| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYH9-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001586 |
| 产品用途: | 3' AAVLINK plasmid for MYH9 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MYH9 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYH9-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYH9-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYH9-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYH9-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYH9-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYH9-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYH9-FLAG 6723 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6723)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6723
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3428
/codon_start=1
/label=MYH9
/translation="VEDEKNSFREQLEEEEEAKHNLEKQIATLHAQVADMKKKMEDSVG
CLETAEEVKRKLQKDLEGLSQRHEEKVAAYDKLEKTKTRLQQELDDLLVDLDHQRQSAC
NLEKKQKKFDQLLAEEKTISAKYAEERDRAEAEAREKETKALSLARALEEAMEQKAELE
RLNKQFRTEMEDLMSSKDDVGKSVHELEKSKRALEQQVEEMKTQLEELEDELQATEDAK
LRLEVNLQAMKAQFERDLQGRDEQSEEKKKQLVRQVREMEAELEDERKQRSMAVAARKK
LEMDLKDLEAHIDSANKNRDEAIKQLRKLQAQMKDCMRELDDTRASREEILAQAKENEK
KLKSMEAEMIQLQEELAAAERAKRQAQQERDELADEIANSSGKGALALEEKRRLEARIA
QLEEELEEEQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELK
VKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQ
VDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADA
MNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE"
misc_feature 1584..1589
/label=SDS
misc_feature 1587..1592
/label=SDS
misc_feature 1590..1595
/label=SDS
misc_feature 1593..1598
/label=SDS
misc_feature 1698..1703
/label=SDS
misc_feature 2604..2609
/label=SDS
misc_feature 2751..2756
/label=SDS
misc_feature 2763..2768
/label=SDS
misc_feature 3186..3191
/label=SDS
misc_feature 3198..3203
/label=SDS
CDS 3431..3496
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3512..3943
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3656..3661
/label=SDS
repeat_region 3986..4126
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3986..4115
/label=AAV2 ITR
rep_origin 4201..4656
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4938..5042
/gene="bla"
/label=AmpR promoter
CDS 5043..5903
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6074..6662
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgg aggacgagaa
1561 gaattccttc cgggagcagc tggaggagga ggaggaggcc aagcacaacc tggagaagca
1621 gatcgccacc ctccatgccc aggtggccga catgaaaaag aagatggagg acagtgtggg
1681 gtgcctggaa actgctgagg aggtgaagag gaagctccag aaggacctgg agggcctgag
1741 ccagcggcac gaggagaagg tggccgccta cgacaagctg gagaagacca agacgcggct
1801 gcagcaggag ctggacgacc tgctggtgga cctggaccac cagcgccaga gcgcgtgcaa
1861 cctggagaag aagcagaaga agtttgacca gctcctggcg gaggagaaga ccatctctgc
1921 caagtatgca gaggagcgcg accgggctga ggcggaggcc cgagagaagg agaccaaggc
1981 tctgtcgctg gcccgggccc tggaggaagc catggagcag aaggcggagc tggagcggct
2041 caacaagcag ttccgcacgg agatggagga ccttatgagc tccaaggatg atgtgggcaa
2101 gagtgtccac gagctggaga agtccaagcg ggccctagag cagcaggtgg aggagatgaa
2161 gacgcagctg gaagagctgg aggacgagct gcaggccacc gaagatgcca agctgcggtt
2221 ggaggtcaac ctgcaggcca tgaaggccca gttcgagcgg gacctgcagg gccgggacga
2281 gcagagcgag gagaagaaga agcagctggt cagacaggtg cgggagatgg aggcagagct
2341 ggaggacgag aggaagcagc gctcgatggc agtggccgcc cggaagaagc tggagatgga
2401 cctgaaggac ctggaggcgc acatcgactc ggccaacaag aaccgggacg aagccatcaa
2461 acagctgcgg aagctgcagg cccagatgaa ggactgcatg cgcgagctgg atgacacccg
2521 cgcctctcgt gaggagatcc tggcccaggc caaagagaac gagaagaagc tgaagagcat
2581 ggaggccgag atgatccagt tgcaggagga actggcagcc gcggagcgtg ccaagcgcca
2641 ggcccagcag gagcgggatg agctggctga cgagatcgcc aacagcagcg gcaaaggagc
2701 cctggcgtta gaggagaagc ggcgtctgga ggcccgcatc gcccagctgg aggaggagct
2761 ggaggaggag cagggcaaca cggagctgat caacgaccgg ctgaagaagg ccaacctgca
2821 gatcgaccag atcaacaccg acctgaacct ggagcgcagc cacgcccaga agaacgagaa
2881 tgctcggcag cagctggaac gccagaacaa ggagcttaag gtcaagctgc aggagatgga
2941 gggcactgtc aagtccaagt acaaggcctc catcaccgcc ctcgaggcca agattgcaca
3001 gctggaggag cagctggaca acgagaccaa ggagcgccag gcagcctgca aacaggtgcg
3061 tcggaccgag aagaagctga aggatgtgct gctgcaggtg gatgacgagc ggaggaacgc
3121 cgagcagtac aaggaccagg ccgacaaggc atctacccgc ctgaagcagc tcaagcggca
3181 gctggaggag gccgaagagg aggcccagcg ggccaacgcc tcccgccgga aactgcagcg
3241 cgagctggag gacgccactg agacggccga tgccatgaac cgcgaagtca gctccctaaa
3301 gaacaagctc aggcgcgggg acctgccgtt tgtcgtgccc cgccgaatgg cccggaaagg
3361 cgccggggat ggctccgacg aagaggtaga tggcaaagcg gatggggctg aggccaaacc
3421 tgccgaaaag gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
3481 ggatgacgat gacaagtaag aattctgatg actcgagata atcaacctct ggattacaaa
3541 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
3601 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
3661 ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc
3721 tgctggacag gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt
3781 tgtgatgcta ttgctttatt tgtaaccatc tagctttatt tgtgaaattt gtgatgctat
3841 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
3901 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagatctg gagaccggtg
3961 tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct
4021 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
4081 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg
4141 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
4201 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
4261 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
4321 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
4381 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
4441 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
4501 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat
4561 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta
4621 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
4681 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
4741 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
4801 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
4861 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
4921 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
4981 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
5041 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
5101 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
5161 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
5221 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
5281 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
5341 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
5401 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
5461 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
5521 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
5581 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
5641 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
5701 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
5761 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
5821 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
5881 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
5941 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
6001 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
6061 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
6121 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
6181 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
6241 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
6301 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
6361 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
6421 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
6481 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
6541 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
6601 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
6661 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
6721 tgt
//
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