| 质粒名称: | pAAVLINK-EF1alpha-HA-5'-CUL7 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001856 |
| 产品用途: | 5' AAVLINK plasmid for CUL7 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CUL7 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-HA-5'-CUL7
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-HA-5'-CUL7载体质粒基本信息
pAAVLINK-EF1alpha-HA-5'-CUL7质粒图谱载体图谱和pAAVLINK-EF1alpha-HA-5'-CUL7载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-HA-5'-CUL7质粒载体简介
pAAVLINK-EF1alpha-HA-5'-CUL7质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-HA-5'-CUL7 7055 bp DNA circular SYN 24-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7055)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 10, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7055
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..4195
/codon_start=1
/gene="CUL7"
/gene_synonym="3M1; CUL-7; dJ20C7.5; KIAA0076"
/product="cullin-7 isoform 2"
/label=CUL7
/note="isoform 2 is encoded by transcript variant 2"
/db_xref="GI:41872646"
/db_xref="CCDS:CCDS4881.1"
/db_xref="GeneID:9820"
/db_xref="HGNC:HGNC:21024"
/db_xref="MIM:609577"
/protein_id="NP_055595.2"
/translation="VGELRYREFRVPLGPGLHAYPDELIRQRVGHDGHPEYQIRWLILR
RGDEGDGGSGQVDCKAEHILLWMSKDEIYANCHKMLGEDGQVIGPSQESAGEVGALDKS
VLEEMETDVKSLIQRALRQLEECVGTIPPAPLLHTVHVLSAYASIEPLTGVFKDPRVLD
LLMHMLSSPDYQIRWSAGRMIQALSSHDAGTRTQILLSLSQQEAIEKHLDFDSRCALLA
LFAQATLSEHPMSFEGIQLPQVPGRVLFSLVKRYLHVTSLLDQLNDSAAEPGAQNTSAP
EELSGERGQLELEFSMAMGTLISELVQAMRWDQASDRPRSSARSPGSIFQPQLADVSPG
LPAAQAQPSFRRSRRFRPRSEFASGNTYALYVRDTLQPGMRVRMLDDYEEISAGDEGEF
RQSNNGVPPVQVFWESTGRTYWVHWHMLEILGFEEDIEDMVEADEYQGAVASRVLGRAL
PAWRWRPMTELYAVPYVLPEDEDTEECEHLTLAEWWELLFFIKKLDGPDHQEVLQILQE
NLDGEILDDEILAELAVPIELAQDLLLTLPQRLNDSALRDLINCHVYKKYGPEALAGNQ
AYPSLLEAQEDVLLLDAQAQAKDSEDAAKVEAKEPPSQSPNTPLQRLVEGYGPAGKILL
DLEQALSSEGTQENKVKPLLLQLQRQPQPFLALMQSLDTPETNRTLHLTVLRILKQLVD
FPEALLLPWHEAVDACMACLRSPNTDREVLQELIFFLHRLTSVSRDYAVVLNQLGARDA
ISKALEKHLGKLELAQELRDMVFKCEKHAHLYRKLITNILGGCIQMVLGQIEDHRRTHR
PINIPFFDVFLRYLCQGSSVEVKEDKCWEKVEVSSNPHRASKLTDHNPKTYWESNGSAG
SHYITLHMRRGILIRQLTLLVASEDSSYMPARVVVCGGDSTSSLHTELNSVNVMPSASR
VILLENLTRFWPIIQIRIKRCQQGGIDTRIRGLEILGPKPTFWPVFREQLCRHTRLFYM
VRAQAWSQDMAEDRRSLLHLSSRLNGALRQEQNFADRFLPDDEAAQALGKTCWEALVSP
VVQNITSPDEDGISPLGWLLDQYLECQEAVFNPQSRGPAFFSRVRRLTHLLVHVEPCEA
PPPVVATPRPKGRNRSHDWSSLATRGLPSSIMRNLTRCWRAVVEKQ"
misc_feature 1130..1135
/label=SDS
misc_feature 1779..1784
/label=SDS
misc_feature 1787..1792
/label=SDS
misc_feature 1955..1960
/label=SDS
misc_feature 2262..2267
/label=SDS
misc_feature 2500..2505
/label=SDS
misc_feature 2635..2640
/label=SDS
misc_feature 3240..3245
/label=SDS
misc_feature 3532..3537
/label=SDS
misc_feature 3751..3756
/label=SDS
misc_feature 4061..4066
/label=SDS
intron 4196..4236
/label=Splicing Donor
misc_feature 4240..4273
/label=lox2272-JT1571
repeat_region 4318..4458
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4318..4447
/label=AAV2 ITR
repeat_region 4319..4459
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4533..4988
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5270..5374
/gene="bla"
/label=AmpR promoter
CDS 5375..6235
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6406..6994
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgtgg gagaactccg ctacagggaa ttcagggtgc ccctggggcc
781 cggcttacat gcctatcctg atgagctgat ccgccagcgc gtgggccatg atgggcatcc
841 tgagtaccag atccgttggc tcatcctgcg gcgtggcgat gagggggacg ggggctctgg
