| 质粒名称: | pSF11-wmCardinal |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000107 |
| 产品用途: | Expresses mCardinal in C. elegans neurons |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pSF11 |
| 表达系统: | 线虫表达 |
| 启动子: | tag168 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mCardinal |
| 物种来源: | Synthetic |
| 密码子优化: | 蠕虫表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
买家导航
pSF11-wmCardinal载体质粒基本信息
pSF11-wmCardinal质粒图谱载体图谱和pSF11-wmCardinal载体序列质粒序列多克隆位点信息
pSF11-wmCardinal质粒载体简介
pSF11-wmCardinal质粒序列载体序列
LOCUS pSF11-wmCardinal 6931 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6931)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6931
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 54..2717
/label=tag-168 promoter
primer_bind 2796..2816
/label=pSF11-Fw-Seq
primer_bind 2808..2858
/label=0507-628-F
misc_feature 2834..2837
/label=optimized sequence for ribosomal binding site
misc_feature 2834..2837
/label=codon adapted insert for smGFP-myc
/note="codon adapted by
https://worm.mpi-cbg.de/codons/cgi-bin/optimize.py
Intron * 2 (3 requested, but 1 is inside myc regions and
thus removed)
CAI set to 0.60
Check all 3 boxes
"
CDS 2838..3671
/codon_start=1
/label=wmCardinal
/translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQVSLNIYILTNPDYLNFQGIPDFFKQS
FPEGFTWERVTTYEDGGVLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEA
TTETLYPADGGLEGRCDMALKLVGGGHLHCNLKVSLNSSVLTNHTYLNFQTTYRSKKPA
KNLKMPGVYFVDRRLERIKEADNETYVEQHEVAVARYCDLPSKLGHKLNGMDELYK"
misc_feature 3072..3122
/label=intron-1
misc_feature 3426..3476
/label=intron-2
primer_bind complement(3716..3732)
/label=pSF11-Rv-Seq
promoter 4810..4914
/gene="bla"
/label=AmpR promoter
CDS 4915..5775
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5870..6552
/label=ColE1 origin
protein_bind 6822..6843
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6858..6888
/label=lac promoter
/note="promoter for the E. coli lac operon"
misc_binding 6892..6914
/label=LacO
protein_bind 6896..6912
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind join(6920..6931,1..5)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
181 aaaaaaaaaa gtgagggggc tcactgcccc cccccccctc ccccccccct cttctccttg
241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
601 ttttttgaac ttttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
901 aattctgaga tgtcttagtg catttttcgg gcatacataa aaaaaaattt atcttaataa
961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
1201 aaaaaaaaaa acctctttta ttaattttgt gctccaaact ttcccacggt acgcaaatca
1261 tgtaatatgt acttttaaac attatttttc caatcaaaaa tatccaacag cttttcctta
1321 caatttgaaa atttttatta aaatttcaaa aaaatttttt ttttttttta atttcgaatt
1381 tttttaaact tgttgtaact tttttccctg tcggcgccaa ttaacaaaat acgtagaatt
1441 ttgcgcttat tgttttgtta gttaacaaat ttttgacaac tttcttatca gctgagattt
1501 aggtgtttca gttcgcaatc tgcgtctctc cttgctctcc tctattttaa aacccccata
1561 actttgttct tgtgagagtt atcaaaaaat tgtcaactaa caaaatatag agcacaaaaa
1621 tatgcacatt ttgtatgttg acaatttttt gatacctttt ttagttttcg agatatctta
1681 gttttagttt agctgtacct ttttctggca aaaaacttcc aaatttcggc aatttttcgg
1741 caaatttccc ggcaaatttc gccaagtaca tcgggctctc agacaagtgg tctgtgagtg
1801 gaagtcgata aaagtagttt acgtgctctc cgtccccccg cttcttctcc gtctctgctt
1861 ctatttctcc attgactagt cccactactt tttacggggc ggagggctct cttgcactcc
1921 agcttcttct ctttttgtgc tcttttttgc ccgtatgtgg gtgctcattt atttttcttt
1981 ggttttaaaa taaattcggt ttattaggct actcgtaaaa atgaagaaaa aacttcaaat
2041 tcaaacgaca ttaaaaaaaa atttaataaa aattttttga attgctcaaa agccccacct
2101 atatatatat atagctccgc ctacttctcg cacagacccc gcccactttt tgactccgcc
2161 cccttattgg caaaagttct tgaaaaaaat aaaaataaaa attcaaattg aaacaatttt
2221 tttttaatga agtagttttt ttctaaattg atgaaaattt ctaaacagtt tacaagcacc
2281 gcccactttt tgactccgcc cctttgttgg aaaaattctt gtaaaaaaaa ataaaaaata
2341 aaagcttctc tttcaaacga tttttagttg gtttttgtat gaaaagtttt tttcctaaaa
2401 tagaaatgtc tgaataattt gcaagctccg cccttttctt acccagactc cgccccttta
2461 ttgactaatt tttttttgtc catttacctg aagttcaaaa aaaaaattta cagtttcata
2521 gagtaataat ttttcaaaaa tctctttttc caatgtttaa tgtgtcatta taaacccgcc
2581 cagttttaag ctccgcccat ttaggccccg cccatttatg acccaaatat ttttttctct
2641 attcacccgt ggatttccct ccaacaagtt accccaaaaa taaaaataaa aattcctgca
2701 ggcgcccacc gggatccggc gcgcctctag aggatccccg ggattggcca aaggacccaa
2761 aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag
2821 cgtcgacggt accaaaaatg gtctccaagg gagaggagct tatcaaggag aacatgcaca
