| 质粒名称: | pGFP-EB1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 17234 |
| 存储实验室: | Lynne Cassimeris |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-N1 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4700 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | EB1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 800 bp |
| GenBank编号 | NM_012325 |
| 标签/融合蛋白: | GFP (C terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SacI（未销毁） |
| 3’克隆位点: | SmaI（未销毁） |
| 5’测序引物 | CMV-F |
| 3’测序引物 | EGFP-N |
|
文献引用方法 |
|
| 材料和方法部分: | pGFP-EB1 was a gift from Lynne Cassimeris (Addgene plasmid # 17234 ; http://n2t.net/addgene:17234 ; RRID:Addgene_17234) |
| 参考文献部分: | Organization and dynamics of growing microtubule plus ends during early mitosis. Piehl M, Cassimeris L. Mol Biol Cell. 2003 Mar . 14(3):916-25. 10.1091/mbc.e02-09-0607 PubMed 12631713 |
pGFP-EB1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
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买家导航
pGFP-EB1载体质粒基本信息
pGFP-EB1质粒图谱载体图谱和pGFP-EB1载体序列质粒序列多克隆位点信息
pGFP-EB1质粒载体简介
pGFP-EB1质粒序列载体序列
LOCUS pGFP-EB1 5540 bp DNA circular SYN 04-SEP-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5540)
AUTHORS Piehl M, Cassimeris L
TITLE Organization and dynamics of growing microtubule plus ends during
early mitosis.
JOURNAL Mol Biol Cell. 2003 Mar . 14(3):916-25.
PUBMED 12631713
REFERENCE 2 (bases 1 to 5540)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 4, 2026 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol
Cell. 2003 Mar . 14(3):916-25."
COMMENT Sequence Label: pGFP-EB1
FEATURES Location/Qualifiers
source 1..5540
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 16..319
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 320..523
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 474..494
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 595..611
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 595..611
/label=pBluescriptSK
/note="For pBluescript vector"
regulatory 1435..1444
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS join(1441..1443,1444..1446,1447..2160)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(1441..1443,1444..1446,1447..2157)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1486..1507)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1747..1766)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2094..2115
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 2283..2404
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2320..2339)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2374..2393
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2411..2866)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2548..2569)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2760..2779
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2893..2997
/gene="bla"
/label=AmpR promoter
promoter 2999..3356
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2999..3190
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(3022..3042)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3207..3342
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3391..4185
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 3391..4185
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3445..3464)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4055..4074
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4373..4392)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4417..4464
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4793..5381
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5282..5301
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
541 gatccgctag cgctaccgga ctcagatctc gagctccacc gcggtggcgg ccgctctaga
601 actagtggat ccatggcagt gaacgtatac tcaacgtcag tgaccagtga taacctaagt
661 cgacatgaca tgctggcctg gatcaatgag tctctgcagt tgaatctgac aaagatcgaa
721 cagttgtgct caggggctgc gtattgtcag tttatggaca tgctgttccc tggctccatt
781 gccttgaaga aagtgaaatt ccaagctaag ctagaacacg agtacatcca gaacttcaaa
841 atattacaag caggttttaa gagaatgggt gttgacaaaa taattcctgt ggacaaatta
901 gtaaaaggaa agtttcagga caattttgaa ttcgttcagt ggttcaagaa gttcttcgat
961 gcaaactatg atggaaaaga ctatgaccct gtggctgcta gacaaggtca agaaactgca
1021 gtggctcctt cccttgttgc tccagctctg aataaaccga agaaacctct cacttctagc
1081 agtgcagctc cccagaggcc catctcaaca cagagaaccg ctgcggctcc taaggctggc
1141 cctggtgtgg tgcgaaagaa ccctggtgtg ggcaacggag acgacgaggc agctgagttg
1201 atgcagcagg tcaacgtatt gaaacttact gttgaagact tggagaaaga gagggatttc
1261 tacttcggaa agctacggaa cattgaattg atttgccagg agaacgaggg ggaaaacgac
1321 cctgtattgc agaggattgt agacattctg tatgccacag atgaaggctt tgtgatacct
1381 gatgaagggg gcccacagga ggagcaagaa gagtataccc gggatccacc ggtcgccacc
1441 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
1501 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
1561 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
1621 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
1681 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
1741 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
1801 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
1861 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
1921 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
1981 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
2041 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
2101 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
2161 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
2221 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
2281 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
2341 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
2401 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
2461 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
2521 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
2581 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
2641 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
2701 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
2761 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
2821 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
2881 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
2941 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
3001 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
3061 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
3121 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
3181 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
3241 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
3301 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
3361 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
3421 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
3481 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
3541 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
3601 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
3661 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
3721 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
3781 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
3841 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
3901 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
3961 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
4021 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
4081 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
4141 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
4201 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
4261 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
4321 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
4381 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
4441 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
4501 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
4561 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
4621 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
4681 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
4741 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
4801 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
4861 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
4921 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
4981 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
5041 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
5101 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
5161 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
5221 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
5281 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
5341 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
5401 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
5461 ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg
5521 gggtcattag ttcatagccc
//
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