| 质粒名称: | pGL3-ERCC6-hCtRNA-Nicking/PegRNA-PGK-puroR |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002193 |
| 产品用途: | The hCtRNA simultaneously drives the expression of both the targeting ERCC6 gene NickingRNA and pegRNA, harboring puroR selection marker. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | hCtRNA |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
ERCC6-nicking-sgRNA-pegRNA
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-ERCC6-hCtRNA-Nicking/PegRNA-PGK-puroR
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGL3-ERCC6-hCtRNA-Nicking/PegRNA-PGK-puroR载体质粒基本信息
pGL3-ERCC6-hCtRNA-Nicking/PegRNA-PGK-puroR质粒图谱载体图谱和pGL3-ERCC6-hCtRNA-Nicking/PegRNA-PGK-puroR载体序列质粒序列多克隆位点信息
pGL3-ERCC6-hCtRNA-Nicking/PegRNA-PGK-puroR质粒载体简介
pGL3-ERCC6-hCtRNA-Nicking/PegRNA-PGK-puroR质粒序列载体序列
LOCUS pGL3-ERCC6-hCtRNA-Nicking/PegRNA-PGK-puroR 4950 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4950)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4950
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 21..55
/label=OL2640
primer_bind 22..61
/label=OL2721
primer_bind 24..40
/label=OL1149
primer_bind 24..40
/label=OL2861
misc_feature 41..115
/label=hCtRNA
primer_bind 56..79
/label=OL464
primer_bind complement(98..115)
/label=OL465
primer_bind complement(101..135)
/label=OL2651
primer_bind complement(101..115)
/label=OL2653
primer_bind 116..164
/label=OL2726
primer_bind 116..151
/label=OL2652
misc_feature 116..135
/label=ERCC6 nickingRNA
misc_RNA 136..211
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 136..176
/label=OL462
primer_bind complement(194..230)
/label=OL463
misc_feature 212..286
/label=hCtRNA
primer_bind 212..232
/label=OL2721
primer_bind 212..226
/label=OL2640
primer_bind 227..250
/label=OL464
primer_bind complement(269..287)
/label=OL465
primer_bind complement(272..306)
/label=OL2653
primer_bind complement(272..286)
/label=OL2651
primer_bind 287..310
/label=OL2654
misc_feature 287..306
/label=ERCC6 spacer
primer_bind 287..301
/label=OL2655
misc_RNA 307..382
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 307..347
/label=OL462
primer_bind 307..335
/label=OL2726
primer_bind 307..322
/label=OL2652
primer_bind complement(365..383)
/label=OL463
misc_feature 383..397
/label=RTT
primer_bind complement(393..439)
/label=OL2727
misc_feature 397
/label=a to g
misc_feature 398..415
/label=PBS
primer_bind complement(398..414)
/label=OL2654
primer_bind complement(400..439)
/label=OL2655
primer_bind complement(408..427)
/label=OL2756
primer_bind 421..440
/label=OL458
misc_feature 459..576
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 625..1135
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1054..1072
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 1157..1756
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1157..1176)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1653..1673
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 1903..2024
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1914..1933)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 1968..1987
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2272..2289)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2443..3031)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2523..2542)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3202..4062)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3825..3844
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4063..4167)
/gene="bla"
/label=AmpR promoter
rep_origin 4194..4649
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4281..4300)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4491..4512
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 4780..4828
/note="synthetic polyadenylation signal"
misc_feature 4842..4933
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 4882..4901
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 agtgaacgga tctcgacggt atcgatcacg agactagcct agagggggta tagctcagtg
61 gtagagcatt tgactgcaga tcaagaggtc cccggttcaa atccgggtgc cccctgtcag
121 tgattagtgg aaatggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
181 tcaacttgaa aaagtggcac cgagtcggtg cagagggggt atagctcagt ggtagagcat
241 ttgactgcag atcaagaggt ccccggttca aatccgggtg cccccttcaa tgctttcatc
301 accagagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
361 aaaagtggca ccgagtcggt gcacttctat gccatccggt gatgaaagca ttgatttttt
421 ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
481 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
541 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
601 gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag gcagccctgg
661 gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc
721 tgggactcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct
781 tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt
841 cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg
901 acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg
961 ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg
1021 ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga
1081 gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga
1141 tccaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
1201 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
1261 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
1321 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
1381 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
1441 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
1501 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
1561 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
1621 ggtgcccgcc ttcctggaaa cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
1681 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
1741 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
1801 accccatgca tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
1861 ctttctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt
1921 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
1981 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
2041 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
2101 tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag agccttcaac
2161 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
2221 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
2281 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
2341 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
2401 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
2461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
2521 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
2581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
2641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
2701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
2761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
2821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
2881 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
2941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
3001 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
3061 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
3121 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
3181 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
3241 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
3301 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc
3361 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
3421 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
3481 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
3541 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
3601 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
3661 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
3721 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
3781 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
3841 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
3901 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
3961 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
4021 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
4081 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
4141 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
4201 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
4261 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
4321 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
4381 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
4441 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
4501 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
4561 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
4621 ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc gcaactgttg
4681 ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt
4741 aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc tttattttca
4801 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
4861 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
4921 agaacatttc tctatcgata ggtaccgatt
//
pGL3-ERCC6-hCtRNA-Nicking/PegRNA-PGK-puroR载体图谱质粒图谱pdf版和pGL3-ERCC6-hCtRNA-Nicking/PegRNA-PGK-puroR载体序列质粒序列等相关资料下载
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