| 质粒名称: | pAAVLINK-DSP-B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001776 |
| 产品用途: | Body' AAVLINK plasmid for DSP expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DSP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-DSP-B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-DSP-B载体质粒基本信息
pAAVLINK-DSP-B质粒图谱载体图谱和pAAVLINK-DSP-B载体序列质粒序列多克隆位点信息
pAAVLINK-DSP-B质粒载体简介
pAAVLINK-DSP-B质粒序列载体序列
LOCUS pAAVLINK-DSP-B 6523 bp DNA circular SYN 24-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6523)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6523
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=loxJTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3679
/codon_start=1
/label=DSP
/translation="AADVHARYIELLTRSGDYYRFLSEMLKSLEDLKLKNTKIEVLEEE
LRLARDANSENCNKNKFLDQNLQKYQAECSQFKAKLASLEELKRQAELDGKSAKQNLDK
CYGQIKELNEKITRLTYEIEDEKRRRKSVEDRFDQQKNDYDQLQKARQCEKENLGWQKL
ESEKAIKEKEYEIERLRVLLQEEGTRKREYENELAKVRNHYNEEMSNLRNKYETEINIT
KTTIKEISMQKEDDSKNLRNQLDRLSRENRDLKDEIVRLNDSILQATEQRRRAEENALQ
QKACGSEIMQKKQHLEIELKQVMQQRSEDNARHKQSLEEAAKTIQDKNKEIERLKAEFQ
EEAKRRWEYENELSKVRNNYDEEIISLKNQFETEINITKTTIHQLTMQKEEDTSGYRAQ
IDNLTRENRSLSEEIKRLKNTLTQTTENLRRVEEDIQQQKATGSEVSQRKQQLEVELRQ
VTQMRTEESVRYKQSLDDAAKTIQDKNKEIERLKQLIDKETNDRKCLEDENARLQRVQY
DLQKANSSATETINKLKVQEQELTRLRIDYERVSQERTVKDQDITRFQNSLKELQLQKQ
KVEEELNRLKRTASEDSCKRKKLEEELEGMRRSLKEQAIKITNLTQQLEQASIVKKRSE
DDLRQQRDVLDGHLREKQRTQEELRRLSSEVEALRRQLLQEQESVKQAHLRNEHFQKAI
EDKSRSLNESKIEIERLQSLTENLTKEHLMLEEELRNLRLEYDDLRRGRSEADSDKNAT
ILELRSQLQISNNRTLELQGLINDLQRERENLRQEIEKFQKQALEASNRIQESKNQCTQ
VVQERESLLVKIKVLEQDKARLQRLEDELNRAKSTLEAETRVKQRLECEKQQIQNDLNQ
WKTQYSRKEEAIRKIESEREKSEREKNSLRSEIERLQAEIKRIEERCRRKLEDSTRETQ
SQLETERSRYQREIDKLRQRPYGSHRETQTECEWTVDTSKLVFDGLRKKVTAMQLYECQ
LIDKTTLDKLLKGKKSVEEVASEIQPFLRGAGSIAGASASPKEKYSLVEAKRKKLISPE
STVMLLEAQAATGGIIDPHRNEKLTVDSAIARDLIDFDDRQQIYAAEKAITGFDDPFSG
KTVSVSEAIKKNLIDRETGMRLLEAQIAS"
misc_feature 1226..1231
/label=SDS
misc_feature 1289..1294
/label=SDS
misc_feature 1292..1297
/label=SDS
misc_feature 1555..1560
/label=SDS
misc_feature 2089..2094
/label=SDS
misc_feature 2248..2253
/label=SDS
misc_feature 2906..2911
/label=SDS
misc_feature 2909..2914
/label=SDS
intron 3680..3720
/label=Splicing Donor
misc_feature 3724..3757
/label=lox2272-JT1571
repeat_region 3786..3926
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3786..3915
/label=AAV2 ITR
rep_origin 4001..4456
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4738..4842
/gene="bla"
/label=AmpR promoter
CDS 4843..5703
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5874..6462
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagca tacattatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gctgcagatg ttcatgctcg gtacattgaa
301 ctacttacaa gatctggaga ctattacagg ttcttaagtg agatgctgaa gagtttggaa
361 gatctgaagc tgaaaaatac caagatcgaa gttttggaag aggagctcag actggcccga
421 gatgccaact cggaaaactg taataagaac aaattcctgg atcagaacct gcagaaatac
481 caggcagagt gttcccagtt caaagcgaag cttgcgagcc tggaggagct gaagagacag
541 gctgagctgg atgggaagtc ggctaagcaa aatctagaca agtgctacgg ccaaataaaa
601 gaactcaatg agaagatcac ccgactgact tatgagattg aagatgaaaa gagaagaaga
661 aaatctgtgg aagacagatt tgaccaacag aagaatgact atgaccaact gcagaaagca
721 aggcaatgtg aaaaggagaa ccttggttgg cagaaattag agtctgagaa agccatcaag
781 gagaaggagt acgagattga aaggttgagg gttctactgc aggaagaagg cacccggaag
841 agagaatatg aaaatgagct ggcaaaggta agaaaccact ataatgagga gatgagtaat
901 ttaaggaaca agtatgaaac agagattaac attacgaaga ccaccatcaa ggagatatcc
961 atgcaaaaag aggatgattc caaaaatctt agaaaccagc ttgatagact ttcaagggaa
1021 aatcgagatc tgaaggatga aattgtcagg ctcaatgaca gcatcttgca ggccactgag
1081 cagcgaaggc gagctgaaga aaacgccctt cagcaaaagg cctgtggctc tgagataatg
1141 cagaagaagc agcatctgga gatagaactg aagcaggtca tgcagcagcg ctctgaggac
1201 aatgcccggc acaagcagtc cctggaggag gctgccaaga ccattcagga caaaaataag
1261 gagatcgaga gactcaaagc tgagtttcag gaggaggcca agcgccgctg ggaatatgaa
1321 aatgaactga gtaaggtaag aaacaattat gatgaggaga tcattagctt aaaaaatcag
1381 tttgagaccg agatcaacat caccaagacc accatccacc agctcaccat gcagaaggaa
1441 gaggatacca gtggctaccg ggctcagata gacaatctca cccgagaaaa caggagctta
1501 tctgaagaaa taaagaggct gaagaacact ctaacccaga ccacagagaa tctcaggagg
1561 gtggaagaag acatccaaca gcaaaaggcc actggctctg aggtgtctca gaggaaacag
1621 cagctggagg ttgagctgag acaagtcact cagatgcgaa cagaggagag cgtaagatat
1681 aagcaatctc ttgatgatgc tgccaaaacc atccaggata aaaacaagga gatagaaagg
1741 ttaaaacaac tgatcgacaa agaaacaaat gaccggaaat gcctggaaga tgaaaacgcg
1801 agattacaaa gggtccagta tgacctgcag aaagcaaaca gtagtgcgac ggagacaata
1861 aacaaactga aggttcagga gcaagaactg acacgcctga ggatcgacta tgaaagggtt
1921 tcccaggaga ggactgtgaa ggaccaggat atcacgcggt tccagaactc tctgaaagag
1981 ctgcagctgc agaagcagaa ggtggaagag gagctgaatc ggctgaagag gaccgcgtca
2041 gaagactcct gcaagaggaa gaagctggag gaagagctgg aaggcatgag gaggtcgctg
