| 质粒名称: | pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001189 |
| 产品用途: | in vivo |
| 存储实验室: | Yuxuan Guo Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒表达 |
| 启动子: | hTNNT2 |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
|
插入基因信息 |
|
| 插入基因名称: | luciferase |
| 物种来源: | MUS |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Neural Organoids Protect Engineered Heart Tissues From Glucolipotoxicity by Transferring Versican in a Co-Culture System.
Bai B., Li J., Wang Z., Yang Y., He J., Chen G., Zhang Y., Qi Y., Wan Z., Cai L., Wang R., Wang K., Zhao D., Zhang J., Huang W., Xu X.R., Sun M., Han X., Liu Y., Zhang D., Zhu W., Liu J., Guo Y.
Cell Prolif. 0, e70070 (2025)
|
买家导航
pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122载体质粒基本信息
pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122质粒图谱载体图谱和pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122载体序列质粒序列多克隆位点信息
pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122质粒载体简介
pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122质粒序列载体序列
LOCUS pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122 7064 bp ds-DNA circular 21-JUN-2024
DEFINITION .
COMMENT ^^
FEATURES Location/Qualifiers
misc_feature 1..130
/label="5' ITR"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
misc_feature 29..34
/label="XmaI"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 40..45
/label="XmaI"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
misc_feature 176..197
/label="overlap"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
primer 176..218
/label="cmv2TNT4-clone-F"
/note="sequence: TGGGGATCCTCTAGAACTATAGCTCAGTCCATTAGGAGCCAGT"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 178..197
/label="nhe1cut"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 198..218
/label="overlap"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 198..741
/label="hTNT promoter"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
misc_feature 737..756
/label="cut of not1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
primer 737..777
/label="tnt4-zn-clone-F"
/note="sequence: GGCAGagctCCTCGAGAAGcACTGACCAACAGCGTGAAGAC"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 757..777
/label="overlap"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 757..795
/label="upstream"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
exon 757..795
/label="Zfr-201 Exon 19"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
gene 757..1989
/label="Zfr minigene(1233BP)"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
primer 769..792
/label="Zfr-rtpcr-F"
/note="sequence: CGTGAAGACATTACATCCAGTGCA"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 841..930
/label="I1D1"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 841..1200
/label="to delete"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 931..1020
/label="I1D2"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 1021..1110
/label="I1D3"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 1111..1200
/label="I1D4"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 1261..1264
/label="bp"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 1296..1379
/label="PSEX"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
CDS 1299..1379
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="KGLHIKNIV*TL*GQRSVLISPD*KL*"
primer 1345..1369
/label="Zfr-qpcr-F"
/note="sequence: GAAGTGTGCTCATTTCTCCCGATTA"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
CDS 1376..1379
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="M"
misc_feature 1380..1948
/label="keep"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
primer complement(1390..1431)
/label="tnt4-zn-clone-R"
/note="sequence: aaaccctgtttcaaaTCTCCCACCCCCAAAAGGCAAAATACC"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
misc_feature 1412..1431
/label="overlap"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
primer 1412..1453
/label="zn-luci-clone-F"
/note="sequence: GGAGAtttgaaacagggtttctccttacagctctggctttcc"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
misc_feature 1433..1522
/label="I2D1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 1433..1882
/label="to delete"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 1523..1612
/label="I2D2"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 1613..1702
/label="I2D3"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 1703..1792
/label="I2D4"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 1793..1882
/label="I2D5"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 1933..1937
/label="bp"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 1949..1989
/label="downstream"
/ApEinfo_revcolor="#b4abac"
/ApEinfo_fwdcolor="#b4abac"
exon 1949..1989
/label="Zfr-201 Exon 20"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
CDS 1951..1989
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="CIETPCIPSDTQS"
misc_feature 1966..1989
/label="overlap"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
misc_feature 1990..2046
/label="P2A"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
CDS 1990..2046
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="ATNFSLLKQAGDVEENPGP"
primer complement(2003..2025)
/label="P2A-R1"
/note="sequence: gtctccagcctgTttTagTaggc"
/ApEinfo_revcolor="#b4abac"
/ApEinfo_fwdcolor="#b4abac"
primer complement(2005..2029)
/label="P2A-R2"
/note="sequence: cAacgtctccagcctgTttTagTag"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 2007..2028
/label="non-AUG start codons removed"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
primer complement(2011..2031)
/label="P2A-R5"
/note="sequence: ctcAacgtctccagcctgTtt"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
primer complement(2017..2037)
/label="P2A-R3"
/note="sequence: gttctcctcAacgtctccagc"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
primer complement(2025..2044)
/label="P2A-R4"
/note="sequence: gtccagggttctcctcAacg"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
primer complement(2036..2077)
/label="zn-luci-clone-R"
