pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

LOCUS       pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)                9864 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G
            mutation in the ADAR deaminase domain confers increased specificity 
            with slightly reduced activity..
ACCESSION   .
VERSION     .
KEYWORDS    pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung 
            J, Zhang F
  TITLE     RNA editing with CRISPR-Cas13.
  JOURNAL   Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
  PUBMED    29070703
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     9..30
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(149..169)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        154..483
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      334..469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     396..415
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             550..1344
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(604..623)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1214..1233
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1518..1639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1555..1574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1609..1628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1688..1704)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1688..1704)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1701..1723)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1712..1728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1736..1766)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1781..1802
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1919..1936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2090..2678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2170..2189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2849..3709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3472..3491
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3710..3814)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3889..3908)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4080..4459
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4460..4663
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4614..4634
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4708..4727
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4708..4726
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8050..8076
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(9300..9317)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9306..9530
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9576..140
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9663..9682)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
       61 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      121 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      181 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      241 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      301 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      361 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      421 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      481 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
      541 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
      601 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
      661 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
      721 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
      781 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
      841 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
      901 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
      961 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1021 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1081 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1141 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1201 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1261 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1321 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     1381 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     1441 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     1501 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1561 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1621 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     1681 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1741 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1801 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     1861 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     1921 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1981 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2041 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2101 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2161 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2221 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2281 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2341 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2401 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2461 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2521 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2581 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2641 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2701 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2761 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2821 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2881 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2941 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3001 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3061 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3121 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3181 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3241 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3301 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3361 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3421 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3481 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3541 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3601 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3661 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3721 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3781 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3841 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     3901 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     3961 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     4021 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     4081 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4141 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4201 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4261 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4321 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4381 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4441 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     4501 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     4561 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     4621 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
