| 质粒名称: | pRecE564T-kan-pBL1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002470 |
| 产品用途: | For gene editing in Corynebacterium glutamicum |
| 存储实验室: | 杨晟Sheng Yang Lab（CEMPS,CAS） |
|
载体骨架基本信息 |
|
| 载体名称: | pXMJ1pTRC |
| 表达系统: | 细菌表达 |
| 启动子: | Ptrc |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | RecE564T |
| 物种来源: | Escherichia coli |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
One-Step Multi-fragment Assembly and Targeted Genomic Integration in Corynebacterium glutamicum
ACS Synthetic Biology，，2026/03/13/
Xiaoyu Wang, Siqi Yang, Xiaojie Zhou, Jiao Zhu, Fenghui Qian, Yu Jiang, Sheng Yang
|
买家导航
pRecE564T-kan-pBL1载体质粒基本信息
pRecE564T-kan-pBL1质粒图谱载体图谱和pRecE564T-kan-pBL1载体序列质粒序列多克隆位点信息
pRecE564T-kan-pBL1质粒载体简介
pRecE564T-kan-pBL1质粒序列载体序列
LOCUS pRecE564T-kan-pBL1 8978 bp DNA circular SYN 20-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8978)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8978
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 1..8
/mol_type="other DNA"
/db_xref="taxon:2908161"
/organism="Vector pVC-AmR-LacI-Ptrc-id2-RecE564T"
source 9..8978
/mol_type="other DNA"
/db_xref="taxon:2908161"
/organism="Vector pVC-AmR-LacI-Ptrc-id2-RecE564T"
rep_origin 1268..2714
/label=ori_pBL1
/note="ori_pBL1"
CDS complement(3289..4371)
/codon_start=1
/transl_table=11
/product="LacI"
/label=LacI
/db_xref="GI:2173909417"
/protein_id="UIG83382.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(4372..4449)
/gene="lacI"
/label=lacI promoter
regulatory 4748..4849
/label=Ptrc-id2
/note="Ptrc-id2"
/regulatory_class="promoter"
protein_bind 4815..4831
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 4881..5795
/codon_start=1
/transl_table=11
/product="RecE654"
/label=RecE564
/note="truncated variant of Rac prophage RecE protein"
/db_xref="GI:2173909418"
/protein_id="UIG83383.1"
/translation="MEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNEN
YHAGPGISKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFI
VAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESA
GHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAF
YSDGYEAQFGVQPTFVFLVASTTIECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLSDCL
NTDEWPAIKTLSLPRWAKEYAND"
CDS 5788..6597
/codon_start=1
/transl_table=11
/product="RecT"
/label=RecT
/note="Rac prophage RecT protein"
/db_xref="GI:2173909419"
/protein_id="UIG83384.1"
/translation="MTKQPPIAKADLQKTQGNRAPAAVKNSDVISFINQPSMKEQLAAA
LPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLPFG
NKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIHRPG
ENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSLSKAGNNGPWVTHWEEMAKKTAI
RRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
regulatory 6662..6705
/label=T674
/note="T674"
/regulatory_class="terminator"
CDS 7359..8153
/codon_start=1
/gene="aph(3')-IIa"
/product="Aph(3')-IIa"
/label=aph(3')-IIa
/note="aminoglycoside phosphotransferase"
/note="/vntifkey=4"
/db_xref="GI:29164938"
/protein_id="AAO65197.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
rep_origin complement(8345..8933)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tccgtcgaac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg
61 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacagc ttgtctacgt
121 ctgatgcttt gaatcggacg gacttgccga tcttgtatgc ggtgattttt ccctcgtttg
181 cccacttttt aatggtggcc ggggtgagag ctacgcgggc ggcgacctgc tgcgctgtga
241 tccaatattc ggggtcgttc actggttccc ctttctgatt tctggcatag aagaaccccc
301 gtgaactgtg tggttccggg ggttgctgat ttttgcgaga cttctcgcgc aattccctag
361 cttaggtgaa aacaccatga aacactaggg aaacacccat gaaacaccca ttagggcagt
421 agggcggctt cttcgtctag ggcttgcatt tgggcggtga tctggtcttt agcgtgtgaa
481 agtgtgtcgt aggtggcgtg ctcaatgcac tcgaacgtca cgtcatttac cgggtcacgg
541 tgggcaaaga gaactagtgg gttagacatt gttttcctcg ttgtcggtgg tggtgagctt
601 ttctagccgc tcggtaaacg cggcgatcat gaactcttgg aggttttcac cgttctgcat
661 gcctgcgcgc ttcatgtcct cacgtagtgc caaaggaacg cgtgcggtga ccacgacggg
721 cttagccttt gcctgcgctt ctagtgcttc gatggtggct tgtgcctgcg cttgctgcgc
781 ctgtagtgcc tgttgagctt cttgtagttg ctgttctagc tgtgccttgg ttgccatgct
841 ttaagactct agtagctttc ctgcgatatg tcatgcgcat gcgtagcaaa cattgtcctg
901 caactcattc attatgtgca gtgctcctgt tactagtcgt acatactcat atttacctag
961 tctgcatgca gtgcatgcac atgcagtcat gtcgtgctaa tgtgtaaaac atgtacatgc
1021 agattgctgg gggtgcaggg ggcggagcca ccctgtccat gcggggtgtg gggcttgccc
