pYK213

LOCUS       pYK213                8436 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     881..987
                     /label=oYK213_G3BP1_F
     primer_bind     881..904
                     /label=oYK432_NHE_F
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     primer_bind     881..900
                     /label=oYK30-NS3a_Head-F
     primer_bind     881..900
                     /label=oYK165_G3BP1_cut_F
     promoter        881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             905..964
                     /codon_start=1
                     /label=NLS
                     /translation="MPKKKRKVEASASPKKKRKV"
     primer_bind     964..987
                     /label=oQZ238
     primer_bind     964..987
                     /label=oCW18
     primer_bind     964..987
                     /label=oCW20
     gene            965..2362
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /label=G3BP1
                     /note="G3BP stress granule assembly factor 1"
                     /db_xref="GeneID:10146"
                     /db_xref="HGNC:HGNC:30292"
                     /db_xref="MIM:608431"
     misc_feature    965..1390
                     /label=NTF2L
     misc_feature    1391..1642
                     /label=IDR1
     primer_bind     1391..1410
                     /label=oYK165_G3BP1_cut_F
     misc_feature    1391..1393
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Phosphothreonine./evidence=ECO:0007744|PubMed:186696
                     48; propagated from UniProtKB/Swiss-Prot (Q13283.1); 
                     phosphorylation site"
     misc_feature    1409..1411
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Phosphoserine./evidence=ECO:0000269|PubMed:11604510,
                      ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:17693683, 
                     ECO:0007744|PubMed:18220336, ECO:0007744|PubMed:18318008, 
                     ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976, 
                     ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, 
                     ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569; 
                     propagated from UniProtKB/Swiss-Prot (Q13283.1); 
                     phosphorylation site"
     misc_feature    1643..1966
                     /label=IDR2
     misc_feature    1655..1657
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Phosphoserine. 
                     /evidence=ECO:0000269|Ref.6;propagated from 
                     UniProtKB/Swiss-Prot (Q13283.1); phosphorylation site"
     misc_feature    1658..1660
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Phosphoserine./evidence=ECO:0000269|PubMed:11604510,
                      ECO:0000269|Ref.6, ECO:0007744|PubMed:18669648, 
                     ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, 
                     ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:24275569; 
                     propagated from UniProtKB/Swiss-Prot (Q13283.1); 
                     phosphorylation site"
     misc_feature    1712..1714
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Phosphoserine./evidence=ECO:0007744|PubMed:23186163;
                      propagated from UniProtKB/Swiss-Prot (Q13283.1); 
                     phosphorylation site"
     misc_feature    1721..1723
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Phosphoserine./evidence=ECO:0007744|PubMed:23186163;
                      propagated from UniProtKB/Swiss-Prot (Q13283.1); 
                     phosphorylation site"
     primer_bind     1930..1940
                     /label=Oscl9-1
                     /note="for primer of EGFP"
     misc_feature    1937..1942
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Cleavage, by human enterovirus 71 protease 3C. 
                     /evidence=ECO:0000269|PubMed:30006004; propagated from 
                     UniProtKB/Swiss-Prot (Q13283.1); cleavage site"
     misc_feature    1967..2194
                     /label=RRM
     misc_feature    2081..2083
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Phosphoserine./evidence=ECO:0007744|PubMed:18669648,
                      ECO:0007744|PubMed:20068231; propagated from 
                     UniProtKB/Swiss-Prot (Q13283.1); phosphorylation site"
     misc_feature    2090..2092
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:19608861; propagated from 
                     UniProtKB/Swiss-Prot (Q13283.1); acetylation site"
     misc_feature    2195..2362
                     /label=IDR3
     misc_feature    2249..2251
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Asymmetric dimethylarginine. 
                     /evidence=ECO:0007744|PubMed:24129315; propagated from 
                     UniProtKB/Swiss-Prot (Q13283.1); methylation site"
     misc_feature    2267..2269
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Asymmetric dimethylarginine, alternate. 
