| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-TANC2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001423 |
| 产品用途: | 3' AAVLINK plasmid for TANC2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TANC2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-TANC2-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-TANC2-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-TANC2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-TANC2-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-TANC2-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-TANC2-FLAG 7410 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7410)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7410
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..4114
/codon_start=1
/label=TANC2
/translation="VPPLLLWTKKD*QPSAGLV*RAISQ*YVLWWITELPQTMLTRMAV
PHWIWQLSMAMLRWSSSW*IMGP*SSTLTTVECALWIGQWGAGTLLLLSLF*RKEPR*V
VRRYRVAHEVQPHGRWPPPSQTS*SSC*AS*WKRGTCFIRKVK*RKLPSATSTP*RSSL
EKGLVRT*KLSGN*RCLSSSTSLGVAGK*TILEWRRNLLLRPWS*NRNLMKLTMREQGQ
NAAADSSQQP*RT*TRPSSCVPTTVRSRDFC*EWKKSVDRCSSHSSHRRHRSLSSSCRK
KQNLSHSMKTYTLYRIYSRRSTWNRMLKMFPLASRQRPGPARGSRSSRAHPPLPRIGTQ
PTSPAHLLALIRFLTSGPVVL*ALPLDRPISPPHLPFLQLIRTHITGLAHHTLPRLIRE
DLTVSAPLLWEDRAKNTQALPLPLSGEALSIGPALQLKV*VSIDPSLVHPCAISRKQAS
VSFLADPNLHYPKWPSGPTRCLSSLWQFPSKGSGYSLPRPRL*EVTSPAQPSIQAPSSP
QEPMAK*PIQWPVNTSLHKET*ESARAGWFIKGQLGES*GMEGRCSMSKPA*VQAPSVS
MED*PKRIFHSDLPQHTEVA*DTARHHRSDAASQHPITQSVTQN*IWSAPPAN*VPLMC
RI*SEDLSVSTLTKSNRTRQLPGRCCIPKV*AFASLHLAIVSPPPPT*LRPSGHLLPSS
KWRSH*NLHMRGHVTSCRQCLQLKEVTPVSPPDPGPHHSWGS*IKQHGLSSTPTSTSRI
GPGQCHLWTPSSVPRLQATCLSLSPSVHHHPSATLPFITKPTMHRMAICWRTIITAPMG
CWLTGLVETSWSESARPPPIPT*R*LGLYLWLRHTRTTCTGSCPETLGKGRHPLSNQRD
RSWSLM"
misc_feature 2023..2028
/label=SDS
misc_feature 2379..2384
/label=SDS
misc_feature 2475..2480
/label=SDS
misc_feature 2699..2704
/label=SDS
misc_feature 3634..3639
/label=SDS
misc_feature 3966..3971
/label=SDS
misc_feature 3969..3974
/label=SDS
CDS 4118..4183
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4199..4630
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4343..4348
/label=SDS
repeat_region 4673..4813
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4673..4802
/label=AAV2 ITR
rep_origin 4888..5343
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5625..5729
/gene="bla"
/label=AmpR promoter
CDS 5730..6590
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6761..7349
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgcctcca ttgctcttat
1501 ggacaaagaa ggattgacag ccctcagctg ggcttgtttg aagggccatc tctcagtagt
1561 acgttctctg gtggataacg gagctgccac agaccatgct gacaagaatg gccgtacccc
1621 actggatctg gcagctttct atggcgatgc tgaggtggtc cagttcctgg tagatcatgg
1681 ggccatgatc gagcacgttg actacagtgg aatgcgccct ttggataggg cagtggggtg
1741 ccggaacact tctgttgttg tcactcttct gaagaaagga gccaagatag gttgtcagac
1801 gttaccgagt cgcccacgag gtccagccac atgggcgatg gccacctcca agccagacat
1861 catgatcatc ctgttgagca agctgatgga agagggggac atgttttata agaaaggtaa
1921 agtaaaggaa gctgcccagc gctaccagta cgccctgaag aagttcccta gagaagggtt
1981 tggtgaggac ttgaaaactt tccgggaact aaaggtgtct ctcctcctca acctctctcg
2041 gtgtcgcagg aaaatgaacg attttggaat ggcggaggaa tttgctacta aggccctgga
2101 gctgaaaccg aaatcttatg aagcttacta tgcgagagca agggcaaaac gcagcagcag
2161 acagttcgca gcagccttag aggacctgaa cgaggccatc aagctgtgtc ccaacaaccg
2221 tgagatccag agacttctgc tgagagtgga agaagagtgt agacagatgc agcagccaca
2281 gcagccaccg ccgccaccgc agcctcagca gcagttgccg gaagaagcag aacctgagcc
2341 acagcatgaa gacatatact ctgtacagga tatattcgag gaggagtacc tggaacagga
2401 tgttgaaaat gtttccattg gcctccagac agaggcccgg cccagccagg ggctcccggt
2461 catccagagc ccaccctcct ctcccccgca tcgggactca gcctacatct ccagctcacc
2521 tcttggctct catcaggttt ttgacttccg gtccagtagt tctgtaggct ctcccactag
2581 acagacctat cagtccacct cacctgccct ttctccaact catcagaact cacattacag
2641 gcctagccca ccacacactt ccccggctca tcagggagga tcttaccgtt tcagcccccc
2701 tcctgtggga ggacagggca aagaataccc aagccctccc ccttcccctc tccggagagg
2761 ccctcagtat cgggccagcc ctccagctga aagtatgagt gtctatagat cccagtctgg
2821 ttcacccgtg cgctatcagc aggaaacaag cgtcagtcag cttcctggca gacccaaatc
2881 tccattatcc aaaatggccc agcggcccta ccagatgcct cagctccctg tggcagttcc
2941 ccagcaaggg ctcaggctac agcctgccaa ggcccagatt gtgagaagta accagcccag
3001 cccagccgtc cattcaagca ccgtcatccc cacaggagcc tatggccaag tagcccattc
3061 aatggccagt aaataccagt cttcacaagg agacatagga gtcagccaga gccggttggt
3121 ttatcaaggg tcaattgggg gaatcgtagg ggatggaagg ccggtgcagc atgtccaagc
