pET30a-RFP-TS-Tn5-GT

LOCUS       pET30a-RFP-TS-Tn5-GT             9006 bp DNA     circular SYN 20-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(560..1375)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        4988..5006
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5007..5031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5046..5068
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    5076..5771
                     /label=mScarlet
     misc_feature    5772..6617
                     /label=Ts
     CDS             6618..8042
                     /codon_start=1
                     /product="transposase from the bacterial Tn5 transposon 
                     (Reznikoff, 1993)"
                     /label=Tn5 transposase
                     /translation="ITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKSI
                     TISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYRH
                     QVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESGK
                     WLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGLY
                     LYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNAV
                     LAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAERQ
                     RMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTPD
                     ECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQSK
                     LDGFLAAKDLMAQGIKI"
     misc_feature    8043..8048
                     /label=GT linker
     CDS             8049..8642
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             8673..8828
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             8850..8867
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      8934..8981
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatcga tctcgatccc
     4981 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
     5041 ataattttgt ttaactttaa gaaggagata tacatatggt ttctaaaggt gaagcggtta
     5101 tcaaagaatt catgcgtttc aaagttcaca tggaaggttc tatgaacggt cacgaattcg
     5161 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgcg aaactgaaag
     5221 ttaccaaagg tggtccgctg ccgttctctt gggacatcct gtctccgcag ttcatgtacg
     5281 gttctcgtgc gttcatcaaa cacccggcgg acatcccgga ctactacaaa cagtctttcc
     5341 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgcg gttaccgtta
     5401 cccaggacac ctctctggaa gacggtaccc tgatctacaa agttaaactg cgtggtacca
     5461 acttcccgcc ggacggtccg gttatgcaga aaaaaaccat gggttgggaa gcgtctaccg
     5521 aacgtctgta cccggaagac ggtgttctga aaggtgacat caaaatggcg ctgcgtctga
     5581 aagacggtgg tcgttacctg gcggacttca aaaccaccta caaagcgaaa aaaccggttc
     5641 agatgccggg tgcgtacaac gttgaccgta aactggacat cacctctcac aacgaagact
     5701 acaccgttgt tgaacagtac gaacgttctg aaggtcgtca ctctaccggt ggtatggacg
     5761 aactgtacaa agctgaaatt accgcatccc tggtaaaaga gctgcgtgag cgtactggcg
     5821 caggcatgat ggattgcaaa aaagcactga ctgaagctaa cggcgacatc gagctggcaa
     5881 tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa aaaagcaggc aacgttgctg
     5941 ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg catcattctg gaagttaact
     6001 gccagactga cttcgttgca aaagacgctg gtttccaggc gttcgcagac aaagttctgg
     6061 acgcagctgt tgctggcaaa atcactgacg ttgaagttct gaaagcacag ttcgaagaag
     6121 aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa cattcgccgc gttgctgcgc
     6181 tggaaggcga cgttctgggt tcttatcagc acggtgcgcg tatcggcgtt ctggttgctg