/note="sequence: cttctGCGTAATCTGGAACGTCgTATGGATAaggtccagggt"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
CDS 2047..2070
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="YPYDVPDY"
misc_feature 2047..2073
/label="HA tag"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
CDS 2047..2098
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="YPYDVPDYAEDAKNIKK"
misc_feature 2059..2077
/label="OVERLAP"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
primer 2059..2101
/label="ha-luci-clone-F"
/note="sequence: GTTCCAGATTACGCagaagacgccaaaaacataaagaaaggcc"
/ApEinfo_revcolor="#b7e6d7"
/ApEinfo_fwdcolor="#b7e6d7"
CDS 2074..2086
/label="Translation 2074-2086"
/translation="EDAK"
CDS 2074..3722
/label="luciferase"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
/note="EC_number: product: firefly luciferase codon_start: 1 translation: MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV* transl_table: 1 gene: luc+ note: enhanced luc+ version of the luciferase gene protein_id: "
CDS 2074..3722
/label="Translation 2074-3722"
/translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKMPC"
primer complement(2169..2188)
/label="DM-St-luci-qPCR-R1"
/note="sequence: ttgttccaggaaccagggcg"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
primer complement(2541..2584)
/label="HA-luci-clone-R"
/note="sequence: gatgagatgtgacgaacgtgtacatcgactgaaatccctggtaa"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 2566..2584
/label="cut of bsrg1"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
primer 3082..3104
/label="luciferase-F"
/note="sequence: caaggatatgggctcactgagac"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
misc_feature 3082..3303
/label="qpcr-sequence"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
primer complement(3190..3207)
/label="Lucifer-seq3"
/note="sequence: cccggtatccagatccac"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
primer complement(3286..3303)
/label="luciferase-R"
/note="sequence: caaggcgttggtcgcttc"
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
misc_feature 3704..3722
/label="overlap"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 3715..3734
/label="cut"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
CDS 3715..3745
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="PCKYL*RMGT"
primer 3715..3755
/label="luci-mir122-clone-F"
/note="sequence: ccgtgtaaGTACCTCTAGAGgatggggactgaaagcttcgg"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 3723..3727
/label="KpnI"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 3735..3755
/label="ol"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
source 3753..3839
/label="source"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
/note="organism: synthetic DNA construct mol_type: other DNA"
misc_feature 3757..3778
/label="miR122"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 3785..3806
/label="miR122"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 3813..3834
/label="miR122"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 3835..3855
/label="ol"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
primer complement(3835..3875)
/label="mir122-clone-R"
/note="sequence: CGTCCTCGAGGCCGCCCGGGcggtatcgataagctTcggcg"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
misc_feature 3840..3844
/label="HindIII"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 3856..3861
/label="XmaI"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
misc_feature 3856..3875
/label="cut"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
terminator 3899..4025
/label="PolyA"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
misc_feature 4114..4243
/label="3' ITR"
/ApEinfo_revcolor="#b7e6d7"
/ApEinfo_fwdcolor="#b7e6d7"
misc_feature 4199..4204
/label="XmaI"
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
misc_feature 4210..4215
/label="XmaI"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
rep_origin 4420..4875
/label="f1 origin"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 4901..5005
/label="Amp Promoter"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 5006..5863
/label="Amp resistent"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
rep_origin 6037..6625
/label="origin"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
ORIGIN
1 CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT
61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
121 AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACCAG GGTAATGGGG
181 ATCCTCTAGA ACTATAGCTC AGTCCATTAG GAGCCAGTAG CCTGGAAGAT GTCTTTACCC
241 CCAGCATCAG TTCAAGTGGA GCAGCACATA ACTCTTGCCC TCTGCCTTCC AAGATTCTGG
301 TGCTGAGACT TATGGAGTGT CTTGGAGGTT GCCTTCTGCC CCCCAACCCT GCTCCCAGCT
361 GGCCCTCCCA GGCCTGGGTT GCTGGCCTCT GCTTTATCAG GATTCTCAAG AGGGACAGCT
421 GGTTTATGTT GCATGACTGT TCCCTGCATA TCTGCTCTGG TTTTAAATAG CTTATCTGAG
481 CAGCTGGAGG ACCACATGGG CTTATATGGC GTGGGGTACA TGTTCCTGTA GCCTTGTCCC
541 TGGCACCTGC CAAAATAGCA GCCAACAccc cccaccccca ccgccatccc cctgccccac
601 ccgtccccTG TCGCACATTC CTCCCTCCGC AGGGCTGGCT CACCAGGCCC CAGCCCACAT
661 GCCTGCTTAA AGCCCTCTCC ATCCTCTGCC TCACCCAGTC CCCGCTGAGA CTGAGCAGAC
721 GCCTCCAGGA TCTGTCGGCA GagctCCTCG AGAAGcACTG ACCAACAGCG TGAAGACATT
781 ACATCCAGTG CACAGGTAAT TGAATTCTTG TTGGATTACT GTCTCTAAGG CCTGGGAGTT
841 TTATAATTGT TTCTTTATGT GTAATAATAA CCGTACTAGT CATTGTGATA CTTATTTGGG
901 AAGTAAGCCT TTAAAGAGGT TATTAAAAAG ATTTACTGAT TCCCACACCG GAAGTTGCTC
961 TTCTAGCCGT GAAAGAAGGA CGGCGTGGTC TTCAGTTTGC TATGTCCACT AACAATATGT
1021 CCGACCCACG AAGGCCCACC AAAGTTATAC TTGTGGCACA TATAAACCTT TGGAATCAAG
1081 GGACCTGATA CTGGATTATT AAATATTTAA GAGAATTTAT ATTTAATAAA CTTGATACTT
1141 CAGTGAGAAA GGACAAATCA TCAGTAGAAT GGTTTTGAGA TAGCTGATGA TTCTATTAAA
1201 AGTAGATATG TGTGTGGGTT AAATGACTTA AGCATACATC AAAAGACTGA GAAAGGCTCT
1261 TAACATTAAA AGATGGGCCt ttttttttCT TTTAGGTTAA AGGATTACAT ATTAAAAATA
1321 TTGTATAGAC TCTTTAAGGC CAAAGAAGTG TGCTCATTTC TCCCGATTAA AAGTTATGAG
1381 TAAGAATTGG GTATTTTGCC TTTTGGGGGT GGGAGAtttg aaacagggtt tctccttaca
1441 gctctggctt tcctggagca gtgaattgag cctggctcct ctgtaggagt aacgagttgc
1501 tttaaccact gacatctttc caccaccacc accaccCCTT ATGAATAGAC TAAATTTATT