901 ccaagtggac tgcaaggctg agcacatcct gctgtggatg tccaaggatg agatctatgc
961 caactgccac aagatgctgg gcgaggatgg ccaggtcatc gggccctccc aggagtctgc
1021 aggggaggtt ggggccctgg acaaatctgt gctggaggag atggaaaccg atgtgaagtc
1081 cctcattcag agagcccttc ggcagctgga ggagtgtgtg ggcactatcc ctcctgctcc
1141 tctacttcac actgtccacg tgctcagcgc ctatgccagc attgagcccc tcactggagt
1201 attcaaggac ccaagggtcc tggacttgct catgcacatg ttgagtagtc ctgattatca
1261 gattcgctgg agtgcaggcc ggatgataca agccctgtcc tcccatgacg ctgggacccg
1321 gactcagatc cttctgtcac tgagccaaca agaagccatt gagaaacacc tggattttga
1381 cagccgctgt gctctgctag cactgtttgc acaggccacg ctctctgaac accccatgtc
1441 tttcgagggc attcagctac cacaggtccc aggaagggtg ctcttctccc tggtgaagcg
1501 gtatttgcat gtcacctcgc tcctggatca gctgaacgac agtgctgcgg agccaggagc
1561 ccagaacacc tctgctcctg aggagttgag tggggagagg ggtcaactgg agctggagtt
1621 cagtatggcc atgggcaccc tgatctcgga gctggtgcaa gccatgcgct gggaccaggc
1681 ctcagacaga ccaaggagct cagcacggtc ccccggttcc atcttccagc ctcagctggc
1741 agatgtgagc ccagggctcc ccgctgccca ggctcagccc tccttcagga ggtcaagacg
1801 ttttcgccct cgttctgagt tcgcaagtgg caatacctat gctttgtatg tgcgggacac
1861 actgcagccg gggatgcgag tgcggatgct ggatgattat gaggagatca gtgccgggga
1921 tgagggcgag tttcggcaga gcaacaacgg tgtgcctcct gtgcaggtat tttgggagtc
1981 aacaggccgc acctattggg tgcactggca catgctggag atcttgggct ttgaggaaga
2041 cattgaggac atggttgagg ctgatgagta ccaaggggca gtggccagta gagtcctggg
2101 tagagccctg cctgcctggc gctggaggcc catgacagaa ctctatgctg tgccttatgt
2161 gctgcctgag gatgaggaca ctgaggagtg tgaacacctg accctggctg agtggtggga
2221 actcctcttc ttcatcaaga agctggatgg acctgaccat caggaggttc tccagatcct
2281 ccaggagaac ctagatgggg agattctgga tgatgagatc ctagctgaac tggccgtgcc
2341 catagaattg gcccaggact tgctgctgac tctgccacag cgactcaatg acagtgccct
2401 cagggacctg atcaactgcc atgtctacaa gaagtatggg cctgaagccc tagcagggaa
2461 ccaagcctac ccatcccttc tagaagccca agaagatgtc ctcctgctag acgcgcaggc
2521 ccaggctaag gactcagaag atgcagccaa agtggaagca aaagaacccc catctcagag
2581 tcccaacact cccctgcagc gtctggtgga gggttatggt ccagctggga aaatcctcct
2641 ggatctagag caagccctca gctcagaggg gacccaggag aacaaggtca agccactcct
2701 gctgcagctg cagcggcagc cgcagccctt cctggcactg atgcagagcc tggacactcc
2761 ggagactaac aggaccctgc acctgactgt gctgagaatc ctgaagcagc tggtggactt
2821 ccccgaggca ctgctgctcc cctggcacga ggccgtggat gcctgcatgg cctgcctgcg
2881 gtccccaaac actgatcgag aggtgctcca ggaactgatt ttcttcctgc accgcctgac
2941 ctcagtgagc agggactatg ccgtggtgct gaatcagctg ggagcaagag acgctatctc
3001 caaggccctg gaaaagcacc tgggaaagct ggagctggct caggagctgc gggacatggt
3061 gttcaagtgt gagaagcatg cccacctcta ccgcaaactc atcaccaaca tcctgggagg
3121 ctgcatccag atggtgctgg gccagatcga agaccacaga cgaacccacc ggcccatcaa
3181 catccctttc tttgatgtgt tcctcagata cctgtgccag ggctccagtg tggaagtgaa
3241 ggaggacaag tgctgggaga aggtggaggt gtcctccaac ccgcaccggg ccagcaagct
3301 gacggaccac aaccccaaga cctattggga gtccaacggc agcgccggct cccactacat
3361 caccctgcac atgcgccggg gcatcctcat caggcaactg actctgcttg tggctagtga
3421 ggactcgagt tacatgccgg cccgagtggt ggtgtgcggg ggtgatagca ctagctctct
3481 tcacacggaa ctcaactcgg tgaatgtgat gccctctgcc agccgggtga tcctcctgga
3541 gaacctgacc cgcttctggc ccatcatcca gatccgcata aagcgctgcc agcagggtgg
3601 cattgatacg cgcattcggg ggttagagat cctaggcccc aagcccacgt tctggccagt
3661 gttccgggag cagctctgtc gtcacacacg cctcttctac atggttcggg cacaggcctg
3721 gagccaggac atggcagagg accgcaggag cctcctgcac ctgagttcta gactcaacgg
