pSPYCE35S-bk1-YFP-N-bk2

LOCUS       pSPYCE35S-bk1-YFP-N-bk2              4374 bp DNA     circular SYN 31-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Youbio
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..42
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        566..911
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     852..871
                     /label=YNEF
     promoter        920..922
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             968..970
                     /codon_start=1
                     /label=Feature 10
                     /translation="M"
     CDS             971..1000
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    1001..1465
                     /label=YFP-N
     primer_bind     complement(1046..1067)
                     /label=pEGFP-N-3
     misc_feature    1384..1402
                     /label=YFP-N-F
     misc_feature    1472..1492
                     /label=同源臂
     misc_feature    1505..1525
                     /label=同源臂
     terminator      1539..1791
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1800..1816)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2290..2394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2395..3255
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3426..4014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
       61 gcatgcctgc aggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
      121 tggttagaga ggcttacgca gcaggtctca tcaagacgat ctacccgagc aataatctcc
      181 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      241 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      301 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
      361 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
      421 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
      481 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      541 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      601 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      661 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      721 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      781 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      841 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      901 tggagagaac acgggggacc aggtcgactc tcgaggatcc ggtacccggt atcgatttaa
      961 aaggcctatg gagcaaaagt tgatttctga ggaggatctt atggtgagca agggcgagga
     1021 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1081 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1141 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     1201 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1261 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1321 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1381 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1441 cagccacaac gtctatatca tggccaggcc tcaggtcgac tctcgaggat caactagtga
     1501 gctcagtacc cggtatcgat ttaaataaga atttccccga tcgttcaaac atttggcaat
     1561 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     1621 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     1681 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     1741 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattca
     1801 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1861 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1921 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     1981 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     2041 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     2101 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     2161 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     2221 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     2281 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2341 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2401 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2461 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2521 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2581 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2641 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2701 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2761 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2821 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2881 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2941 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3001 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3061 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3121 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3181 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3241 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3301 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3361 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3421 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3481 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3541 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3601 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3661 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3721 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3781 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3841 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3901 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3961 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4021 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4081 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4141 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4201 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4261 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     4321 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tgga
//