1561 TAAAGATTGA TATTTTCTAG CTTAATTATT TGGAAAGCAA ATAAGCACAC AGAAAAGCCT
1621 CCTCCCCaaa aaaatataaa taaaaaaGTC TATCAGGACA TAGTATATGT CCCTTCTGAT
1681 TAATCATTGG TGGTGAAACA ACCCATTCAG GGTGTCTCTT CCATCACAGT GGAttttttt
1741 CATATTGTTA CATGGAGAAG TTAACTTAGT AAGAATTGAT GTGCTTCTGT GCCAGTGAGA
1801 TAGCTCTGCA GGTAAAAGGG AGACTGACCC CCAACTTGTA CTCTGGCCGT GGCAGTCATG
1861 TGCACACAAG TAAACTGAAT AAATATAAAG AGCAGATTTT AGAGTGTTTT GGCTTTAAAT
1921 TAATTCTATA TTCTAACTCT ATTTGCAGTT TGCATTGAGA CTCCTTGCAT TCCGTCAGAT
1981 ACACAAAGTg ctactaactt cagcctActA aaAcaggctg gagacgtTga ggagaaccct
2041 ggacctTATC CATAcGACGT TCCAGATTAC GCagaagacg ccaaaaacat aaagaaaggc
2101 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
2161 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
2221 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
2281 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
2341 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
2401 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
2461 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
2521 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
2581 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag
2641 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg
2701 cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa
2761 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
2821 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
2881 gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc
2941 ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
3001 gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg
3061 ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc agctattctg
3121 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
3181 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg
3241 tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
3301 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
3361 cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct
3421 cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca
3481 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
3541 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
3601 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
3661 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gatgccgtgt
3721 aaGTACCTCT AGAGgatggg gactgaaagc ttcggtcaaa caccattgtc acactccata
3781 ctatcaaaca ccattgtcac actccagatc aacaaacacc attgtcacac tccacgccgA
3841 agcttatcga taccgCCCGG GCGGCCTCGA GGACGGGGTG AACTACGCCT GAGGATCCGA
3901 TCTTTTTCCC TCTGCCAAAA ATTATGGGGA CATCATGAAG CCCCTTGAGC ATCTGACTTC
3961 TGGCTAATAA AGGAAATTTA TTTTCATTGC AATAGTGTGT TGGAATTTTT TGTGTCTCTC
4021 ACTCGGAAGC AATTCGTTGA TCTGAATTTC GACCACCCAT AATACCCATT ACCCTGGTAG
4081 ATAAGTAGCA TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA
4141 CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC
4201 CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCCTTAAT TAACCTAATT
4261 CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC
4321 GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC
4381 GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA CGCGCCCTGT AGCGGCGCAT
4441 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG
4501 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC
4561 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC
4621 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT
4681 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA
4741 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG
4801 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT
4861 TAACGCTTAC AATTTAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT
4921 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
4981 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC
5041 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA
5101 AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG
5161 TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT
5221 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG
5281 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC
5341 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC
5401 GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA
5461 CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC
5521 AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT
5581 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA
5641 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA
5701 ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA
5761 GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA
5821 TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT
5881 TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT
5941 GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG
6001 AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT
6061 AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA
6121 AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC
6181 TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC
6241 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT
6301 TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG
6361 GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA
6421 GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT
6481 AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA
6541 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC
6601 GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
6661 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
6721 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
6781 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
6841 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
6901 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
6961 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
7021 CTATGACCAT GATTACGCCA GATTTAATTA AGGCCTTAAT TAGG
//
pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122载体图谱质粒图谱pdf版和pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122载体序列质粒序列等相关资料下载
pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122质粒载体应用举例
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