pEF1a-RT-MLH1dn-EGFP

LOCUS       pEF1a-RT-MLH1dn-EGFP            9267 bp DNA     circular SYN 07-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     80..122
                     /label=OL2675
     enhancer        87..105
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        106..1284
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     primer_bind     106..122
                     /label=OL2712
     intron          337..1275
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     359..376
                     /label=ef1a-seq1
     primer_bind     1135..1154
                     /label=ef1a-seq2
     primer_bind     1232..1252
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(1256..1309)
                     
     primer_bind     complement(1285..1313)
                     /label=OL2625
     primer_bind     1291..1310
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1291..1309
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1291..1309
                     /label=OL1124
     primer_bind     complement(1306..1325)
                     /label=OL1206
     primer_bind     complement(1344..1358)
                     /label=OL2538
     primer_bind     1350..1407
                     /label=OL1012
     primer_bind     complement(1353..1409)
                     /label=OL1013
     primer_bind     complement(1357..1379)
                     /label=OL1036
     CDS             1359..1379
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     1360..1405
                     /label=OL1037
     primer_bind     complement(1360..1379)
                     /label=OL1298
     CDS             1379
                     /label=SGGSx2-sv40NLS-SGGSx2
                     /note="/created_by=chenp"
     CDS             1380..3410
                     /label=RT codon optimized Genscript
                     /note="/created_by=jessiedavis"
     primer_bind     complement(1992..2031)
                     /label=OL1894
     primer_bind     2074..2088
                     /label=OL1416
     primer_bind     complement(2234..2252)
                     /label=OL1920
     primer_bind     complement(2263..2294)
                     /label=OL2389
     primer_bind     3109..3123
                     /label=OL1417
     primer_bind     3344..3366
                     /label=OL447
     primer_bind     3411..3432
                     /label=OL2671
     CDS             3456..3476
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3486..3512
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     primer_bind     complement(3490..3512)
                     /label=OL2672
     CDS             3522..3578
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     3522..3578
                     /label=OL866
     primer_bind     complement(3522..3578)
                     /label=OL867
     primer_bind     3522..3548
                     /label=OL2251
     primer_bind     3522..3546
                     /label=OL899
     primer_bind     3522..3545
                     /label=PE001
     primer_bind     3546..3579
                     /label=OL1687
     primer_bind     complement(3559..3579)
                     /label=OL308
     primer_bind     complement(3560..3579)
                     /label=PE002
     primer_bind     3560..3579
                     /label=OL309
     CDS             3579..5834
                     /label=MLH1 del754-756 genscript codon
                     /note="/created_by=chenp"
                     /note="/modified_by=chenp"
     primer_bind     3633..3668
                     /label=OL532
     primer_bind     complement(3939..3977)
                     /label=OL531
     primer_bind     4032..4046
                     /label=OL1418
     CDS             4971..5003
                     /codon_start=1
                     /label=MLH1 NLS
                     /translation="SSNPRKRHRED"
     primer_bind     5595..5614
                     /label=OL1414
     primer_bind     complement(5595..5614)
                     /label=OL1415
     primer_bind     5805..5859
                     /label=OL899
     primer_bind     5834..5858
                     /label=PE001
     CDS             5835..5891
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     5835..5891
                     /label=OL866
     primer_bind     complement(5835..5891)
                     /label=OL867
     primer_bind     5835..5861
                     /label=OL2251
     primer_bind     5859..5912
                     /label=OL1687
     primer_bind     complement(5872..5910)
                     /label=OL308
     primer_bind     5873..5910
                     /label=OL309
     primer_bind     complement(5873..5899)
                     /label=PE002
     primer_bind     5883..5909
                     /label=PE003
