| 质粒名称: | Circular-oc-CVB3-GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 226262 |
| 存储实验室: | Prashant Mali |
|
载体骨架基本信息 |
|
| 载体名称: | 未知 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 6350bp |
| 载体类型: | In Vitro Transcription |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | EGFP |
| 物种来源: | Synthetic |
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: | Circular-oc-CVB3-GFP was a gift from Prashant Mali (Addgene plasmid # 226262 ; http://n2t.net/addgene:226262 ; RRID:Addgene_226262) |
| 参考文献部分: | Robust genome and cell engineering via in vitro and in situ circularized RNAs. Tong M, Palmer N, Dailamy A, Kumar A, Khaliq H, Han S, Finburgh E, Wing M, Hong C, Xiang Y, Miyasaki K, Portell A, Rainaldi J, Suhardjo A, Nourreddine S, Chew WL, Kwon EJ, Mali P. Nat Biomed Eng. 2024 Aug 26. doi: 10.1038/s41551-024-01245-z. 10.1038/s41551-024-01245-z PubMed |
买家导航
Circular-oc-CVB3-GFP载体质粒基本信息
Circular-oc-CVB3-GFP质粒图谱载体图谱和Circular-oc-CVB3-GFP载体序列质粒序列多克隆位点信息
Circular-oc-CVB3-GFP质粒载体简介
Circular-oc-CVB3-GFP质粒序列载体序列
LOCUS Circular-oc-CVB3-GFP 6350 bp DNA circular SYN 15-DEC-2025
DEFINITION Production of in vitro circularizable RNA via IVT.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6350)
AUTHORS Tong M, Palmer N, Dailamy A, Kumar A, Khaliq H, Han S, Finburgh E,
Wing M, Hong C, Xiang Y, Miyasaki K, Portell A, Rainaldi J, Suhardjo
A, Nourreddine S, Chew WL, Kwon EJ, Mali P
TITLE Robust genome and cell engineering via in vitro and in situ
circularized RNAs.
JOURNAL Nat Biomed Eng. 2024 Aug 26. doi: 10.1038/s41551-024-01245-z.
PUBMED 39187662
REFERENCE 2 (bases 1 to 6350)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed
Eng. 2024 Aug 26. doi: 10.1038/s41551-024-01245-z."
COMMENT Sequence Label: Circular-oc-CVB3-GFP
FEATURES Location/Qualifiers
source 1..6350
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(142..161)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 955..1543
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1444..1463
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
protein_bind 1831..1852
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(1867..1872,1873..1890,1891..1897)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1905..1921
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1910..1932
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 1929..1945
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1929..1945
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
repeat_region 1983..2112
/label=AAV2 ITR
primer_bind 2197..2216
/label=T7
/note="T7 promoter, forward primer"
promoter 2197..2215
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS join(3156..3158,3159..3161,3162..3875)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(3156..3158,3159..3161,3162..3872)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3201..3222)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3462..3481)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3809..3830
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(3935..3955)
/label=WPRE-R
/note="WPRE, reverse primer"
repeat_region 5382..5511
/label=AAV2 ITR
primer_bind complement(5530..5547)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5530..5546)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5539..5561)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 5688..6143
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5775..5794)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5985..6006
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6169..6273
/gene="bla"
/label=AmpR promoter
CDS join(6274..6342,6343..6350,1..784)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
61 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
121 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
181 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
241 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
301 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
361 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
421 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
481 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
541 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
601 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
661 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
721 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
781 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
841 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
901 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
961 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
1021 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
1081 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
1141 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
1201 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
1261 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
1321 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
1381 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
1441 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
1501 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
1561 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
