| 质粒名称: | pGEX-6p-1-GTBP1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000921 |
| 产品用途: | Express proteins in vitro |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX-6p-1 |
| 表达系统: | 细菌表达 |
| 启动子: | tac, AmpR, Lac, LacIq |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, RosettaDE3, BL21 |
|
插入基因信息 |
|
| 插入基因名称: | GTBP1 |
| 物种来源: | C.elegans |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network
Yao Z., Liu Y., Chen Q., Chen X., Zhu Z., Song S., Ma X., Yang P.
Cell Rep. 43, 114617 (2024)
|
买家导航
pGEX-6p-1-GTBP1载体质粒基本信息
pGEX-6p-1-GTBP1质粒图谱载体图谱和pGEX-6p-1-GTBP1载体序列质粒序列多克隆位点信息
pGEX-6p-1-GTBP1质粒载体简介
pGEX-6p-1-GTBP1质粒序列载体序列
LOCUS pGEX-6p-1-GTBP1 6415 bp DNA circular SYN 07-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6415)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6415
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..941
/codon_start=1
/product="recognition and cleavage site for human
rhinovirus 3C and PreScission proteases"
/label=HRV 3C site
/translation="LEVLFQGP"
CDS 951..971
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
misc_feature 978..2396
/label=codon GTBP1
promoter 2718..2822
/gene="bla"
/label=AmpR promoter
CDS 2823..3683
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3854..4442
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 4686..4763
/gene="lacI"
/label=lacI promoter
CDS 4764..5846
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter 5895..5925
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5933..5949
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5957..5973
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS join(5969..6415,1..81)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
QPSEAVVWLCRS"
primer_bind complement(5989..6005)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc gaaaacctgt
961 atttccaggg cggcagcatg cagattacca gtgccctgac ccagaataag atggacccta
1021 ttaatggtaa tctgaccagc accgcaccgg ttgaaccgct gagcctgacc gccgcagaac
1081 aggttggcgg tgcattttgc catcagtttt atattaccgt tagtgaaaac cgtaaggcaa
1141 ttaccaaatt ttatggccat gaaagtaagt tttacctgga tgatcaggtg gtgaccggca
1201 gccaggaaat tgtgaaactg tataatcatc tgccggaaac cacccatttt aaaattcaga
1261 gtattaaggg ttacccgacc ccgcataaac agggtgttat tattaatgtt atcggtacag
1321 ttaacctgcg cccgtttctg cagagctttc tgctgggtca gcagggtcag aaaaaatatt
1381 atgtggaaag cgatgccttt cagtatattg ataaattttt cgtggcgaat accgaaaaag
1441 aaaaaaccac cagcagtacc ggccataatg gtcatgcaag tggcgcaaat ggcaaagata
1501 gcaaaagtag taaaaccgaa cagccgaaag aacagagcaa accggccccg ccgaaacagc
1561 agaccccgaa aaaagcagaa gatcgtattg tgaaacagga taccccgaaa agcgttgaaa
1621 aagaagttgc caaaaccccg cagaaaccgc agctggcaca gcagccgccg cagcaacgtg
1681 cccctgtgcc tgaaccggtt caggaacagc cgaaggttca gaaaacctgg gccaatctgg
1741 ttggtggtgg cggtaaaagc cagccgccgc aacagcagag ctatggtcag ggccagcaga
1801 ttcatcagca gacccagatg catcacatgc agccgagtat gcatcagcag cagcagggta
1861 gccatgcacc gctgtatcag cagcagcgtc agagtcatcc ggcaaatgaa ctgcgccagg
1921 cccaggaaag tagtgatgcc aatctgaatg aacgcaaaat ctatctgggc ggtattaccc
1981 gtgaaattgt tccgagtaat accgcacagg cagaaagcga aattaagcag gttttctata
2041 aatacggtga aattgaagag gttcgtatgc cgcgcaaagt gctggatagc ccgaatgata
2101 ccagcaaaca tgcctttgcc tttattacca tgcgtaataa ggccggtgtt aaagccattt
2161 ttgaagccgc caccaaagat gaacagggtg tttggaaact gccgctgaaa ctggatgcat
2221 ttggctttga tggccatgca cagattagcg aacagcgcga tcagccgcag ggcggtggct
2281 ttcgtggcgg tcgtggcggt tttcagccgc gtggtggtca gtttttccgc cgcggcggca
2341 gcggtggcgc tcctcgtggt ggtatgcgca gtggttttca gaatgcaggt cagaattaac
2401 tcgagcggcc gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg
2461 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
2521 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
2581 tgacccagtc acgtagcgat agcggagtgt ataattcttg aagacgaaag ggcctcgtga
2641 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
2701 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
2761 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
2821 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
2881 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
2941 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
3001 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
3061 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
3121 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
3181 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
3241 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
3301 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
3361 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
3421 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
3481 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
3541 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
3601 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
3661 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
3721 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
3781 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
3841 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
3901 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
3961 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
4021 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
4081 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
4141 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
4201 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
4261 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
4321 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
4381 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
4441 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
4501 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
4561 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
4621 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcataa
4681 attccgacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag
4741 agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg
4801 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
4861 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg
4921 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc
4981 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca
5041 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca
5101 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg
5161 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc
5221 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc
5281 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct
5341 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga
5401 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc
5461 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg
5521 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat
5581 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt
5641 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
5701 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacg ctagcgccca
5761 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
5821 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat
5881 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
5941 ggataacaat ttcacacagg aaacagctat gaccatgatt acggattcac tggccgtcgt
6001 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
6061 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
6121 gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc
6181 ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg
6241 gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa
6301 tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga
6361 tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgttg gaatt
//
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