2101 aaggagcaag ccatcaaaat caccaacctg acccagcagc tggagcaggc atccattgtt
2161 aagaagagga gtgaggatga cctccggcag cagagggacg tgctggatgg ccacctgagg
2221 gaaaagcaga ggacccagga agagctgagg aggctctctt ctgaggtcga ggccctgagg
2281 cggcagttac tccaggaaca ggaaagtgtc aaacaagctc acttgaggaa tgagcatttc
2341 cagaaggcga tagaagataa aagcagaagc ttaaatgaaa gcaaaataga aattgagagg
2401 ctgcagtctc tcacagagaa cctgaccaag gagcacttga tgttagaaga agaactgcgg
2461 aacctgaggc tggagtacga tgacctgagg agaggacgaa gcgaagcgga cagtgataaa
2521 aatgcaacca tcttggaact aaggagccag ctgcagatca gcaacaaccg gaccctggaa
2581 ctgcaggggc tgattaatga tttacagaga gagagggaaa atttgagaca ggaaattgag
2641 aaattccaaa agcaggcttt agaggcatct aataggattc aggaatcaaa gaatcagtgt
2701 actcaggtgg tacaggaaag agagagcctt ctggtgaaaa tcaaagtcct ggagcaagac
2761 aaggcaaggc tgcagaggct ggaggatgag ctgaatcgtg caaaatcaac tctagaggca
2821 gaaaccaggg tgaaacagcg cctggagtgt gagaaacagc aaattcagaa tgacctgaat
2881 cagtggaaga ctcaatattc ccgcaaggag gaggctatta ggaagataga atcggaaaga
2941 gaaaagagtg agagagagaa gaacagtctt aggagtgaga tcgaaagact ccaagcagag
3001 atcaagagaa ttgaagagag gtgcaggcgt aagctggagg attctaccag ggagacacag
3061 tcacagttag aaacagaacg ctcccgatat cagagggaga ttgataaact cagacagcgc
3121 ccatatgggt cccatcgaga gacccagact gagtgtgagt ggaccgttga cacctccaag
3181 ctggtgtttg atgggctgag gaagaaggtg acagcaatgc agctctatga gtgtcagctg
3241 atcgacaaaa caaccttgga caaactattg aaggggaaga agtcagtgga agaagttgct
3301 tctgaaatcc agccattcct tcggggtgca ggatctatcg ctggagcatc tgcttctcct
3361 aaggaaaaat actctttggt agaggccaag agaaagaaat taatcagccc agaatccaca
3421 gtcatgcttc tggaggccca ggcagctaca ggtggtataa ttgatcccca tcggaatgag
3481 aagctgactg tcgacagtgc catagctcgg gacctcattg acttcgatga ccgtcagcag
3541 atatatgcag cagaaaaagc tatcactggt tttgatgatc cattttcagg caagacagta
3601 tctgtttcag aagccatcaa gaaaaatttg attgatagag aaaccggaat gcgcctgctg
3661 gaagcccaga ttgcttcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
3721 agctattatt cgtataggat actttatacg aagttatgaa ttccacgtgc ggaccgagcg
3781 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
3841 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
3901 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
3961 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
4021 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
4081 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
4141 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
4201 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
4261 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
4321 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
4381 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
4441 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
4501 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
4561 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
4621 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
4681 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
4741 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
4801 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
4861 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
4921 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
4981 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
5041 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
5101 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
5161 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
5221 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
5281 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
5341 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
5401 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
5461 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
5521 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
5581 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
5641 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
5701 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
5761 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
5821 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
5881 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
5941 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
6001 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
6061 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
6121 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
6181 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
6241 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
6301 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
6361 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
6421 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
6481 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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