| 质粒名称: | pLU-CMV-NeonOxIrr_C199S |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000163 |
| 产品用途: | Expresses the mutant of the NeonOxIrr/C199S irreversible green indicator for hydrogen peroxide with blocked sensitivity to hydrogen peroxide in mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pLU |
| 表达系统: | 慢病毒，哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
|
插入基因信息 |
|
| 插入基因名称: |
NeonOxIrr/C199S
|
| 物种来源: |
Synthetic
|
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Slowly Reducible Genetically Encoded Green Fluorescent Indicator for In Vivo and Ex Vivo Visualization of Hydrogen Peroxide
Subach O.M., Kunitsyna T.A., Mineyeva O., Lazutkin A.A., Bezryadnov D., Barykina N.V., Piatkevich K.D., Ermakova Y., Bilan D., Belousov V., Anokhin K.V., Enikolopov G.N., Subach F.V.
Int. J. Mol. Sci. 2019, Vol. 20, Page 3138 20, 3138 (2019)
|
买家导航
pLU-CMV-NeonOxIrr_C199S载体质粒基本信息
pLU-CMV-NeonOxIrr_C199S质粒图谱载体图谱和pLU-CMV-NeonOxIrr_C199S载体序列质粒序列多克隆位点信息
pLU-CMV-NeonOxIrr_C199S质粒载体简介
pLU-CMV-NeonOxIrr_C199S质粒序列载体序列
LOCUS pLU-CMV-NeonOxIrr_C199S 7935 bp DNA circular SYN 22-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7935)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7935
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 234..414
/label=5' LTR
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 458..583
/label=HIV-1 psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1076..1309
/label=HIV-1 RRE
/note="The Rev response element (RRE) of human
immunodeficiency virus type 1 allows for Rev-dependent mRNA
export from the nucleus to the cytoplasm."
misc_feature 1798..1915
/label=HIV-1 cPPT
/note="central polypurine tract from pol gene of human
immunodeficiency virus type 1"
enhancer 1936..2241
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2242..2445
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2542..3975
/codon_start=1
/label=NeonOxIrr/C199S
/translation="MEMASQQGETMSGPLHIGLISTVGPYLLPHIIPMLHQTFPKLEMY
LHEAQTHQLLAQLDSGKLDCVILAPVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVP
MADLAGEKLLMLEDGHSLRDQAMAYFADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYR
STARTTYTFAKPMAANYLKNQPMYVVRKTELKHSKTELNFKEWQKAFTDVMGMDELYKV
DGGSGGTGMASKGEEDNMASLPATHELHIFGSINGADFDMVGQGTGNPIDGYEELNLKS
TKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTV
NYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAIDNGFCFEAGADEDTHFRATSLET
LRNMVAAGSGITLLPALAVPPERKRDGVVYLPCTKPEPRRTIGLVYRPGSPLRSRYEQL
AEAIRARMDGHFDKVLKQAV"
misc_feature 4022..4610
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(4493..4504)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 4697..4930
/label=3'LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
LTR 4750..4930
/label=5' LTR
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 5002..5123
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 5142..5277
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(5310..5326)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 5334..5350
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5358..5388)
/label=lac promoter
/note="promoter for the E. coli lac operon"
rep_origin complement(5712..6300)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6471..7331)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7332..7436)
/gene="bla"
/label=AmpR promoter
primer_bind 7910..7926
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
901 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
1921 taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
2461 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgacggcgc
2521 gccaccatgg ctagcctcga gatggagatg gcaagccagc agggcgagac gatgtccgga
2581 ccgctgcaca ttggtttgat ttccacagtt ggaccgtacc tgctaccgca tattatccct
2641 atgctgcacc agacctttcc aaagctggaa atgtatctgc atgaagcgca gacccaccag
2701 ttactggcgc aactggacag cggcaaactc gattgcgtga tcctcgcgcc ggtgaaagag
2761 agcgaagcat tcattgaagt gccgttgttt gatgagccaa tgttgctggc tatctatgaa
2821 gatcacccgt gggcgaaccg cgagtgcgta ccgatggccg atctggcagg ggaaaaactg
2881 ctgatgctgg aagatggcca ctctttgcgc gatcaggcaa tggcgtactt cgctgactgg
2941 tgtagatcca agaagactta ccccaatgat aagactatca tttccacatt taagtggtct
3001 tataccaccg gcaacggcaa acgctaccgg tctaccgctc gcaccacata cacattcgcc
3061 aaaccaatgg ccgcaaatta cctcaaaaat cagccgatgt atgtcgttcg gaaaacagag
3121 ctgaaacatt ctaagaccga actgaatttt aaagagtggc aaaaggcctt tacggacgtg
3181 atgggcatgg acgagctgta taaggtggat ggcggtagcg gtggcaccgg catggcctca
3241 aagggagagg aggacaacat ggctagtctg cctgctaccc acgaactgca tatttttggc
3301 tccataaatg gcgccgattt cgacatggta ggccaaggca ccggtaatcc aatcgacggt
3361 tatgaggagc tgaacctgaa gtccaccaag ggcgatctgc agttcagtcc ctggatcctg
3421 gtgccgcaca ttgggtacgg gtttcatcag tatcttccct atccagacgg gatgtcacca
3481 tttcaggctg caatggtcga cgggtccgga tatcaggtgc accgcactat gcagtttgag
3541 gacggtgctt cactgaccgt gaattacaga tacacctacg aaggatcaca tatcaaaggg
3601 gaagctcagg tgaagggcac tgggttccca gctgacggcc ctgtgatgac aaattccctg
3661 acagccatcg acaacggttt ctgttttgaa gccggggcgg atgaagatac acacttccgc
3721 gcgactagcc tggaaactct gcgcaacatg gtagcggcag gtagcgggat cactttactg
3781 ccagcgctgg ctgtgccgcc ggagcgcaaa cgcgatgggg ttgtttatct gccgtgcact
3841 aagccggaac cacgccgcac tattggcctg gtttatcgtc ctggctcacc gctgcgcagc
3901 cgctatgagc agctggctga ggccatccgc gcaagaatgg atggccattt cgataaagtt
3961 ttaaaacagg cggtttaaac cggtatccgt ctagatgtac aagtaaagcg gccgcgtcga
4021 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
4081 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
4141 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
4201 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
4261 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
4321 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
4381 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
4441 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
4501 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
4561 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg
4621 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
4681 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
4741 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
4801 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
4861 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
4921 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
4981 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
5041 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
5101 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
5161 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
5221 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
5281 gcttttgcgg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
5341 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
5401 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
5461 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
5521 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
5581 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
5641 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
5701 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
5761 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
5821 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
5881 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
5941 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
6001 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
6061 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
6121 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
6181 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
6241 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
6301 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
6361 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
6421 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
6481 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
6541 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
6601 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
6661 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
6721 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
6781 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
6841 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
6901 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
6961 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
7021 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
7081 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
7141 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
7201 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
7261 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
7321 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
7381 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
7441 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
7501 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
7561 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
7621 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
7681 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
7741 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
7801 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
7861 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
7921 gccagtgcca agctg
//
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