3181 cagcctgagt gcaggcgcca tctgtcagca tggaggattg accaaagagg atcttccaca
3241 gcgaccttcc tcagcatacc gaggtggcgt gagatacagc cagacaccac agatcggacg
3301 cagccagtca gcatcctatt acccagtctg tcactcaaaa ctagatctgg agcgctcctc
3361 cagccaacta ggttcccctg atgtgtcgca tttaatcaga agacctatca gtgtcaaccc
3421 taacgaaatc aaaccgcacc cgccaactcc caggccgttg ctgcattccc aaagtgtagg
3481 ccttcgcttc tctccatcta gcaatagtat ctcctccacc tccaacctaa ctccgacctt
3541 ccggccatct tcttccatcc agcaaatgga gatcccactg aaacctgcat atgagaggtc
3601 atgtgacgag ctgtcgccag tgtctccaac tcaaggaggt taccccagtg agcccacccg
3661 atccaggacc acaccattca tggggatcat agataaaaca gcacggactc agcagtaccc
3721 ccacctccac cagcagaatc ggacctgggc agtgtcatct gtggacaccg tcctcagtcc
3781 cacgtctcca ggcaacctgc ctcagcctga gtccttcagt ccaccatcat ccatcagcaa
3841 cattgccttt tataacaaaa ccaacaatgc acagaatggc catttgctgg aggacgatta
3901 ttacagcccc catgggatgc tggctaacgg gtctcgtgga gacctcttgg agcgagtcag
3961 ccaggcctcc tcctatcccg acgtgaaggt agctcggact ctacctgtgg ctcaggcata
4021 ccaggacaac ctgtacaggc agctgtcccg agactctcgg caagggcaga catcccctat
4081 caaaccaaag agaccgttcg tggagtctaa tgttaaggac tacaaagacc atgacggtga
4141 ttataaagat catgacatcg actacaagga tgacgatgac aagtaagaat tctgatgact
4201 cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
4261 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
4321 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
4381 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
4441 aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag
4501 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
4561 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
4621 ggttttttaa agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc
4681 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
4741 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
4801 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
4861 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
4921 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
4981 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
5041 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
5101 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
5161 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
5221 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
5281 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
5341 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
5401 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
5461 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
5521 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
5581 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
5641 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
5701 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
5761 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
5821 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
5881 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
5941 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
6001 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
6061 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
6121 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
6181 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
6241 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
6301 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
6361 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
6421 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
6481 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
6541 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
6601 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
6661 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
6721 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
6781 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
6841 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
6901 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
6961 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
7021 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
7081 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
7141 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
7201 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
7261 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
7321 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
7381 tggccttttg ctggcctttt gctcacatgt
//
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