     6241 ctaaaggcgc tgacgaagag ctggttaaac acatcgctat gcacgttgct gcaagcaagc
     6301 cagaattcat caaaccggaa gacgtatccg ctgaagtggt agaaaaagaa taccaggtac
     6361 agctggatat cgcgatgcag tctggtaagc cgaaagaaat cgcagagaaa atggttgaag
     6421 gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg tcagccgttc gttatggaac
     6481 caagcaaaac tgttggtcag ctgctgaaag agcataacgc tgaagtgact ggcttcatcc
     6541 gcttcgaagt gggtgaaggc atcgagaaag ttgagactga ctttgcagca gaagttgctg
     6601 cgatgtccaa gcagtctatt accagtgcac tgcatcgtgc ggcggattgg gcgaaaagcg
     6661 tgttttctag tgctgcgctg ggtgatccgc gtcgtaccgc gcgtctggtg aatgttgcgg
     6721 cgcaactggc caaatatagc ggcaaaagca ttaccattag cagcgaaggc agcaaagcca
     6781 tgcaggaagg cgcgtatcgt tttattcgta atccgaacgt gagcgcggaa gcgattcgta
     6841 aagcgggtgc catgcagacc gtgaaactgg cccaggaatt tccggaactg ctggcaattg
     6901 aagataccac ctctctgagc tatcgtcatc aggtggcgga agaactgggc aaactgggta
     6961 gcattcagga taaaagccgt ggttggtggg tgcatagcgt gctgctgctg gaagcgacca
     7021 cctttcgtac cgtgggcctg ctgcatcaag aatggtggat gcgtccggat gatccggcgg
     7081 atgcggatga aaaagaaagc ggcaaatggc tggccgctgc tgcaacttcg cgtctgagaa
     7141 tgggcagcat gatgagcaac gtgattgcgg tgtgcgatcg tgaagcggat attcatgcgt
     7201 atctgcaaga taaactggcc cataacgaac gttttgtggt gcgtagcaaa catccgcgta
     7261 aagatgtgga aagcggcctg tatctgtatg atcacctgaa aaaccagccg gaactgggcg
     7321 gctatcagat tagcattccg cagaaaggcg tggtggataa acgtggcaaa cgtaaaaacc
     7381 gtccggcgcg taaagcgagc ctgagcctgc gtagcggccg tattaccctg aaacagggca
     7441 acattaccct gaacgcggtg ctggccgaag aaattaatcc gccgaaaggc gaaaccccgc
     7501 tgaaatggct gctgctgacc agcgagccgg tggaaagtct ggcccaagcg ctgcgtgtga
     7561 ttgatattta tacccatcgt tggcgcattg aagaatttca caaagcgtgg aaaacgggtg
     7621 cgggtgcgga acgtcagcgt atggaagaac cggataacct ggaacgtatg gtgagcattc
     7681 tgagctttgt ggcggtgcgt ctgctgcaac tgcgtgaatc ttttactccg ccgcaagcac
     7741 tgcgtgcgca gggcctgctg aaagaagcgg aacacgttga aagccagagc gcggaaaccg
     7801 tgctgacccc ggatgaatgc caactgctgg gctatctgga taaaggcaaa cgcaaacgca
     7861 aagaaaaagc gggcagcctg caatgggcgt atatggcgat tgcgcgtctg ggcggcttta
     7921 tggatagcaa acgtaccggc attgcgagct ggggtgcgct gtgggaaggt tgggaagcgc
     7981 tgcaaagcaa actggatggc tttctggccg cgaaagacct gatggcgcag ggcattaaaa
     8041 tcggtacctg catcacggga gatgcactag ttgccctacc cgagggcgag tcggtacgca
     8101 tcgccgacat cgtgccgggt gcgcggccca acagtgacaa cgccatcgac ctgaaagtcc
     8161 ttgaccggca tggcaatccc gtgctcgccg accggctgtt ccactccggc gagcatccgg
     8221 tgtacacggt gcgtacggtc gaaggtctgc gtgtgacggg caccgcgaac cacccgttgt
     8281 tgtgtttggt cgacgtcgcc ggggtgccga ccctgctgtg gaagctgatc gacgaaatca
     8341 agccgggcga ttacgcggtg attcaacgca gcgcattcag cgtcgactgt gcaggttttg
     8401 cccgcgggaa acccgaattt gcgcccacaa cctacacagt cggcgtccct ggactggtgc
     8461 gtttcttgga agcacaccac cgagacccgg acgcccaagc tatcgccgac gagctgaccg
     8521 acgggcggtt ctactacgcg aaagtcgcca gtgtcaccga cgccggcgtg cagccggtgt
     8581 atagccttcg tgtcgacacg gcagaccacg cgtttatcac gaacgggttc gtcagccacg
     8641 ctactggcct caccggtctg aactcaggcc tcacgacaaa tcctggtgta tccgcttggc
     8701 aggtcaacac agcttatact gcgggacaat tggtcacata taacggcaag acgtataaat
     8761 gtttgcagcc ccacacctcc ttggcaggat gggaaccatc caacgttcct gccttgtggc
     8821 agcttcaaaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg
     8881 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     8941 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     9001 ccggat
//