2881 tgaagcttta catggaggga accgtcaaca accaccactt caagtgcacc accgagggag
2941 agggaaagcc atacgaggga acccaaaccc aacgcatcaa ggtcgtcgag ggaggaccac
3001 ttccattcgc cttcgacatc cttgccacct gcttcatgta cggatccaag accttcatca
3061 accacaccca ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
3121 agggaatccc agacttcttc aagcagtcct tcccagaggg attcacctgg gagcgcgtca
3181 ccacctacga ggacggagga gtccttaccg tcacccaaga cacctccctt caagacggat
3241 gccttatcta caacgtcaag cttcgtggag tcaacttccc atccaacgga ccagtcatgc
3301 agaagaagac ccttggatgg gaggccacca ccgagaccct ttacccagcc gacggaggac
3361 ttgagggacg ctgcgacatg gcccttaagc ttgtcggagg aggacacctt cactgcaacc
3421 ttaaggtaag tttaaacagt tcggtactaa ctaaccatac atatttaaat tttcagacca
3481 cctaccgctc caagaagcca gccaagaacc ttaagatgcc aggagtctac ttcgtcgacc
3541 gccgccttga gcgtatcaag gaggccgaca acgagaccta cgtcgagcaa cacgaggtcg
3601 ccgtcgcccg ttactgcgac cttccatcca agcttggaca caagcttaac ggaatggacg
3661 agctttacaa gtaagagctc cgcatcggcc gctgtcatca gatcgccatc tcgcgcccgt
3721 gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc
3781 tcccaccccc tatttttgtt attatcaaaa aaacttcttc ttaatttctt tgttttttag
3841 cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt
3901 aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa
3961 tctacacaat gttctgtgta cacttcttat gtttttttta cttctgataa attttttttg
4021 aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg
4081 accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg
4141 tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat
4201 tttcctgctt ttgctttttg ggggtttccc ctattgtttg tcaagagttt cgaggacggc
4261 gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg
4321 tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg
4381 tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt
4441 tcctactagt cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
4501 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
4561 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
4621 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
4681 atgcgtaagg agaaaatacc gcatcaggcg gccttaatta agggcctcgt gatacgccta
4741 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
4801 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
4861 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
4921 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
4981 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
5041 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
5101 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
5161 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
5221 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
5281 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
5341 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
5401 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
5461 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
5521 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
5581 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
5641 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
5701 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
5761 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
5821 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
5881 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
5941 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
6001 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
6061 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
6121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
6181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
6241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
6301 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
6361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
6421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
6481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
6541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
6601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
6661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
6721 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
6781 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
6841 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
6901 agcggataac aatttcacac aggaaacagc t
//
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pSF11-wmCardinal质粒载体应用举例
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