                     /evidence=ECO:0007744|PubMed:24129315; Dimethylated 
                     arginine, alternate. /evidence=ECO:0000269|Ref.6; 
                     Omega-N-methylarginine, alternate. 
                     /evidence=ECO:0000269|Ref.6, ECO:0007744|PubMed:24129315; 
                     propagated from UniProtKB/Swiss-Prot (Q13283.1); 
                     methylation site"
     misc_feature    2303..2305
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Omega-N-methylarginine. 
                     /evidence=ECO:0007744|PubMed:24129315; propagated from 
                     UniProtKB/Swiss-Prot (Q13283.1); methylation site"
     primer_bind     complement(2318..2364)
                     /label=oCW21
     misc_feature    2342..2344
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Dimethylated arginine, alternate. 
                     /evidence=ECO:0000269|Ref.6; Omega-N-methylarginine, 
                     alternate. /evidence=ECO:0000269|Ref.6, 
                     ECO:0007744|PubMed:24129315; propagated from 
                     UniProtKB/Swiss-Prot (Q13283.1); methylation site"
     primer_bind     complement(2352..2382)
                     /label=oYK79_G3BP1_R
     misc_feature    2357..2359
                     /gene="G3BP1"
                     /gene_synonym="G3BP; HDH-VIII"
                     /note="Omega-N-methylarginine. 
                     /evidence=ECO:0007744|PubMed:24129315; propagated from 
                     UniProtKB/Swiss-Prot (Q13283.1); methylation site"
     misc_feature    2363..2590
                     /label=Long_Linker
     primer_bind     2363..2382
                     /label=oYK78_AG_ANR1_F
     primer_bind     2363..2380
                     /label=oYK69_AG_ANR1_F
     misc_feature    2363..2364
                     /label=linker
     misc_feature    2591..3265
                     /label=AG
     primer_bind     3039..3049
                     /label=oSCL1104
     primer_bind     complement(3253..3273)
                     /label=oYK65-AG-R
     misc_feature    3266..3316
                     /label=Short_Linker
     primer_bind     complement(3292..3316)
                     /label=oYK33-35-PB1-AG-R
     primer_bind     3297..3337
                     /label=oYK153_NS3a_F
     CDS             3317..3904
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     3317..3352
                     /label=oSCL1082
     primer_bind     3317..3337
                     /label=oyk03-03-F
     primer_bind     3317..3337
                     /label=oYK04-ns3a-F
     primer_bind     3317..3335
                     /label=oYK25-NS3a-F
     primer_bind     3317..3334
                     /label=oYK30-NS3a_Head-F
     primer_bind     3334..3392
                     /label=oSCL1083
     primer_bind     3373..3431
                     /label=oSCL1084
     primer_bind     3410..3468
                     /label=oSCL1085
     primer_bind     3447..3505
                     /label=oSCL1086
     primer_bind     complement(3475..3494)
                     /label=oSCL1106
     primer_bind     3476..3514
                     /label=oSCL1104
     primer_bind     complement(3711..3769)
                     /label=oSCL1105
     primer_bind     complement(3751..3822)
                     /label=oSCL1087
     primer_bind     3753..3774
                     /label=oSCL1107
     primer_bind     3804..3822
                     /label=oSCL1088
     primer_bind     complement(3885..3910)
                     /label=oYK433_NS3a2_R
     primer_bind     complement(3886..3933)
                     /label=oSCL1089
     primer_bind     complement(3889..3936)
                     /label=oYK30-NS3a_head-R
     primer_bind     complement(3905..3953)
                     /label=oYK03-03-R
     primer_bind     complement(3905..3924)
                     /label=oSCL1130
     primer_bind     complement(3913..3933)
                     /label=olyl382
     primer_bind     complement(3934..3956)
                     /label=oYK57-MCP-mCherry-R
     primer_bind     complement(3934..3953)
                     /label=oYK25-NS3a-R
     primer_bind     complement(3958..3977)
                     /label=oSCL1089
     terminator      4030..4257
                     /label=bGH PA
     misc_feature    4303..4731
                     /label=f1 origin
     misc_feature    4736..5079
                     /label=SV40 early promoter and origin
     misc_feature    4925..5002
                     /label=SV40 ORI
     CDS             5144..5935
                     /label=Kan/neoR
     misc_feature    6109..6239
