pENTR4-Dual

2000 2300 2600 3000 询价

价格：元

联系方式：I47-825O-882O

买家导航

pENTR4-Dual载体质粒基本信息

出品公司:	Invitrogen
载体名称:	pENTR4-Dual
质粒类型:	Gateway载体；入门载体
高拷贝/低拷贝:	高拷贝
启动子:	无
克隆方法:	限制性内切酶；多克隆位点
载体大小:	3757 bp
5' 测序引物及序列:	pENTR-F: CTACAAACTCTTCCTGTTAGTTAG
3' 测序引物及序列:	pENTR-R: ATGGCTCATAACACCCCTTG
载体标签:	无
载体抗性:	卡那霉素和氯霉素
真核筛选标记:	无
备注:	pENTR4-Dual载体本身含有CCDb致死基因，能够使得普通的DH5alpha等细菌死亡，必须使用DB3.1感受态细胞进行质粒扩增；构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2 感受态细胞。
产品目录号:	--
病毒/非病毒:	非病毒

pENTR4-Dual质粒图谱载体图谱和pENTR4-Dual载体序列质粒序列多克隆位点信息

pENTR4-Dual载体图谱

pENTR4-Dual质粒载体简介

pENTR4-Dual质粒序列载体序列

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCTAGCATGGATCTCGGGGACGTCTAA
CTACTAAGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTT
TCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAAC
GTTGTGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAACTAAGC
AGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTTAGTTAGTTACTTAAGCT
CGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGC
TTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCACCATGGGAACCAATTCAGTCGACTGGATCC
GGTACCGAATTCGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGT
GGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGG
ATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAA
TGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACA
AGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAAT
GAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAA
ACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATG
TGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGC
CAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTT
TTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATG
CCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGC
GGGGCGTAATCTAGAGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTT
GCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGT
ATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAA
GGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATA
TTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCC
CCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGT
GTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCA
TTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACGAAT
TCGCGGCCGCACTCGAGATATCTAGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCT
TATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGCAGC
TCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAA
ACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCG
CGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAG
GTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAG
CGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACC
ATCAAGCATTTTATCCGTACTCCTGGTGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCAT
TCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCG
GTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAA
TCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAAC
AAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTC
ACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCA
GACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGC
TTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTT
TTTCTAATCAGAATTGGTTAATTGGTTGTAACATTATTCAGATTGGGCCCCGTTCCACTGAGCGTCAGAC
CCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAA
AAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAAC
TGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAG
AACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA
AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGG
GGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTA
TGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAG
GAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCT
CTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCG
GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

pFastBac1-His-C	pCRII-TOPO/22-1
pBad33	YEp16
pLC4-14	pHR31
pCold-SUMO	pIEx/Bac-3
pENTR-L1pLaclacZaL4	pENTR U6
pCEP4-DsRed2	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG
pAAVLINK-EF1a-5'-PXDN	FUW-tetO-hOCT4
pDM4	pAAVLINK-EF1a-HA-5'-ITSN1
pLenti6/V5-GW/lacZ	pUTK21
pGL4.18[luc2P/Neo]	pMCS-Green-Renilla-Luc
pSwitch	pFN10A (ACT)
pYC25	pAAVLINK-EF1a-HA-5'-DIP2C
pAAV-EFS-DIO-GRAB_OT1.0	pGEX-6p-1-hsG3BP2b
pENTR5'/CMVp	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG
pYES2-EGFP	pPM408
pBluescript II KS(-)	Pgex-HRV3C
pcDNA3.1+N-DYK-P2A	pcDNA3.1/Zeo(-)
pEYFP-Mem	pSP65-F1+
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG	pCEP4-Flag-N
pCDEF-HA-Ub-K63R	pHOOK1
pAP1(PMA)-TA-Luc	pQE-16
pGL4.21[luc2P/Puro]	pHY300PLK
YEp24	pBARMTE1
pBTB-5	pAAV-TRE-CAG-Fluc
pSEAP2-Basic	pIEX-3
pHT01	pMAL-p5x
pWY113 pET28a_AtmtE2	pIEX-1
pAMβ1	pShuttle-CMV
pcDNA4/TO/Myc-His/LacZ	pDEST8
YEp620	pAX01-ComK
pHBT-GFP-NOS	pAAVLINK-EF1a-HA-5-RALGAPB
pVK101	pNov.aro-bgl1-640
pRI101-AN	pHIL-D2
pSW510	pS-hydro_TA
pLVX-AmCyan1-N1	pBR327
pCRE-DD-AmCyan1	pRetroX-Tight-Hyg
pIRESpuro3	pGEX-6p-1-AT5G48650
pET28a-mOrange(M163A)	pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG
pGFP22	pBBR1MCS2-Tac-mCherry
ppyrG	pSuper.Retro.Neo
pUC19	pDNAPolbeta_TA
pL-brevisGH1-TA	pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG
pFR-Luc	pQLinkN
p19D+	pAAV-hSyn-Cepheid1s-WPRE
pDB262	pET-53-DEST
pIZT/V5-His	pcDNA6.2/nTC-Tag-DEST
pHisB-Sumo-NeMeHg	pAAV-CAG-NES-YTnC
pBABE-HAII	pMMB66EH
p1JI008	pcDNA3.1-CNTRFv1-HA-His
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG	pcDNA3.1-mRFP

AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-	pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3
pT7-SpCas9_sgRNA-site1 (RTW443)	pCI-6.20
pQCasTns(Vch)-entry(BsaI)	Double UP mNeon to mScarlet
pBLO 64.1	pCITEp65
pEG302 22aa SunTag NtDRMcd (no NLS) g4+g	pT7-hMLH1dn for IVT
pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA	pKAR2-Br512
Fuw-dCas9-Dnmt3a_IM	pGP-CMV-Gcamp6m
pAc-sgRNA-Cas9	pDriver
pShuttle-CMV-GFP U6promoter	murine TCR OTI-2A.pMIG II
pRK5-HA-Ubiquitin-K29R	pcDNA3.1-ERBB4
pLTR-RD114A	pAAV2/9n
pCMT-flp	Myc tag WT PTB
pRK5-HA-SH3BP4 W92A	pcDNA3.1_AktPH-mCherry
pLminP_Luc2P_RE4	PX551
pSI-Target-AID-NG	pEF1a-Usp9x_CD-mCherry
pcDNA3.1(+) CircRNA Mini Vector	GW1-Peredox-mCherry-NLS
pCMV-HA-JMJD3	AdEasier-1 cells (strain)
BB3cK_pGAP_23*_pTEF_Cas9	mAzurite-8xbiRhoBAST-24xMS2v6
SIRT6 Flag	pCDH-DCX-TurboID-V5-puro
GAG-CRErec	pcDNA3.1-TYMS
pBIG1a	pLenti-MP2
pKO (Hyg)	pEBTet_NAD-Snifit-mito
pQCXIP-GFP-LacR	pCMV-mA1-BE3
LeGO-iG	pGHYB
pGEX6p-1-BCCP-PupE	pHCMV_DUGV_GP
pCRISPR-cBEST	pcDNA5-T/O-Ds1-mCherry
pEGFP-C1 F-tractin-EGFP	pXR002:EF1a-dCasRx-2A-EGFP
pTA-HUH	pX330_SpCas9_USP7 Exon 3 gRNA
pAAVS1-PC-GCaMP6f	pNG9
MG1655 ΔendA ΔrecA (DE3)	GFP-MAP1B
pCDNA3 Flag p38 alpha	pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
pBiFC-VN155(I152L)	pET28GST-LIC
pJRH-1179 U6-reci Gag-Cas9 v2	pDawn-sfGFP
PA-GFPmt	pBK092
pAKTaq	p6XHis-T7(P266L)
pcDNA3.1 SIRT6_H133Y	pLIC-SM-HA
shYAP1	pINDUCER21-puro_Gateway-3xFLAG
EGFP-LC3	PB533_15F11-sfGFP
phage UbiC G3BP1-SNAP	pTM1_VP30_ZEBOV
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGre	pCI-neo.mCherry-NEDD4
BCR/ABL P190 transgenic construct	pET16B.Pfu
pMR-A-3	pscAAV-CAG-GFP
pBSK	pCS2+HyPer7-NLS
pHIV-Luciferase	mARG1 cassette 3
pQUAST>stop>mCD8-GFP	pPB-AMBRA1-3xFLAG
pMXs-hSOX2	CD109-bio-His
pCutamp	pcDNA3 Connexin43-GFP-APEX2
MP6	pMIH-TOMM20-Halo
CAMK2D gRNA (BRDN0001146112)	pRH2502
pCAG-FAST-p10	LentiGuide_DKO

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pENTR4-Dual

买家导航

pENTR4-Dual载体质粒基本信息

pENTR4-Dual质粒图谱载体图谱和pENTR4-Dual载体序列质粒序列多克隆位点信息

pENTR4-Dual质粒载体简介

pENTR4-Dual质粒序列载体序列

pENTR4-Dual载体图谱质粒图谱pdf版和pENTR4-Dual载体序列质粒序列等相关资料下载

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