| 质粒名称: | CTBPro |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001047 |
| 产品用途: | To express the CTB-mBaojin fusion tracer in targeted cell populations, with in vivo expression specifically validated in endothelial cells (ECs) via AAV-Bi30 mediated delivery. |
| 存储实验室: | Laboratory of Neurovascular Biology |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 细菌表达、腺病毒表达、哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | signal peptide/CTB/mbaojin |
| 物种来源: | mus musculus |
| 密码子优化: | 哺乳细胞表达优化 |
CTBPro
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
CTBPro载体质粒基本信息
CTBPro质粒图谱载体图谱和CTBPro载体序列质粒序列多克隆位点信息
CTBPro质粒载体简介
CTBPro质粒序列载体序列
LOCUS CTBPro 5812 bp DNA circular SYN 09-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5812)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Aug 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5812
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 1367..2068
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
regulatory 980..988
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 986..1039
/product="From P07724 1-18"
/label=Signal
CDS 1040..1348
/product="P01556"
/label=CHTB_VIBCH
CDS 1349..1366
/codon_start=1
/label=Linker
/translation="GGSGGT"
CDS 1367..2068
/codon_start=1
/label=mBaoJin
/translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
EAHLKGMDELYK"
misc_feature 2096..2684
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2567..2578)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2727..2848
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3376..3964)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4135..4995)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4996..5100)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5126..5581)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtacctaag ccaccatgaa atgggtgact tttctgctct tgctgtttgt
1021 gtcaggcagc gctttctcta ctcctcaaaa catcaccgac ctgtgtgctg agtaccataa
1081 tacacagata tacacactga acgacaagat ttttagctat actgaatctc ttgcaggcaa
1141 gagggaaatg gccattatca ccttcaagaa tggggctatt ttccaggttg aggtgcctgg
1201 gagccaacac atagactctc agaagaaagc tattgagagg atgaaggaca ccctcagaat
1261 cgcctatctt actgaggcta aggtggagaa actgtgtgtg tggaataaca aaacgccaca
1321 cgcaatcgcc gcaatatcta tggcaaacgg tgggagcgga ggtaccatgg tctcaaaggg
1381 agaggaggaa aacatggcta gtacaccatt taaatttcaa cttaaaggaa ccatcaatgg
1441 caaatcgttt accgttgaag gcgaaggtga agggaactca catgaaggtt ctcataaagg
1501 aaaatatgtt tgtacaagtg gaaaactacc gatgtcatgg gcagcacttg ggacaacctt
1561 tggttatgga atgaaatatt ataccaaata tcctagtgga ctgaagaact ggtttcgtga
1621 agtaatgccc ggaggcttta cctacgatcg tcatattcaa tataaaggcg atgggagtat
1681 ccatgcaaaa caccaacact ttatgaaaaa tgggacttat cacaacattg tagaatttac
1741 cggtcaggat tttaaagaaa atagtccagt cttaactgga gatatgaatg tctcattacc
1801 gaatgaagtc ccacaaatac ccagagatga tggagtagaa tgcccagtga ccttgcttta
1861 tcctttatta tcggataaat caaaatacgt cgaggctcac caatatacaa tctgcaagcc
1921 tcttcataat caaccagcac ctgatgtccc atatcactgg attcgtaaac aatacacaca
1981 aagcaaagat gatgccgagg aacgcgatca tatctgtcaa tcagagactc tcgaagcaca
2041 cttaaagggc atggacgagc tgtataagta agaattcgat atcaagctta tcgataatca
2101 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
2161 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
2221 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
2281 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
2341 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
2401 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
2461 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
2521 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
2581 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
2641 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
2701 cgggcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
2761 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
2821 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
2881 ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
2941 aatcgataag gatcttccta gagcatggct acgtagataa gtagcatggc gggttaatca
3001 ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
3061 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
3121 tgagcgagcg agcgcgcagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
3181 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
3241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
3301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
3361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
3421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
3481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
3541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
3601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
3661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
3721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
3781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
3841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
3901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
3961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
4021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
4081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
4141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
4201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
4261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
4321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
4381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
4441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
4501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
4561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
4621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
4681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
4741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
4801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
4861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
4921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
4981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
5041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
5101 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
5161 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
5221 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
5281 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
5341 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
5401 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
5461 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
5521 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
5581 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
5641 ctattacgcc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt
5701 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
5761 aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta ct
//
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