| 质粒名称: | pAAV-CAG-Asp-RGEPO1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000741 |
| 产品用途: | Expressing the red potassium indicator on the cell surface of cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 中拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Asp-RGEPO1 |
| 物种来源: | Bacterial |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo.
Yang L., Pathiranage V., Zhou S., Sun X., Zhang H., Lai C., Gu C., Subach F.V., Drobizhev M., Walker A.R., Piatkevich K.D.
PLOS Biol. 23, e3002993 (2025)
|
pAAV-CAG-Asp-RGEPO1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-Asp-RGEPO1载体质粒基本信息
pAAV-CAG-Asp-RGEPO1质粒图谱载体图谱和pAAV-CAG-Asp-RGEPO1载体序列质粒序列多克隆位点信息
pAAV-CAG-Asp-RGEPO1质粒载体简介
pAAV-CAG-Asp-RGEPO1质粒序列载体序列
LOCUS pAAV-CAG-Asp-RGEPO1 6073 bp DNA circular SYN 22-OCT-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6073)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6073
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(160..748)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(919..1779)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1780..1884)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1910..2365)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2904..3181
/label=chicken beta-actin promoter
primer_bind 3581..3629
/label=Asp-cag-F1
primer_bind 3581..3613
/label=Azu-cag-F1
primer_bind 3581..3613
/label=Lucy-cag-F1
primer_bind 3582..3614
/label=Intra-cag-F1
misc_feature 3605..3610
/label=kozak
CDS 3611..3682
/codon_start=1
/label=Asp-tag
/translation="MASSLYSFLLALSIVYIFVAPTHS"
CDS 3683..3709
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind 3705..3749
/label=PD-F1
CDS 3731..4183
/codon_start=1
/label=N-mApple
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
FQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMSF
EDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGWEAS"
primer_bind 3731..3748
/label=N-mapple-F2
primer_bind 3731..3743
/label=Intra-F1
primer_bind 3731..3743
/label=Intra-cag-F1
primer_bind 4157..4204
/label=FR-1-3-F
primer_bind 4157..4178
/label=FR-Y-Ra-F
primer_bind complement(4166..4186)
/label=mApple-N-R1
primer_bind complement(4170..4183)
/label=N-mapple-R
primer_bind complement(4172..4190)
/label=FR-1-3-R
primer_bind 4180..4219
/label=PA-S9A-F1
primer_bind 4181..4213
/label=PA-F6A-F1
CDS 4184..4186
/codon_start=1
/label=LINKER1
/translation="P"
primer_bind complement(4185..4234)
/label=F6A-R1
CDS 4187..4618
/codon_start=1
/label=YgaU_Hydrothermal
/translation="GIWDFVKSAGRKIGIGSEEAAPAADDLKKELDSYKLGTDDVKVEV
VGDKAVITGNVADQSILEKAIVAVGNTLGISKVESNVEVPDAKEPVFHTVKKGDTLWAI
ASKQYGNVSKYNAIFEANKPMLSHPDKIYPGQVLRIPDLG"
primer_bind complement(4190..4234)
/label=V7A-R1
primer_bind complement(4193..4234)
/label=K8A-R1
primer_bind complement(4197..4234)
/label=S9A-R1
misc_feature 4199..4219
/label=Mutant
primer_bind complement(4204..4234)
/label=G11A-R1
primer_bind complement(4204..4234)
/label=R12A-R1
primer_bind 4220..4237
/label=0811-F2
primer_bind 4377..4409
/label=PA-T73A-F1
primer_bind complement(4381..4432)
/label=G71A-R1
primer_bind 4381..4415
/label=PA-G75A-F1
primer_bind complement(4381..4398)
/label=0905-R2
primer_bind complement(4382..4432)
/label=N72A-R1
primer_bind complement(4385..4432)
/label=T73A-R1
misc_feature 4394..4417
/label=Mmutant
primer_bind complement(4396..4432)
/label=G75A-R1
primer_bind complement(4397..4432)
/label=I76A-R1
primer_bind complement(4399..4432)
/label=S77A-R1
primer_bind complement(4404..4433)
/label=K78A-R1
primer_bind complement(4406..4432)
/label=L74A-R1
primer_bind 4418..4436
/label=0811-F1
primer_bind complement(4510..4534)
/label=G113V-R
primer_bind complement(4595..4611)
/label=Ec-kbp-R
primer_bind 4619..4637
/label=mApple-C-F1
CDS 4619..4627
/codon_start=1
/label=LINKER
/translation="SAC"
CDS 4628..4885
/codon_start=1
/label=C-mApple
/translation="VSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLP
GAYIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYK"
primer_bind 4628..4648
/label=C-mApple-F2
primer_bind complement(4818..4837)
/label=FR-R
primer_bind complement(4867..4906)
/label=PD-R1
primer_bind complement(4869..4885)
/label=Intra-cag-R1
primer_bind 4871..4887
/label=0821_KA_FRCaMPi_F
primer_bind 4886..4906
/label=MYC-F1
CDS 4895..4924
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 4928..5074
/codon_start=1
/product="transmembrane domain from platelet derived growth
factor receptor beta"
/label=PDGFR-beta TM domain
/translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
KKPR"
primer_bind complement(5057..5100)
/label=Asp-syn-R1
/note="C08"
primer_bind complement(5057..5099)
/label=Azu-cag-R1
primer_bind complement(5075..5099)
/label=Intra-cag-R1
misc_feature 5102..5690
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(5573..5584)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 5733..5854
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
61 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
601 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
1021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
1081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
1141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
1201 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
1261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
