pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN1A-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SCN1A-FLAG               7083 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3802
                     /codon_start=1
                     /label=3' SCN1A
                     /translation="VFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTAN
                     ALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIF
                     SIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGF
                     GYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFI
                     GVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFV
                     TRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRH
                     YYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGI
                     RTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMI
                     CLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISF
                     LVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAA
                     ALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEER
                     FMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGA
                     NLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK"
     misc_feature    2797..2802
                     /label=SDS
     CDS             3803..3868
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3872..4303
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4016..4021
                     /label=SDS
     repeat_region   4346..4486
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4346..4475
                     /label=AAV2 ITR
     repeat_region   4347..4487
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4561..5016
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5298..5402
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5403..6263
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6434..7022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggttt tcacttacat
     1561 tttcattctg gaaatgcttc taaaatgggt ggcatatggc tatcaaacat atttcaccaa
     1621 tgcctggtgt tggctggact tcttaattgt tgatgtttca ttggtcagtt taacagcaaa
     1681 tgccttgggt tactcagaac ttggagccat caaatctctc aggacactaa gagctctgag
     1741 acctctaaga gccttatctc gatttgaagg gatgagggtg gttgtgaatg cccttttagg
     1801 agcaattcca tccatcatga atgtgcttct ggtttgtctt atattctggc taattttcag
     1861 catcatgggc gtaaatttgt ttgctggcaa attctaccac tgtattaaca ccacaactgg
     1921 tgacaggttt gacatcgaag acgtgaataa tcatactgat tgcctaaaac taatagaaag
     1981 aaatgagact gctcgatgga aaaatgtgaa agtaaacttt gataatgtag gatttgggta
     2041 tctctctttg cttcaagttg ccacattcaa aggatggatg gatataatgt atgcagcagt
     2101 tgattccaga aatgtggaac tccagcctaa gtatgaagaa agtctgtaca tgtatcttta
     2161 ctttgttatt ttcatcatct ttgggtcctt cttcaccttg aacctgttta ttggtgtcat
     2221 catagataat ttcaaccagc agaaaaagaa gtttggaggt caagacatct ttatgacaga
     2281 agaacagaag aaatactata atgcaatgaa aaaattagga tcgaaaaaac cgcaaaagcc
     2341 tatacctcga ccaggaaaca aatttcaagg aatggtcttt gacttcgtaa ccagacaagt
     2401 ttttgacata agcatcatga ttctcatctg tcttaacatg gtcacaatga tggtggaaac
     2461 agatgaccag agtgaatatg tgactaccat tttgtcacgc atcaatctgg tgttcattgt
     2521 gctatttact ggagagtgtg tactgaaact catctctcta cgccattatt attttaccat
     2581 tggatggaat atttttgatt ttgtggttgt cattctctcc attgtaggta tgtttcttgc
     2641 cgagctgata gaaaagtatt tcgtgtcccc taccctgttc cgagtgatcc gtcttgctag
     2701 gattggccga atcctacgtc tgatcaaagg agcaaagggg atccgcacgc tgctctttgc
     2761 tttgatgatg tcccttcctg cgttgtttaa catcggcctc ctactcttcc tagtcatgtt
     2821 catctacgcc atctttggga tgtccaactt tgcctatgtt aagagggaag ttgggatcga
     2881 tgacatgttc aactttgaga cctttggcaa cagcatgatc tgcctattcc aaattacaac
     2941 ctctgctggc tgggatggat tgctagcacc cattctcaac agtaagccac ccgactgtga
     3001 ccctaataaa gttaaccctg gaagctcagt taagggagac tgtgggaacc catctgttgg
     3061 aattttcttt tttgtcagtt acatcatcat atccttcctg gttgtggtga acatgtacat
     3121 cgcggtcatc ctggagaact tcagtgttgc tactgaagaa agtgcagagc ctctgagtga
     3181 ggatgacttt gagatgttct atgaggtttg ggagaagttt gatcccgatg caactcagtt
     3241 catggaattt gaaaaattat ctcagtttgc agctgcgctt gaaccgcctc tcaatctgcc
     3301 acaaccaaac aaactccagc tcattgccat ggatttgccc atggtgagtg gtgaccggat
     3361 ccactgtctt gatatcttat ttgcttttac aaagcgggtt ctaggagaga gtggagagat
     3421 ggatgctcta cgaatacaga tggaagagcg attcatggct tccaatcctt ccaaggtctc
     3481 ctatcagcca atcactacta ctttaaaacg aaaacaagag gaagtatctg ctgtcattat
     3541 tcagcgtgct tacagacgcc accttttaaa gcgaactgta aaacaagctt cctttacgta
     3601 caataaaaac aaaatcaaag gtggggctaa tcttcttata aaagaagaca tgataattga
     3661 cagaataaat gaaaactcta ttacagaaaa aactgatctg accatgtcca ctgcagcttg
     3721 tccaccttcc tatgaccggg tgacaaagcc aattgtggaa aaacatgagc aagaaggcaa
     3781 agatgaaaaa gccaaaggga aagactacaa agaccatgac ggtgattata aagatcatga
     3841 catcgactac aaggatgacg atgacaagta actcgagata atcaacctct ggattacaaa
     3901 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3961 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4021 ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc
     4081 tgctggacag gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt
     4141 tgtgatgcta ttgctttatt tgtaaccatc tagctttatt tgtgaaattt gtgatgctat
     4201 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4261 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagatctg gagaccggtg
     4321 tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
     4381 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     4441 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
     4501 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     4561 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4621 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4681 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4741 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     4801 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4861 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
     4921 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
     4981 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     5041 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     5101 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     5161 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     5221 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5281 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5341 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5401 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5461 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5521 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5581 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5641 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5701 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5761 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5821 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5881 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5941 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6001 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6061 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6121 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6181 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6241 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6301 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6361 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6421 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6481 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6541 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6601 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6661 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6721 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6781 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6841 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6901 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6961 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7021 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7081 tgt
//