     CDS             5892..6611
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     5892..5912
                     /label=OL1686
     primer_bind     5892..5912
                     /label=OL1704
     primer_bind     complement(6113..6132)
                     /label=OL957
     primer_bind     6545..6565
                     /label=OL697
     primer_bind     6555..6569
                     /label=OL1937
     primer_bind     complement(6586..6611)
                     /label=OL174
     primer_bind     complement(6587..6641)
                     /label=OL900
     primer_bind     complement(6588..6612)
                     /label=PE004
     primer_bind     complement(6588..6611)
                     /label=OL2710
     primer_bind     complement(6588..6608)
                     /label=OL1833
     primer_bind     complement(6592..6631)
                     /label=OL1205
     primer_bind     complement(6592..6631)
                     /label=OL1215
     primer_bind     complement(6593..6634)
                     /label=OL1146
     primer_bind     complement(6594..6636)
                     /label=OL1038
     primer_bind     6608..6641
                     /label=OL1014
     primer_bind     6612..6631
                     /label=OL1207
     primer_bind     6612..6626
                     /label=OL2537
     primer_bind     6617..6640
                     /label=OL1035
     CDS             6617..6634
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     6617..6634
                     /label=OL1133
     primer_bind     complement(6617..6634)
                     /label=OL1152
     primer_bind     complement(6657..6674)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6663..6887
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal
                     牛生长激素聚腺苷酸化信号
                     牛生长激素（BGH）多聚腺苷酸化信号能够保证高效的转录终止和mRNA的poly A结构"
     primer_bind     6663..6682
                     /label=OL1407
     primer_bind     complement(6668..6687)
                     /label=OL1410
     primer_bind     complement(6707..6724)
                     /label=OL698
     primer_bind     complement(6709..6728)
                     /label=OL1938
     primer_bind     complement(6868..6887)
                     /label=OL1684
     primer_bind     complement(6958..6974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6958..6974)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6971..6993)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6982..6998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7006..7036)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7051..7072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7189..7206)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7360..7948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7440..7459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7542..7561)
                     /label=OL1041
     primer_bind     complement(7675..7694)
                     /label=OL631
     primer_bind     7731..7750
                     /label=OL798
     primer_bind     7992..8011
                     /label=OL659
     CDS             complement(8119..8979)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8576..8595
                     /label=OL1922
     primer_bind     8742..8761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(8742..8761)
                     /label=OL1923
     primer_bind     8895..8914
                     /label=OL1033
     primer_bind     complement(8895..8914)
                     /label=OL1034
     promoter        complement(8980..9084)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9163..9182)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9163..9182
                     /label=OL1919
ORIGIN
        1 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
       61 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagggctc cggtgcccgt
      121 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      181 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      241 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      301 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      361 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      421 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      481 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      541 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      601 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      661 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      721 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      781 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      841 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      901 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
