| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-FLNA-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001754 |
| 产品用途: | 3' AAVLINK plasmid for FLNA expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | FLNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-FLNA-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-FLNA-C-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-FLNA-C-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-FLNA-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-FLNA-C-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-FLNA-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-FLNA-C-FLAG 6914 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6914)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6914
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3627
/codon_start=1
/label=FLNA
/translation="VTTPCVCPT*RSALLPTSPSTSQRRISAC*RPLWSRPRAGRSPVC
*SGCVMATWGFHSCPRRRGSTWCM*RKMASTWPAAPSRW*SASRKLGMPVVFGSLVRAF
TKATPLSLQSLSLIPAMQAMVGSACPLRAPARWTSTQRTWRTGRAGSPTAPQSQATTSS
TSSLPTSTCLAAPSL*R*QARAG*KRASPAGVGLLQWPTLVVIVTSA*KSLKLASRI*Q
PR*PAHRARPMRPRSWKGRTTPTASALFPLRWAHTQSA*STRASTCLGAPSSSPWGP*G
KGEPTRSELGALAWRELKLECQPNSVSGPGKLVLEAWPLLSRAPARLRSLLRTARTAPV
VWLMWSRSQVTTKSQSSSTRNTFPTAPSWCLWLLRLATPAASLFLAFRSQG*RSTSQPL
LQSA*TGPRGRSMPRCTAPQEPWRSAMSQKLTKISMLCASSLGRMAFT*LTSSSTAPTS
LEAPSRSELGSLGMEGTQAWCLLTEQVWKAVSQGTQLSSS*TRAMRELVPCR*PLTAPP
R*RWIARSALRATASPIPPWHLAATSSPSSTAAPTTLGAAPSRPKSQAPVSSATTASTR
HHQCL*TL*PRPPVPPSMGPRVLGLLTPARWWPRAWG*ARPT*ARRAASQ*TAAKQATT
CCWWGFMAQGPPARRSW*STWAAGSTACPTCSRTRGSTHWWSNGGTSTSQAAPTALWC"
CDS 3634..3699
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3703..4134
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3847..3852
/label=SDS
repeat_region 4177..4317
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4177..4306
/label=AAV2 ITR
rep_origin 4392..4847
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5129..5233
/gene="bla"
/label=AmpR promoter
CDS 5234..6094
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6265..6853
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtga cgactccatg
1561 cgtatgtccc acctaaaggt cggctctgct gccgacatcc ccatcaacat ctcagagacg
1621 gatctcagcc tgctgacggc cactgtggtc ccgccctcgg gccgggagga gccctgtttg
1681 ctgaagcggc tgcgtaatgg ccacgtgggg atttcattcg tgcccaagga gacgggggag
1741 cacctggtgc atgtgaagaa aaatggccag cacgtggcca gcagccccat cccggtggtg
1801 atcagccagt cggaaattgg ggatgccagt cgtgttcggg tctctggtca gggccttcac
1861 gaaggccaca cctttgagcc tgcagagttt atcattgata cccgcgatgc aggctatggt
1921 gggctcagcc tgtccattga gggccccagc aaggtggaca tcaacacaga ggacctggag
1981 gacgggacgt gcagggtcac ctactgcccc acagagccag gcaactacat catcaacatc
2041 aagtttgccg accagcacgt gcctggcagc cccttctctg tgaaggtgac aggcgagggc
2101 cgggtgaaag agagcatcac ccgcaggcgt cgggctcctt cagtggccaa cgttggtagt
2161 cattgtgacc tcagcctgaa aatccctgaa attagcatcc aggatatgac agcccaggtg
2221 accagcccat cgggcaagac ccatgaggcc gagatcgtgg aaggggagaa ccacacctac
2281 tgcatccgct ttgttcccgc tgagatgggc acacacacag tcagcgtgaa gtacaagggc
2341 cagcacgtgc ctgggagccc cttccagttc accgtggggc ccctagggga agggggagcc
2401 cacaaggtcc gagctggggg ccctggcctg gagagagctg aagctggagt gccagccgaa
2461 ttcagtatct ggacccggga agctggtgct ggaggcctgg ccattgctgt cgagggcccc
2521 agcaaggctg agatctcttt tgaggaccgc aaggacggct cctgtggtgt ggcttatgtg
2581 gtccaggagc caggtgacta cgaagtctca gtcaagttca acgaggaaca cattcccgac
2641 agccccttcg tggtgcctgt ggcttctccg tctggcgacg cccgccgcct cactgtttct
2701 agccttcagg agtcagggct aaaggtcaac cagccagcct cttttgcagt cagcctgaac
2761 ggggccaagg gggcgatcga tgccaaggtg cacagcccct caggagccct ggaggagtgc
2821 tatgtcacag aaattgacca agataagtat gctgtgcgct tcatccctcg ggagaatggc
2881 gtttacctga ttgacgtcaa gttcaacggc acccacatcc ctggaagccc cttcaagatc
2941 cgagttgggg agcctgggca tggaggggac ccaggcttgg tgtctgctta cggagcaggt
3001 ctggaaggcg gtgtcacagg gaacccagct gagttcgtcg tgaacacgag caatgcggga
3061 gctggtgccc tgtcggtgac cattgacggc ccctccaagg tgaagatgga ttgccaggag
3121 tgccctgagg gctaccgcgt cacctatacc cccatggcac ctggcagcta cctcatctcc
3181 atcaagtacg gcggccccta ccacattggg ggcagcccct tcaaggccaa agtcacaggc
3241 ccccgtctcg tcagcaacca cagcctccac gagacatcat cagtgtttgt agactctctg
3301 accaaggcca cctgtgcccc ccagcatggg gccccgggtc ctgggcctgc tgacgccagc
3361 aaggtggtgg ccaagggcct ggggctgagc aaggcctacg taggccagaa gagcagcttc
3421 acagtagact gcagcaaagc aggcaacaac atgctgctgg tgggggttca tggcccaagg
3481 accccctgcg aggagatcct ggtgaagcac gtgggcagcc ggctctacag cgtgtcctac
3541 ctgctcaagg acaaggggga gtacacactg gtggtcaaat ggggggacga gcacatccca
3601 ggcagcccct accgcgttgt ggtgccctgt acagactaca aagaccatga cggtgattat
3661 aaagatcatg acatcgacta caaggatgac gatgacaagt aactcgagat aatcaacctc
3721 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
3781 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
3841 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
3901 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta
3961 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt
4021 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
4081 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct
4141 ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
4201 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
4261 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
4321 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
4381 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
4441 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
4501 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
4561 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
4621 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
4681 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
4741 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
4801 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
4861 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
4921 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
4981 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
5041 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
5101 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
5161 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
5221 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
5281 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
5341 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
5401 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
5461 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
5521 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
5581 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
5641 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
5701 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
5761 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
5821 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
5881 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
5941 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
6001 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
6061 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
6121 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
6181 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
6241 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
6301 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
6361 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
6421 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
6481 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
6541 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
6601 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
6661 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
6721 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
6781 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6841 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6901 ttttgctcac atgt
//