1081 cgccggtaca gacagtgagc accggggcac ctagtcgcgg ataccccccc taggtatcgg
1141 acacgtaacc ctcccatgtc gatgcaaatc tttaacattg agtacgggta agctggcacg
1201 catagccaag ctaggcggcc accaaacacc actaaaaatt aatagttcct agacaagaca
1261 aacccccgtg cgagctacca actcatatgc acgggggcca cataacccga aggggtttca
1321 attgacaacc atagcactag ctaagacaac gggcacaaca cccgcacaaa ctcgcactgc
1381 gcaaccccgc acaacatcgg gtctaggtaa cactgagtaa cactgaaata gaagtgaaca
1441 cctctaagga accgcaggtc aatgagggtt ctaaggtcac tcgcgctagg gcgtggcgta
1501 ggcaaaacgt catgtacaag atcaccaata gtaaggctct ggcggggtgc cataggtggc
1561 gcagggacga agctgttgcg gtgtcctggt cgtctaacgg tgcttcgcag tttgagggtc
1621 tgcaaaactc tcactctcgc tgggggtcac ctctggctga attggaagtc atgggcgaac
1681 gccgcattga gctggctatt gctactaaga atcacttggc ggcgggtggc gcgctcatga
1741 tgtttgtggg cactgttcga cacaaccgct cacagtcatt tgcgcaggtt gaagcgggta
1801 ttaagactgc gtactcttcg atggtgaaaa catctcagtg gaagaaagaa cgtgcacggt
1861 acggggtgga gcacacctat agtgactatg aggtcacaga ctcttgggcg aacggttggc
1921 acttgcaccg caacatgctg ttgttcttgg atcgtccact gtctgacgat gaactcaagg
1981 cgtttgagga ttccatgttt tcccgctggt ctgctggtgt ggttaaggcc ggtatggacg
2041 cgccactgcg tgagcacggg gtcaaacttg atcaggtgtc tacctggggt ggagacgctg
2101 cgaaaatggc aacctacctc gctaagggca tgtctcagga actgactggc tccgctacta
2161 aaaccgcgtc taaggggtcg tacacgccgt ttcagatgtt ggatatgttg gccgatcaaa
2221 gcgacgccgg cgaggatatg gacgctgttt tggtggctcg gtggcgtgag tatgaggttg
2281 gttctaaaaa cctgcgttcg tcctggtcac gtggggctaa gcgtgctttg ggcattgatt
2341 acatagacgc tgatgtacgt cgtgaaatgg aagaagaact gtacaagctc gccggtctgg
2401 aagcaccgga acgggtcgaa tcaacccgcg ttgctgttgc tttggtgaag cccgatgatt
2461 ggaaactgat tcagtctgat ttcgcggtta ggcagtacgt tctagattgc gtggataagg
2521 ctaaggacgt ggccgctgcg caacgtgtcg ctaatgaggt gctggcaagt ctgggtgtgg
2581 attccacccc gtgcatgatc gttatggatg atgtggactt ggacgcggtt ctgcctactc
2641 atggggacgc tactaagcgt gatctgaatg cggcggtgtt cgcgggtaat gagcagacta
2701 ttcttcgcac ccactaaaag cggcataaac cccgttcgat attttgtgcg atgaatttat
2761 ggtcaatgtc gcgggggcaa actatgatgg gtcttgttgt tgacaatggc tgatttcatc
2821 aggaatggaa ctgtcatgct gttatgtgcc tggctcctaa tcaaagctgg ggacaatggg
2881 ttgccccgtt gatctgatct agttcggatt ggcggggctt cactgtatct gggggtggca
2941 tcgtgaatag attgcacacc gtagtgggca gtgtgcacac catagtgggc atgagtaata
3001 cctacgcgcg cgtgggctag ggcttaacgc gcgttttgcc gtgctgcggg gcatacgtta
3061 gcgcatacgc ttttttctgt gaaacctttt tgtgttgttg tttcgtgttg gtttcctttc
3121 tgttggcggg gcaacttaac gcctgcgggg gtggttgttg acgttaacgg tgcctatgag
3181 atcttccgga atcgatggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga
3241 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
3301 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
3361 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
3421 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
3481 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
3541 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt
3601 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
3661 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
3721 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
3781 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
3841 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
3901 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
3961 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
4021 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
4081 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
4141 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
4201 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
4261 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
4321 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
4381 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
4441 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
4501 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
4561 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
4621 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
4681 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
4741 taagcttcgg ttctggcaaa tattctgaaa tgagctgttg acattgtgag cgctcacaat
4801 tataatgtgt ggaattgtga gcggataaca atttcacaca ggatctagag ctagctaatc
4861 tgagttgagg ttaaaaaaca atggaacatc cgcacaatga gaatgctggc agcgatccgc
4921 atcgcgattg cagtgatgaa actggcgaag tcgcagatcc cgtaatcgta gaagacatag
4981 agccaggtat ttattacgga atttcgaatg agaattacca cgcgggtccc ggtatcagta