     4681 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
     4741 ctagcgttta aacttaagct tgccaccatg aacatccccg ctctggtgga aaaccagaag
     4801 aagtactttg gcacctacag cgtgatggcc atgctgaacg ctcagaccgt gctggaccac
     4861 atccagaagg tggccgatat tgagggcgag cagaacgaga acaacgagaa tctgtggttt
     4921 caccccgtga tgagccacct gtacaacgcc aagaacggct acgacaagca gcccgagaaa
     4981 accatgttca tcatcgagcg gctgcagagc tacttcccat tcctgaagat catggccgag
     5041 aaccagagag agtacagcaa cggcaagtac aagcagaacc gcgtggaagt gaacagcaac
     5101 gacatcttcg aggtgctgaa gcgcgccttc ggcgtgctga agatgtacag ggacctgacc
     5161 aacgcataca agacctacga ggaaaagctg aacgacggct gcgagttcct gaccagcaca
     5221 gagcaacctc tgagcggcat gatcaacaac tactacacag tggccctgcg gaacatgaac
     5281 gagagatacg gctacaagac agaggacctg gccttcatcc aggacaagcg gttcaagttc
     5341 gtgaaggacg cctacggcaa gaaaaagtcc caagtgaata ccggattctt cctgagcctg
     5401 caggactaca acggcgacac acagaagaag ctgcacctga gcggagtggg aatcgccctg
     5461 ctgatctgcc tgttcctgga caagcagtac atcaacatct ttctgagcag gctgcccatc
     5521 ttctccagct acaatgccca gagcgaggaa cggcggatca tcatcagatc cttcggcatc
     5581 aacagcatca agctgcccaa ggaccggatc cacagcgaga agtccaacaa gagcgtggcc
     5641 atggatatgc tcaacgaagt gaagcggtgc cccgacgagc tgttcacaac actgtctgcc
     5701 gagaagcagt cccggttcag aatcatcagc gacgaccaca atgaagtgct gatgaagcgg
     5761 agcagcgaca gattcgtgcc tctgctgctg cagtatatcg attacggcaa gctgttcgac
     5821 cacatcaggt tccacgtgaa catgggcaag ctgagatacc tgctgaaggc cgacaagacc
     5881 tgcatcgacg gccagaccag agtcagagtg atcgagcagc ccctgaacgg cttcggcaga
     5941 ctggaagagg ccgagacaat gcggaagcaa gagaacggca ccttcggcaa cagcggcatc
     6001 cggatcagag acttcgagaa catgaagcgg gacgacgcca atcctgccaa ctatccctac
     6061 atcgtggaca cctacacaca ctacatcctg gaaaacaaca aggtcgagat gtttatcaac
     6121 gacaaagagg acagcgcccc actgctgccc gtgatcgagg atgatagata cgtggtcaag
     6181 acaatcccca gctgccggat gagcaccctg gaaattccag ccatggcctt ccacatgttt
     6241 ctgttcggca gcaagaaaac cgagaagctg atcgtggacg tgcacaaccg gtacaagaga
     6301 ctgttccagg ccatgcagaa agaagaagtg accgccgaga atatcgccag cttcggaatc
     6361 gccgagagcg acctgcctca gaagatcctg gatctgatca gcggcaatgc ccacggcaag
     6421 gatgtggacg ccttcatcag actgaccgtg gacgacatgc tgaccgacac cgagcggaga
     6481 atcaagagat tcaaggacga ccggaagtcc attcggagcg ccgacaacaa gatgggaaag
     6541 agaggcttca agcagatctc cacaggcaag ctggccgact tcctggccaa ggacatcgtg
     6601 ctgtttcagc ccagcgtgaa cgatggcgag aacaagatca ccggcctgaa ctaccggatc
     6661 atgcagagcg ccattgccgt gtacgatagc ggcgacgatt acgaggccaa gcagcagttc
     6721 aagctgatgt tcgagaaggc ccggctgatc ggcaagggca caacagagcc tcatccattt
     6781 ctgtacaagg tgttcgcccg cagcatcccc gccaatgccg tcgagttcta cgagcgctac
     6841 ctgatcgagc ggaagttcta cctgaccggc ctgtccaacg agatcaagaa aggcaacaga
     6901 gtggatgtgc ccttcatccg gcgggaccag aacaagtgga aaacacccgc catgaagacc
     6961 ctgggcagaa tctacagcga ggatctgccc gtggaactgc ccagacagat gttcgacaat
     7021 gagatcaagt cccacctgaa gtccctgcca cagatggaag gcatcgactt caacaatgcc
     7081 aacgtgacct atctgatcgc cgagtacatg aagagagtgc tggacgacga cttccagacc
     7141 ttctaccagt ggaaccgcaa ctaccggtac atggacatgc ttaagggcga gtacgacaga
     7201 aagggctccc tgcagcactg cttcaccagc gtggaagaga gagaaggcct ctggaaagag
     7261 cgggcctcca gaacagagcg gtacagaaag caggccagca acaagatccg cagcaaccgg
     7321 cagatgagaa acgccagcag cgaagagatc gagacaatcc tggataagcg gctgagcaac
     7381 agccggaacg agtaccagaa aagcgagaaa gtgatccggc gctacagagt gcaggatgcc
     7441 ctgctgtttc tgctggccaa aaagaccctg accgaactgg ccgatttcga cggcgagagg
     7501 ttcaaactga aagaaatcat gcccgacgcc gagaagggaa tcctgagcga gatcatgccc
     7561 atgagcttca ccttcgagaa aggcggcaag aagtacacca tcaccagcga gggcatgaag
     7621 ctgaagaact acggcgactt ctttgtgctg gctagcgaca agaggatcgg caacctgctg
     7681 gaactcgtgg gcagcgacat cgtgtccaaa gaggatatca tggaagagtt caacaaatac
     7741 gaccagtgca ggcccgagat cagctccatc gtgttcaacc tggaaaagtg ggccttcgac
     7801 acataccccg agctgtctgc cagagtggac cgggaagaga aggtggactt caagagcatc
     7861 ctgaaaatcc tgctgaacaa caagaacatc aacaaagagc agagcgacat cctgcggaag
     7921 atccggaacg ccttcgatgc aaacaattac cccgacaaag gcgtggtgga aatcaaggcc
     7981 ctgcctgaga tcgccatgag catcaagaag gcctttgggg agtacgccat catgaaggga
     8041 agcctgcagc tgcctccact tgaaagactg acactgggat ccggaggagg tggaagccag
     8101 ctgcatttac cgcaggtttt agctgacgct gtctcacgcc tggtcctggg taagtttggt
     8161 gacctgaccg acaacttctc ctcccctcac gctcgcagaa aagtgctggc tggagtcgtc
     8221 atgacaacag gcacagatgt taaagatgcc aaggtgataa gtgtttctac aggaggcaaa
     8281 tgtattaatg gtgaatacat gagtgatcgt ggccttgcat taaatgactg ccatgcagaa
     8341 ataatatctc ggagatcctt gctcagattt ctttatacac aacttgagct ttacttaaat
     8401 aacaaagatg atcaaaaaag atccatcttt cagaaatcag agcgaggggg gtttaggctg
     8461 aaggagaatg tccagtttca tctgtacatc agcacctctc cctgtggaga tgccagaatc
     8521 ttctcaccac atgagccaat cctggaagaa ccagcagata gacacccaaa tcgtaaagca
     8581 agaggacagc tacggaccaa aatagagtct ggtcagggga cgattccagt gcgctccaat
     8641 gcgagcatcc aaacgtggga cggggtgctg caaggggagc ggctgctcac catgtcctgc
     8701 agtgacaaga ttgcacgctg gaacgtggtg ggcatccagg gatcactgct cagcattttc
     8761 gtggagccca tttacttctc gagcatcatc ctgggcagcc tttaccacgg ggaccacctt
     8821 tccagggcca tgtaccagcg gatctccaac atagaggacc tgccacctct ctacaccctc
     8881 aacaagcctt tgctcagtgg catcagcaat gcagaagcac ggcagccagg gaaggccccc
     8941 aacttcagtg tcaactggac ggtaggcgac tccgctattg aggtcatcaa cgccacgact
     9001 gggaaggatg agctgggccg cgcgtcccgc ctgtgtaagc acgcgttgta ctgtcgctgg
     9061 atgcgtgtgc acggcaaggt tccctcccac ttactacgct ccaagattac caagcccaac
     9121 gtgtaccatg agtccaagct ggcggcaaag gagtaccagg ccgccaaggc gcgtctgttc
     9181 acagccttca tcaaggcggg gctgggggcc tgggtggaga agtccaccga gcaggaccag
     9241 ttctcactca cgtaagcggc cgctcgagcc tagagggccc gtttaaaccc gctgatcagc
     9301 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     9361 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     9421 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     9481 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     9541 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     9601 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     9661 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     9721 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9781 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9841 ccctttgacg ttggagtcca cgtt
//