| 质粒名称: | pYK657 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000835 |
| 产品用途: | Mammalian PhCMV-driven constitutive 3xGNCR-NLS-MCP expression vector (PhCMV-3xGNCR-NLS-MCP-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | 3xGNCR-NLS-MCP |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK657
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK657载体质粒基本信息
pYK657质粒图谱载体图谱和pYK657载体序列质粒序列多克隆位点信息
pYK657质粒载体简介
pYK657质粒序列载体序列
LOCUS pYK657 8088 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8088)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8088
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..946
/label=oYF0019
primer_bind 881..918
/label=oYK421_GNCR_F
primer_bind 881..902
/label=oYK31-mCherry-NS3a-F
primer_bind 881..900
/label=oYK57-NLS-F
primer_bind 910..952
/label=oYF005
CDS 919..1617
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 919..952
/label=oYF0011
primer_bind 919..943
/label=oLZ146 GNCR1-N F
primer_bind 919..943
/label=oSCL762
primer_bind 1585..1597
/label=oYK03-02-F
primer_bind 1585..1596
/label=oYK31-mCherry-NS3a-F
primer_bind complement(1596..1652)
/label=oYF0020
primer_bind complement(1596..1647)
/label=oYF006
primer_bind complement(1596..1618)
/label=oYF0012
primer_bind 1615..1637
/label=oSCL1129
primer_bind 1615..1635
/label=oLZ147 NS3a F
primer_bind 1615..1634
/label=oYF0033
primer_bind 1617..1637
/label=oLZ048 NSP3-F
primer_bind 1617..1634
/label=oYK419_GS_F
primer_bind 1618..1647
/label=oSCL388
primer_bind 1618..1639
/label=oYK1256_MCP_F
primer_bind 1618..1633
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1619..1652)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(1625..1643)
/label=Oscl18-4
primer_bind complement(1628..1648)
/label=oSCL1119
primer_bind complement(1628..1647)
/label=oSCL075
primer_bind complement(1628..1647)
/label=oSCL075a
primer_bind complement(1628..1647)
/label=oSCL075`b
primer_bind 1630..1686
/label=oYK1256_MCP_F
primer_bind complement(1631..1647)
/label=oYK472_NLS_R
primer_bind 1632..1678
/label=oLZ146 GNCR1-N F
primer_bind complement(1632..1648)
/label=oYK92_L7Ae_R
misc_feature 1634..1652
/label=linker
primer_bind complement(1636..1648)
/label=oYK75_NLS_mcp_R
CDS 1654..2352
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 1654..1687
/label=oYF005
primer_bind 1654..1687
/label=oYF0011
primer_bind 1654..1681
/label=oYF0019
primer_bind 1654..1678
/label=oSCL762
primer_bind 2320..2332
/label=oYK03-02-F
primer_bind 2320..2331
/label=oYK31-mCherry-NS3a-F
primer_bind complement(2331..2387)
/label=oYF0020
primer_bind complement(2331..2382)
/label=oYF006
primer_bind complement(2331..2353)
/label=oYF0012
primer_bind 2350..2372
/label=oSCL1129
primer_bind 2350..2370
/label=oLZ147 NS3a F
primer_bind 2350..2369
/label=oYF0033
primer_bind 2352..2372
/label=oLZ048 NSP3-F
primer_bind 2352..2369
/label=oYK419_GS_F
primer_bind 2353..2382
/label=oSCL388
primer_bind 2353..2374
/label=oYK1256_MCP_F
primer_bind 2353..2368
/label=oYK28-mCherry-NS3a-F
primer_bind complement(2354..2387)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(2360..2378)
/label=Oscl18-4
primer_bind complement(2363..2383)
/label=oSCL1119
primer_bind complement(2363..2382)
/label=oSCL075
primer_bind complement(2363..2382)
/label=oSCL075a
primer_bind complement(2363..2382)
/label=oSCL075`b
primer_bind 2365..2421
/label=oYK1256_MCP_F
primer_bind complement(2366..2382)
/label=oYK472_NLS_R
primer_bind 2367..2413
/label=oLZ146 GNCR1-N F
primer_bind complement(2367..2383)
/label=oYK92_L7Ae_R
misc_feature 2369..2387
/label=linker
primer_bind complement(2371..2383)
/label=oYK75_NLS_mcp_R
CDS 2389..3087
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 2389..2422
/label=oYF005
primer_bind 2389..2422
/label=oYF0011
primer_bind 2389..2416
/label=oYF0019
primer_bind 2389..2413
/label=oSCL762
primer_bind 3055..3067
/label=oYK03-02-F
primer_bind 3055..3066
/label=oYK31-mCherry-NS3a-F
primer_bind complement(3066..3123)
/label=oYF0020
primer_bind complement(3066..3117)
/label=oYF006
primer_bind complement(3066..3088)
/label=oYF0012
primer_bind 3085..3107
/label=oSCL1129
primer_bind 3085..3105
/label=oLZ147 NS3a F
primer_bind 3085..3104
/label=oYF0033
primer_bind 3087..3107
/label=oLZ048 NSP3-F
primer_bind 3087..3104
/label=oYK419_GS_F
primer_bind 3088..3117
/label=oSCL388
primer_bind 3088..3109
/label=oYK1256_MCP_F
primer_bind 3088..3103
/label=oYK28-mCherry-NS3a-F
primer_bind complement(3089..3137)
/label=oLZ145 DNCR2-GNCR1 R
primer_bind complement(3095..3113)
/label=Oscl18-4
primer_bind complement(3098..3154)
/label=oSCL1119
primer_bind complement(3098..3117)
/label=oSCL075
primer_bind complement(3098..3117)