5041 agtctcagct cgatgacatt gctgatactc cggcactata tttgtggcgt aaaaatgccc
5101 ccgtggacac cacaaagaca aaaacgctcg atttaggaac tgctttccac tgccgggtac
5161 ttgaaccgga agaattcagt aaccgcttta tcgtagcacc tgaatttaac cgccgtacaa
5221 acgccggaaa agaagaagag aaagcgtttc tgatggaatg cgcaagcaca ggaaaaacgg
5281 ttatcactgc ggaagaaggc cggaaaattg aactcatgta tcaaagcgtt atggctttgc
5341 cgctggggca atggcttgtt gaaagcgccg gacacgctga atcatcaatt tactgggaag
5401 atcctgaaac aggaattttg tgtcggtgcc gtccggacaa aattatccct gaatttcact
5461 ggatcatgga cgtgaaaact acggcggata ttcaacgatt caaaaccgct tattacgact
5521 accgctatca cgttcaggat gcattctaca gtgacggtta tgaagcacag tttggagtgc
5581 agccaacttt cgtttttctg gttgccagca caactattga atgcggacgt tatccggttg
5641 aaattttcat gatgggcgaa gaagcaaaac tggcaggtca acaggaatat caccgcaatc
5701 tgcgaaccct gtctgactgc ctgaataccg atgaatggcc agctattaag acattatcac
5761 tgccccgctg ggctaaggaa tatgcaaatg actaagcaac caccaatcgc aaaagccgat
5821 ctgcaaaaaa ctcagggaaa ccgtgcacca gcagcagtta aaaatagcga cgtgattagt
5881 tttattaacc agccatcaat gaaagagcaa ctggcagcag ctcttccacg ccatatgacg
5941 gctgaacgta tgatccgtat cgccaccaca gaaattcgta aagttccggc gttaggaaac
6001 tgtgacacta tgagttttgt cagtgcgatc gtacagtgtt cacagctcgg acttgagcca
6061 ggtagcgccc tcggtcatgc atatttactg ccttttggta ataaaaacga aaagagcggt
6121 aaaaagaacg ttcagctaat cattggctat cgcggcatga ttgatctggc tcgccgttct
6181 ggtcaaatcg ccagcctgtc agcccgtgtt gtccgtgaag gtgacgagtt tagcttcgaa
6241 tttggccttg atgaaaagtt aatacaccgc ccgggagaaa acgaagatgc cccggttacc
6301 cacgtctatg ctgtcgcaag actgaaagac ggaggtactc agtttgaagt tatgacgcgc
6361 aaacagattg agctggtgcg cagcctgagt aaagctggta ataacgggcc gtgggtaact
6421 cactgggaag aaatggcaaa gaaaacggct attcgtcgcc tgttcaaata tttgcccgta
6481 tcaattgaga tccagcgtgc agtatcaatg gatgaaaagg aaccactgac aatcgatcct
6541 gcagattcct ctgtattaac cggggaatac agtgtaatcg ataattcaga ggaataattc
6601 agcctggcgg tgtaatgccc atggactatc gctaatcgta atcgcggaga tccagagtaa
6661 caaaaataac gccccacctt gataatgagg tggggcgtta tttttgtgtc ttaatccagt
6721 aggtagtctg ccaagatcag gttagtgcag taccaattgt tgttcttgga gaagttaaca
6781 gcctctgcca tactcaacgc aatgtcctta tcactggtct tagtgttcac gtaccgtacc
6841 cggcctgtgg tcatttcgat ctgtctcata acccaattct ctggatccgg gaccgagctc
6901 gaattacgtc gcggtgagtt caggcttttt catatctcat tgcccccgga cgagcgtctg
6961 ctccgccatt cgccgtccgc cgtgccaatc ggatcagccg tccaatcctt gggccacgcc
7021 cacccggcga tcatcgaggc ggtcaccgga agcggaacac gtagaaagcc agtccgcaga
7081 aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa
7141 gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg
7201 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
7261 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat
7321 caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
7381 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
7441 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
7501 ttgtcaagac cgacctgtcc ggtgccctga atgaactcca agacgaggca gcgcggctat
7561 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
7621 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
7681 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
7741 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
7801 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
7861 ccgaactgtt cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc gtcgtgaccc
7921 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
7981 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
8041 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
8101 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagaatgag
8161 cttcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
8221 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
8281 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
8341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
8401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
8461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
8521 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
8581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
8641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
8701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
8761 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
8821 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
8881 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
8941 ctttgatctt ttctacgggg tctgacgctc agtggaac
//
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