      961 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1021 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1081 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1141 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1201 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1261 tttcttccat ttcaggtgtc gtgaggccgc taatacgact cactataggg agagccgcca
     1321 ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc aaagaagaag cggaaagtca
     1381 ccctgaacat tgaagacgag tatagactgc atgaaacaag caaggaaccc gacgtgtccc
     1441 tgggctccac ctggctgtcc gactttcccc aggcctgggc cgagacagga ggaatgggcc
     1501 tggccgtgcg gcaggcaccc ctgatcatcc ctctgaaggc cacctctaca cccgtgagca
     1561 tcaagcagta ccctatgtct caggaggcca gactgggcat caagcctcac atccagaggc
     1621 tgctggacca gggcatcctg gtgccatgcc agagcccctg gaacacacca ctgctgcccg
     1681 tgaagaagcc aggcaccaat gactatagac ccgtgcagga tctgagagag gtgaacaaga
     1741 gggtggagga tatccacccc accgtgccca acccttacaa tctgctgtcc ggcctgcccc
     1801 cttctcacca gtggtataca gtgctggacc tgaaggatgc cttcttttgt ctgagactgc
     1861 accctaccag ccagccactg ttcgcctttg agtggaggga ccctgagatg ggcatctctg
     1921 gccagctgac ctggacacgc ctgcctcagg gcttcaagaa tagcccaaca ctgtttaacg
     1981 aggccctgca ccgcgacctg gcagatttcc ggatccagca cccagatctg atcctgctgc
     2041 agtacgtgga cgatctgctg ctggccgcca ccagcgagct ggattgccag cagggaacac
     2101 gcgccctgct gcagaccctg ggaaacctgg gatatagggc atccgccaag aaggcccaga
     2161 tctgtcagaa gcaggtgaag tacctgggct atctgctgaa ggagggccag agatggctga
     2221 cagaggccag gaaggagaca gtgatgggcc agccaacacc caagacccca agacagctga
     2281 gggagttcct gggcaaagca ggattttgca ggctgttcat cccaggattc gcagagatgg
     2341 cagcacctct gtacccactg accaagccgg gcaccctgtt taattggggc cctgaccagc
     2401 agaaggccta tcaggagatc aagcaggccc tgctgacagc accagccctg ggcctgccag
     2461 acctgaccaa gcctttcgag ctgtttgtgg atgagaagca gggctacgcc aagggcgtgc
     2521 tgacccagaa gctgggacca tggagacggc ccgtggccta tctgtccaag aagctggacc
     2581 cagtggcagc aggatggcca ccatgcctga ggatggtggc agcaatcgcc gtgctgacaa
     2641 aggatgccgg caagctgacc atgggacagc cactggtcat cctggcacca cacgcagtgg
     2701 aggccctggt gaagcagcct ccagatcgct ggctgtctaa cgcccggatg acacactacc
     2761 aggccctgct gctggacacc gatcgcgtgc agtttggccc tgtggtggcc ctgaatccag
     2821 ccaccctgct gcctctgcca gaggagggcc tgcagcacaa ctgtctggac atcctggcag
     2881 aggcacacgg aacaaggcca gacctgaccg atcagcccct gcctgacgcc gatcacacat
     2941 ggtataccga tggaagctcc ctgctgcagg agggccagag gaaggcagga gcagcagtga
     3001 ccacagagac agaagtgatc tgggccaagg ccctgccagc aggcacatcc gcccagcggg
     3061 ccgagctgat cgccctgacc caggccctga agatggccga gggcaagaag ctgaacgtgt
     3121 acacagactc cagatatgcc ttcgccaccg cacacatcca cggagagatc tacaggcgcc
     3181 ggggctggct gacctctgag ggcaaggaga tcaagaacaa ggatgagatc ctggccctgc
     3241 tgaaggccct gtttctgccc aagcggctga gcatcatcca ctgtcctgga caccagaagg
     3301 gacactccgc cgaggcaagg ggcaatcgga tggccgacca ggccgccaga aaggctgcta
     3361 ttactgaaac tcccgacact tccactctgc tgattgaaaa ctcctcccct tctggcggct
     3421 caaaaagaac cgccgacggc agcgaattcg agtctcccaa gaagaagagg aaagtcggct
     3481 ctggccctgc cgctaagaga gtgaagctgg acggaagcgg agctactaac ttcagcctgc
     3541 tgaagcaggc tggagacgtg gaggagaacc ctggacctag cttcgttgct ggagtcatcc
     3601 ggagactgga cgagacagtg gtgaacagaa ttgccgccgg cgaggtgatc cagagacctg
     3661 ccaatgcaat aaaggagatg atcgagaact gtctggacgc caagtccaca agcattcagg
     3721 tgatcgtgaa ggagggcgga ctgaagctga tccagatcca agacaacggc acaggcatca
     3781 gaaaggaaga tctggacatc gtgtgtgaac ggttcaccac atctaagctg cagtcttttg
     3841 aggatctggc ctctatcagt acctacggct tcagaggcga ggccctggcc agcatcagcc
     3901 acgtggccca tgtgaccatc accaccaaaa ccgccgacgg caaatgcgct tatcgcgcta
     3961 gctacagcga cggcaagctg aaagccccgc caaagccttg cgccggcaac cagggtacac
     4021 agataacagt ggaggatctg ttctacaaca tcgccacccg gagaaaggcc ctgaaaaatc
     4081 ccagcgagga gtacggcaag atcctggaag tcgtcggcag atactccgtg cacaacgccg
     4141 gaatcagctt tagcgtaaag aagcagggag aaaccgtggc cgatgtgcgc accctgccaa
     4201 atgccagcac cgtggataac atcagaagca ttttcggaaa tgccgtgtcc agagaactga
     4261 tcgagatcgg ctgcgaagat aagaccctgg cttttaagat gaacggctac atcagcaacg
     4321 ccaattactc tgtgaagaag tgcatctttc ttctgttcat caaccacaga ctggtggaaa
     4381 gcaccagcct gcggaaagcc atcgagacag tgtacgccgc ctacctgcct aagaacaccc
     4441 accccttcct gtacctgagc ctcgagatca gccctcagaa cgtggacgtc aatgtgcatc
     4501 ctacaaagca cgaggtgcac ttcctgcacg aggaaagcat cctggaaaga gtgcagcagc
     4561 acattgagag caagctgctg ggctctaaca gcagcagaat gtacttcaca cagaccctgc
     4621 tgcctggcct ggccggcccc tcaggcgaaa tggttaagtc cacaacctct ctgacctcat
     4681 ccagcaccag cggttcttcc gataaggtgt acgcccacca gatggtgcgg accgactctc
     4741 gggagcagaa gctggacgcc tttctgcaac ctctgagcaa acctctgagc tctcagcctc
     4801 aggccatcgt gaccgaggac aagacagata tctcctccgg ccgtgccaga cagcaggacg
