pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NCOA1-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NCOA1-FLAG              6383 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3096
                     /codon_start=1
                     /gene="NCOA1"
                     /gene_synonym="bHLHe42; bHLHe74; F-SRC-1; KAT13A; 
                     RIP160;SRC1"
                     /product="nuclear receptor coactivator 1 isoform 1"
                     /EC_number="2.3.1.48"
                     /label=NCOA1
                     /note="isoform 1 is encoded by transcript variant 1;steroid
                     receptor coactivator-1; Hin-2 protein; renal carcinoma 
                     antigen NY-REN-52; class E basic helix-loop-helix protein 
                     74"
                     /db_xref="CCDS:CCDS1712.1"
                     /db_xref="GeneID:8648"
                     /db_xref="HGNC:HGNC:7668"
                     /db_xref="MIM:602691"
                     /protein_id="NP_003734.3"
                     /translation="RNDEKALLEQLVSFLSGKDETELAELDRALGIDKLVQGGGLDVLS
                     ERFPPQQATPPLIMEERPNLYSQPYSSPSPTANLPSPFQGMVRQKPSLGTMPVQVTPPR
                     GAFSPGMGMQPRQTLNRPPAAPNQLRLQLQQRLQGQQQLIHQNRQAILNQFAATAPVGI
                     NMRSGMQQQITPQPPLNAQMLAQRQRELYSQQHRQRQLIQQQRAMLMRQQSFGNNLPPS
                     SGLPVQMGNPRLPQGAPQQFPYPPNYGTNPGTPPASTSPFSQLAANPEASLANRNSMVS
                     RGMTGNIGGQFGTGINPQMQQNVFQYPGAGMVPQGEANFAPSLSPGSSMVPMPIPPPQS
                     SLLQQTPPASGYQSPDMKAWQQGAIGNNNVFSQAVQNQPTPAQPGVYNNMSITVSMAGG
                     NTNVQNMNPMMAQMQMSSLQMPGMNTVCPEQINDPALRHTGLYCNQLSSTDLLKTEADG
                     TQQVQQVQVFADVQCTVNLVGGDPYLNQPGPLGTQKPTSGPQTPQAQQKSLLQQLLTE"
     misc_feature    2412..2417
                     /label=SDS
     misc_feature    2554..2559
                     /label=SDS
     misc_feature    2557..2562
                     /label=SDS
     misc_feature    3072..3077
                     /label=SDS
     CDS             3103..3168
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3172..3603
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3316..3321
                     /label=SDS
     repeat_region   3646..3786
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3646..3775
                     /label=AAV2 ITR
     repeat_region   3647..3787
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3861..4316
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4598..4702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4703..5563
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5734..6322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggag aaatgatgag
     1561 aaggctcttc ttgaacagct ggtatccttc cttagtggca aagatgaaac tgagctagct
     1621 gaactagaca gagctctggg aattgacaaa cttgttcagg ggggtggatt agatgtatta
     1681 tcagagagat ttccaccaca acaagcaacg ccacctttga tcatggaaga aagacccaac
     1741 ctttattccc agccttactc ttctccttct cctactgcca atctccctag ccctttccaa
     1801 ggcatggtca ggcaaaaacc ttcactgggg acgatgcctg ttcaagtaac acctccccga
     1861 ggtgcttttt cacctggcat gggcatgcag cccaggcaaa ctctaaacag acctccggct
     1921 gcacctaacc agcttcgact tcaactacag cagcgattac agggacaaca gcagttgata
     1981 caccaaaatc ggcaagctat cttaaaccag tttgcagcaa ctgctcctgt tggcatcaat
     2041 atgagatcag gcatgcaaca gcaaattaca cctcagccac ccctgaatgc tcaaatgttg
     2101 gcacaacgtc agcgggaact gtacagtcaa cagcaccgac agaggcagct aatacagcag
     2161 caaagagcca tgcttatgag gcagcaaagc tttgggaaca acctccctcc ctcatctgga
     2221 ctaccagttc aaatggggaa cccccgtctt cctcagggtg ctccacagca attcccctat
     2281 ccaccaaact atggtacaaa tccaggaacc ccacctgctt ctaccagccc gttttcacaa
     2341 ctagcagcaa atcctgaagc atccttggcc aaccgcaaca gcatggtgag cagaggcatg
     2401 acaggaaaca taggaggaca gtttggcact ggaatcaatc ctcagatgca gcagaatgtc
     2461 ttccagtatc caggagcagg aatggttccc caaggtgagg ccaactttgc tccatctcta
     2521 agccctggga gctccatggt gccgatgcca atccctcctc ctcagagttc tctgctccag
     2581 caaactccac ctgcctccgg gtatcagtca ccagacatga aggcctggca gcaaggagcg
     2641 ataggaaaca acaatgtgtt cagtcaagct gtccagaacc agcccacgcc tgcacagcca
     2701 ggagtataca acaacatgag catcaccgtt tccatggcag gtggaaatac gaatgttcag
     2761 aacatgaacc caatgatggc ccagatgcag atgagctctt tgcagatgcc aggaatgaac
     2821 actgtgtgcc ctgagcagat aaatgatccc gcactgagac acacaggcct ctactgcaac
     2881 cagctctcat ccactgacct tctcaaaaca gaagcagatg gaacccagca ggtgcaacag
     2941 gttcaggtgt ttgctgacgt ccagtgtaca gtgaatctgg taggcgggga cccttacctg
     3001 aaccagcctg gtccactggg aactcaaaag cccacgtcag gaccacagac cccccaggcc
     3061 cagcagaaga gcctccttca gcagctactg actgaatgta cagactacaa agaccatgac
     3121 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta actcgagata
     3181 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3241 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3301 tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca
     3361 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     3421 tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc tagctttatt
     3481 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3541 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
     3601 taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
     3661 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     3721 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     3781 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3841 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3901 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     3961 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4021 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     4081 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4141 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     4201 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     4261 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     4321 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     4381 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     4441 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     4501 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     4561 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4621 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4681 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4741 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4801 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4861 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4921 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4981 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5041 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5101 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5161 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5221 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5281 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5341 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5401 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5461 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5521 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5581 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5641 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5701 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5761 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5821 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5881 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5941 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6001 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6061 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6121 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6181 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6241 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6301 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6361 ttgctggcct tttgctcaca tgt
//