYK481 7083 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7083)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7083
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..899
/label=oYF0019
primer_bind 881..912
/label=oYK31-mCherry-NS3a-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
misc_feature 907..1120
/label=TOM_EWSR1
CDS 907..1116
/codon_start=1
/label=TOM20_1-70
/translation="MVGRNSAIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQ
KLAKERAGLSKLPDLKDAEAVQKFF"
primer_bind complement(1096..1136)
/label=oYK481_TOM_R
primer_bind 1116..1153
/label=oYF005
primer_bind 1116..1147
/label=oYF0019
primer_bind 1116..1138
/label=oYK490_GNCR_F
primer_bind 1117..1138
/label=oYK481_GNCR_F
CDS 1120..1818
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 1120..1153
/label=oYF0011
primer_bind 1120..1144
/label=oLZ146 GNCR1-N F
primer_bind 1120..1144
/label=oSCL762
primer_bind 1786..1798
/label=oYK03-02-F
primer_bind 1786..1797
/label=oYK31-mCherry-NS3a-F
primer_bind complement(1797..1854)
/label=oYF0020
primer_bind complement(1797..1848)
/label=oYF006
primer_bind complement(1797..1819)
/label=oYF0012
primer_bind complement(1806..1825)
/label=oYK465_GNCR1_R
primer_bind 1816..1838
/label=oSCL1129
primer_bind 1816..1836
/label=oLZ147 NS3a F
primer_bind 1816..1835
/label=oYF0033
primer_bind complement(1818..1844)
/label=oDJ171-4
primer_bind 1818..1838
/label=oLZ048 NSP3-F
primer_bind 1818..1835
/label=oYK419_GS_F
primer_bind 1819..1896
/label=oDJ171-3
primer_bind 1819..1848
/label=oSCL388
primer_bind 1819..1839
/label=oYK451_NLS_F
primer_bind 1819..1834
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1820..1868)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(1826..1844)
/label=Oscl18-4
primer_bind complement(1829..1849)
/label=oSCL1119
primer_bind complement(1829..1848)
/label=oSCL075
primer_bind complement(1829..1848)
/label=oSCL075a
primer_bind complement(1829..1848)
/label=oSCL075`b
primer_bind complement(1832..1848)
/label=oYK472_NLS_R
primer_bind 1833..1853
/label=oLZ146 GNCR1-N F
primer_bind complement(1833..1849)
/label=oYK92_L7Ae_R
misc_feature 1835..1853
/label=linker
primer_bind 1835..1849
/label=oYK490_GNCR_F
primer_bind complement(1837..1892)
/label=oYK75_NLS_mcp_R
primer_bind 1839..1891
/label=oYK03-02-F
primer_bind complement(1846..1869)
/label=oYK421_GNCR3_R
primer_bind 1850..1887
/label=oYK421_mCherry_F
misc_feature 1854
/label=linker
misc_feature 1855..1869
/label=NSP3
primer_bind 1870..1893
/label=oXQ135
primer_bind 1870..1893
/label=oXQ174
primer_bind 1870..1893
/label=omz_mCherry_F
primer_bind 1870..1890
/label=oYK31-mCherry-NS3a-F
CDS 1873..2577
/codon_start=1
/label=mCherry
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind 1873..1899
/label=omz_linker_mCherry_F
primer_bind 1914..1932
primer_bind 1914..1932
/label=DDL7AeF
/note="KpnI"
primer_bind 1914..1932
/label=L7Ae FLP
primer_bind complement(2185..2196)
/label=oSCL011
primer_bind complement(2185..2196)
/label=omz_Coh2_R
primer_bind complement(2185..2196)
/label=omz_Coh2_His_R
primer_bind 2317..2326
/label=oYK92_L7Ae_R
primer_bind 2318..2334
/label=mCherry_F测序
primer_bind 2386..2399
/label=oYK451_NLS_F
primer_bind complement(2557..2621)
/label=oXQ161
primer_bind complement(2557..2600)
/label=oYK05-mcherry-R
primer_bind complement(2557..2585)
/label=oXQ136
primer_bind complement(2557..2579)
/label=oYK56-mCherry-R
primer_bind complement(2557..2579)
/label=oYK58-mCherry-R
primer_bind complement(2557..2577)
/label=oDJ171-4
primer_bind complement(2557..2577)
/label=omz_mCherry_His_R
primer_bind complement(2557..2577)
/label=oYK316_mCherry_CAAX_R
primer_bind complement(2559..2577)
/label=omz_mCherry_linker_R
primer_bind complement(2565..2601)
/label=oYK431_NES2_R
primer_bind complement(2578..2603)
/label=oYK472_NLS_R
primer_bind complement(2581..2603)
/label=oYK57-MCP-mCherry-R
terminator 2677..2904
/label=bGH PA
misc_feature 2950..3378
/label=f1 origin
misc_feature 3383..3726
/label=SV40 early promoter and origin
misc_feature 3572..3649
/label=SV40 ORI
CDS 3791..4582
/label=Kan/neoR
misc_feature 4756..4886
/label=SV40 early polyadenylation signal
primer_bind complement(4922..4942)
/label=M13-rev
misc_binding complement(4948..4970)
/label=LacO
promoter complement(4975..5004)
/label=lac
misc_feature complement(5269..5942)
/label=pUC origin
CDS complement(6090..6749)
/label=AmpR
promoter complement(6989..7017)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gccaccatgg tggggaggaa cagcgccatc gccgccggag tgtgcggcgc tctgttcatc
961 ggctactgca tctacttcga cagaaaaaga agaagcgacc ccaacttcaa gaaccggctg
1021 agagagagaa gaaaaaagca gaagctggcc aaggagagag ccggcctgag caagctgccc
1081 gacctgaagg acgccgaggc cgtgcagaag ttcttcatgg acatcgagaa gctgtgcaag
1141 aaggcggaag aggaggccaa ggaggcccag gagaaggccg acgagctgag acaacgacac
1201 cccgattcgc aggcggcgga agacgcggag gatctcgcaa atctggctgt cgccgccgtg
1261 ctgaccgctt gtctgctggc ccaggagcat cccaacgccg atatcgctaa gctgtgcatc
1321 aaggccgcga gcgaagctgc ggaggccgcg tcaaaagccg ccgagctggc ccaacggcac
1381 ccagacagtc aagcagccag agatgctatc aaactcgcct ctcaagccgc ccgcgccgtg
1441 atcctggcca tcatgctcgc cgcagagaat cccaacgctg atattgcaaa gctatgtatt
1501 aaggcggcca gcgaagccgc agaggctgcc tcgaaagctg cggaacttgc acagcggcac
1561 cccgactccc aggccgcccg agatgcaatt aaactggcca gtcaggccgc tgaggcggtg
1621 gagagagcca tctggctggc cgccgagaac cccaatgccg atatcgccaa aaaatgcatc
1681 aaagcggcct ccgaagcagc cgaggaggcc tctaaagctg ccgaagaggc tcaaagacac