1321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
1381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
1441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
1501 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
1561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
1621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
1681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
1741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
1801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
1861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
1921 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
1981 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
2041 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
2101 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
2161 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
2221 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
2281 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
2341 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
2401 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgc gcgctcgctc gctcactgag
2461 gccgcccggg caaagcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag
2521 cgagcgcgca gagagggagt ggccaactcc atcactaggg gttccttgta gttaatgatt
2581 aacccgccat gctacttatc tacgtagcca tgctctagga agatcgtagg ttaattaagg
2641 cgcgccgcct aggccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
2701 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
2761 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
2821 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
2881 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
2941 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
3001 gatgggggcg gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
3061 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
3121 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
3181 gggagtcgct gcgacgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
3241 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
3301 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
3361 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggctgtccg
3421 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
3481 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
3541 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ggggatccgg
3601 taccgccacc atggccagca gcctgtacag cttcctgctg gccctgagca tcgtgtacat
3661 cttcgtggcc cccacccaca gctatccata tgatgttcca gattatgctg gggcccagcc
3721 ggccagatct atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
3781 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
3841 gggcgagggc cgcccctacg aggccttcca gaccgctagg ctgaaggtga ccaagggtgg
3901 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
3961 cattaagcac ccagccgaca tccccgacta cttcaagctg tccttccccg agggcttcag
4021 gtgggagcgc gtgatgagct tcgaggacgg cggcattatt cacgttaatc aggactcctc
4081 cctgcaggac ggcgtattca tctacaaggt gaagctgctc ggcaccaact tcccccccga
4141 cggccccgta atgcagaaga agaccatggg ctgggaggct tcccccggca tctgggactt
4201 cgtgaagagc gccggcagga agatcggcat cggcagcgag gaggccgccc ccgccgccga
4261 cgacctgaag aaggagctgg acagctacaa gctgggcacc gacgacgtga aggtggaggt
4321 ggtgggcgac aaggccgtga tcaccggcaa cgtggccgac cagagcatcc tggagaaggc
4381 catcgtggcc gtgggcaaca ccctgggcat cagcaaggtg gagagcaacg tggaggtgcc
4441 cgacgccaag gagcccgtgt tccacaccgt gaagaagggc gacaccctgt gggccatcgc
4501 cagcaagcag tacggcaacg tcagcaagta caacgccatc ttcgaggcca acaagcccat
4561 gctgagccac cccgacaaga tctaccccgg ccaggtgctg aggatccccg acctgggctc
4621 tgcctgcgtt tccgagtgga tgtaccccga ggacggcgcc ctgaagagcg agatcaggaa
4681 ggggctgagg ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc
4741 caagaagccc gtgcagctgc ccggcgccta catcgtcgac atcgagttgg acatcttgtc
4801 ccacaacgag gactacacca tcgtggaaca gtgcgaacgc gccgtgggcc gccaccccac
4861 cggtggcacg gacgcgctgt acaagggcgt cgacgaacaa aaactcatct cagaagagga
4921 tctgaatgct gtgggccagg acacgcagga ggtcatcgtg gtgccacact ccttgccctt
4981 taaggtggtg gtgatctcag ccatcctggc cctggtggtg ctcaccatca tctcccttat
5041 catcctcatc atgctttggc agaagaagcc acgttaggaa ttcgatatca agcttatcga
5101 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
5161 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
5221 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
5281 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
5341 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
5401 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
5461 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
5521 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
5581 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
5641 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
5701 tcgacccggg cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
5761 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
5821 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
5881 gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
5941 tggtaaaatc gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt
6001 taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
6061 gctcgctcac tga
//
pAAV-CAG-Asp-RGEPO1载体图谱质粒图谱pdf版和pAAV-CAG-Asp-RGEPO1载体序列质粒序列等相关资料下载
pAAV-CAG-Asp-RGEPO1质粒载体应用举例
- 上一篇：pKeratin-tdStayGold
- 下一篇：没有了