                     /label=SV40 early polyadenylation signal
     primer_bind     6213..6232
                     /label=olyl382
     primer_bind     complement(6275..6295)
                     /label=M13-rev
     misc_binding    complement(6301..6323)
                     /label=LacO
     promoter        complement(6328..6357)
                     /label=lac
     primer_bind     6472..6483
                     /label=oYK69_AG_ANR1_F
     misc_feature    complement(6622..7295)
                     /label=pUC origin
     CDS             complement(7443..8102)
                     /label=AmpR
     primer_bind     7759..7770
                     /label=oyk03-03-F
     primer_bind     7759..7770
                     /label=oYK04-ns3a-F
     primer_bind     7759..7770
                     /label=oYK153_NS3a_F
     promoter        complement(8342..8370)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
      961 agtcatggtg atggagaagc ctagtcccct gctggtcggg cgggaatttg tgagacagta
     1021 ttacacactg ctgaaccagg ccccagacat gctgcataga ttttatggaa agaactcttc
     1081 ttatgtccat gggggattgg attcaaatgg aaagccagca gatgcagtct acggacagaa
     1141 agaaatccac aggaaagtga tgtcacaaaa cttcaccaac tgccacacca agattcgcca
     1201 tgttgatgct catgccacgc taaatgatgg tgtggtagtc caggtgatgg ggcttctctc
     1261 taacaacaac caggctttga ggagattcat gcaaacgttt gtccttgctc ctgaggggtc
     1321 tgttgcaaat aaattctatg ttcacaatga tatcttcaga taccaagatg aggtctttgg
     1381 tgggtttgtc actgagcctc aggaggagtc tgaagaagaa gtagaggaac ctgaagaaag
     1441 acagcaaaca cctgaggtgg tacctgatga ttctggaact ttctatgatc aggcagttgt
     1501 cagtaatgac atggaagaac atttagagga gcctgttgct gaaccagagc ctgatcctga
     1561 accagaacca gaacaagaac ctgtatctga aatccaagag gaaaagcctg agccagtatt
     1621 agaagaaact gcccctgagg atgctcagaa gagttcttct ccagcacctg cagacatagc
     1681 tcagacagta caggaagact tgaggacatt ttcttgggca tctgtgacca gtaagaatct
     1741 tccacccagt ggagctgttc cagttactgg gataccacct catgttgtta aagtaccagc
     1801 ttcacagccc cgtccagagt ctaagcctga atctcagatt ccaccacaaa gacctcagcg
     1861 ggatcaaaga gtgcgagaac aacgaataaa tattcctccc caaaggggac ccagaccaat
     1921 ccgtgaggct ggtgagcaag gtgacattga accccgaaga atggtgagac accctgacag
     1981 tcaccaactc ttcattggca acctgcctca tgaagtggac aaatcagagc ttaaagattt
     2041 ctttcaaagt tatggaaacg tggtggagtt gcgcattaac agtggtggga aattacccaa
     2101 ttttggtttt gttgtgtttg atgattctga gcctgttcag aaagtcctta gcaacaggcc
     2161 catcatgttc agaggtgagg tccgtctgaa tgtcgaagag aagaagactc gagctgccag
     2221 ggaaggcgac cgacgagata atcgccttcg gggacctgga ggccctcgag gtgggctggg
     2281 tggtggaatg agaggccctc cccgtggagg catggtgcag aaaccaggat ttggagtggg
     2341 aagggggctt gcgccacggc agggttctgg gagtggcggt agtggtgctg gaggcagcgc
     2401 aggctctgga gcaggcggca gtgccggaag tggcgctgga gggtctgccg gatctggagc
     2461 cggtggttct gccggctccg gcgctggtgg gagcgcttcc ggaagtagtc ccggtaattc
     2521 cgctgacggc ggcggaggat cgggtggtag tggtggttca ggaggaggat cgacccaagg
     2581 aggatccatg gtgagcgtga tcaagcccga gatgaagatc aagctgtgca tgaggggcac
     2641 cgtgaacggc cacaacttcg tgatcgaggg cgagggcaag ggcaacccct acgagggcac
     2701 ccagatcctg gacctgaacg tgaccgaggg cgcccccctg cccttcgcct acgacatcct
     2761 gaccaccgtg ttccagtacg gcaacagggc cttcaccaag taccccgccg acatccagga
     2821 ctacttcaag cagaccttcc ccgagggcta ccactgggag aggagcatga cctacgagga
     2881 ccagggcatc tgcaccgcca ccagcaacat cagcatgagg ggcgactgct tcttctacga
     2941 catcaggttc gacggcgtga acttcccccc caacggcccc gtgatgcaga agaagaccct
     3001 gaagtgggag cccagcaccg agaagatgta cgtgagggac ggcgtgctga agggcgacgt
     3061 gaacatggcc ctgctgctgg agggcggcgg ccactacagg tgcgacttca agaccaccta
     3121 caaggccaag aaggacgtga ggctgcccga ctaccacttc gtggaccaca ggatcgagat
     3181 cctgaagcac gacaaggact acaacaaggt gaagctgtac gagaacgccg tggccaggta
     3241 cagcatgctg cccagccagg ccaagggtac cggaactgca gcagagaatt cgggaaactc
     3301 gagaacaaag cttgaaaaga agaagggcag cgtggtgatc gtgggcagaa tcaacctgag
     3361 cggcgacacc gcctacagcc agcagaccag aggcctggag ggctgccagg agaccagcca
     3421 gaccggcaga gacaagaacc aggtggaggg cgaggtgcag gtggtgagca ccgccaccca
     3481 gagcttcctg gccaccagca tcaacggcgt gctgtggacc gtgtaccacg gcgccggcac
     3541 cagaaccatc gccagcccca agggccccgt gacccagatg tacacgaacg tggacaagga
     3601 cttagtgggc tggcaggccc cccaaggcag cagaagcctg accccctgca cctgcggcag
     3661 cagcgacctg tacctggtga ccagacacgc cgacgtgatc cccgtgagag gaagaggcga
     3721 cagcagaggc agcctgctga gccccagacc catcagctat ctgaaaggct caagcggcgg
     3781 ccccctgttg tgtcccgccg gccatgccgt gggcatcttc agagccgccg tgagcaccag
     3841 aggcgtggcc aaggccgtgg acttcatccc cgtggagagc ctggagacca ccatgagaag
     3901 ccccgctagc tagaaattct gcagatatcc agcggatcca ctagtccagt gtggtggaat
     3961 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct
     4021 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4081 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     4141 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     4201 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     4261 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     4321 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4381 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4441 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4501 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4561 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4621 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4681 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     4741 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4801 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4861 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4921 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4981 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     5041 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     5101 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     5161 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     5221 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     5281 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     5341 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     5401 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5461 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5521 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5581 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5641 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     5701 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5761 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5821 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5881 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     5941 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     6001 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6061 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     6121 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6181 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     6241 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6301 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6361 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6421 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6481 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6541 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6601 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6661 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6721 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6781 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6841 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6901 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6961 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7021 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7081 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7141 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7201 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7261 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7321 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7381 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7441 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7501 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7561 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7621 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7681 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7741 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7801 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7861 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7921 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7981 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8041 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8101 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8161 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8221 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8281 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8341 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8401 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//