3781 tgctctgcgc caggagcaga attttgctga ccgcttcctc cctgatgacg aggctgccca
3841 agctctgggc aagacctgct gggaggccct ggtcagcccc gtggtgcaga acatcacctc
3901 ccctgatgag gatggcatta gccccctggg ttggctgctg gaccagtacc tggagtgtca
3961 ggaagctgtc ttcaaccccc agagccgcgg cccagctttc ttctcgcggg tgcgccgtct
4021 cactcacctg ctggtgcatg tcgagccctg tgaggcaccc cctcctgtgg tggccactcc
4081 tcggcccaaa ggcagaaaca gaagccacga ctggagctcc ttggctaccc ggggccttcc
4141 aagcagcatc atgagaaacc tgacgcgctg ttggcgggcc gtggtggaga agcaggtaag
4201 tatcaaggtt acaagacagg tttaaggaga ccaataagct attattcgta taggatactt
4261 tatacgaagt tattaagtta tggagaccgg tgtggcacgt gcggaccgag cggccgcagg
4321 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
4381 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
4441 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
4501 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
4561 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
4621 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
4681 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
4741 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
4801 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
4861 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
4921 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
4981 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
5041 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
5101 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
5161 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
5221 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
5281 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
5341 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
5401 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
5461 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
5521 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
5581 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
5641 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
5701 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
5761 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
5821 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
5881 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
5941 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
6001 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
6061 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
6121 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
6181 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
6241 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
6301 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
6361 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
6421 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
6481 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
6541 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
6601 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
6661 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
6721 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
6781 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
6841 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
6901 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
6961 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
7021 gttcctggcc ttttgctggc cttttgctca catgt
//
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