| 质粒名称: | pAAVLINK-3'-CREBBP-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001722 |
| 产品用途: | 3' AAVLINK plasmid for CREBBP expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CREBBP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-CREBBP-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-CREBBP-FLAG载体质粒基本信息
pAAVLINK-3'-CREBBP-FLAG质粒图谱载体图谱和pAAVLINK-3'-CREBBP-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-CREBBP-FLAG质粒载体简介
pAAVLINK-3'-CREBBP-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-CREBBP-FLAG 7122 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7122)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7122
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3861
/codon_start=1
/label=CREBBP
/translation="GENVTLGDDPSQPQTTISKDQFEKKKNDTLDPEPFVDCKECGRKM
HQICVLHYDIIWPSGFVCDNCLKKTGRPRKENKFSAKRLQTTRLGNHLEDRVNKFLRRQ
NHPEAGEVFVRVVASSDKTVEVKPGMKSRFVDSGEMSESFPYRTKALFAFEEIDGVDVC
FFGMHVQEYGSDCPPPNTRRVYISYLDSIHFFRPRCLRTAVYHEILIGYLEYVKKLGYV
TGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAFAERIIHDYKDIFKQA
TEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKKEESTAASETTEGSQGDSKNA
KKKNNKKTNKNKSSISRANKKKPSMPNVSNDLSQKLYATMEKHKEVFFVIHLHAGPVIN
TLPPIVDPDPLLSCDLMDGRDAFLTLARDKHWEFSSLRRSKWSTLCMLVELHTQGQDRF
VYTCNECKHHVETRWHCTVCEDYDLCINCYNTKSHAHKMVKWGLGLDDEGSSQGEPQSK
SPQESRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPVCKQ
LIALCCYHAKHCQENKCPVPFCLNIKHKLRQQQIQHRLQQAQLMRRRMATMNTRNVPQQ
SLPSPTSAPPGTPTQQPSTPQTPQPPAQPQPSPVSMSPAGFPSVARTQPPTTVSTGKPT
SQVPAPPPPAQPPPAAVEAARQIEREAQQQQHLYRVNINNSMPPGRTGMGTPGSQMAPV
SLNVPRPNQVSGPVMPSMPPGQWQQAPLPQQQPMPGLPRPVISMQAQAAVAGPRMPSVQ
PPRSISPSALQDLLRTLKSPSSPQQQQQVLNILKSNPQLMAAFIKQRTAKYVANQPGMQ
PQPGLQSQPGMQPQPGMHQQPSLQNLNAMQAGVPRPGVPPQQQAMGGLNPQGQALNIMN
PGHNPNMASMNPQYREMLRRQLLQQQQQQQQQQQQQQQQQQGSAGMAGGMAGHGQFQQP
QGPGGYPPAMQQQQRMQQHLPLQGSSMGQMAAQMGQLGQMGQPGLGADSTPNIQQALQQ
RILQQQQMKQQIGSPGQPNPMSPQQHMLSGQPQASHLPGQQIATSLSNQVRSPAPVQSP
RPQSQPPHSSPSPRIQPQPSPHHVSPQTGSPHPGLAVTMASSIDQGHLGNPEQSAMLPQ
LNTPSRSALSSELSLVGDTTGDTLEKFVEGL"
regulatory 1402..1411
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 1421..1426
/label=SDS
misc_feature 1571..1576
/label=SDS
misc_feature 2389..2394
/label=SDS
CDS 3868..3933
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3937..4368
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4081..4086
/label=SDS
repeat_region 4385..4525
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4385..4514
/label=AAV2 ITR
rep_origin 4600..5055
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5337..5441
/gene="bla"
/label=AmpR promoter
CDS 5442..6302
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6473..7061
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag ggcgagaatg tgaccctggg tgacgaccct
301 tcacagcccc agacgacaat ttcaaaggat cagtttgaaa agaagaaaaa tgatacctta
361 gaccccgaac ctttcgttga ttgcaaggag tgtggccgga agatgcatca gatttgcgtt
421 ctgcactatg acatcatttg gccttcaggt tttgtgtgcg acaactgctt gaagaaaact
481 ggcagacctc gaaaagaaaa caaattcagt gctaagaggc tgcagaccac aagactggga
541 aaccacttgg aagaccgagt gaacaaattt ttgcggcgcc agaatcaccc tgaagccggg
601 gaggtttttg tccgagtggt ggccagctca gacaagacgg tggaggtcaa gcccgggatg
661 aagtcacggt ttgtggattc tggggaaatg tctgaatctt tcccatatcg aaccaaagct
721 ctgtttgctt ttgaggaaat tgacggcgtg gatgtctgct tttttggaat gcacgtccaa
781 gaatacggct ctgattgccc ccctccaaac acgaggcgtg tgtacatttc ttatctggat
841 agtattcatt tcttccggcc acgttgcctc cgcacagccg tttaccatga gatccttatt
901 ggatatttag agtatgtgaa gaaattaggg tatgtgacag ggcacatctg ggcctgtcct
961 ccaagtgaag gagatgatta catcttccat tgccacccac ctgatcaaaa aatacccaag
1021 ccaaaacgac tgcaggagtg gtacaaaaag atgctggaca aggcgtttgc agagcggatc
1081 atccatgact acaaggatat tttcaaacaa gcaactgaag acaggctcac cagtgccaag
1141 gaactgccct attttgaagg tgatttctgg cccaatgtgt tagaagagag cattaaggaa
1201 ctagaacaag aagaagagga gaggaaaaag gaagagagca ctgcagccag tgaaaccact
1261 gagggcagtc agggcgacag caagaatgcc aagaagaaga acaacaagaa aaccaacaag
1321 aacaaaagca gcatcagccg cgccaacaag aagaagccca gcatgcccaa cgtgtccaat
1381 gacctgtccc agaagctgta tgccaccatg gagaagcaca aggaggtctt cttcgtgatc
1441 cacctgcacg ctgggcctgt catcaacacc ctgcccccca tcgtcgaccc cgaccccctg
1501 ctcagctgtg acctcatgga tgggcgcgac gccttcctca ccctcgccag agacaagcac
1561 tgggagttct cctccttgcg ccgctccaag tggtccacgc tctgcatgct ggtggagctg
1621 cacacccagg gccaggaccg ctttgtctac acctgcaacg agtgcaagca ccacgtggag
1681 acgcgctggc actgcactgt gtgcgaggac tacgacctct gcatcaactg ctataacacg
1741 aagagccatg cccataagat ggtgaagtgg gggctgggcc tggatgacga gggcagcagc
1801 cagggcgagc cacagtcaaa gagcccccag gagtcacgcc ggctgagcat ccagcgctgc
1861 atccagtcgc tggtgcacgc gtgccagtgc cgcaacgcca actgctcgct gccatcctgc
1921 cagaagatga agcgggtggt gcagcacacc aagggctgca aacgcaagac caacgggggc
1981 tgcccggtgt gcaagcagct catcgccctc tgctgctacc acgccaagca ctgccaagaa
2041 aacaaatgcc ccgtgccctt ctgcctcaac atcaaacaca agctccgcca gcagcagatc
2101 cagcaccgcc tgcagcaggc ccagctcatg cgccggcgga tggccaccat gaacacccgc
2161 aacgtgcctc agcagagtct gccttctcct acctcagcac cgcccgggac ccccacacag
2221 cagcccagca caccccagac gccgcagccc cctgcccagc cccaaccctc acccgtgagc
