| 质粒名称: | pYK57 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000784 |
| 产品用途: | Mammalian PhCMV-driven constitutive NLS-MCP-mCherry expression vector (PhCMV-NLS-MCP-mCherry-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: |
NLS-MCP-mCherry
|
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK57
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK57载体质粒基本信息
pYK57质粒图谱载体图谱和pYK57载体序列质粒序列多克隆位点信息
pYK57质粒载体简介
pYK57质粒序列载体序列
LOCUS pYK57 6598 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6598)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Jul 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6598
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..991
/label=oYK57-NLS-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
misc_feature 905..964
/label=NLS
CDS 965..1351
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
primer_bind 965..982
/label=oYK05-mcp-F
primer_bind complement(1331..1381)
/label=oSCL093
primer_bind complement(1331..1351)
/label=Oscl32-2
primer_bind complement(1351..1377)
/label=oDJ171-4
primer_bind 1351..1371
/label=oLZ048 NSP3-F
primer_bind 1352..1411
/label=oDJ171-3
misc_feature 1352..1387
/label=linker
misc_feature 1352..1386
/label=linker
primer_bind 1352..1381
/label=oSCL388
primer_bind 1352..1371
/label=oSCL1192
primer_bind 1352..1369
/label=oLZ147 NS3a F
primer_bind 1352..1368
/label=oYF0033
primer_bind complement(1362..1399)
/label=oSCL1119
primer_bind complement(1362..1381)
/label=oSCL075
primer_bind complement(1370..1397)
/label=oYK05-mcp-R
primer_bind 1372..1406
/label=oYK03-02-F
primer_bind 1378..1405
/label=oYK05-mcherry-F
primer_bind 1382..1414
/label=omz_linker_mCherry_F
CDS 1388..2092
/codon_start=1
/label=mCherry
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind 1388..1408
/label=oXQ135
primer_bind 1388..1408
/label=oXQ174
primer_bind 1388..1408
/label=omz_mCherry_F
primer_bind 1388..1405
/label=oFP01-mCherry_F
primer_bind 1388..1405
/label=oYK284_mCherry_F
misc_feature 1388..1390
/label=Coh2
primer_bind complement(1700..1711)
/label=oSCL011
primer_bind complement(1700..1711)
/label=omz_Coh2_R
primer_bind complement(1700..1711)
/label=omz_Coh2_His_R
primer_bind complement(2072..2136)
/label=oXQ161
primer_bind complement(2072..2116)
/label=oXP01_mCherry_R
primer_bind complement(2072..2115)
/label=oYK05-mcherry-R
primer_bind complement(2072..2100)
/label=oXQ136
primer_bind complement(2072..2092)
/label=oDJ171-4
primer_bind complement(2072..2092)
/label=omz_mCherry_His_R
primer_bind complement(2074..2092)
/label=omz_mCherry_linker_R
primer_bind complement(2096..2118)
/label=oYK57-MCP-mCherry-R
terminator 2192..2419
/label=bGH PA
misc_feature 2465..2893
/label=f1 origin
misc_feature 2898..3241
/label=SV40 early promoter and origin
misc_feature 3087..3164
/label=SV40 ORI
CDS 3306..4097
/label=Kan/neoR
misc_feature 4271..4401
/label=SV40 early polyadenylation signal
primer_bind complement(4437..4457)
/label=M13-rev
misc_binding complement(4463..4485)
/label=LacO
promoter complement(4490..4519)
/label=lac
misc_feature complement(4784..5457)
/label=pUC origin
CDS complement(5605..6264)
/label=AmpR
promoter complement(6504..6532)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
961 agtcgcttca aactttactc agttcgtgct cgtggacaat ggtgggacag gggatgtgac
1021 agtggctcct tctaatttcg ctaatggggt ggcagagtgg atcagctcca actcacggag
1081 ccaggcctac aaggtgacat gcagcgtcag gcagtctagt gcccagaaga gaaagtatac
1141 catcaaggtg gaggtcccca aagtggctac ccagacagtg ggcggagtcg aactgcctgt
1201 cgccgcttgg aggtcctacc tgaacatgga gctcactatc ccaattttcg ctaccaattc
1261 tgactgtgaa ctcatcgtga aggcaatgca ggggctcctc aaagacggta atcctatccc
1321 ttccgccatc gccgctaact caggtatcta cgccagcgga agtggcggag gaggcgacgt
1381 cggatccgtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
1441 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
1501 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
1561 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
1621 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
1681 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
1741 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
1801 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
1861 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
1921 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
1981 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
2041 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaggatc
2101 cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct
2161 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
2221 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
2281 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
2341 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
2401 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
2461 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
2521 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
2581 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
2641 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
2701 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
2761 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
2821 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
2881 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
2941 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
3001 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
3061 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
3121 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
3181 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
3241 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
3301 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
3361 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
3421 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
3481 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
3541 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
3601 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
3661 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
3721 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
3781 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
3841 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
3901 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
3961 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
4021 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
4081 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
4141 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
4201 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
4261 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
4321 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
4381 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
4441 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
4501 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
4561 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
4621 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
4681 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
4741 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
4801 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
4861 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
4921 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
4981 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
5041 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
5101 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
5161 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
5221 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
5281 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
5341 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
5401 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
5461 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
5521 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
5581 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
5641 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
5701 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
5761 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
5821 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
5881 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
5941 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
6001 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
6061 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
6121 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
6181 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
6241 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
6301 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
6361 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
6421 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
6481 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
6541 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
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