1741 ccagacagcc agaaggcacg cgacgagatc aaggaggcct ctcagaaggc cgaggaggtg
1801 aaggagagat gcaagagcgc cagcggaagt ggcggaggag gcgacgtcgc tagcatgctc
1861 aagatggaga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
1921 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
1981 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
2041 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
2101 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
2161 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
2221 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
2281 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
2341 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
2401 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
2461 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
2521 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
2581 ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg
2641 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
2701 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
2761 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
2821 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
2881 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
2941 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
3001 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
3061 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
3121 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
3181 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
3241 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
3301 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
3361 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
3421 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
3481 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
3541 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
3601 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
3661 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
3721 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
3781 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
3841 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
3901 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
3961 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
4021 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
4081 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
4141 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
4201 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
4261 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
4321 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
4381 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
4441 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
4501 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
4561 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
4621 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
4681 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
4741 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
4801 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
4861 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
4921 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
4981 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
5041 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
5101 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
5161 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
5221 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
5281 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
5341 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
5401 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
5461 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
5521 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
5581 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
5641 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
5701 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
5761 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
5821 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
5881 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
5941 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
6001 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
6061 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
6121 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
6181 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
6241 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
6301 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
6361 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
6421 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
6481 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
6541 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
6601 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
6661 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
6721 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
6781 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
6841 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
6901 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
6961 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
7021 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
7081 gtc
//
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pYK481质粒载体应用举例
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