/label=oSCL075a
primer_bind complement(3098..3117)
/label=oSCL075`b
primer_bind complement(3101..3117)
/label=oYK472_NLS_R
primer_bind 3102..3122
/label=oLZ146 GNCR1-N F
primer_bind complement(3102..3118)
/label=oYK92_L7Ae_R
misc_feature 3104..3123
/label=linker
primer_bind complement(3106..3118)
/label=oYK75_NLS_mcp_R
primer_bind complement(3115..3138)
/label=oYK421_GNCR3_R
primer_bind 3119..3156
/label=oYK648_NLS_F
misc_feature 3124..3138
/label=NSP3
primer_bind 3138..3222
/label=oYK57-NLS-F
CDS 3138..3195
/codon_start=1
/label=NLS
/translation="PKKKRKVEASASPKKKRKV"
primer_bind 3176..3197
/label=oYK419_GS_F
CDS 3196..3582
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
primer_bind complement(3558..3606)
/label=oYK432_MCP_R
primer_bind complement(3562..3591)
/label=Oscl32-2
primer_bind complement(3562..3582)
/label=oSCL093
primer_bind complement(3582..3608)
/label=oYK472_NLS_R
primer_bind complement(3586..3608)
/label=oYK57-MCP-mCherry-R
terminator 3682..3909
/label=bGH PA
misc_feature 3955..4383
/label=f1 origin
misc_feature 4388..4731
/label=SV40 early promoter and origin
misc_feature 4577..4654
/label=SV40 ORI
CDS 4796..5587
/label=Kan/neoR
misc_feature 5761..5891
/label=SV40 early polyadenylation signal
primer_bind complement(5927..5947)
/label=M13-rev
misc_binding complement(5953..5975)
/label=LacO
promoter complement(5980..6009)
/label=lac
misc_feature complement(6274..6947)
/label=pUC origin
CDS complement(7095..7754)
/label=AmpR
promoter complement(7994..8022)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gctggctagg ccaccatgga catcgagaag ctgtgcaaga aggcggaaga ggaggccaag
961 gaggcccagg agaaggccga cgagctgaga caacgacacc ccgattcgca ggcggcggaa
1021 gacgcggagg atctcgcaaa tctggctgtc gccgccgtgc tgaccgcttg tctgctggcc
1081 caggagcatc ccaacgccga tatcgctaag ctgtgcatca aggccgcgag cgaagctgcg
1141 gaggccgcgt caaaagccgc cgagctggcc caacggcacc cagacagtca agcagccaga
1201 gatgctatca aactcgcctc tcaagccgcc cgcgccgtga tcctggccat catgctcgcc
1261 gcagagaatc ccaacgctga tattgcaaag ctatgtatta aggcggccag cgaagccgca
1321 gaggctgcct cgaaagctgc ggaacttgca cagcggcacc ccgactccca ggccgcccga
1381 gatgcaatta aactggccag tcaggccgct gaggcggtgg agagagccat ctggctggcc
1441 gccgagaacc ccaatgccga tatcgccaaa aaatgcatca aagcggcctc cgaagcagcc
1501 gaggaggcct ctaaagctgc cgaagaggct caaagacacc cagacagcca gaaggcacgc
1561 gacgagatca aggaggcctc tcagaaggcc gaggaggtga aggagagatg caagagcgcc
1621 agcggaagtg gcggaggagg cgacgtcgct agtgacatcg agaagctgtg caagaaggcg
1681 gaagaggagg ccaaggaggc ccaggagaag gccgacgagc tgagacaacg acaccccgat
1741 tcgcaggcgg cggaagacgc ggaggatctc gcaaatctgg ctgtcgccgc cgtgctgacc
1801 gcttgtctgc tggcccagga gcatcccaac gccgatatcg ctaagctgtg catcaaggcc
1861 gcgagcgaag ctgcggaggc cgcgtcaaaa gccgccgagc tggcccaacg gcacccagac
1921 agtcaagcag ccagagatgc tatcaaactc gcctctcaag ccgcccgcgc cgtgatcctg
1981 gccatcatgc tcgccgcaga gaatcccaac gctgatattg caaagctatg tattaaggcg
2041 gccagcgaag ccgcagaggc tgcctcgaaa gctgcggaac ttgcacagcg gcaccccgac
2101 tcccaggccg cccgagatgc aattaaactg gccagtcagg ccgctgaggc ggtggagaga
2161 gccatctggc tggccgccga gaaccccaat gccgatatcg ccaaaaaatg catcaaagcg
2221 gcctccgaag cagccgagga ggcctctaaa gctgccgaag aggctcaaag acacccagac
2281 agccagaagg cacgcgacga gatcaaggag gcctctcaga aggccgagga ggtgaaggag
2341 agatgcaaga gcgccagcgg aagtggcgga ggaggcgacg tcgctagtga catcgagaag
2401 ctgtgcaaga aggcggaaga ggaggccaag gaggcccagg agaaggccga cgagctgaga
2461 caacgacacc ccgattcgca ggcggcggaa gacgcggagg atctcgcaaa tctggctgtc
2521 gccgccgtgc tgaccgcttg tctgctggcc caggagcatc ccaacgccga tatcgctaag
2581 ctgtgcatca aggccgcgag cgaagctgcg gaggccgcgt caaaagccgc cgagctggcc
2641 caacggcacc cagacagtca agcagccaga gatgctatca aactcgcctc tcaagccgcc
2701 cgcgccgtga tcctggccat catgctcgcc gcagagaatc ccaacgctga tattgcaaag
2761 ctatgtatta aggcggccag cgaagccgca gaggctgcct cgaaagctgc ggaacttgca
2821 cagcggcacc ccgactccca ggccgcccga gatgcaatta aactggccag tcaggccgct
2881 gaggcggtgg agagagccat ctggctggcc gccgagaacc ccaatgccga tatcgccaaa
2941 aaatgcatca aagcggcctc cgaagcagcc gaggaggcct ctaaagctgc cgaagaggct
3001 caaagacacc cagacagcca gaaggcacgc gacgagatca aggaggcctc tcagaaggcc
3061 gaggaggtga aggagagatg caagagcgcc agcggaagtg gcggaggagg cgacgtcgct
3121 agcatgctca agatggagcc caagaagaag cgcaaggtgg aagctagcgc ttccccgaag
3181 aaaaagcgga aagtcgcttc aaactttact cagttcgtgc tcgtggacaa tggtgggaca
3241 ggggatgtga cagtggctcc ttctaatttc gctaatgggg tggcagagtg gatcagctcc
3301 aactcacgga gccaggccta caaggtgaca tgcagcgtca ggcagtctag tgcccagaag
3361 agaaagtata ccatcaaggt ggaggtcccc aaagtggcta cccagacagt gggcggagtc
3421 gaactgcctg tcgccgcttg gaggtcctac ctgaacatgg agctcactat cccaattttc
3481 gctaccaatt ctgactgtga actcatcgtg aaggcaatgc aggggctcct caaagacggt
3541 aatcctatcc cttccgccat cgccgctaac tcaggtatct actaaggatc cactagtcca
3601 gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg
3661 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
3721 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
3781 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
3841 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
3901 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
3961 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
4021 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
4081 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
4141 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
4201 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
4261 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
4321 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
4381 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
4441 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
4501 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
4561 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
4621 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
4681 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
4741 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
4801 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
4861 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
4921 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
4981 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
5041 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
5101 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
5161 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
5221 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
5281 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
5341 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
5401 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
5461 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
5521 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
5581 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
5641 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
5701 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
5761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
5821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
5881 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
5941 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
6001 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
6061 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
6121 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
6181 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
6241 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
6301 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
6361 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
6421 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
6481 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
6541 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
6601 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
6661 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
6721 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
6781 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
6841 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
6901 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
6961 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
7021 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
7081 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
7141 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
7201 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
7261 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
7321 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
7381 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
7441 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
7501 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
7561 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
7621 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
7681 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
7741 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
7801 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
7861 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
7921 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
7981 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
8041 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
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