1621 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
1681 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
1741 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
1801 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
1861 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
1921 atttcacaca ggaaacagct atgaccatga ttacgccaga tttaattaag gccttaatta
1981 ggctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct
2041 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca
2101 ctaggggttc cttgtagtta atgattaacc cgccatgcta cttatctacg tagccatgct
2161 ctaggaagat cggaattcgc ccttaagcta gcatgctaat acgactcact atagggccat
2221 cagtcgccgg tcccaagccc ggataaaatg ggagggggcg ggaaaccgcc taaccatgcc
2281 gactgatggc agctattatc gagcgaacgc cttatgcgat gaaagtcgca cgtagggtgt
2341 agaccaagcg aaatcctatg catttaggat agtgaggtat agcaaaggag aagtcgacgg
2401 gcccgcggaa ttccttaaaa cagcctgtgg gttgatccca cccacaggcc cattgggcgc
2461 tagcactctg gtatcacggt acctttgtgc gcctgtttta taccccctcc cccaactgta
2521 acttagaagt aacacacacc gatcaacagt cagcgtggca caccagccac gttttgatca
2581 agcacttctg ttaccccgga ctgagtatca atagactgct cacgcggttg aaggagaaag
2641 cgttcgttat ccggccaact acttcgaaaa acctagtaac accgtggaag ttgcagagtg
2701 tttcgctcag cactacccca gtgtagatca ggtcgatgag tcaccgcatt ccccacgggc
2761 gaccgtggcg gtggctgcgt tggcggcctg cccatgggga aacccatggg acgctctaat
2821 acagacatgg tgcgaagagt ctattgagct agttggtagt cctccggccc ctgaatgcgg
2881 ctaatcctaa ctgcggagca cacaccctca agccagaggg cagtgtgtcg taacgggcaa
2941 ctctgcagcg gaaccgacta ctttgggtgt ccgtgtttca ttttattcct atactggctg
3001 cttatggtga caattgagag atcgttacca tatagctatt ggattggcca tccggtgact
3061 aatagagcta ttatatatcc ctttgttggg tttataccac ttagcttgaa agaggttaaa
3121 acattacaat tcattgttaa gttgaataca gcaaaatggt gagcaagggc gaggagctgt
3181 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
3241 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
3301 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
3361 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
3421 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
3481 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
3541 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
3601 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
3661 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
3721 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
3781 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
3841 ggatcactct cggcatggac gagctgtaca agtaaggatc caatcaacct ctggattaca
3901 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
3961 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
4021 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
4081 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
4141 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
4201 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
4261 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
4321 ttctgcgcgg gacgtccttc tgctacaatc cagcggacct tccttcccgc ggcctgctgc
4381 cggctctgcg gcctcttccg cgtcttcgaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
4441 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
4501 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
4561 aaaaaaaaaa aaaaaactat agccatacaa taaaagtgcg aaacgttatc ctataagtaa
4621 gaaagtttta aaattttctt acgaaaagga tagaacttaa aagttctaac tgttctacta
4681 aagtaataag tgaaaatctt atttaaagca aacaaccaag tagctttaag tctaagtccc
4741 ctacacaagt tttatactac tatgcaaaac ttgtgaagct aggtaaggtc gtaatccgtg
4801 aaagtcggat gcggggctcc ttaaaagatt actatggtaa acataagcta atccattaag
4861 atgcgattta tatgtatttt atactgttaa atatttttgt gcttgtggct tggtataaaa
4921 cagttaagat gaagtactta actggttttg gaataattgg ttgttaaact aaaacattat
4981 aaatcgttag tggataccta aggtaatcaa aaatagggat aggtagaatg gaacgtttga
5041 tgctgtatat gaagaggttt agtagaacct aggacacata tacgggctca gcaggttcat
5101 agtagctatg atactcagcc ggaagtcaaa ttaattttga aatacttcta tggtaacata
5161 ggagaaggat aaaactgagt gagccaagga acctagtcgg taatagctgc catcagtcgg
5221 cgtggactgt agaacactgc caatgccggt cccaagcccg gataaaagtg gagggtacag
5281 tccacgcttt ttttctcgag ttaagggcga attcccgata aggatcttcc tagagcatgg
5341 ctacgtagat aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga
5401 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
5461 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gccttaatta
5521 acctaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
5581 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
5641 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
5701 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
5761 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
5821 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
5881 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
5941 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
6001 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
6061 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
6121 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
6181 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
6241 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
6301 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
//