     4861 aagaaatgct cgagctgcca gctcctgccg aggtggccgc caagaaccag agcctggagg
     4921 gagataccac aaagggcacc agcgaaatga gcgagaagcg gggccctacc tccagcaacc
     4981 ccagaaaacg gcaccgggag gacagcgacg tggaaatggt ggaggacgac agcagaaagg
     5041 aaatgacagc cgcttgtacc cctagaagaa gaatcatcaa cctgacctcc gtgctgagcc
     5101 tgcaggagga gatcaacgag cagggccacg aggtgctgag agagatgctg cacaatcaca
     5161 gcttcgtggg ctgcgtgaac cctcaatggg ccctggctca gcatcaaaca aagctgtacc
     5221 tgctgaacac caccaagctg agcgaagagc tgttctacca gatcctcatc tacgacttcg
     5281 ccaacttcgg cgtgctacgc ctgagcgagc ccgcccctct gtttgacctg gccatgctgg
     5341 ctctggatag cccagaaagc ggctggacag aagaggacgg acctaaagag gggctggctg
     5401 aatacatcgt ggagttcctg aagaaaaagg ccgagatgct ggccgactac ttttctctgg
     5461 aaatcgacga ggaaggcaac ctgatcggcc tgcctctgct gatcgataac tacgtgcctc
     5521 ccctggaagg cctgcccatc ttcatcctga gactggctac agaggtgaac tgggacgagg
     5581 aaaaggaatg cttcgagtct ctgagcaagg agtgcgccat gttctatagc atcagaaaac
     5641 agtacatctc tgaagagagc actctgtctg gccagcagag tgaagtgccc ggaagcatcc
     5701 ccaacagctg gaagtggacc gtggaacaca tcgtgtacaa ggccctgcgg agccacattc
     5761 tccctcctaa gcacttcacc gaggacggca acatcctgca gctggccaac ctgcccgacc
     5821 tttataaggt tttcgctact aacttcagcc tgctgaagca ggctggagac gtggaggaga
     5881 accctggacc tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     5941 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     6001 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     6061 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     6121 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     6181 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     6241 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     6301 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     6361 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     6421 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     6481 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     6541 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6601 tgtacaagta accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     6661 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6721 cctggaaggt gccactccca ctgtcctttc ctaataaaat gagaaaattg catcgcattg
     6781 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6841 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6901 aagaaccagc tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc
     6961 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7021 aagcataaag tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt
     7081 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7141 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7201 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7261 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7321 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7381 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7441 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7501 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7561 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7621 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7681 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7741 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7801 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7861 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7921 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7981 cactcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8041 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8101 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8161 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8221 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8281 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8341 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8401 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8461 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8521 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8581 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8641 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8701 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8761 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8821 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8881 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8941 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9001 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9061 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     9121 atcgggagat cgatctcccg atcccctagg gtctactctc agtacaatct gctctgatgc
     9181 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     9241 gagcaaaatt taagctacaa caaggca
//