2281 atgtcaccag ctggcttccc cagcgtggcc cggactcagc cccccaccac ggtgtccaca
2341 gggaagccta ccagccaggt gccggccccc ccacccccgg cccagccccc tcctgcagcg
2401 gtggaagcgg ctcggcagat cgagcgtgag gcccagcagc agcagcacct gtaccgggtg
2461 aacatcaaca acagcatgcc cccaggacgc acgggcatgg ggaccccggg gagccagatg
2521 gcccccgtga gcctgaatgt gccccgaccc aaccaggtga gcgggcccgt catgcccagc
2581 atgcctcccg ggcagtggca gcaggcgccc cttccccagc agcagcccat gccaggcttg
2641 cccaggcctg tgatatccat gcaggcccag gcggccgtgg ctgggccccg gatgcccagc
2701 gtgcagccac ccaggagcat ctcacccagc gctctgcaag acctgctgcg gaccctgaag
2761 tcgcccagct cccctcagca gcaacagcag gtgctgaaca ttctcaaatc aaacccgcag
2821 ctaatggcag ctttcatcaa acagcgcaca gccaagtacg tggccaatca gcccggcatg
2881 cagccccagc ctggcctcca gtcccagccc ggcatgcaac cccagcctgg catgcaccag
2941 cagcccagcc tgcagaacct gaatgccatg caggctggcg tgccgcggcc cggtgtgcct
3001 ccacagcagc aggcgatggg aggcctgaac ccccagggcc aggccttgaa catcatgaac
3061 ccaggacaca accccaacat ggcgagtatg aatccacagt accgagaaat gttacggagg
3121 cagctgctgc agcagcagca gcaacagcag cagcaacaac agcagcaaca gcagcagcag
3181 caagggagtg ccggcatggc tgggggcatg gcggggcacg gccagttcca gcagcctcaa
3241 ggacccggag gctacccacc ggccatgcag cagcagcagc gcatgcagca gcatctcccc
3301 ctccagggca gctccatggg ccagatggcg gctcagatgg gacagcttgg ccagatgggg
3361 cagccggggc tgggggcaga cagcaccccc aacatccagc aagccctgca gcagcggatt
3421 ctgcagcaac agcagatgaa gcagcagatt gggtccccag gccagccgaa ccccatgagc
3481 ccccagcaac acatgctctc aggacagcca caggcctcgc atctccctgg ccagcagatc
3541 gccacgtccc ttagtaacca ggtgcggtct ccagcccctg tccagtctcc acggccccag
3601 tcccagcctc cacattccag cccgtcacca cggatacagc cccagccttc gccacaccac
3661 gtctcacccc agactggttc cccccacccc ggactcgcag tcaccatggc cagctccata
3721 gatcagggac acttggggaa ccccgaacag agtgcaatgc tcccccagct gaacaccccc
3781 agcaggagtg cgctgtccag cgaactgtcc ctggtcgggg acaccacggg ggacacgcta
3841 gagaagtttg tggagggctt gtgtacagac tacaaagacc atgacggtga ttataaagat
3901 catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt
3961 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
4021 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
4081 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
4141 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg
4201 aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat
4261 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
4321 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaacg gaccgagcgg
4381 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
4441 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
4501 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
4561 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
4621 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
4681 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
4741 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
4801 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
4861 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
4921 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
4981 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
5041 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
5101 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
5161 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
5221 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
5281 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
5341 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
5401 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
5461 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
5521 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
5581 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
5641 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
5701 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
5761 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
5821 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
5881 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
5941 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
6001 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
6061 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
6121 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
6181 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
6241 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
6301 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
6361 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
6421 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
6481 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
6541 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6601 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
6661 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
6721 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
6781 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
6841 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
6901 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
6961 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
7021 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
7081 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
