| 质粒名称: | T7-VEE-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 58977 |
| 存储实验室: | Steven Dowdy |
|
载体骨架基本信息 |
|
| 载体名称: | T7-VEE-IRES-puro (Addgene plasmid 58970) |
| 载体出品公司: | Dowdy lab |
| 载体抗性: | 氨苄青霉素 |
| 载体类型: | 哺乳动物表达；委内瑞拉马脑炎（VEE）病毒RNA复制子 |
| 筛选标记: | 嘌呤霉素 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EGFP |
| 物种来源: | Aequorea victoria |
| 启动子 | 26S subgenomic promoter |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI (未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 3' 测序引物 | pCDH-rev (GCATTCCTTTGGCGAGAG) |
|
文献引用方法 |
|
| 材料和方法部分: | T7-VEE-GFP was a gift from Steven Dowdy (Addgene plasmid # 58977 ; http://n2t.net/addgene:58977 ; RRID:Addgene_58977) |
| 参考文献部分: | Efficient generation of human iPSCs by a synthetic self-replicative RNA. Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. 10.1016/j.stem.2013.06.001 PubMed 23910086 |
买家导航
T7-VEE-GFP载体质粒基本信息
T7-VEE-GFP质粒图谱载体图谱和T7-VEE-GFP载体序列质粒序列多克隆位点信息
T7-VEE-GFP质粒载体简介
T7-VEE-GFP质粒序列载体序列
LOCUS T7-VEE-GFP 11522 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION expression of GFP using a self-replicating Venezuelan equine
encephalitis (VEE) virus RNA replicon.
ACCESSION .
VERSION .
KEYWORDS T7-VEE-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11522)
AUTHORS Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR,
Chi NC, Fu XD, Yu BD, Dowdy SF
TITLE Efficient generation of human iPSCs by a synthetic self-replicative
RNA.
JOURNAL Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi:
10.1016/j.stem.2013.06.001.
PUBMED 23910086
REFERENCE 2 (bases 1 to 11522)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11522
/organism="synthetic DNA construct"
/mol_type="other DNA"
RBS 1535..1543
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
regulatory 7600..7609
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 7606..8322
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(7651..7672)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(7912..7931)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 8259..8280
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(8554..8571)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 8781..8800
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 8962..9561
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(8962..8981)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 9458..9478
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
promoter 9760..9864
/gene="bla"
/label=AmpR promoter
CDS 9865..10725
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10083..10102)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10896..11484
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11385..11404
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 11505..11522
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg
61 ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg
121 aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc
181 tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa
241 gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat
301 gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg
361 aaataactga taaggaattg gacaagaaaa tgaaggagct ggccgccgtc atgagcgacc
421 ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc
481 aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag
541 ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta
601 agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa
661 cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt
721 ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga
781 ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact
841 tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg
901 tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta
961 cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg
1021 tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac
1081 tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta
1141 tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg
1201 tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa
1261 ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc
1321 acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg
1381 atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa
1441 caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg
1501 acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt
1561 tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaggcagacg
1621 tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa
1681 aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg
1741 ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga
1801 taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg
1861 tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca
1921 ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag
1981 gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg
2041 aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag
2101 ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa
2161 cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag
2221 gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga
2281 aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg
2341 ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata
2401 ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac
2461 ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc
2521 tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc
2581 gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa
2641 cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc
2701 aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca
2761 aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg
2821 ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg
2881 tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga
2941 taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag
3001 cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc
3061 agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca
3121 tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact
3181 cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg
3241 gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc
3301 cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc
3361 cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc
3421 gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag
3481 tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg
3541 gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt
3601 tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg
3661 tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc
3721 agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc
3781 tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa
3841 gcatcattgg tgctatagcg cggcagttca agttttcccg ggtatgcaaa ccgaaatcct
3901 cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc
3961 acaattctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg
4021 aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag
4081 gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc
4141 tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac
4201 tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt
4261 cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca
4321 acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga
4381 acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg
4441 cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg
4501 ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg
4561 atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca
4621 caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg
4681 atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca
4741 tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg
4801 aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa
4861 gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat
4921 tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct
4981 caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag
5041 acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac
5101 cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg
5161 aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg
5221 aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat
5281 ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca
5341 gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc
5401 gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa
5461 gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc
5521 caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc
5581 ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga
5641 ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg
5701 catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa
5761 cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc
5821 tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta
5881 acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta
5941 ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc
6001 tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg
6061 cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta
6121 ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca
6181 ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac
6241 ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag
6301 ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg
6361 cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt
6421 ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa
6481 aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca
6541 taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa
6601 aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag
6661 cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga
6721 acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact
6781 tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg
6841 acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt
6901 tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta
6961 aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag
7021 tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg
7081 cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag
7141 acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga
7201 aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc
7261 gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg
7321 aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg
7381 gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca
7441 tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag
7501 gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa
7561 gtctagcata tgggcgcgcc ctcagcatcg attcaattcg ccaccatggt gagcaagggc
7621 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
7681 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
7741 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
7801 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
7861 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
7921 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
7981 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
8041 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
8101 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
8161 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
8221 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
8281 accgccgccg ggatcactct cggcatggac gagctgtaca agtagtctag agtcgacccg
8341 ggcggccgca actaacttaa gctagcaacg gtttccctct agcgggatca attccgcccc
8401 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
8461 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
8521 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
8581 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
8641 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
8701 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
8761 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
8821 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
8881 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
8941 tttgaaaaac acgataatac catgaccgag tacaagccca cggtgcgcct cgccacccgc
9001 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
9061 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
9121 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
9181 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
9241 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
9301 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
9361 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
9421 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
9481 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
9541 acccgcaagc ccggtgcctg agaattggca agctgcttac atagaactcg cggcgattgg
9601 catgccgcct taaaattttt attttatttt ttcttttctt ttccgaatcg gattttgttt
9661 ttaatatttc aaaaaaaaaa aaaaaaaaaa aaaaaacgcg tcgaggggaa ttaattcttg
9721 aagacgaaag ggccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
9781 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
9841 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
9901 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
9961 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
10021 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
10081 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
10141 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
10201 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
10261 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
10321 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
10381 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
10441 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
10501 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
10561 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
10621 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
10681 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
10741 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
10801 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
10861 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
10921 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
10981 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
11041 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
11101 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
11161 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
11221 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
11281 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
11341 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
11401 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
11461 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcga gctctaatac gactcactat
11521 ag
//
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T7-VEE-GFP质粒载体应用举例
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