| 质粒名称: | pSL1969 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002461 |
| 产品用途: | Mammalian PhCMV-driven TetR-LOV2pep (TULIP) expression vector (PhCMV-TetR-LOV2pep-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | TetR-LOV2pep |
| 物种来源: | Synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1969
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1969载体质粒基本信息
pSL1969质粒图谱载体图谱和pSL1969载体序列质粒序列多克隆位点信息
pSL1969质粒载体简介
pSL1969质粒序列载体序列
LOCUS pSL1969 6486 bp DNA circular SYN 09-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6486)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 31, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6486
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
misc_feature 912..1529
/label=tetr
CDS 1530..1559
/codon_start=1
/label=linker
/translation="ASGSGGGGDV"
CDS 1971..1997
/codon_start=1
/label=pep
/translation="IDKAVDTWV"
terminator 2083..2310
/label=bGH PA
rep_origin 2356..2784
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2798..3127
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3194..3988
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4162..4283
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4328..4348)
/label=M13-rev
misc_binding complement(4354..4376)
/label=LacO
promoter complement(4380..4410)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4425..4446
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4734..5319)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5490..6350)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6351..6455)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagg
901 aattcgccac catgtctaga ctggacaaga gcaaagtcat aaactctgct ctggaattac
961 tcaatgaagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
1021 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctggcaa
1081 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
1141 actttctgcg gaacaacgcc aagtcattcc gctgtgctct cctctcacat cgcgacgggg
1201 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
1261 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
1321 tgggccactt tacactgggc tgcgtattgg aggatcagga gcatcaagta gcaaaagagg
1381 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
1441 tcgaccatca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
1501 tggagaaaca gctaaagtgc gaaagcggcg ccagcggaag tggcggagga ggcgacgtcg
1561 gatccttggc tgctgcactt gaacgtattg agaagaactt tgtcattact gacccaaggt
1621 tgccagataa tcccattata ttcgcgtccg atagtttctt gcagttgaca gaatatagcc
1681 gtgaagaaat tttgggaaga aactgcaggt ttctacaagg tcctgaaact gatcgcgcga
1741 cagtgagaaa aattagagat gccatagata accaaacaga ggtcactgtt cagctgatta
1801 attatacaaa gagtggtaaa aagttctgga acctctttca cttgcagcct atgcgagatc
1861 agaagggaga tgtccagtac tttattgggg ttcagttgga tggaactgag catgtccgag
1921 atgctgccga gagagaggca gtcatgctgg cgaagaaaac tgcagaagag attgataaag
1981 cggtggatac ctgggtgtaa gctagctaga aattctgcag atatccagca cagtggcggc
2041 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
2101 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
2161 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
2221 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
2281 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
2341 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
2401 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
2461 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
2521 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
2581 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
2641 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
2701 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
2761 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
2821 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
2881 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
2941 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
3001 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
3061 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
3121 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
3181 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
3241 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
3301 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
3361 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
3421 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
3481 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
3541 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
3601 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
3661 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
3721 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
3781 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
3841 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
3901 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
3961 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
4021 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
4081 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
4141 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
4201 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
4261 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
4321 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
4381 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
4441 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
4501 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
4561 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
4621 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
4681 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
4741 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
4801 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
4861 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
4921 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
4981 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
5041 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
5101 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
5161 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
5221 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt
5281 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
5341 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
5401 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
5461 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
5521 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
5581 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
5641 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
5701 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
5761 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
5821 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
5881 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
5941 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
6001 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
6061 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
6121 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
6181 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
6241 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
6301 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
6361 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
6421 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
6481 gacgtc
//
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